• Journal of Sericultural and Entomological Science
• /
• v.15 no.2
• /
• pp.68.4-68
• /
• 1973

1973면 춘 추기 1∼2령 인공사료육 3령이후 상엽육으로 사육한 결과를 전령 상엽육과 비교하면 다음과 같다. (춘기 사육 결과) 1. 인공사료육은 발육과가 불균일 하여 대조육인 상엽육에 비하여 1∼2령기 64시간 3∼5 령기에 22 시간 연장되어 전령기간중 86시간이 연장 되었다. 2. 감잠비율은 인공사료육의 경우 1∼2령 15%로서 상엽육 7.2% 보다 약 2배에 이르렀으며 화용비율은 인공사료육의 경우 79.4% 로서 상엽육보다 9.5% 낮았다. 이 이유로서는 인공사료의 물리성에 인한 유실잠이 많은 것이 1∼2령기 감잠비율과 화용비율에 큰 영향을 미친다고 생각된다. 3. 대소잠 1 만두 수견량은 인공사료육이 17.4kg으로서 상엽육 20.0kg 보다 13% 낮았다. 이의 주원인도 1∼2 령의 유실잠에 있는것 같다. 4. 유실잠에 의한 생산력의 저하를 밝히기 위하여 1∼2령 유실잠을 제외한 대3령기잠 화용비율 및 1만두 수견량으로 환산하면 상엽육 96.1%에 21.6kg 인공사료육 94.4%에 21.2kg으로서 상엽육에 대한 지수로서는 98.2가 된다. 5. 인공사료육의 견층비율은 21.2% 로서 상엽육의 21.6% 보다는 0.4% 낮고 단견중과 견층중에도 유의의 차가 있었다. 6. 생사량비율 및 해서율에는 유의의차가 없었다.

• Korean journal of applied entomology
• /
• v.36 no.4
• /
• pp.317-322
• /
• 1997

Hatched-larvae of the mulberry longicorn beetle, Apriona germari Hope, collected from mulberry fields were reared on artificial diet at 25$^{\circ}$C with 14 h light and 10 h dark to study the larval developmental characteristics. Artificial diet developed for rearing silkworm was used with minor modification adding mulberry branch powder. In case of artificial diet rearing, the head width of larval instar from the I st to the 12th instars was ranged from 0.12 to 0.69cm, and growth rate of each instar was significantly high between the I st and the 2nd instars. In addition, the weight of the 8th instar larvae was increased approximately 176-fold in comparision with that of the 1st instar larvae. Larval duration of each instar took long with larval developmental stages, and that of the 1st to the 9th or the 12th instars was 186.03 or 304.58 days, respectively. The survival rate of larvae was 40.8% by the 8th instar. The pupation rate was approximately 32.4%. Furthermore, although pupation stage was broadly appeared from the 7th to the I lth instars, pupation was majorly observed at the 8th and the 9th instars.

• Journal of Sericultural and Entomological Science
• /
• v.14 no.2
• /
• pp.67-72
• /
• 1972

It was first succeded in rearing the silkworm on the artificial diets in Japan in 1960. Since then, the researches on the artificial diets have been carried out intensively but the artificial diets have not been applied to the silkworm rearing practically till now. There are difficulties in rearing the silkworm on the artificial diets, such as uneven silkworm growth, higher production cost and decay. The results were not satisfactory, but the author hopefully expects that the artificial diet rearing would be possible for maintaining normal silkworm growth throughout improvement of diet composition, rearing techniques and aseptics add to the diets. Especially the author considered that the aseptic rearing will contribute to the research of sericultural science. Within a few years, the artificial diet rearing would be applied to the practical silkworm rearing.

• Journal of Sericultural and Entomological Science
• /
• v.22 no.1
• /
• pp.59-62
• /
• 1980

The effect of dietary white ginseng on growth of silkworm larva was studied with several young and grown silkworm larvae divided into four groups and fed ad basal artificial diet containing different levels of ginseng extract. The four levels of ginseng extract added to basal artificial diet were: 0 (control), 46.6, 139.9 and 279.8 mg per g of dry diet. The results obtained were as follows: 1. The body weight of silkworm larvae fed ad basal artificial diet containing different levels of ginseng extract was increased in 46.6mg ginseng extract level, 139.9mg ginseng extract level and 279.8mg ginseng extract level order. The body weight was greatly increased in 46.6mg ginseng extract level than in control. It is, therefore. considered that a little white ginseng was effective for larval growth in silkworm rearing with artificial diet. 2. The blood sugar content of silkworm larvae fed on an artificial diet containing several levels of ginseng extract was remarkably decreased as compared wilt that of the control silkworm larvae.

• Journal of Sericultural and Entomological Science
• /
• v.49 no.1
• /
• pp.1-7
• /
• 2007

We investigated the selection and sensitivity to environmental mutagen of silkworm reared artificial diet to develop all-year-round operation system using a specific locus mutation of Bombyx mori. In the system, mutagenicity could be detected by the egg colour manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. Among, hi, Backokjam, C5, and N12, varieties of silkworm, AT was higher than other varieties in eclosion rate of female, and C5 and N12 were higher in fertility of male. Bakokjam was higher in eclosion rate of female, rate of moth to lay eggs normally in female and male, no. of eggs layed in female and fertility of female. As above results, Bakokjam was finally selected as the most fitness one among varieties of silkworm reared artificial diet. But the sensitivity to mutagen was lower than other varieties. In the sensitivity to mutagen, AT was the most sensitivity to mutagen in tested varieties of silkworm. To use AT variety in this system, AT was improved major characteristics, eclosion rate, fertility, rate of moth to lay eggs normally, and so on, by crossing of other varieties including Bakokjam.

• Journal of Sericultural and Entomological Science
• /
• v.24 no.2
• /
• pp.73-80
• /
• 1983

1. The varietal difference in amylase activity of the digestive fluid of the 5th instar larvae reared on the artificial diet was investigated, using the parent commercial silkworm varieties of Japanese strain. The amylase activity was large different among silkworm varieties. The activity was strong in Hansaeng-1, Jam 115 and Jam 117, medium in Hansaeng-3, Jam 113, Jam 119, and Jam 201, weak in Jam 107, Jam 121 and Gyeongchu. The amylase of the digestive fluid of ten parent commercial silkworm varieties is possible-(ae) type, compared with ＋(＋$\^$ae/) type of Daizo. 2. To investigate the effect of a-amylase pre-treatment of the artificial diet, larvae were fed with the diet treated by a-amylase during 4th-5th instar periods. The blood sugar content and cocoon qualities were slightly higher in the experimental larvae than those in the control, while showing the slight less body weight, amylase activity and dietary efficient.

• Journal of Sericultural and Entomological Science
• /
• v.27 no.1
• /
• pp.18-23
• /
• 1985

This study was carried out to investigate the effect of the difference of dietary compositions and environmental conditions on the growth and development of silkworm. The results obtained are as follows: 1. The more amount of soybean meal of diet was added, the more duration of the larval period was shortened. But Fifty percent increase of the amount of protein of diet leongthened the duration of larval period. 2. Fifth percent addition of the soybean meal to the diet does not influence the silkworm mortality, Whereas more than that gives rise to the adverse effect on the silkworm mortality. 3. The cocoon quality was significantly affected by the difference of dietary compesition. 4. In the environmental condition the duration of larval period was shortened in the high temperature and the dark condition. 5. The silkworm mortality was increased in the high temperature and the light condition. 6. The cocoon quality was significantly affected in the high temperature and the dark condition.

• Journal of Sericultural and Entomological Science
• /
• v.37 no.1
• /
• pp.27-32
• /
• 1995

Studies were carried out to report breeding procedure and characteristics of Fb, a strain of silkworm with abnormal food habit polyphagous. Selection was continued up to the 14th generation toward higher incidence of cabbage leaves feeding variants. In the autumn of 1992(7th generation), selected strain were subjected to severe selection by feeding them on artificial diet that did not contain mulberry leaf powder. The results were clearly positive and reached to higher than 95% of cabbage feeding in the 14th generation. This strain was first discovered by feeding cabbage leaves, but later they were observed to eat some kind of plants like lettuce, Chinese cabbage, radish, beet, apple, persimmon, pear, etc. As described above the procedure, cabbage feeding trait was clearly heritable. The strain responsible for this traits was named Fb.

• Journal of Sericultural and Entomological Science
• /
• v.23 no.2
• /
• pp.11-36
• /
• 1982

Forty-nine plant species as additives to silkworm artificial diet and 5 species as cellulose sources for artificial diet were screened for their economic values as feed-resources for the silkworm. Feeding response to artificial diet was tested on 82 silkworm strains. The effect of rearing conditions on feeding response and enzyme activities in the silkworm was investigated. The results were summarized as follows. 1. Seven species out of 49, Vigna sinensis ENDL, Ipomoea vatatas Lamarck, Cyperus anuricus Var. Laxus, Alnus japonica Stendel, Trifolium repens L, Prunus serrulata Lindley. Var, Glycine max L increased feeding response, compared with the basic formula of artificial diet. 2. The economic values of Vigna sinensis ENDL, Ipomoea vatatas Lamarck, Cyperus anuricus Var. Laxus, Ainus japonica Stendel, Cassia tera L, Erigeron canedensis L as feed-resources for artificiale diet were recognized, through feeding experiment during the entire larval stage. 3. Mulberry cellulose showed the best results in rearing and cocoon characteristics. 4. The extent of feeding response varied according to strains and varieties. Varieties in japanese strains showed higher feeding response than those in chinese and european varieties, with considerable variations among a varieties in strains. 5. The begining of 4th instar seems to be a proper time to convert from mulberry to artificial diet, or artificial diet to mulberry, however the middle of 3rd instar seems acceptable. 6. The optimum temperature for artificial diet rearing is 30$^{\circ}C$ during the period of 1st-3rd instar and 28$^{\circ}C$ for 4th-5th instar. 7. Electrophoretic isozyme patterns of esterase and acid phosphatase on agarose gel, as affected by strain. rearing temperature and feed-resources, were observed as follow. (1) Isozyme patterns of mid-gut esterase varied, depending on instar. One or two more isozyme bands were observed in the larvae than feed on the mulberry fed for the artificial diet. (2) A strain, chinese-15 with a higher feeding response, had 1∼2 more bands than chinese-60 with a lower feeding response. (3) Five bands of mid-gut esterase in 3rd and 4th instar larvae reared at 28$^{\circ}C$. and 4 for 3rd instar and 6∼7 for 4th instar larvae at 35$^{\circ}C$ were observed. (4) No similar esterase bands could be found among mid-gut, blood and silkgland. There are five esterase bands in the midgut, one in blood and three in silkgland. (5) There was rather small digerence in acid phosphatase types of mid-gut and blood according to varieties and rearing temperature. No active band was shown in silkgland. In midgut, there was one acid phosphatase band at 3rd instar, two at 4th instar and three at 5th instar. In blood, one active band at 3rd or 4th instar and three bands at 5th inster wire detected.

• Journal of Sericultural and Entomological Science
• /
• v.16 no.2
• /
• pp.35-53
• /
• 1974

A series of experiment was conducted to investigate the effects of the level of dietary Protein (soybean meal) and carbohydrate (sucrose) on the growth rate, feed efficiency, nutrients digestibility and amylase activity of larvae at 5th instar. The results obtained from the experimentation are summarized as follow： (1) It was found that the body weight gain, cocoon quality and feed efficiency of the 5th instar larvae were significantly affected by the level of protein and carbohydrate in the artificial diet. Present data revealed that the proper level of dietary protein and carbohydrate would be the most important factor for the optimum growth of larvae. (2) In the amount of diet consumption, the group of larvae gained more body weight consumed more diet than the group gained less amount of body weight. (3) Digestibility of nutrients was improved as the level of protein in the diet increased, although the level of dietary carbohydrate did not exert any positive results. (4) It was observed that the amylase activity in digestive juice was stimulated by the increasement of dietary carbohydrate. However, no correlation between the level of dietary protein and carbohydrate was detected in the amylase activity. (5) Analytical data indicated that the amount of protein or carbohydrate excreted through feces and urine was proportional to the content of protein or carbohydrate in artificial diet. The group o( larvae consumed the diet contained high protein excreted more protein and less nitrogen free extract than did the group received low protein diet. Likewise, the group of larvae fed high dietary carbohydrate excreted more nitrogen free extract and less protein through excreta than the group consumed low carbohydrate diet group. (6) Although the amylase activity of the larvae received the artificial diet was higher than the larvae received natural diet (mulberry leaf), the rate of body weight gam and the quality of cocoon produced from the natural diet group was better than the artificial diet group. It is, thus, concluded that feeding the larvae by the artificial diet may net be recommendable. (7) It was determined that the best level of protein (soybean meal) and carbohydrate (sucrose) in artificial diet was found to be 40g and 0g, respectively when it was fed to the 5th instar larvae. (8) It may be concluded from the results obtained that the artificial diet that could stimulate the activity of amylase nay not be recommendable for the practical larvae feeding Purpose due simply to the Poor economic return from this diet than natural diet.