• Journal of Life Science
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• v.22 no.9
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• pp.1254-1260
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• 2012

The moringa (Moringa oleifera Lam.) plant is used both as food and an anti-allergic agent. In this study, we investigated skin protection effects of methanol extracts from the root, seed, fruit, and leaves of moringa in HaCaT keratinocyte cells. To investigate the pharmacological potential of various moringa extracts on TNF-${\alpha}$-induced collagen degradation in HaCaT cells, we measured the activity of matrix metallopeptidase-9,2 (MMP-9,2) by zymography analysis. Our results showed that all the moringa extracts inhibit the TNF-${\alpha}$-induced enzyme activity of MMP-9. In particular, moringa root extracts significantly suppressed MMP-9 and MMP-2 in a dose-dependent manner. Next, to investigate the anti-inflammation effect of the moringa extracts, we examined cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and interleukin-6 (IL-6) expression of the extracts. The results showed that both the root extracts and the seed extracts decreased the TNF-${\alpha}$-induced expression of COX-2. In addition, the root and leaf extracts reduced the expression of IL-6. However, none of the moringa extracts affected the expression of iNOS. The results suggest that moringa root extracts down-regulate MMP-9, COX-2, and IL-6 and that the root extracts offer superior skin protection effects compared with other extracts of moringa in HaCaT cells.

• Journal of Life Science
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• v.28 no.8
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• pp.892-899
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• 2018

Portulaca oleracea L. is an edible plant widely consumed in daily diet throughout Europe, Asia and America. In this study, protective effects of P. oleracea L. extracts against oxidative stress and matrix metalloproteinase (MMP) activity induced by ultraviolet B (UVB) radiation were investigated using HaCaT immortal human keratinocytes. In this context, the mRNA and protein productions of MMPs (MMP-1, -2, and -9) and type I procollagen, which are major markers of photoaging induced by UVB radiation in HaCaT keratinocytes, were evaluated. Furthermore, UVB-induced reactive oxygen species (ROS) generation and mRNA and protein expression levels of superoxide dismutase-1 (SOD-1), oxygenase-1 (OH-1), and nuclear factor-erythroid 2-related factor-2 (Nrf-2), all of which are associated with the antioxidant balance, were investigated. As shown by the results, UVB radiation induced ROS formation and led to increased production of MMPs and decreased collagen production in human keratinocytes, which resulted in skin photoaging or photodamage. The treatment with P. oleracea L. extracts downregulated MMP (MMP-1, -2, and -9) production and upregulated type I procollagen expression in UVB-induced HaCaT cells. Furthermore, treatment with the extracts decreased UVB-induced ROS generation and increased the expression of antioxidant enzymes, such as SOD-1 and OH-1, through the Nrf-2 pathway. Taken together, these results suggest that P. oleracea L. extracts could be a potential cosmeceutical agent for the prevention of skin photoaging or photodamage.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.3 s.47
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• pp.389-392
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• 2004

We screened several materials to stimulate IGF-1 promoter activity using luciferase reporter assay and found that Amomi Semen extract (ASE) among them is the most powerful stimulator We also studied about the anti-wrinkle effect of ethanolic extract of Amoni Semen in vitro and in vivo. Semi-quantitative RT-PCR showed that the extract elevated the presence level of IGF-1 mRNA. And $[^3H]$ proline incorporation and semi-quantitative RT-PCR showed that the extract increased the expression of type-I collagen compared with vehicle in vitro and in vivo, respectively. Significant inhibition of MMP-1 expression was determined by ELISA and Western blot. Finally, topical treatment of the extract on hairless mouse's dorsal skin expanded the volume of collagen and dermal thickness. These results suggest that Amomi Semen may be a good candidate for improving extracellular matrix through the increase of collagen expression and inhibition of MMP-1 expression. Moreover, this study enables us to guess that IGF-1 stimulated by the extract may be involved in the mechanism of anti-wrinkle effect of it.

• Journal of Life Science
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• v.31 no.11
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• pp.969-979
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• 2021

Type 2 diabetes is a serious chronic metabolic disease, and the goal of diabetes treatment is to keep blood glucose at a normal level and prevent complications from diabetes. Hyperglycemia is a key pathologic feature of type 2 diabetes that mainly results from insulin resistance and pancreatic β-cell dysfunction. Chronic exposure of β-cells to elevated glucose concentrations induces glucotoxicity. In this study, we examined whether an 80% ethanol extract of Oxya chinensis sinuosa Mishchenko (OEE) protected INS-1 pancreatic β-cells against glucotoxicity-induced apoptosis and oxidative stress. Pretreatment with a high concentration of glucose (high glucose = 30 mM) induced glucotoxicity and apoptosis of INS-1 pancreatic β cells. Treatment with OEE significantly increased cell viability. Treatment with 0.01-0.20 mg/ml OEE dose dependently decreased intracellular reactive oxygen species, lipid peroxidation, and nitric oxide levels and increased insulin secretion in high glucose-pretreated INS-1 β cells. OEE also significantly increased the activities of antioxidant enzymes in response to high-glucose-induced oxidative stress. Moreover, OEE treatment significantly reduced the expressions of pro-apoptotic proteins, including Bax, cytochrome C, caspase-3, and caspase-9, and increased anti-apoptotic Bcl-2 expression. Apoptotic cells were identified using Annexin-V/propidium iodide staining, which revealed that treatment with OEE significantly reduced high-glucose-induced apoptosis. These findings implicate OEE as a valuable functional food in protecting pancreatic β-cells against glucotoxicity-induced apoptosis and oxidative stress.

• Proceedings of the KSRS Conference
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• 2001.03a
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• pp.42-48
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• 2001

오늘날 원격탐사와 GIS를 이용한 시·공간적 분석은 인간활동에서부터 자연환경에 이르기까지 다양한 정보를 추출하기 위한 기법으로 자주 사용되고 있다. 본 연구는 위성원격 탐사자료와 GIS를 활용하여 시기별 도시지역에서의 열 분포 특성을 추출하여 토지피복과의 상관관계를 시·공간적으로 해석하였다. 이를 위하여 세 시기간 도시 열 분포의 특성을 도시성장과 함께 해석함과 동시에 보다 명확 하게 규명하기 위하여 Landsat TM band 6의 DN value를 이용한 지표온도 추출에 있어서 NASA 모델을 활용하여 대구시 주변지역 8개 지점의 AWS 실측 값과 서로 상관 분석한 결 과 평균 0.85의 상관정도를 얻었다. 또한 토지피복분류를 통하여 도시성장에 따른 열 분포 및 식생지수의 변화를 시·공간적으로 해석하기 위하여 1,000지점에서 sample 자료를 추출 하여 지형특성별 열 분포의 패턴을 분석하였다. 이와 같은 결과는 향후 도시환경 특성을 고 려한 환경 친화적인 도시계획수립에 있어서 중요한 인자로 작용할 것으로 사료된다.

• The Journal of the Acoustical Society of Korea
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• v.15 no.6
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• pp.104-109
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• 1996

In passive sonar system, frequency lines generated by underwater target are very important for detection, tracking and classification. In this paper, the extraction method of unstable frequency line from the time samples of the radiated noise of underwater target is studied. As unstable frequency line is time varying, an extended Kalman filter algorithm which is desirable for nonlinear system is applied to extract unstable frequency line. The proposed method shows good extraction of unstable frequency line by application of simulated signal and real target.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 1998.06a
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• pp.22-26
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• 1998

토양오염 정화방법의 하나인 토양증기추출법(soil vapor extraction, SVE)은 오염된 토양에 진공 또는 가압의 공기를 공급하여 연속적인 공기 흐름을 유도함으로써 토양의 기공에 잔류하는 유해화합물의 증발을 촉진하여 오염물질을 제거하는 공정이다. 본 연구에서는 토양증기추출법의 효율에 영향을 주는 인자들 가운데에서 토양의 수분함량과 오염물질의 종류가 오염물질의 제거효율에 미치는 영향에 대한 실험과 계산을 수행하였다. 인공토양으로 glass bead, sand, molecular sieve가 사용되었으며. 오염물질로는 톨루엔. 메틸에틸케톤, 트리클로로에틸렌이 사용되었다. 각 실험에 대하여 프로인들리히 등온식과 기공확산모델 등을 고려하여 계산을 수행한 결과, 수분이 없는 경우에는 탈착식에 의해, 수분이 있는 경우에는 interparticle에서의 확산 현상에 의해 오염물질의 제거속도가 지배됨을 알 수 있었다. 이러한 연구결과는 정화대상지역에 SVE를 이용한 적절한 정화방법을 설계하는데 기초자료로 이용할 수 있을 것이다.

• KSBB Journal
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• v.9 no.4
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• pp.406-411
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• 1994

An extractive fermentation process was developed to produce citric acid from g1ucose. Citric acid is a strong inhibitor to this fermentation. A mixture of tertiary amine and oleyl alcohol was used to selectively extract citric acid from the fermentation broth, hence enhancing the productivity by over 200%. Although the toxicity of the solvent was significant in the range of higher than 30% of amine, immobilization in polyurethane foam was useful to protect the cells from the toxicity of the solvent.

• The Journal of Engineering Geology
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• v.10 no.3
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• pp.263-272
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• 2000

The main objective of this study is to simulate the rainfall-runoff relationship of the Ohwon rivet basin. For the this study, we used GIS technique and HMS(Hydrological Modeling System). In this study, watershed itself and geometric factors of watershed are extracted from DEM by using a GIS technique. The scanned data of topographical map with scale of 1:50,000 in the Ohwon river basin is used to this study and it is converted to DEM data. The parameters of Hydrological Modeling System as watershed area(A), river length, SCS Curve Number(CN) etc. are extracted by using the GIS technique in the Ohwon Basin. Extracted parameters are applied to the Hydrological Model System, then the paramenters optimized by the observed data and rainfall data. Then, the optimized parameters and Hydrological Modeling System are applied to the study area for the simulation of rainfall-runoff relationship. With the resultn of this study, GIS technique is useful to the extraction of watershed characteristics factors and Hydrological Modeling System is successful to the simulation of rainfall-runoff relationship.

• Journal of the Korean Geographical Society
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• v.41 no.3 s.114
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• pp.319-330
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• 2006

The objective of this research is to achieve an objectivity of site decision after extracting site decision factors on a large-scale discount store(LSDS) and utilize any hidden information using the association rules mining through huge database. To catch this objective, we collect a census, economic, and environmental dataset related with locating of LSDS. And then, we construct a spatial data on the research area. These data is used for the extraction of a spatial association rules. To verify whether the extracted rules are suitability or not, we use the sales of some LSDS. As the result of test, the more sales, the more factors of the extracted rules relate with the sales it coincides. Consequently, the spatial association rules mining is efficient method which support the ideal site decision of LSDS.