• Title/Summary/Keyword: 인지실험

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Two Phenolic Compounds Isolated from Umbilicaria esculenta as Phospholipase $A_2$ Inhibitors (석이로부터 분리한 페놀성 화합물의 phospholipase $A_2$ 저해활성)

  • Kim, Jin-Woo;Song, Kyung-Sik;Yoo, Ick-Dong;Chang, Hyeun-Wook;Yu, Seung-Hun;Bae, Kang-Gyu;Min, Tae-Jin
    • The Korean Journal of Mycology
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    • v.24 no.3 s.78
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    • pp.237-242
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    • 1996
  • Two depsides, medicinal herb products isolated from the methanol extract of Umbilicaria esculenta, inhibited human synovial fluid Phospholipase $A_2\;(PLA_2)$ ($IC_{50}$ of 0.22 and 0.26 mM, respectively). In the course of screening for antiinflammatory compounds from natural products, we successfully isolated two depsides $PLA_2$ inhibitory compounds, Orcinol and methyl orsellinic acid. The compounds were identified as orcinol and methyl orsellinic acid on the basis of various NMR studies including $^1H,\;^{13}C$ and DEPT experiments.

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Pretreatment with GPR88 Agonist Attenuates Postischemic Brain Injury in a Stroke Mouse Model (GPR88 효현제의 전처리에 의한 뇌졸중후 뇌손상 감소효과 연구)

  • Lee, Seo-Yeon;Park, Jung Hwa;Kim, Min Jae;Choi, Byung Tae;Shin, Hwa Kyoung
    • Journal of Life Science
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    • v.30 no.11
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    • pp.939-946
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    • 2020
  • Stroke is one of the leading causes of neurological disability worldwide and stroke patients exhibit a range of motor, cognitive, and psychiatric impairments. GPR88 is an orphan G protein-coupled receptor (GPCR) that is highly expressed in striatal medium spiny neurons; its deletion results in poor motor coordination and motor learning. There are currently no studies on the involvement of GPR88 in stroke or in post-stroke brain function recovery. In this study, we found a decrease in GPR88 protein and mRNA expression levels in an ischemic mouse model using Western blot and real-time PCR, respectively. In addition, we observed that, among the three types of cells derived from the brain (brain microvascular endothelial cells, BV2 microglial cells, and HT22 hippocampal neuronal cells), the expression of GPR88 was highest in HT22 neuronal cells, and that GPR88 expression was downregulated in HT22 cells under oxygen-glucose deprivation (OGD) conditions. Moreover, pretreatment with RTI- 13951-33 (10 mg/kg), a brain-penetrant GPR88 agonist, ameliorated brain injury following ischemia, as evidenced by improvements in infarct volume, vestibular-motor function, and neurological score. Collectively, our results suggest that GPR88 could be a potential drug target for the treatment of central nervous system (CNS) diseases, including ischemic stroke.

밀배아 분말첨가가 식빵의 품질특성에 미치는 영향

  • 김래영;전순실;서재수
    • Proceedings of the Korean Journal of Food and Nutrition Conference
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    • 2000.12a
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    • pp.51-51
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    • 2000
  • 식생활의 서구화로 제빵 산업이 발달하고, 빵을 주식으로 하는 인구가 늘고 있다 또한 기존의 재료보다는 기능성이 첨가된 부재료를 활용한 건강 지향적인 식품의 수요가 증가하고 있는 추세이다. 최근 밀배아의 용도 다양화를 위하여 팔은 연구가 진행되고 있으며, 상업적으로 이용되고 있다. 소맥의 제분과정에서 부산물로 얻어지는 밀배에는 식물 중에서 가장 많은 천연 비타민 E 복합체가 이상적인 상태로 함유되어 있다. 밀배아에 들어 있는 비타민 E는 천연 비타민 E 복합체와 합성 비타민 E로 구분되어진다. 천연비타민E는 8가지 이성체를 가진 자연 비타민 E를 말하며, 4종류의($\alpha$$\beta$${\gamma}$$\delta$)의 토코페롤과 4종의 토코페롤의 복합체이다. 비타민 E는 사람을 포함한 고등동물에 필수적인 영양소로 식물성기름, 곡류, 배아 등에 많이 들어 있으며, 여러 이성질체중 $\alpha$-토코페롤이 체내에서 가장 생물학적 활성이 큰 것으로 알려졌으며, 생체막조직에서 인지질의 천연항산화제 역할을 하여 체내대사를 정상으로 유지하는데 큰 기능을 하는 것으로 보고되고 있다. 우리나라에서 식용으로 소비되는 밀은 거의가 수입에 의존하고 있으며, 밀배아는 거의 전량이 가축의 사료용으로 사용되고 있고 근래에 와서는 배아유, 배아떡, 복합 조미료, 쿠키, 배아를 첨가한 국수 등에 이용되고 있다. 따라서 본 실험에서는 식빵제조시 밀배아 분말을 0, 2, 4, 6, 8, 10%씩 첨가하여 기능성 식빵을 제조하였을 때의 반죽의 물성, 호화특성을 살펴보고 빵으로 제조하였을 때의 식빵의 품질 특성으로서 1차 발효 손실률, 굽기 손실률, 부피, 질감, 노화도, 관능적 특성 및 mould-free shelf life를 측정하였다.te, ferric citrate가 유제품에 사용하기에 적합한 것으로 나타났다. 생이용성을 평가하기 위하여 우유에서 low molecular weight components(ILC)를 분리하고 철분과 복합체를 형성시킨 다음, 철분 결핍된 쥐의 소장에서 loop을 형성시켜 ILC-철분 복합체를 injection하여 철분 흡수도를 조사하였다. Ferrous lactate 100ppm에서 약 25.6%흡수되었고 ferric citrate 100ppm은 24.7%, ferrous sulfate는 19.7%흡수되었다. ILC를 첨가하지 않은 100ppm 철분염 용액은 ferrous sulfate를 제외하고는 흡수도가 감소되었다. 철분 결핍된 쥐에게 gavage 방법에 의하여 철분강화우유를 투여하였을 때 철분 25ppm 시료에서는 ferrous sulfate가 12.5%로 가장 높았고 ferrous lactate는 8.1%, ferric citrate는 6.5% 흡수되었다. 철분 100ppm수준에서는 흡수율이 낮아져 ferrous sulfate는 25ppm 시료보다 절반이하 수준이었다. Ferric citrate는 차이가 거의 없었으며 ferrous lactate는 70%수준이었다. 이상의 결과에서 철분강화우유에 사용하기 적합한 철분염은 ferrous lactate, ferric citrate였는데 특히 ferrous lactate는 제품의 이화학적 품질, 생이용성 측면 모두에서 가장 좋은 것으로 나타났다.다 높았으며, 1회당 평균 8.1$\pm$5.1개의 난포란을 회수하였다. investigation can be separated into sampling and analytical uncertainties, it can be used as a criterion where the resources for the inves

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Effects of the Feeding Mixed Oils of the Butter, Sardine and Safflower Oils on Fatty Acid Metabolism of Serum and Liver in Rats (버터, 정어리유 및 홍화유를 혼합 급이한 흰쥐의 혈청 및 간장의 지방산 대사에 미치는 영향)

  • Kim, Han-Soo;Kim, Sung-Hee;Chung, Seung-Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.6
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    • pp.617-626
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    • 1992
  • This study was designed to observe the effects of the feeding mixed oils of the sardine oil containing n-3 EPA(eicosapentaenoic acid) and DHA (docosahexaenoic acid) and the safflower oil in which were plenty of n-6 linoleic acid, on the fatty acid metabolism of serum and liver in the dietary hyperlipidemic rats. Experimental oils mixed with 16% butter(control group), 8% butter + 8% olive oil and 8% butter with various level of sardine and safflower oils were administered to the male rats of the Sprague Dawley for 4 weeks. In the fatty acid compositions of serum phospholipid, triglyceride and cholesteryl ester, according as the contents of mixed safflower oil increased, n-3 PUFA(polyunsaturated fatty acid) contents and ratio of EPA/AA(arachidonic acid) tended to be decreased, but n-6 PUFA contents and ratio of AA/PUFA tended to be increased. In the fatty acid composition of triglyceride component in liver lipid, DHA contents were most in the liver lipid. And components of cholesteryl ester, n-6 linoleic acid contents were high percentage. Fatty acid compositions of serum and liver lipids were influenced from the fatty acid composition of the test lipids.

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Research Trends of Music for People with Visual Impairments: A Review of Journals Published Domestically and Internationally from 1998 to 2017 (시각장애인을 대상으로 한 음악 연구의 동향: 1998-2017년 국내·국제 학술지 논문 비교)

  • Park, Hye Young;Cho, Mi Ran;Yoon, Eun Jeong
    • The Journal of the Korea Contents Association
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    • v.18 no.6
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    • pp.243-256
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    • 2018
  • The purpose of this study was to perform a content analysis on 62 domestic and international journals from 1998 to 2017, in order to review research trends in music and people with visual impairments. The framework of the content analysis was based on the year of publication, field of journal, research method, type of research, research subject, participants' age, and factors of impairment. The results showed that both domestic and international journals have rapidly increased in the last 10 years, and have expanded into the fields of education and therapy. In the research method, survey research was conducted much more frequently in domestic journals, while both qualitative and experimental research were employed on a roughly even basis in international journals. With respect to the type and subject of research, music perception and recent trends on the subject of musical interest and aptitude took up the majority of domestic journals, while international journals focused on music intervention, music perception and cognition with musical influences and experiences. The participants in both domestic and international journals were children, adolescents and adults, while the elderly were not observed. In the factors of impairment, research on people with total blindness or low vision were conducted frequently, but those with adventitious vision loss or multiple impairments were rarely performed. This study could be meaningful to suggest the direction of further studies, through the investigation of research trends in music and people with visual impairments.

천연 항균복합제재에 의한 농산물 식품원료의 선도유지효과

  • 정준호;조성환
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.10a
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    • pp.189.2-190
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    • 2003
  • 현재까지 미생물의 증식을 억제하는 보존제로는 인공합성품이 많이 알려져 이들이 주로 상업적으로 사용되고 있으나 그 안정성이 경우에 따라 문제되고 있으며 근래소비자의 건강 지향적 욕구가 증대됨에 따라 인공합성품의 기피현상이 두드러지고 있다. 따라서 이들 천연 항균물질의 개발과 이용은 인공 합성보존제의 대처라는 의미와 소비자기피현상을 유발시키지 않으면서 각종 가공식품의 저장성향상 및 저온 유통식품의 안정성확보라는 견지에서 그 중요성이 있다. 본 실험에서는 식품의 미생물 작용에 의한 변질의 저해 효과가 뛰어난 식물성천연항균제품(Botanical antimicrobial agents-Citrus product : 이하 BAAC라 칭함)에 천연보조제(Ginseng extract, Aloe, 매실추출물)를 첨가하여 Paper disk 법에 의한 항균성 검사를 실시한 결과 Ginseng extract를 첨가한 경우 생육저해환이 가장 크고 뚜렷하게 나타났다. 따라서 BAAC에 보조제(Ginseng extract)를 첨가하여 제조 된 천연 항균복합제재(이하 BAAG라 칭함)를 우유에는 각 농도별로 첨가하여 4$^{\circ}C$의 냉장상태와 2$0^{\circ}C$의 상온에서 저장하였고, 계란과 콩나물의 경우는 침지 처리한 후 꺼내어 상온에서 저장하면서 각각 주사전자현미경 관찰, 대장균수, 총균수의 변화, 외관상의 변화에 미치는 영향을 무처리 대조구와 비교하면서 조사하였다. 아울러, 계란의 경우는 조단백함량의 변화도 관찰하였다. 주사전자현미경 촬영사진의 결과 분석을 통하여 BAAG를 처리한 균체 세포는 세포막 및 세포벽 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되며 성장을 저해 또는 사멸시키는 것으로 나타났다. 대장균수는 우유, 달걀 및 콩나물의 모든 처리구에서 BAAG의 첨가에 의해 성장이 억제되었으며 농도가 증가시킬수록 균증식 억제 효과가 뚜렷하였으며, 총균수도 압도적으로 낮은 간을 보여주었다. 달걀의 조단백함량의 변화는 BAAG를 처리하지 않은 무처리 대조구에 비하여 침지 처리한 경우가 조단백 변화가 크지 않았다. 즉, BAAG의 침지 처리한 경우, 저장기간 14일이 경과 한 후에 10%정도의 수준으로 감소한데 반하여, 무처리구인 대조구의 경우, 23%수준으로 감소 증도가 증가하였다. 외관상의 변화와 상품 가치를 측정한 결과는 달걀의 경우, 14일이 경과후에는 무처리 대조구에서 흰자위의 감소와 노른자위와의 경계가 뚜렷하지 못하여 상품으로서의 가치가 크게 떨어진 상태였다. 우유의 경우에는 4$^{\circ}C$와 2$0^{\circ}C$에 저장된 대조구에서는 각각 3일과 12일경과 후 강한 부패취와 아울러, 표피의 갈변정도가 심하되어 관능적으로 부패상태를 인지할 수 있었다. 콩나물의 경우도 저장3일 경과 후에 부패취와 함께 점질성 갈변물질이 생성되었다. 이와 같은 결과로 볼 때, BAAG의 처리는 BAAC의 경우보다 가격은 저렴하면서도 항균력은 우수한 천연 항균복합제재로써 농산물 식품원료에 적용하여 선도유지 기간을 연장할 수 있는 효과를 기대할 수 있었다.

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The System of Converting Muscular Sense into both Color and Sound based on the Synesthetic Perception (공감각인지 기반 근감각신호에서 색·음으로의 변환 시스템)

  • Bae, Myung-Jin;Kim, Sung-Ill
    • Journal of the Korean Institute of Intelligent Systems
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    • v.24 no.5
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    • pp.462-469
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    • 2014
  • As a basic study on both engineering applications and representation methods of synesthesia, this paper aims at building basic system which converts a muscular sense into both visual and auditory elements. As for the building method, data of the muscular sense can be acquired through roll and pitch signals which are calculated from both three-axis acceleration sensor and the two-axis gyro sensor. The roll and pitch signals are then converted into both visual and auditory information as outputs. The roll signals are converted into both intensity elements of the HSI color model and octaves as one of auditory elements. In addition, the pitch signals are converted into both hue elements of the HSI color model and scales as another one of auditory elements. Each of the extracted elements of the HSI color model is converted into each of the three elements of the RGB color model respectively, so that the real-time output color signals can be obtained. Octaves and scales are also converted and synthesized into MIDI signals, so that the real-time sound signals can be obtained as anther one of output signals. In experiments, the results revealed that normal color and sound output signals were successfully obtained from roll and pitch values that represent muscular senses or physical movements, depending on the conversion relationship based on the similarity between color and sound.

Fast information extraction algorithm for object-based MPEG-4 conversion from MPEG-1,2 (MPEG-1,2로부터 객체 기반 MPEG-4 변환을 위한 고속 정보 추출 알고리즘)

  • 양종호;박성욱
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.41 no.3
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    • pp.91-102
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    • 2004
  • In this paper, a fast information extraction algorithm for object-based MPEG-4 application from MPEG-1,2 is proposed. For object-based MPEG-4 conversion, we need to extract such information as object-image, shape-image, macro-block motion vector, and header information from MPEG-1,2 bit-stream. If we use the extracted information, fast conversion for object-based MPEG-4 is possible. The proposed object extraction algerian has two important steps, namely the motion vector extraction from MPEG-1,2 bit-stream and the watershed algerian The algorithm extracts objects using user's assistance in the intra frame and tracks then in the following inter frames. If we have an unsatisfactory result for a fast moving object the user can intervene to connect the segmentation. The proposed algorithm consist of two steps, which are intra frame object extracting processing and inter frame tracking processing. Object extracting process is the step in which user extracts a semantic object directly by using the block classification and watersheds. Object tracking process is the step of the following the object in the subsequent frames. It is based on the boundary fitting method using motion vector, object-mask and modified watersheds. Experimental results show that the proposed method can achieve a fast conversion from the MPEG-1,2 bit-stream to the object-based MPEG-4 input.

Recognition of Superimposed Patterns with Selective Attention based on SVM (SVM기반의 선택적 주의집중을 이용한 중첩 패턴 인식)

  • Bae, Kyu-Chan;Park, Hyung-Min;Oh, Sang-Hoon;Choi, Youg-Sun;Lee, Soo-Young
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.42 no.5 s.305
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    • pp.123-136
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    • 2005
  • We propose a recognition system for superimposed patterns based on selective attention model and SVM which produces better performance than artificial neural network. The proposed selective attention model includes attention layer prior to SVM which affects SVM's input parameters. It also behaves as selective filter. The philosophy behind selective attention model is to find the stopping criteria to stop training and also defines the confidence measure of the selective attention's outcome. Support vector represents the other surrounding sample vectors. The support vector closest to the initial input vector in consideration is chosen. Minimal euclidean distance between the modified input vector based on selective attention and the chosen support vector defines the stopping criteria. It is difficult to define the confidence measure of selective attention if we apply common selective attention model, A new way of doffing the confidence measure can be set under the constraint that each modified input pixel does not cross over the boundary of original input pixel, thus the range of applicable information get increased. This method uses the following information; the Euclidean distance between an input pattern and modified pattern, the output of SVM, the support vector output of hidden neuron that is the closest to the initial input pattern. For the recognition experiment, 45 different combinations of USPS digit data are used. Better recognition performance is seen when selective attention is applied along with SVM than SVM only. Also, the proposed selective attention shows better performance than common selective attention.

A Conspicuity Effect Study of Fluorescent Orange Color Traffic Sings for Work Zone Application (공사구간 형광주황색 교통안전표지 적용에 따른 주목성 효과 연구)

  • Ko, Sangkeun;Choi, Keechoo;Lee, Sang-Soo;Yun, Ilsoo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.5D
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    • pp.437-444
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    • 2012
  • This study intended to improve traffic safety in work zones using fluorescent orange color traffic signs. For this end, the current problems of existing traffic signs and facilities in work zones were analyzed and some good examples of foreign countries were compared. More specifically, in order to identify current problems, the basic shape and color of traffic signs in use were examined through literature review, surveys and field studies. It was found that the fluorescent color worked more effectively than other colors in terms of visibility and conspicuity in work zone sign system. For evaluation, both Conspicuity surveys and in-door simulator experiments were conducted to effectively assess the merits and demerits of different types of traffic signs for work zones. The results of evaluations showed that black lettering on a fluorescent orange background outperformed more than 70% in terms of visibility and ability to call drivers' attention compared with those from black/red lettering on yellow/white backgrounds currently used at work zones on expressways and national highways. In addition, simulated driving experiment disclosed that drivers recognized the fluorescent orange background sign 15m ahead compared with the yellow background sign and 25m ahead compared with the white background sign, respectively. As for the diamond-shaped "Under Construction" sign, drivers recognized fluorescent orange background 11m ahead compared with the yellow background sign and 19m ahead compared with the white background sign, respectively.