• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.2
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• pp.195-201
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• 2014

Body fluid has been studied for diverse fields like Ringer's solutions, artificial joint fluids, cell growth culture media because it plays a crucial role in controlling body temperature and acts as a solvent for diverse metabolite processes in the body and delivery media of mineral, energy source, hormone, signal and drug from and to cell via blood or lymphatic vessel by osmotic pressure or active uptake. Stratum corneum containing extracellular lipids and NMF (natural moisturizing factor) absorbs atmospheric water residing outside of cells and utilize it to hydrate inside of their own. This process is related to skin barrier function. In this study, we conducted the cell viability test with Cell Bio Fluid $Sync^{TM}$, which mimicks body fluids including amino acids, peptides, and monosaccharides to strengthen skin barrier, and the clinical skin improvement test with cosmetics containing Cell Bio Fluid $Sync^{TM}$. In the cell viability test, HaCaT cell was treated with PBS for 3 hours, followed by the treatment of a cell culture medium (DMEM) and isotonic solution (PBS) and Cell Bio Fluid $Sync^{TM}$ for 3 hours each. Then, MTT assay and image analysis were conducted. In the clinical skin improvement test, twenty-one healthy women participated. Participants applied cosmetics containing Cell Bio Fluid $Sync^{TM}$ on their face for a week and evaluated the skin hydration, skin roughness, brightness and evenness. All measurements were conducted after they washed off their face and took a rest under the constant temperature ($22{\pm}2^{\circ}C$) and constant humidity conditions ($50{\pm}5%$) for 20 minutes. All the data were analyzed by SPSS (version 21) software program. Results showed that Cell Bio Fluid $Sync^{TM}$ improved both the cell viability and in vivo skin conditions such as skin hydration, roughness, brightness and evenness.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.2
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• pp.127-139
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• 2023

In this study, minoxidil, which is well known as a pharmaceutical raw material, and diaminopyrimidine oxide (DAO), which is a cosmetic raw material, were used as active ingredients to evaluate the physical properties of niosomes and compare the skin penetrations of artificial skin. To prepare niosomes of the size of nanoparticles, a high pressure homogenization method was used, and physical properties were evaluated with a zetasizer. The particle size of the noisome including the active ingredient was measured to be 99 to 123 nm according to HLB, and the zeta potential was measured in the range of -60 to -81 mV. Through DSC (differential scanning colorimetry), it was confirmed that minoxidil, a crystalline component, was uniformly dissolved in an amorphous state in niosomes. In order to confirm and compare skin penetration, it was measured by the in vitro Franz diffusion cell method, and the niosome formulation showed 3.4 times higher penetration for minoxidil and 11.1 times higher penetration for DAO than the control gel formulation. In addition, when comparing the skin penetration of minoxidil niosome and DAO niosome, a similar trend was shown, and the penetration amount of DAO was relatively high. The shapes of the niosome formulations with different HLB values were observed using Cryo-TEM, and it was confirmed that vesicles were formed in all of them and that they were intermediate between SUV (small unilamella vesicle) and LUV (large unilamella vesicle). Through this study, minoxidil, an effective drug for hair loss, and DAO, a cosmetic raw material, can be effectively delivered to the skin by encapsulating them in a noisome formulation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.3-3
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• 2010

소나무에서 추출해낸 천연유기유황(Natural Sulfur)의 의학적 가치는 1972년 Jacob 박사와 Herschler 박사가 오래곤 과학대학에서 천연식이유황(Natural Sulfur/MSM)을 가지고 표피조직에 미치는 영향을 구명하면서 keratin 단백질에 대한 연구가 활성화 되기 시작하였다. 세포내 골격물질은 크게 형태와 조성에 따라서 actin microfilament, microtubule, 그리고 intermediate filament(IF)로 구분된다. keratin의 특성은 keratin intermediate family중에서 K17 IF가 새로운 기능을 나타내는데 피부세포의 성장에 핵심적인 조절 역할을 한다는 사실이 밝혀 지면서 Dr. Pierre A. Coulombe(The Johns Hopkins University School of Medicine)연구실은 브로컬리와 같은 십자화과 식물 등에 과량 존재하는 항산화 및 항암물질인 sulforaphane이 K17의 발현을 특이적으로 증가시킨다는 것을 알아내어 피부박리와 같은 피부손상을 기능적으로 복구시킬수 있음을 확인하였다. 현재는 수포성 표피박리증 환자군의 많은 부분을 차지하는 K14 돌연변이와 동일한 유전적 변형을 일으킨 생쥐모델을 이용한 약물 효과 검증과 전 임상단계의 인체실험을 함께 진행중에 있다. Mark E. Van Dyke 박사(Wake Forest Institute for Regenerative Medicine Medical Center)는 인간의 머리털에서 유래된 keratin으로 외상에 의한 신경 절단이나 압좌(압박손상)는 현재 다른 부위의 신경을 잘라 이식하거나 절단된 신경 양끝을 인공도관(conduit)으로 연결해 신경재생을 유도하는 미세수술을 시행하게 되는데, 신경재생을 유도하는 도관에 keratin을 주입하면 신경이식과 맞먹는 신경재생 성공률을 기대할 수 있다고 하였다. 앞으로는 동물성 keratin뿐만 아니라 식물성 keratin도 함께 연구할 필요가 있다. 동물성 keratin의 농업적 이용은 가금류 깃털의 keratin을 축출하여 친환경 육묘용 용기를 만드는데 있다. 이 용기는 자연조건에서 생분해될 수 있는 특성을 갖고 있다.

• Science of Emotion and Sensibility
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• v.18 no.3
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• pp.93-106
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• 2015

In accordance with escalating demands for advanced technology products, the smart clothing that includes embedded ICT technology have expanded into fields of daily life. As a result of this trend, interest in smart clothing with digitally controllable heating has rapidly grown and the market for smart heated clothing has also expanded. Increasing of prospect in smart heated clothing market, the effectiveness and thermal sensation research of the location on the pad attached is insufficient. This study was conducted to find the optimal location of heated clothing via experimental research on changes in skin temperature and subjective thermal sensation when heating pads were placed on different areas of the body. For this experiment, the subjects consisted of 10 males in their 20's of standard physique. The skin temperature at 11 different areas of the body, rectal temperature, and subjective thermal sensation were taken at different stages (before testing, after a 20 minute rest period, 20 minute treatment period, and after a 40 minute recovery period) in an artificial-climate chamber at $-5^{\circ}C$. As a result, the optimal location for heating pads in smart clothing was estimated and suggested.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.2
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• pp.137-142
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• 2008

Echinacea purpurea, an indian traditional plant medicine has been widely used as herbal remedy for the treatment of disease such as colds or other infections. However, Echinacea purpurea extracts recently have been applied as a cosmetic ingredient for skin care. We artificially cultured Echinacea purpurea by using the bioreactor culture system for this study. We induced callus from Echinacea purpurea and separated adventitious roots, harvested and extracted after cultured in bioreactors. Previously, several studies have been reported on anti-oxidant and immuno-enhancing effects of Echinacea purpurea extract but other efficacies were not well known. In this study, we investigated the whitening, anti-wrinkle and anti-oxidant effects to know applicable value of tissue-cultured Echinacea purpurea adventitious roots extract(TCEPARE) as a cosmetic ingredient. TCEPARE did not show cytotoxicity until a concentration of 2% and showed the anti-oxidative effect in DPPH and NBT tests. Also, the extract decreased tyrosinase expression in a dose-dependent manner and inhibited melanin synthesis in B16 melanoma cells. TCEPARE reduced protein levels of MMP-1, 2 secreted in culture medium or in cell lysates. From these results we suggest that TCEPARE has potential benefits applicable as to cosmetic ingredient for skin care products.

• Applied Chemistry for Engineering
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• v.33 no.2
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• pp.195-201
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• 2022

The main objective of this work is to prepare Rehmannia glutinosa extract (RE) incorporated functional chitosan (CH) based biomaterials and evaluate their physical properties, RE release properties, inhibitory effect of melanogenesis, and antioxidant and elastase inhibitory activities. RE incorporated CH based biomaterials were synthesized by a casting method and UV curing process. The surface and cross sections of prepared biomaterials were characterized by a field emission scanning electron microscope (FE-SEM). The physical properties such as tensile strength and elongation at break were also investigated. To apply the transdermal drug delivery system, RE release properties were examined with pH 4.5, 5.5, and 6.5 buffer solutions and artificial skin test at 36.5 ℃. Results indicated that RE release of RE incorporated biomaterials with/without the addition of plasticizers [glycerol (GL) and citric acid (CA)] at pH 6.5 was about 1.10 times higher than that of at pH 4.5. In addition, results of the artificial skin test verified that RE was released constantly for 6 h. To verify the applicability of the prepared biomaterials, tyrosinase, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and elastase assays were investigated. Results indicated that RE incorporated biomaterials added CA exhibited tyrosinase activation, DPPH radical scavenging activity rate, and elastase activation of 45.12, 89.40, and 59.94%, respectively.

• Journal of the korean veterinary medical association
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• v.47 no.2
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• pp.165-176
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• 2011

구제역은 우제류 동물이 걸리는 급성열성전염병으로, 본 병에 걸린 동물은 발열이나 식욕부진을 지나 병명대로 구강과 혀 점막, 발굽 주위나 유방의 피부에 수포를 형성한다. 이후 이환된 가축은 섭취 장애나 보행장애가 일어나고, 산육능력이나 비유량이 저하됨에 따른 경제동물로서의 가치를 현저하게 떨어지게 한다. 또한 본 병의 전파는 극히 빠르고 동물이나 축산물에 대하여 엄격한 이동제한이나 방역조치가 본 병의 전파나 확대를 막기 위해 실시하기 때문에 본 병에 의해 생기는 경제적 피해는 농가의 지역단위 뿐만 아니라 국가단위에도 미쳐 심각하다. 그 때문에 자유무역을 표방하는 WTO체재하에 있어서도 구제역의 만연방지를 목적으로 한 무역제한은 국제적으로 용인되어져 오고 있고, 세계동물보건기구에 의해 국가나 지역단위의 청정도 구분이 장해져 각국의 법에 반영되어져 있다. 구제역의 병원체로 있는 바이러스는 숙주역이 넓고, 적지 않은 바이러스양에도 감염이 성립, 감염동물체내에서 바이러스가 신속하게 복제, 감염동물로부터 다량의 바이러스가 배설, 접촉전파만이 아닌 공기전파를 일으킨다는 등이 본 병의 전파나 확대에 유리한 복잡한 성성을 가질 경우에 구제역의 방역 및 박멸을 어렵게 하기 때문에 수의학영역에서 가장 경계해야할 전염병의 하나이다. 따라서 본 필자는 최근의 국내 구제역 발생상황속에서 관련자료정리를 하다가 대동물 임상수의사가 알아야 부분과 일본 동물위생연구소 등에서 구제역바이러스 인공감염 및 화학물질(T-1105)투여시험 결과 내역를 정리하여 소개해두고 저 한다.

• Journal of Chest Surgery
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• v.42 no.2
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• pp.256-258
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• 2009

A 63-year-old man who had a permanent pacemaker placed 17 years ago presented to us with local inflammatory signs on his chest. The skin was opened to remove the permanent pacemaker. However, the pacing wire was not easily removed. So, the permanent pacemaker was removed from the chest with leaving the remnant wire inside. However, the local infection recurred because of the remnant wire. Right thoracotomy was performed and cardiopulmonary bypass was started. After total circulatory arrest, the right atrium was opened and the wire was completely removed.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.14-17
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• 1995

An in vitro construct of three dimensional artificial skin equivalent has been engineered using human cervical epithelial cells and human foreskin fibroblasts with a matrix of bovine type I collagen. Two cell lines were established from cervical uteri cancer tissues which have the HPV(human papillomavirus)18 genome. These two cell lines came from the same origin but have slight differencies in growth rate and tumorigenicity. The organotypic raft culturing of epithelial cells were accomplished at air-liquid interface. The differentiation related characteristics were examined by immunohistochemistry using monoclonal antibodies against EGFreceptor, cytokeratin 5/6/18 as proliferation markers and against filaggrin, involucrin, and cytokeratin 10/13 as differentiation marker. We have obtained the stratification and the differentiation in the artificial skin equivalent, and differentiation-related proteins were expressed more in the C3-artificial skin, and proteins of proliferation were expressed more in the C3N-artificial skin, relatively. We found that reconstituted artificial skin have the same characteristics of differentiation proteins of original tissue or cells of human body.

• Proceedings of the KOSOMBE Conference
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• v.1989 no.05
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• pp.7-8
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• 1989

The graft copolymer of chitosan with amino acid, L-lysine was synthesized by heterogeneous copolymerization and was evaluated as an artificial skin. The mechanical properties under dry and wet state, water content, water vapor transmittance rate and biodegradability were measured. The tensile strength and elongation under wet state ranged $0.3-0.5\;kg/mm^2$, 10-13%, respectively. Water vapor transmittance rate ranged $450-500\;g/m^2{\cdot}day$ like that of the normal skin. The weight loss of prepared membrane by protease IV was measured for the degree of biodegradation. The degree of biodegradation was around 15% and after 4 days it was slow. Biocompatibility was evaluated by studying the attachment of human fibroblast on the prepared membrane surface.