• Title/Summary/Keyword: 이용가능 시간과 불가능시간

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Molecular Analysis of Pathogenic Molds Isolated from Clinical Specimen (임상검체에서 분리된 병원성 사상균의 분자생물학적 분석)

  • Lee, Jang Ho;Kwon, Kye Chul;Koo, Sun Hoe
    • Korean Journal of Clinical Laboratory Science
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    • v.52 no.3
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    • pp.229-236
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    • 2020
  • Sixty-five molds isolated from clinical specimens were included in this study. All the isolates were molds that could be identified morphologically, strains that are difficult to identify because of morphological similarities, and strains that require species-level identification. PCR and direct sequencing were performed to target the internal transcribed spacer (ITS) region, the D1/D2 region, and the β-tubulin gene. Comparative sequence analysis using the GenBank database was performed using the basic local alignment search tool (BLAST) algorithm. The fungi identified morphologically to the genus level were 67%. Sequencing analysis was performed on 62 genera and species level of the 65 strains. Discrepancies were 14 (21.5%) of the 65 strains between the results of phenotypic and molecular identification. B. dermatitidis, T. marneffei, and G. argillacea were identified for the first time in Korea using the DNA sequencing method. Morphological identification is a very useful method in terms of the reporting time and costs in cases of frequently isolated and rapid growth, such as Aspergillus. When molecular methods are employed, the cost and clinical significance should be considered. On the other hand, the molecular identification of molds can provide fast and accurate results.

Measurement of Joint-Orientation and Monitoring of Displacement in Tunnel using 3D Laser Scanning System (3차원 레이저 스캐닝 시스템을 이용한 불연속면의 방향성 측정과 터널 변위 모니터링)

  • Shon, Ho-Woong;Oh, Seok-Hoon;Kim, Young-Kyung
    • Journal of the Korean Geophysical Society
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    • v.9 no.1
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    • pp.47-62
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    • 2006
  • More than 70% of Korean Peninsula is consisted of mountains, so that lots of roads, rail-roads and tunnel,which play a pivotal role in the industry activity, are existed along the rock-slope and in the rock-mass. Thus,it is urgent that tegration of management system through the optimum survey and design of rock-slope excavation, proper stabilization method and database of rock-slope. However, conventional methods have shortcoming with the economy of survey time and human resources, and the overcome of difficulties of approach to the in-situ rock-slope. To overcome the limitation of conventional method, this paper proposed the development of remote measurement system using Terrestrial Laser Scanning System. The method using Terrestrial 3D Laser Scanning System, which can get 3D spatial information on the rock-slope and2)Dept. Geosystem Engineering, Kangwon National University, Korea tunnel, has an advantage of reduction of measurement time and the overcome of difficulties of approach to the in-situ rock-slope/dam/tunnel. In the case of rock-slope, through the analysis of 3D modeling of point-cloud by Terrestrial Laser Scanning System, orientation of discontinuity, roughness of joint surface, failure shape and volume were successively achieved. in the case of tunnel face, through reverse-engineering, monitoring of displacement was possible.

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Removal of Nitrogen and Phosphorus Using Struvite Crystallization (Struvite 결정화에 의한 질소 및 인의 제거)

  • Weon, Seung-Yeon;Park, Seung-Kook;Lee, Sang-Ill
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.4
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    • pp.599-607
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    • 2000
  • In this research, ${NH_4}^+-N$ and ${PO_4}^{3-}-P$ in wastewater were removed by crystallization. Nitrogen and phosphate have been regarded as key nutrients in the eutrophication of rivers and lakes. Struvite, $MgNH_4PO_4{\cdot}6H_2O$, is insoluble in alkaline solutions. Fertilizer industry wastewater contains organic and nitrogen concentration of 330 mg/L and 550 mg/L, respectively. Nitrogen in this wastewater cannot be treated by conventional biological treatment without physicochemical pretreatment, because nitrogen concentration is relatively high compared to organic concentration. Magnesium ions used in this study were from bittern and commercial magnesium salts of $MgCl_2$ and $Mg(OH)_2$. Bittern obtained as a by-product of seasalt manufacture contains $8,000mg\;Ca^{2+}/L$ and $32,000mg\;Mg^{2+}/L$. Optimum initial pH was 10.5~11.0 and the reaction was complete or done in 2 min. Nitrogen removal efficiency using bittern, $MgCl_2 $ and $Mg(OH)_2$ (as source of $Mg^{2+}$) was 71 %, 81% and 83%. respectively. Phosphate removal efficiency was 99%, 98% and 93%, respectively. Therefore, bittern, $MgCl_2$ and $Mg(OH)_2$ can be efficiently used as $Mg^{2+}$ source for crystallization of nitrogen and phosphate. However, bittern is economically favorable $Mg^{2+}$ source for removing nitrogen and phosphate in wastewater.

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A Study on the One-Way Distance in the Longitudinal Section Using Probabilistic Theory (확률론적 이론을 이용한 종단면에서의 단방향 이동거리에 관한 연구)

  • Kim, Seong-Ryul;Moon, Ji-Hyun;Jeon, Hae-Sung;Sue, Jong-Chal;Choo, Yeon-Moon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.12
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    • pp.87-96
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    • 2020
  • To use a hydraulic structure effectively, the velocity of a river should be known in detail. In reality, velocity measurements are not conducted sufficiently because of their high cost. The formulae to yield the flux and velocity of the river are commonly called the Manning and Chezy formulae, which are empirical equations applied to uniform flow. This study is based on Chiu (1987)'s paper using entropy theory to solve the limits of the existing velocity formula and distribution and suggests the velocity and distance formula derived from information entropy. The data of a channel having records of a spot's velocity was used to verify the derived formula's utility and showed R2 values of distance and velocity of 0.9993 and 0.8051~0.9483, respectively. The travel distance and velocity of a moving spot following the streamflow were calculated using some flow information, which solves the difficulty in frequent flood measurements when it is needed. This can be used to make a longitudinal section of a river composed of a horizontal distance and elevation. Moreover, GIS makes it possible to obtain accurate information, such as the characteristics of a river. The connection with flow information and GIS model can be used as alarming and expecting flood systems.

Technology Trends and Future Prospects of Satellite-Based Photovoltaic Electricity Potential (위성기반 태양광 발전가능량 산출기술 개발 동향 및 향후 전망)

  • Han, Kyung-Soo;Kim, Jinsoo
    • Korean Journal of Remote Sensing
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    • v.32 no.6
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    • pp.579-587
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    • 2016
  • To obtain a stable energy supply and manage PhotoVoltaic (PV) systems efficiently, satellite imagery methods are being developed to estimate the solar PV potential. This study analyzed trends in the use of satellite imagery in solar PV and solar irradiation estimation technology. The imaging technology is used to produce solar energy resource maps. The trend analysis showed that the level of solar PV technology in Korea is 30% below that of advanced countries. It is impossible to raise such low-level technologies to the levels of advanced countries quickly. Intensive research and development is the only way to achieve the 80% technology level of advanced countries. The information produced in this process can contribute to the management of solar power plants. A valid technology development strategy would be to obtain effective data that can be used for fieldwork. Such data can be produced by estimating solar irradiation very accurately with several-hundred-meter resolution using Communication, Ocean, and Meteorological Satellites (COMS) and next-generation GEO-KOMPSAT 2A, developing core technologies for short- and medium-term irradiation prediction, and developing technologies for estimating the solar PV potential.

Deep-sea floor exploration in the East Sea using ROV HEMIRE (무인잠수정 해미래 활용 동해 저서환경 심해탐사)

  • Min, Won-Gi;Kim, Jonguk;Kim, Woong-Seo;Kim, Dong-Sung;Lee, Pan-Mook;Kang, Jung-Hoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.4
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    • pp.222-230
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    • 2016
  • HEMIRE is a 6,000-meter-class remotely operated vehicle (ROV) that has been developed for observation and sampling of objects of interest on the deep seabed. We first carried out deep-seabed exploration around the slopes of the Hupo Bank and the Ulleung Basin in the East Sea in June 2015. Over two weeks, a total of 10 dives were made from a support ship, the R/V Onnuri, at eight stations with water depth ranging between 194 and 2,080 m. The dive times ranged from 1 to 6 hours, depending on the operating conditions. We obtained the following results: 1) video images of the deep seafloor; 2) red snow crab density data (a major fishery resource) and inventories of deep-sea fauna, including an unrecorded organism; 3) specific topographies such as canyons slopes; 4) an undisturbed sediment core obtained using a push corer; and 5) observations of the seabed surface covered with discarded anthropogenic waste material.

Capillary Electrophoretic Method for the Determination of (+)-Catechin, (-)-Epicatechin in Grape Seed Ethanol Extract (포도종실 에탄올 추출물에 함유된 (+)-카테킨, (-)-에피카테킨의 모세관 전기영동법에 의한 분석)

  • Choi, One-Kyun;Chung, Yang-Seop;Chung, Ha-Yull
    • Korean Journal of Food Science and Technology
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    • v.37 no.4
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    • pp.513-518
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    • 2005
  • Capillary electrophoresis (CE) method was developed to determine (+)-catechin and (-)-epicatechin contents in grape seed ethanol extract. CE separation was achieved using 100 mM phosphate and borate buffer at pH 6.0 as background electrolyte and fused silica capillary with 50 microns x 375 microns O.D. (effective length 20.0cm) maintained at $25^{\circ}C$. The applied voltage was 10kV, and detection was performed by DAD at 210 nm, Two catechins were well separated within 6 min with repeatability of <0.8% RSD for migration time and <2.0% RSD for peak area, and correlation coefficients higher than 0.994 were obtained from 58.0 to 174.0 mg/L with detection limit of 0.035 mg/L. Separated compounds were successfully determined. CE method was easy to handle and showed good reproducibility. CE method was compared with conventional coloring and HPLC methods, and main advantages of CE method were low amount of sample required, simple pre-sample treatment, good recovery rate, and short analysis time.

Integrated Security Manager with AgEnt-based vulnerability scanner automatically generating vulnerability analysis code(ISMAEL) (취약성 점검 코드를 자동으로 생성하는 에이전트를 통한 통합 취약성 분석 시스템)

  • 김수용;서정석;조상현;김한성;차성덕
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.12 no.2
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    • pp.111-122
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    • 2002
  • Malicious attackers generally attempt to intrude the target systems by taking advantage of existing system vulnerabilities and executing readily available code designed to exploit blown vulnerabilities. To the network security administrators, the rat and minimal step in providing adequate network security is to identify existing system vulnerabilities and patch them as soon as possible. Network-based vulnerability analysis scanners (NVAS), although widely used by network security engineers, have shortcomings in that they depend on limited information that is available and generally do not have access to hast-specific information. Host-based vulnerability analysis scanner (HVAS) can serve as an effective complement to NVAS. However, implementations of HVAS differ from one platform to another and from one version to another. Therefore, to security engineers who often have to maintain a large number of heterogeneous network of hosts, it is impractical to develop and manage a large number of HVAS. In this paper, we propose an agent-based architecture named ISMAEL and describe its prototype implementation. Manager process provides various agent processes with descriptiom on vulnerabilities to check, and an agent process automatically generates, compiles, and executes an Java code to determine if the target system is vulnerable or not. The result is sent back to the manager process, and data exchange occurs in % format. Such architecture provides maximal portability when managing a group of heterogeneous hosts and vulnerability database needs to be kept current because the manager process need not be modified, and much of agent process remains unchanged. We have applied the prototype implementation of ISMAEL and found it to be effective.

Application of Equivalent Beam Element for Practical Vibration Analysis of Stadium Structure (스타디움 구조물의 실용적인 진동해석을 위한 등가보요소의 적용)

  • Kim, Gee-Cheol
    • Journal of the Earthquake Engineering Society of Korea
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    • v.8 no.5 s.39
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    • pp.91-99
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    • 2004
  • In general, stadium structure with long span has low inherent natural frequency. In the stadium structure, structural behavior similar to resonance can be occurred easily by spectator rhythmical movements of which exciting period is small comparatively. It is required to investigate the safety and the serviceability of stadium structure. Therefore, there exists a necessity for accurate vibration analysis. Accurate analysis of stadium structure subjected to dynamic load is required for economical construction and safe design of stadium structure. Stadium structure should be modeled by refined mesh for accurate vibration analysis. As the mesh of stadium structure is refined, the number of divided elements increases in numerical analysis. The number of node is increased and numerous computer memories or computational time are required. So it is very difficult to analyze refine model of stadium structures by using the commercial programs. It is possible to efficient vibration analysis of stadium structure by finite element modeling method using equivalent beam element proposed in this paper, because the number of nodes is decreased remarkably.

A Design and Implementation of the Realtime Video Lecture System for Multimedia Education (멀티미디어 교육을 위한 실시간 영상강의 시스템의 설계와 구현)

  • 방혜자
    • Journal of Korea Multimedia Society
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    • v.5 no.6
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    • pp.625-637
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    • 2002
  • The Internet had been taken properties which were both a slow speed and low-bandwidth of the first time. It was impossible to transmit large multimedia data because of a Internet properties. After tens of Year Passed, many people began to transmit multimedia data in the Internet which had fast-speed and high-bandwidth. Some people want to team classes to be taught in school through the Internet. And, educators wish to use multimedia data in the educational field. Due to previous reasons, many parts in the educational field had tried to join real-time objects to multimedia objects. But, real-time data didn’t appear easily due to many faults like network errors and system bugs if we want to control data in order to teach and learn classes. Then, this paper separates real-time and unreal-time data and controls these data that aye made a time scheduler to have time information in the extended SMIL-file for deceasing these faults.

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