Vitrification of oocytes has been applied recently fur pigs, but remains elusive. The purpose of this study is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine oocytes. When immature follicular oocytes frozen-thawed were cultured for in vitro maturation, maturation rates to metaphase-II stage were higher in oocytes with (25%) than without (15%) cumulus cells. After In vitro fertilization of oocytes frozen-thawed, the maturation rates were also significantly (P<0.05) higher in oocytes with (41%) that than without (17%) cumulus cells. However, the penetration rates were higher in oocytes without (19%) that than with (9%) cumulus. In another experiment, porcine oocytes matured in vitro were frozen and thawed for in vitro fertilization. The penetration rates were higher than in oocytes without (35%) that than with (26%) cumulus cells. However, the proportions of oocytes dead after in vitro fertilization were significantly (P<0.05) higher in oocytes with that than without cumulus cells. On the other hand, the rates of penetration and dead oocytes at 6 h after in vitro fertilization were not significant differences between oocytes with and without cumulus cells. However, the proportions of dead oocytes with (18%) and without (16%) cumulus cells were higher than in oocytes of control group (0%). These finding indicated the possible broader application for OPS, as they demonstrated that the maturation and fertilization in vitro by frozen-thawed oocytes may be accompained by cumulus cells and culture periods according to the requirements of the survival ability after freezing of mature and immature oocytes in pigs.
We investigated the reproductive cycle of Corbicula japonica with its gonadal development by histological observations, and the seasonal changes in biochemical mass and foot muscle of the adductor muscle, visceral mass and foot muscle of the clam by biochemical analysis, from January to December, 2003. The reproductive cycle of this species can be classified into five successive stages: early active stage (February to April), late active stage (April to July), ripe stage (June to August), partially spawned stage (July to September) and spent/inactive stage (September to March). According to ANOVA test, there were significant differences (p < 0.05) in total protein, total lipid and glycogen contents among months for all of the visceral mass, adductor muscle and foot muscle. Total protein content was highest in adductor muscle, while lowest in visceral mass. There was no correlation in total protein content between visceral mass and adductor muscle (p = 0.208). However, strong positive correlation was found between adductor muscle and foot muscle (r = 0.769, p < 0.001). In visceral mass, total lipid content was the highest; it was 2 or 3-fold higher than in adductor muscle or foot muscle. The monthly change was also most dynamic in visceral mass. It decreased from January to March (early active stage), and reached maximum in April (late active stage). From May to August (ripe and partially spawned stage), it dradually decreased and then increased again until October (spent/inactive stage). Multiple comparisons showed that total lipid content in visceral mass between all of the adjacent two months was significaltly different (p < 0.05). There were strong negative correlations in total lipid content between visceral mass and adductor muscle (r = 0.687, p < 0.001), and between visceral mass and foot muscle (r = 0.473, p = 0.008). Changes of glycogen content were more or less similar to the changes of lipid contents in visceral mass, adductor muscle and foot muscle, except for April. In April, glycogen content in visceral mass was over four times higher than that in adductor muscle or foot muscle. There was a positive correlation in glycogen content between adductor muscle and foot muscle (r = 0.686, p < 0.001). Especially, total lipid content showed a negative correlation between the adductor muscle and visceral mass. Therefore, these results indicate that the nutrient content of the adductor muscle, visceral muscle and foot muscle changed in response to gonadal energy needs.
Park, Ji-Youn;Choi, Mi-Youn;Lee, Seon-Heui;Choi, Yoon-Ho;Park, Yoo-Kyoung
Journal of Nutrition and Health
/
v.44
no.1
/
pp.29-40
/
2011
The purpose of this study was to examine the association among bone mineral density (BMD), biochemical bone markers, nutrients, and salt intake in premenopausal and postmenopausal women. We evaluated 431 subjects who visited a health promotion center of a university hospital between January 2008 and July 2009. We excluded those who were taking medications or who had an endocrine disorder affecting osteoporosis. The subjects were divided into premenopausal (n = 283) and postmenopausal (n = 143) women. We evaluated the correlation among BMD of the lumbar spine, femoral neck, and total femoral, as well as biochemical bone markers, hormone, serum profiles, general characteristics, nutrient intakes, and food intake frequencies. From a stepwise multiple regression analysis, lumbar spine BMD was positively correlated with weight (p < 0.001) and negatively correlated with osteocalcin (OC)(p < 0.001), Femoral neck BMD was positively correlated with weight (p < 0.001) and negatively correlated with C-telopeptide (CTx) and alkaline phosphatase (ALP)(p < 0.001, p < 0.05). In premenopausal women, femoral total BMD was positively correlated with BMI (p < 0.001) and negatively correlated with CTx (p < 0.001). In postmenopausal women, lumbar spine BMD was positively correlated with calcium intake (p < 0.01) and negatively correlated with sodium intake (p < 0.01). Femoral neck and femoral total BMD were both positively correlated with weight (p < 0.001), and femoral neck BMD was negatively correlated with age and ALP (p < 0.001, p < 0.05). Femoral total BMD was negatively correlated with age and OC (p < 0.001, p < 0.01). These results suggest that reducing sodium intake may play an important role delaying bone resorption and preventing a decrease in BMD.
This study is a descriptive research study to grasp the critical thinking tendency, clinical practice stress, core basic nursing confidence, and degree of adaptation to clinical practice in nursing students. From June to July 2019, 207 students in the fourth year of nursing college were surveyed through their own questionnaire. Collected data were analyzed by t-test, ANOVA and multiple regression analysis using SPSS/WIN v24.0. The negative correlation between clinical practice stress and critical thinking tendency(r=-.18, p=.010). The core self-confidence practice self-confidence showed a positive correlation with critical thinking tendency(r=.25, p<.001), and a negative correlation with clinical practice stress(r=-.17, p=.017). Adaptation to clinical practice showed a positive correlation with clinical practice stress(r=.44, p<.001), and the factor influencing clinical practice adaptation was stress(β=.40) (F=18.34, p<.001), and the explanatory power was about 23.1%. As mentioned above, stress brings a positive change to clinical practice, so it is necessary to identify stress factors received from clinical practice of nursing college students and reflect them in curriculum development.
This study analyzed the effects of wage workers' job satisfaction, life satisfaction on turnover intention, and moderating effect of employment type after the global financial crisis using Korean Labor and Income Panel Study data. As a result, we found that the higher the job satisfaction is, the lower the turnover intention. However, the higher the job satisfaction and life satisfaction are, the higher the turnover intention. This is because the more satisfied with the job environment, the higher the tendency to seek better job opportunities, or the change in occupational value that favored free work. The higher the age, the higher the educational level, and the regular jobs are, the turnover intention decreased. In addition, the interaction terms of job satisfaction, job environmental satisfaction, life satisfaction, and regular employment all showed a moderating effect of employment type. However, since the results of the relationship between job satisfaction, job environmental satisfaction, life satisfaction and turnover intention, and the moderating effect may be due to limitations of measurement variables, further study is needed.
Journal of the Korean Society of Food Science and Nutrition
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v.25
no.6
/
pp.1055-1061
/
1996
This study was conducted to assess meal management and nutrient intake of 357 elderlies in Ulsan. 114 male and 243 female elderlies over 65 year old were recruited from July 5 to August 13, 1995. Questionnaires for general characteristics of the subjects(such as family size, living cost, pocket money and health condition) and for meal management were used. The levels of depression and anxiety were measured by CED-S and Spielbuger's STAI-S, respectively In addition, nutrient intakes were conducted by convenience methods. Nutrient intake of male and female showed the average daily intake of energy, Protein, vitamin A, vitamin $B_2, $ and vitamin C were lower than Korean recommended dietary allowances(RDA). But other nutrient intake were higher than RDA except that calcium intake of female was lower. Most nutrient intakes were positively correlated with family size, living cost, pocket money and health condition. Depression and anxiety scores were also negatively correlated with most of nutrient intakes. The pattern of meal management could predict nutrient intakes as expected : regularity and pleansantness of eating were positively correlated with nutrient intakes, whereas unbalanced meal and eating lonely were negatively correlated with them.
Improved procedures were implemented in the production of the lithospheric magnetic anomaly map from Magsat satellite magnetometer data of East Asia between $90^{\circ}E-150^{\circ}E$ and $10^{\circ}S-50^{\circ}N$. Procedures included more effective selection of the do·it and dawn tracks, ring current correction, and separation of core field and external field effects. External field reductions included an ionospheric correction and pass-by-pass correlation analysis. Track-line noise effects were reduced by spectral reconstruction of the dusk and dawn data sets. The total field magnetic anomalies were differentially-reduced-to-the-pole to minimize distortion s between satellite magnetic anomalies and their geological sources caused by corefield variations over the study area. Aeromagnetic anomalies were correlated with Magsat magnetic anomalies at the satellite altitude to test the lithospheric veracity of anomalies in these two data sets. The aeromagnetic anomalies were low-pass filtered to eliminate high frequency components that may not be shown at the satellite altitude. Although the two maps have a low CC of 0.243, there are many features that are directly correlated (peak-to-peak and trough-to-trough). The low CC between the two maps was generated by the combination of directly- and inversely-correlative anomaly features between them. It is very difficult to discriminate directly, inversely, and nully correlative features in these two anomaly maps because features are complicatedly correlated due to the depth and superposition of the anomaly sources. In general, the lithospheric magnetic components were recovered successfully from satellite magnetometer observations and correlated well with aeromagnetic anomalies in the study area.
The purpose of this study was to identify the factors affecting job satisfaction in dental hygienists with and without schoolwork, who form professional manpower in dental clinics, and provide basic data that could help develop detailed plans for reducing the turnover intention and improving job satisfaction. For data collection, convenience sampling was performed among clinical dental hygienists at dental clinics in Daejeon and Chungcheong and Gyeongsang Provinces from July 20 to September 20, 2019, and a self-administered questionnaire was used and the data were analyzed by T-test, One-way Anova, Pearson correlation, Stepwise multiple regression analysis, etc. using IBM SPSS/WIN 20.0. For the dental hygienists with schoolwork, job stress was negatively correlated with professional self-concept and job satisfaction, and professional self-concept was positively correlated with job satisfaction; for the dental hygienists without schoolwork, job stress was negatively correlated with professional self-concept and job satisfaction, and professional self-concept was positively correlated with job satisfaction. The factors affecting job satisfaction were professional self-concept, job stress, and age of ≥30 for the dental hygienists with schoolwork and professional self-concept, job stress, and the position as a middle manager for those without schoolwork. To put these results together, professional self-concept and job stress were factors affecting job satisfaction for dental hygienists with and without schoolwork. It is necessary to focus on the formation of good dental organizational culture through positive institutional support as well as through systematic establishment of professional self-concept.
A field survey and Landsat ETM+ image acquisition carried out simultaneously. Using these data, we attempted to establish relationships between tidal flat environmental factors and reflectance observed by ETM+, and to set up a new critical grain size useful for optical remote sensing. Although the grain size of 4 $\Phi$ has been conventionally used as a critical size by sedimentologists, the correlation with optical reflectance was very low. Instead, the grain size of 2 $\Phi$ showed a relatively high correlation coefficient, 0.699, with ETM+ band 4, except near tidal channels in upper tidal flat. We concluded that the grain size of 2 $\Phi$ would be better to use for a critical grain size in Gomso Bay. The grain size also correlated well with moisture content having a correlation coefficient of -0.811 when the 2 $\Phi$ criterion was used. The results of factor analysis showed moisture content was more important parameter than topographic relief, and they were different from German tidal flats in which topographic relief was the prior factor This can be explained by finer grain composition of the Gomso bay tidal flat. In short, moisture content and topography as well as grain size should be considered in tidal flat remote sensing.
In this study, analyzed the effect of the introduction and operation of internal control system of a company on accounting information usefulness in a rapidly changing business environment. In order to conduct this study, we studied a comprehensive analysis on the usefulness of internal control and accounting information, and applied the research model to the purpose of this study. In this study, we analyzed the degree of the relationship between variables based on the discretionary accruals of the modified Jones model using the internal control evaluation index based on the components of the internal control system for China. In the empirical analysis, analyzed that the operation of internal control has a negative influence on discretionary accruals, which is a substitute for usability of accounting information. In addition, the risk management factors of the internal control system have a negative correlation with the usefulness of accounting information. The results of this study suggest that it is possible to present the positive function of internal control system to many firms where the introduction and operation of internal control is important, and to provide useful guidance in the study of the relationship between the operation of internal control and discretionary accruals for foreign company.
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