• Title/Summary/Keyword: 이준구

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Association of genetic polymorphism in fatty acid synthase with BodyFat and fatty acid composition in Hanwoo (한우 FASN 유전자변이와 한우 지방형질 및 지방산조성과의 연관성 분석)

  • Lee, Seung-Hwan;Kim, Hyeong-Cheol;Cho, Won-Mo;Chang, Sun-Sik;Kim, Bum-Soo;Chang, Gul-Won;Lee, Jun-Hun;Yeon, Seong-Heum;Hong, Seong-Koo
    • Korean Journal of Agricultural Science
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    • v.38 no.2
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    • pp.257-262
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    • 2011
  • The aim of this study was to identify the polymorphism on fatty acid synthase (FASN) gene and its association with fat traits and fatty acid composition in Hanwoo. We genotyped a SNP (g.16024G${\rightarrow}$A) detected in Exon34 of FASN on 90 Hanwoo steers by PCR RFLP. A linear mixed model revealed an association of g.16024G${\rightarrow}$A with total body fat contents (P=0.006), while there is no significant effect between g.16024G${\rightarrow}$A and other traits (IMF, BF and fatty acid composition). Regression coefficient and $R^2$ of the SNP was -1.5 kg and 0.36 in this anslysis. Expecially, AA type of g.16024G${\rightarrow}$A bas a less amount of body fat (1.5 kg) than GG type of the SNP in Hanwoo. In conclusion, this study indicates an important role for FASN gene in determining body fat content in Hanwoo.

Evaluation of Fatigue Damage of Metal Matrix Composite by LFB Acoustic Microscopy (Line-Focus-Beam 초음파 현미경을 이용한 금속복합재료의 피로손상에 관한 연구)

  • Lee, Joon-Hyun
    • Journal of the Korean Society for Nondestructive Testing
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    • v.13 no.2
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    • pp.40-47
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    • 1993
  • Composites composed of a precipitation harden 2124 alloy matrix reinforced by SiC whiskers, which are fabricated by powder metallugy, are suscepttible to fatigue damage due to the pile-up of moving dislocation and the microcrack initiation along SiC-Al interfaces, especially at the external surfaces of a body. The initial process, such as pile-up of dislocation or microcrack, that corresponds to the stage I during fatigue failure process are too small to be detected and characterized by conventional ultrasonic technique. This paper describes the applicability of an acoustic microscope with Line-Focus-Beam(LFB) lens of 225MHz to evaluate fatigue damage of SiC whiskers reinforced Al alloy. The specimens which were 6.6mm thick, 13mm wide, and 105mm long in the gage section were fatigued in tension-tension under load control. The velocity of leaky surface and leaky pseudosurface acoustic waves are obtained by FFT analysis technique from V(z) curve which is a record of output of piezoelectric transducer. These results are discussed with the change of number of fatigue cycles. The result obtained by acoustic microscope is compared with that by ultrasonic technique generated at 5MHz with conventional surface wave transducers.

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Evaluation of suitability and stability in a skeletal Class III complete denture patient with flabby tissue: A case report (Flabby tissue를 동반하는 골격성 Class III 환자의 양악 총의치 수복 및 적합성/안정성 평가 증례)

  • Lee, Junsuk;Hong, Seoungjin;Paek, Janghyun;Noh, Kwantae;Pae, Ahran;Kim, Hyeong-Seob;Kwon, Kung-Rock
    • The Journal of Korean Academy of Prosthodontics
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    • v.56 no.4
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    • pp.295-301
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    • 2018
  • To obtain denture retention, support, and stability in Class III edentulous cases with flat alveolar ridges and extensive flabby tissue is very difficult. The patient was a 72-year-old male who wore ill-fitting 20 year old dentures made by non-medical institutions. There was flabby tissue on the maxillary anterior ridge. The patient showed Angle Class III skeletal relationship with severe protruded mandible. First, temporary dentures were fabricated to restore the masticatory function, and final dentures were made through non- pressure impression technique and careful the arrangement of the posterior resin teeth. Improvement of the retention and stability of the denture during the occlusal force application is reported.

Pilot-scale Production of the Antifreeze Protein from Antarctic Bacterium Flavobacterium frigoris PS1 by Recombinant Escherichia coli with a Cold Shock Induction System (저온 유도 시스템을 가진 재조합 대장균을 이용한 남극 세균 Flavobacterium frigoris PS1 유래 결빙방지단백질의 Pilot-scale 생산)

  • Kim, Eun Jae;Lee, Jun Hyuck;Lee, Sung Gu;Han, Se Jong
    • KSBB Journal
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    • v.30 no.6
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    • pp.345-349
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    • 2015
  • Antifreeze proteins (AFP) inhibit growth and recrystallization of ice, and permit organisms to survive in cold environments. The AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris icebinding protein), was produced in E. coli using a cold shock induction system. The culture temperature was shifted from $37^{\circ}C$ to $15^{\circ}C$ and a 20 L culture scale was used. The final weights of dried cell and FfIBP were estimated to be 126 g and 8.4 g, respectively. The thermal hysteresis (TH) activity ($1.53^{\circ}C$) of the produced FfIBP was 3.6-fold higher than that of the LeIBP (Leucosporidium ice-binding protein) produced in Picha. The current study demonstrates that large-scale production of FfIBP was successful and the result could be extended to further application studies using recombinant AFPs.

Spider Fauna of Mt. Gajisan in Gyeongsangnam-do, Korea (가지산의 거미상)

  • Lee, Sue-Yeon;Kim, Seung-Tae;Jung, Jong-Kook;Yoo, Jeong-Seon;Lee, Joon-Ho
    • Korean Journal of Environmental Biology
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    • v.28 no.2
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    • pp.108-113
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    • 2010
  • Spider fauna of Mt. Gajisan in Miryang, Gyeongsangnam-do was investigated from June to October. Collecting spiders was made by sweeping, beating, sieving and pitfall trapping. Total 66 species of 59 genera belonging to 20 families were identified from 317 collected spiders. Species richness was high in Salticidae (12 species, 20.0%), Theridiidae (8 species, 12.1%), Linyphiidae (5 species, 7.6%), Araneidae, Corinnidae, Gnaphosiade and Thomisidae (4 species, 6.1% repectively), Tetragnathidae, Amaurobiidae and Philodromidae (3 species, 4.5% repectively). Zoogeographical species were 1 Pantropical species (1.5%), 4 Palearctic species (6.1%) and 3 Korean endemic species (4.5%). This result will be a valuable monitoring data in comparative study of mountain spiders.

Releasing of asbestos fibers from the weathered asbestos cement slate roofing (슬레이트 지붕 노후화에 따른 석면 섬유 방출량)

  • Kim, Hyun-Wook;Park, Gye-Young;Han, Jin-Gu;Han, Young-Sun;Hwang, Bum-Gu;Lee, Jun-Hyuk
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.20 no.2
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    • pp.88-93
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    • 2010
  • To confirm and quantify asbestos fibers released from the asbestos-cement slate roofs due to weathering, three houses, selected based on the year of built - 60's, 70, and 80's, were investigated. All of them were located in the downtown of Seoul. Rain or snow-melt water was collected from the roof in a 3.5 liter plastic bottle. A known amount of collected water was filtered on the 37 mm membrane filter, ashed in a muffle furnace, and subsequently treated with HCl to remove organic material. The treated remaining was refiltered on a 25mm membrane filter for PLM and PCM analyses. The NIOSH 7400 method was utilized for PCM counting. In addition, SEM/EDX was used to confirm the asbestos types. The results of this study showed that chrysotile fibers were confirmed by PLM in all samples analyzed. A significant amount of asbestos fibers were found in the water samples. The ranges of asbestos fibers counted from the samples collected in the 60's, 70's, and 80's were; 10,406.3~55,575.6 f/L, 5,218.8~38,126.2 f/L, and 2,906.3~7,798.6 f/L, respectively. As anticipated, concentrations of asbestos fibers increased with time of installment of the roofing material. We conclude that weathering can be a significant factor on the release of asbestos fibers from the asbestos cement products. Since asbestos fibers released into environment can be a source of significant health hazard, countermeasures, such as replacement, removal, and encapsulation of weathered asbestos slate, should be initiated immediately.

Bread Properties Utilizing Extracts of Ganoderma lucidum (GL) (영지버섯 추출물을 이용한 제빵의 특성)

  • 정현채;이준탁;권오진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.7
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    • pp.1201-1205
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    • 2004
  • The purpose of this study was to develop functional breads added with Ganoderma lucidum (GL) extract which was well known for preventing various adult diseases and cancer. It was observed that bitter taste was increased with up to 8% GL extract while dough volume was increased at the first dough fermentation period and baking loss reduction was decreased. In terms of sensory evaluation, there were not so much significant differences with 2% added GL extract. In 4, 6, 8% added GL extract bread, it was found that 4% was ordinary and 6% and 8% were bad compared to the control bread. The bread with 2% added GL extract was excellent, 4% was ordinary and 6, 8% was badly evaluated in the internal texture. It's possible to make the functional breads baking with 2% GL extract which is similar to the control breads based on the above results.

Recent Advances in Structural Studies of Antifreeze Proteins (구조 생물학을 이용한 Antifreeze protein의 최근 연구동향)

  • Lee, Jun-Hyuck;Lee, Sung-Gu;Kim, Hak-Jun
    • Ocean and Polar Research
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    • v.33 no.2
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    • pp.159-169
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    • 2011
  • Antifreeze proteins (AFPs) have ice binding affinity, depress freezing temperature and inhibit ice recystallization which protect cellular membranes in polar organisms. Recent structural studies of antifreeze proteins have significantly expanded our understanding of the structure-function relationship and ice crystal growth inhibition. Although AFPs (Type I-IV AFP from fish, insect AFP and Plant AFP) have completely different fold and no sequence homology, they share a common feature of their surface area for ice binding property. The conserved ice-binding sites are relatively flat and hydrophobic. For example, Type I AFP has an amphipathic, single ${\alpha}$-helix and has regularly spaced Thr-Ala residues which make direct interaction with oxygen atoms of ice crystals. Unlike Type I AFP, Type II and III AFP are compact globular proteins that contain a flat ice-binding patch on the surface. Type II and Type III AFP show a remarkable structural similarity with the sugar binding lectin protein and C-terminal domain of sialic acid synthase, respectively. Type IV is assumed to form a four-helix bundle which has sequence similarity with apolipoprotein. The results of our modeling suggest an ice-binding induced structural change of Type IV AFP. Insect AFP has ${\beta}$-helical structure with a regular array of Thr-X-Thr motif. Threonine residues of each Thr-X-Thr motif fit well into the ice crystal lattice and provide a good surface-surface complementarity. This review focuses on the structural characteristics and details of the ice-binding mechanism of antifreeze proteins.

High-Voltage GaN Schottky Barrier Diode on Si Substrate Using Thermal Oxidation (열 산화공정을 이용하여 제작된 고전압 GaN 쇼트키 장벽 다이오드)

  • Ha, Min-Woo;Roh, Cheong-Hyun;Choi, Hong-Goo;Song, Hong-Joo;Lee, Jun-Ho;Kim, Young-Shil;Han, Min-Koo;Hahn, Cheol-Koo
    • Proceedings of the KIEE Conference
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    • 2011.07a
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    • pp.1418-1419
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    • 2011
  • 차세대 전력 반도체인 고전압 GaN 쇼트키 장벽 다이오드의 역방향 특성을 개선하기 위해서 열 산화공정이 제안되었다. AlGaN/GaN 에피탁시 위에 쇼트키 장벽 다이오드 구조가 제작되었으며, 쇼트키 컨택은 증착 후 $450^{\circ}C$에서 산화되었다. 열 산화공정이 메사 측벽의 AlGaN 및 GaN 표면에 $AlO_x$$GaO_x$를 형성하여 표면으로 흐르는 누설전류를 억제한다. 표면 및 GaN 버퍼를 통한 누설전류는 열 산화 공정 이후 100 ${\mu}m$-너비당 51.3 nA에서 24.9 pA로 1/2000 배 수준으로 감소하였다. 표면 산화물 형성으로 인하여 생성된 Ga-vacancy와 Al-vacancy는 acceptor로 동작하여 surface band bending을 증가시켜 쇼트키 장벽 높이를 증가시킨다. 애노드-캐소드 간격이 5 ${\mu}m$인 제작된 소자는 0.99 eV의 높은 쇼트키 장벽 높이를 획득하여, -100 V에서 0.002 A/$cm^2$의 낮은 누설전류를 확보하였다. 애노드-캐소드 간격이 5에서 10, 20, 50 ${\mu}m$로 증가되면 소자의 항복전압은 348 V에서 396, 606, 941 V로 증가되었다. 열 산화공정은 전력용 GaN 전자소자의 누설전류감소와 항복전압 증가를 위한 후처리 공정으로 적합하다.

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Adequate anesthetic induction dose in a morbidly obese patient based on bioelectrical impedance analysis. -Case report- (병적 비만 환자에서 생체 전기 임피던스 분석을 이용한 적절한 마취 유도 용량 -증례보고-)

  • Lee, Ki-Jae;Choi, Seungseo;Baek, Seon Ju;Kim, Dong-Chan;Lee, Jeongwoo;Lee, Jun Ho
    • Journal of the Korea Convergence Society
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    • v.11 no.10
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    • pp.349-353
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    • 2020
  • Background: The dosage of the anesthetic drugs is generally determined by the total body weight of the patients. However, the drugs can be overdosed when the patient is morbidly obese. We have determined anesthetic induction dose based on lean body mass estimated from bioelectrical impedance analysis (BIA). Case: We report a case of morbidly obese patient (161 cm, 138 kg and body mass index 53.1) who had an elective laparoscopic cholecystectomy. The dose of induction agent was determined by lean body mass estimated by BIA, and the sedation was assessed by the observer's assessment alertness/sedation scale. Conclusions: Dose determination through lean body mass measured by BIA is useful in highly obese patients.