• Current Research on Agriculture and Life Sciences
• /
• v.6
• /
• pp.13-18
• /
• 1988

This experiment was conducted to identify the enzyme treatment time, calcium ion effect, enzyme concentration and leaf position for protoplast isolation. It was also performed to determine the adequate molarity on protoplasts, and to investigate the incubation time, pH, PEG concentration and DMSO effect for protoplast fusion. The results obtained were summarized as follows ; The optimal time of incubation in enzyme solution was 4 hours. And the protoplast releasing time was delayed by $CaCl_2{\cdot}2H_2O$ addition to the enzyme solution compared with no added one. The viability had kept up to above 95% until the 4 hours after digestion. The high viability of the protoplast was preserved more than 16 hours by adding $CaCl_2{\cdot}2H_2O$ to digestion solution. The enzyme concentration had no effect on protoplast yield in range from 1% to 5% and the first or second leaf from the top of the plant produced the highest protoplast yield among the leaf position tested. The purity of healthy protoplast was better in 0.4M and 0.5M sucrose than in others, and the percentage of protoplast aggregation was more 20% to 50% in PEG 6,000 compared with 4,000 and PEG 1,500. Even though the percentage of protoplast aggregation was less increased by 3% to 7% than without DMSO, its treatment was effective to induce binucleated protoplasts.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.21 no.1
• /
• pp.121-129
• /
• 2021

In addition to yellow dust occurring in China, fine dust has become a hot topic in Korea through news and media. Although there is fine dust generated from the outside, the purchase rate of air purifier products is increasing as external fine dust flows into the inside. The air purifier uses a filter internally, and the sensor notifies the user through the LED alarm whether the filter is replaced. However, there is currently no product measuring how much the filter rate is reduced and determining the pressure of the blower to operate. Therefore, in this paper, data are generated directly using Arduino, fine dust sensor, and differential pressure sensor. In addition, a program was developed using Python programming to calculate how old the filter is and to analyze the wind power of the blower according to the filter rate by calculating the measured dust and pressure values.

• Journal of Embryo Transfer
• /
• v.20 no.3
• /
• pp.207-215
• /
• 2005

본 연구는 돼지 난포란의 동결보존 후 생존성과 난자의 활성화 처리에 따른 체외발생율과 이를 이용한 핵 이식배의 체외발생율을 조사하였다. 활성화 처리된 배는 $5\%$ FBS가 첨가된 NCSU 23 배양액으로 $38.5^{\circ}C$, $5\%\;CO_2$와 $95\%$ air의 조건으로 배양하였다. 1. 난포란을 EDS와 $5\%$ PVP로 동결 후 $10\%$ FBS가 첨가된 NCSU 23 배양액으로 $0{\~}10$시간 배양했을 때 체외발생율은 $36.0\%$로서 대조군인 비동결 난포란의 체외발생율 $46.0\%$에 비해 낮았다. 2. Ethanol과 cyclojexamide로 처리 후 42 및 46시간 배양한 배의 분할율은 각각 $33.3\%$, $36.0\%$ 및 $27.1\%$, $30.0\%$로서 대조군의 $8.8\%$, $11.4\%$에 비해 높게 나타났다. 3. 동결 및 비동결 난포란을 이용한 핵이식 배의 융합율과 발생율 간에는 유의한 차이가 없었다. 4. Ethanol과 cyclojexamide로 활성화 처리한 난자를 이용하여 재구축한 핵 이식배의 발생율은 $2.8\%$, $5.3\%$ 및 $1.5\%$, $2.9\%$로서 대조군의 $0.0\%$, $0.0\%$에 비해 높은 발생율을 나타냈다.

• Korean Journal of Animal Reproduction
• /
• v.27 no.1
• /
• pp.87-93
• /
• 2003

This study was conducted to investigate the effects of embryo development by fusion condition on the nuclear transfer with brindle coated cow's ear cells. Ear cells were transferred into an enucleated oocyte and fused with cytoplasm in the fusion condition with 1.9kv/cm, 2.0kv/cm, 2.1kv/cm each 10 and 20ug duration Nuclear transfer embryo were activeted with a combination of 5ug/ml and 1.9mM 6-DMAP (4min, 4h). Fusion rate was 51∼68% range among fusion condition (1.9, 2.0, 2.1kv/cm; 10, 20us). But, cytoplasm lysis rate was increased by higher electric condition (0∼51.8% range). Each parameter's cleavage and blastocyst formation rate were 1.9kv/cm for 10us (75.8 and 19.5%), 20us (69.8 and 48.6%), 2.0kv/cm for 10us (76.9 and 20.0%), 20 us (68.5 and 40.9%), 2.1kv/cm for 10us (70.5 and 44.2%), 20 us (68.5 and 27.0%). We compared the effectiveness of cloning for between brindle coated cow's ear cells and Hanwoo fetal fibroblast cells. There was no significant differences in the fusion rate and developmental rate to the blastocyst stage. After transfer of blastocysts derived from nuclear transfer embryos, pregnancy rates of the Hanwoo fetal fibroblast cells and brindle coated cow's ear cells were checked pregnant on day 60 as assessed by ultrasonography, 40% (2/5) and 15.8% (3/19), respectively. This studies conclude that brindle coated cow's ear cells have the developmental potentiality to term by nuclear transfer. These results demonstrate that the increased the field strength was to be profitable for development of blastocyst or reduce of cytoplasm's damage than increasing the pulse duration.

• Journal of the Korea Convergence Society
• /
• v.10 no.6
• /
• pp.347-353
• /
• 2019

This study was a descriptive research to investigate the difference of obesity, smoking, drinking affected allergy disease in Korean adolescents using the raw data of $6^{th}$ Korea National Health and Nutrition Survey(2015). Study data on 535 Korean adolescents classified 12 to 18 years and Rao-Scott Chi-square test and logistic regression were used for analysis. The results of the study showed that the prevalence of asthma, atopic dermatitis, allergic rhinitis were 5.4%, 15.5%, 22.8% and not shown differences according obesity, smoking, drinking. But, the risk factor of asthma was related obesity, atopic dermatitis was related smoking. Based of this study's findings, It is necessary to develop the Heath Promotion Intervention Program according of obesity, drinking, smoking for the quality of life in adolescents diagnosed Allergy disease.

• Microbiology and Biotechnology Letters
• /
• v.36 no.2
• /
• pp.158-164
• /
• 2008

The fusants which contain starch utilizing ability and alcohol fermenting ability were developed by protoplast fusion of Saccharomyces cerevisiae KOY-1 and Saccharomyces diastaticus KCTC 1804. Sacharomyces cerevisiae KH-12 was obtained by haploid induction from Saccharomyces cerevisiae KOY-1. The auxotropic mutants of yeast were obtained by using an ethylmethane sulfonate (EMS). The frequency of protoplast formation in Saccharomyces cerevisiae KOY-1 $(Met^-)$ and Saccharomyces diastaticus KCTC 1804 $(Trp^-)$ were 90.5% and 97.7%, respectively. The frequency of fusant formation was $1.79{\times}10^{-4 }$ for the regenerated protoplast and the 1,000 fusants were obtained. Fusant FA 776 was selected as a potential yeast which contain an alcohol fermenting ability in the starch medium. The genetic stability was 4.64% for 10 passages of generation. Fusant FA 776 produced 13mg/ml of alcohol in 24% starch medium and showed 1.86-fold higher alcohol fermenting ability than Saccharomyces diastaticus KCTC 1804.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.7 no.5
• /
• pp.847-851
• /
• 2006

This paper presents a technology of information fusion between radar and electronic intelligence system. Radar can get range and direction information of targets and electronic intelligence system can get direction and electromagnetic information of targets which can be fused and identified together. We designed an information fusion unit in which information data is able to be added and compared and designed a display unit in which a fused information is totally displayed.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2020.11a
• /
• pp.705-708
• /
• 2020

합성곱 신경망(Convolutional Neural Network) 기반 객체 탐지기의 발전으로 돈사에서 돼지 모니터링이 가능하지만, 실제 농가에서 적용하기 위해서는 탐지기의 정확도를 개선해야 하는 문제가 여전히 남아있다. 본 연구에서는 합성곱 신경망 기반 돼지 탐지기의 출력인 박스들의 신뢰도 값을 평가하고 잘못된 박스들의 신뢰도 값을 보정하는 박스 레벨 후처리 방법을 제안한다. 즉, 신뢰도 값이 가짜 돼지인지 진짜 돼지인지 애매한 경우, 박스내 전경 픽셀 정보와 인접 박스의 정보를 이용하여 신뢰도 값을 낮추거나 높이는 보정 작업을 수행한다. 그리고 실제 돈사에서 취득한 11,308장의 영상 데이터로 실험한 결과, 제안 방법은 합성곱 신경망 기반 돼지 탐지기의 에러율을 4.4%에서 1.2%로 개선하는 효과가 있음을 확인하였다.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2022.07a
• /
• pp.107-109
• /
• 2022

본 논문은 건설구조물 안전의 가장 중요한 요소 중 하나인 콘크리트 압축강도 안정성을 확보하기 위한 시스템으로, 콘크리트 구조물을 만들 재료인 레미콘의 수분 감소율에 따른 압축강도 감소 리스크를 관리할 수 있는 모델을 제시하였다. 동일한 물,시멘트비(W/C)로 생산된 레미콘은 현장타설시까지 교통환경으로 인한 도착시간 지연 및 강우, 강설 등 외부적인 요건으로 감수율이 발생하는 리스크가 발생한다. 이로 인해 콘크리트의 압축강도가 저감하는 중대한 문제가 발생한다. 본 연구에서 제시한 알고리즘을 이용하여 현장 타설전 콘크리트 시료의 함수율을 측정하여 감수율이 발생한 제품 발견시, 실시간으로 Operator로 GCM 기반의 Push Alarm을 전송하여 감수율이 반영된 제품을 제공함으로써 구조물의 안전성을 확보할 수 있는 시스템을 모델링하였다. 본 연구는 기존시스템의 문제점을 실시간으로 개선할 수 있는 것으로 건설현장의 구조물 안정성 확보에 효과가 클 것으로 기대된다.

• Journal of the Korea Convergence Society
• /
• v.12 no.7
• /
• pp.125-131
• /
• 2021

Blue light with strong energy is required for light-curing resin treatment, which is being used more frequently in dentistry. To reduce the risk of exposure to scattered light, we tried to use colored lenses. The tips for light curing machine and a commercially available yellow-type blue-light blocking lens and a yellow lens colored with yellow dye, which are expected to be effective in blocking blue light, were placed in a UV-Vis spectrometer device, and transmission and blocking of blue light were tested respectively. As a result, the average blue light blocking rate of the light curing machine tips was 99.49%, and the C lens with the highest color density among commercially available lenses showed a high blue light blocking rate of 99.54%. In the case of lenses tinted with yellow, the yellow tinted C lens with the highest tint concentration showed 87.57% of blue light blocking rate. It is judged that the side effects related to the eyes caused by blue light can be reduced if a yellow-type commercially available or colored lens is worn along with a light curing machine tip during resin treatment.