• Korean Journal of Clinical Laboratory Science
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• v.52 no.4
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• pp.389-397
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• 2020

Microglial cells function as major immune cells in the brain, playing an important role in the protection and damage of neurons. BV2 microglia, activated by drug stimulation, secrete inflammatory cytokines by activating the nuclear factor kappa-light-chain-enhancer of the activated B cells pathway and are involved in neuroinflammatory and immune responses. The overactivation of microglia by stimuli can cause neuronal damage, leading to brain disease. Noni, a natural product, reduces the activity of microglia to prevent neuronal damage and is a potential natural medicine because it exerts excellent regeneration and anti-inflammatory effects on damaged cells. In this study, when noni was used to treat BV2 cells stimulated by the anti-cancer drug doxorubicin, it reduced the release of pro-inflammatory cytokines from BV2. On the other hand, neuronal damage is a side effect of doxorubicin. Therefore, the cytokines released from doxorubicin-stimulated BV2 cells treated with noni had a positive effect on the neuronal viability compared to those released from doxorubicin-stimulated BV2 cells not treated with Noni. Thus, Noni increases neuronal viability. These results suggest that noni inhibits the release of cytokines by regulating the nuclear factor kappa-light-chain-enhancer of the activated B cells pathway of BV2, thereby inhibiting neuronal damage.

• Proceedings of the Korean Society of Computer Information Conference
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• 2021.07a
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• pp.355-357
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• 2021

환자들은 병원을 방문하면 정확한 진단 정보를 얻기 위하여 채혈한다. 이때 채혈은 주로 임상병리사가 시행하는데 채혈 시 실수가 발생하면 감염으로부터 위험해질 수 있다. 따라서 채혈에 따른 주삿바늘로부터의 감염을 예방하고 환자와 임상병리사의 안전을 위한 올인원 채혈기를 고안했다. 올인원 채혈기는 가장 굵은 정맥혈관을 찾기 위해 'AccuVein'의 기능을 이용했다. 이에 본 올인원 채혈기는 자동화 채혈 장비로써 혈관 판독부터 시작하여 혈액 튜브에 혈액을 담는 과정까지 설계되어있다. 올인원 채혈기를 사용하면 자동화 기구로 채혈하기 때문에 임상병리사가 주삿바늘에 직접 노출되는 경우가 줄어들어 감염을 예방할 수 있고, 채혈기 내에서 정맥을 식별해 정확한 채혈을 진행할 수 있다. 또한, 채혈 시간의 감축으로 인해 환자의 대기시간을 감소시킬 수 있다.

• Korean Journal of Clinical Laboratory Science
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• v.53 no.4
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• pp.326-332
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• 2021

Swabs are useful and common sampling tools in various research fields, such as medicine, ecology, biotechnology, forensic medicine, and pollutant monitoring systems. Collection reagents are one of the essential components in sampling. It is important to develop a sample collection kit and designate an appropriate storage temperature because samples need to be stored for a long time. The purpose of this study was to identify the effects of three collection reagents and three storage temperatures on the recovery of living bacteria without media. We selected Escherichia coli and Staphylococcus aureus as representative environmental bacteria. Distilled water (DW), phosphate buffered saline (PBS), and Tris-EDTA (TE) buffer were used as collection reagents and stored at 22℃, 4℃, and -70℃ after sampling. The results of using each collection reagent and storage temperature on the bacteria were compared using relative light units (RLU) and the number of colony forming units (CFU). When using -70℃ storage temperature and the TE buffer, the number of living bacteria and the RLU values remained constant. It is therefore recommended that the sample be stored at -70℃ immediately after collection and a TE buffer solution be used as the collection reagent.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.477-488
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• 2017

This study presents a discussion on the biomedical laboratory science (formally clinical laboratory science or medical laboratory science) with the identity of biomedical laboratory science, as well as the academic classification system for systematic approach. The field of biomedical laboratory science is not registered in the academic research area classification system of the National Research Foundation of Korea. Since the inception of the first department of biomedical laboratory science in 1963, about 52 departments were since established. Despite the scientific identity, biomedical laboratory science have not been acknowledged professionally in most institutions. Observing the academic research area classification, the physical therapy, occupational therapy, and dental hygiene science are systematically classified and approved the identities by the authorities. This study is freshly academic area classification system of the biomedical laboratory science. The contents of this study are summarized as follows. The medical laboratory technologist's discipline is considered within the medical and science category, clinical pathology in class, and biomedical laboratory science in division. Sections of biomedical laboratory science include hematology, transfusionology, immunology, biochemistry, microbiology, parasitology, science, molecular biology, histology, cytology, cardiopulmonary physiology, and neurophysiology.

• Proceedings of the Korean Society of Computer Information Conference
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• 2021.07a
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• pp.351-354
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• 2021

본 연구에서는 기존 업체들이 활용하고 있는 반려동물 생체인식 기술 기반 통합 서비스 앱을 제안한다. 이 앱은 반려동물 미등록자의 미등록 사유를 바탕으로, 접근성 및 노출 빈도가 높은 스마트폰 앱으로, 등록 방식은 안면, 비문, 홍채, DNA 등록을 활용한다. 하나의 생체인식 방법을 사용하는 것이 아닌 다중 인식 방법을 제공하고, 각 인식 방법별 정확도의 비중을 달리하여 오차를 줄이고, 기존의 등록 방식 및 앱과의 차별화를 시도하고자 한다. 또한, CUPET 앱은 단순 등록에 그치지 않고, 실종 및 유기 동물 찾기, 예방접종 주기 및 반려동물 생애주기 정보 제공, 사용자들의 데이터 및 병원 연계를 통해 반려동물 유형별 병원 추천 등의 서비스를 제공하고자 한다. 본 연구에서 제안하는 CUPET 앱을 통하여, 등록 방식의 간략화로 반려동물 등록률 증가, 개인의 반려동물 인식 장치 소유 가능으로 실종 및 유기 동물에 대한 신속한 보호가 가능할 것으로 사료된다.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.3
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• pp.203-212
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• 2023

Since the coronavirus disease-2019 (COVID-19) outbreak, it has been generally believed that a medical technologists (MTs) are supposed to perform polymerase chain reaction tests and next-generation sequencing (NGS) in the hospitals. However, many do not recognize that the duty of MT for clinical genetic testing has not been stated in the Medical Laws (72.5% for MT, N=200; 62.8% for students, N=123). In this regard, to evaluate the feasibility of MT's role for NGS genetic testing, we requested our subjects to fill out an online survey and analyzed the data. Among them, it shows that the scope of MT's role, including NGS performance should include clinical genetic testing (99.5% for MT, N=200; 86.8% for students, N=123). Also, questions on clinical genetics, which is associated with both cellular genetics and molecular genetic questions should be included in the National MT License Problem Bank (97.5% for MT; 71.4% for students). Based on these results, the Korean Association of Medical Technologists needs to cooperate synergically with the Academic Association of Biomedical Laboratory Science with respect to genetic education and legislation for the future benefit of both MTs and students.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.1
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• pp.71-77
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• 2019

Menadione is known as an anti-tumor factor. Many studies have reported the potential anti-cancer role of menadione against a range of cancer cell lines. In this study, the anti-cancer effects of menadione and the underlying molecular signaling involved in apoptosis was investigated in gastric cancer cell lines. The menadione treatment decreased the cell viability of MKN45 gastric cancer cells. The decreased cell viability was attributed to the induction of apoptosis, which was confirmed by the results indicating the activation of caspase-3 and -7 and the cleavage of PARP in Western blotting. The upstream regulatory molecules involved in apoptosis were investigated further and it was discovered that menadione reduced the expression of survivin, an inhibitor of upstream apoptosis proteins. In addition, a transcription factor ${\beta}$-catenin, which is known to regulate survivin expression, was down-regulated by menadione. A previous report showed that menadione inhibited XIAP expression to induce apoptosis and induced G2/M cell cycle arrest in AGS cells. This study elucidated another inhibitory mechanism of menadione against gastric cancer cells in a different cell line. Although further studies will be needed, the inhibitory mechanism demonstrated in this study will help better understand the anti-cancer effects of menadione.

• Journal of radiological science and technology
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• v.34 no.2
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• pp.131-140
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• 2011

This study is to investigate the satisfaction rate and relative factors from the students majored in Radiology, Clinical Pathology and Physical Therapy, which can provide basic knowledge of satisfaction and guide the university life and further careers. The survey was performed with respect to 107 students of Radiology, 97 of Clinical Pathology and 89 of Physical Therapy at three-year or four-year colleges(universities) located in Busan and Gyeongsangnam-do. The results show that the satisfaction rate through whole majors was $3.40{\pm}0.57$ point. The scores of each major are: $3.38{\pm}0.62$ for Radiology, $3.37{\pm}0.56$ for Clinical Pathology, and $3.45{\pm}0.50$ for Physical Therapy. The score of social status was highest and the environment of major was the lowest. The satisfaction of three-year colleges was higher than that of four-year universities. Academic system, social status and adaptation to major affected satisfaction on major. In addition job outlook affected on all majors. Thus, we propose that every major must be equipped with specialized environment different from general colleges (universities), and provide environment where students can cause self-motivation. In addition, we found the special way to improve social status and satisfaction by using career guidance, offering career information and performing university-industry collaborations. Especially, it is important to improve the satisfaction on major of students at four-year universities.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.2
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• pp.71-81
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• 2023

This study examined the transition process of clinical laboratory technologists' national examination and job definition in Korea and compared the differences in the national examination between Korea and neighboring countries, such as Japan and Taiwan. In Korea, the number of questions made for it was 200 (1965), 200 (1977), 300 (1982), 250 (1992), 330 (2006), and 280 (2015). The practice of clinical physiology is important for real-time monitoring, given the characteristics of physiological testing. On the other hand, there are conflicts between other occupations in the working area. Clinical molecular biology needs to be established as a new major subject considering the diagnostic importance of molecular biological tests and the speed of science and technology development. Clinical laboratory operations provide policy and guidance recommendations to technologist staff. The proposed clinical laboratory technologists' national examination comprises major subjects: clinical biochemistry, clinical hematology, clinical transfusionology, clinical immunology, clinical microbiology, clinical molecular biology, clinical histology, clinical cytology, clinical physiology, and clinical laboratory operations. In addition, this study proposes the job definition of clinical laboratory technologists, revising various chemical or physiological testing to biomedical or physiological testing required for medical practice.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.3
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• pp.153-158
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• 2015

In order to find out a method to improve awareness of biomedical laboratory science about forensic medicine education and suggest a plan for invigoration, we carried out a survey targeting 100 students of biomedical laboratory science of Daegu Health College in Daegu region and implemented their awareness and requirement forensic medicine subject, which suggested very high awareness of most of the students about forensic medicine as a result. Out of 100 respondents, in the result of survey for students' awareness of forensic medicine, 94 (94%) answered that they were aware of it, We could find that they have high expectation from forensic medicine, and current biomedical laboratory science showed very high awareness and requirement for forensic medicine education. Therefore, rather than focusing only on core courses required for medical technologist, it is necessary to set up a program to introduce forensic subjects for expansion of professional areas and activation which can promote employment of medical technologists.