• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.116-116
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• 2003

도축 소의 난소를 이용하여 체외에서 수정란 생산과 이식이 산업화에 접어들고 있지만, 그 기원이 되는 난소의 자질은 검토되어 있지 않고, 생산된 송아지의 자질 또한 의문시 되고 있는 실정이다. 본 실험에서는 도축 한우의 육질과 육량에 따른 배발달율을 조사하여 고품질 체외수정란의 생산에 기초를 확립 하고자 실시하였다. 한우 난소는 도축 직후에 개체별로 paper에 싸서, 0.9%생리식염수 (25-$28}{\circ}C$)가 들어있는 보온병에 담아 실험실로 운반하였다. 운반된 난소의 2~8mm의 가시난포로부터 난포란을 회수하였다. 회수된 난포란은 10% FBS, 1$\mu g/ml$ FSH, 10$\mu g/ml$LH 그리고 1$\mu g/ml$ Estradiol-$17 \beta$가 첨가된 TCM199 용액에서 24시간 체외성숙을 실시하였다. 체외수정은 fer-TALP 용액을, 체외배양은 CR1aa 용액에서 배양 3일째까지는 0.3% BSA, 그 이후에는 10% FBS와 난관 상피세포를 첨가하여 사용하였다. 통계분석은 $X^2-test를 이용하였다. 도축 한우의 육질등급에 따른 수정율은 1, 2, 3 및 등외등급에서 각각 63.7, 82.7, 73.2 및 84.0%로서 등외등급에서 가장 높은 수정율을 나타냈다. 배반포까지 발달율도 각각 17.1, 32.2, 26.8 및 40.0%로서 등외등급에서 가장 높았으며 특히 등외등급의 배발달율이 1등급에 비하여 유의적(P<0.05)으로 높았다. 육량등급에 따른 수정율은 A, B, C 및 등외등급에서 각각 90.0, 62 0, 69.2 및 85.0%로서 A등급이 가장 높았고 배반포까지 발달율은 각각 21.2, 18.7, 22.5 및 20.2%로서 C등급이 가장 높았으나 유의적인 차이는 없었다. 이상의 결과에서 한우 난포란의 배발달에는 육량등급보다는 육질등급에 많은 영향을 받는 것으로 판단된다. 한편 육질 1등급에서 배발달율이 낮은 이유는 육질 향상을 목적으로 암소를 비육 하는 경우 발생하는 번식장애와 밀접한 관계가 있는 것으로 사료된다.

• Journal of the Korean Institute of Intelligent Systems
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• v.26 no.6
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• pp.433-438
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• 2016

This paper proposes a grade prediction method to measure meat quality in Hanwoo (Korean Native Cattle) using classification and feature extraction algorithms. The applied classification algorithm is an AdaBoost and the texture features of the given ultrasound images are extracted using SFTA. In this paper, as an initial phase, we selected ultrasound images of Hanwoo for verifying experimental results; however, we ultimately aimed to develop a diagnostic decision support system for human body scan using ultrasound images. The advantages of using ultrasound images of Hanwoo are: accurate grade prediction without butchery, optimizing shipping and feeding schedule and economic benefits. Researches on grade prediction using biometric data such as ultrasound images have been studied in countries like USA, Japan, and Korea. Studies have been based on accurate prediction method of different images obtained from different machines. However, the prediction accuracy is low. Therefore, we proposed a prediction method of meat quality. From the experimental results compared with that of the real grades, the experimental results demonstrated that the proposed method is superior to the other methods.

• KAPE Magazine
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• no.71
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• pp.8-10
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• 2002

• Journal of Animal Science and Technology
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• v.54 no.1
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• pp.29-33
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• 2012

This study was carried out to investigate carcass grade of pigs according to gender, feed intake, backfat thickness, carcass weight, etc. Data from a total of 26,983 heads in 5 farms were collected. The results obtained were as follows; The difference was significantly found in carcass quality grade between barrows and gilts (p<0.001). The meat grade $1^+$ was high in gilts, but meat grade 1 was high in barrows, respectively. The carcass weight of 85.8 kg was lightly shown significantly (p<0.05) in group I taken little quantity of feed consumption. However, that of 89.5 kg was heavily shown in group IV taken plenty of feed consumption. Meat grade 2 was significantly (p<0.001) high ratio in group of backfat thickness with less than 15 mm (67.5%). However, the ratio for the grade 1 was high in group of 15~19 mm. The ratio for the meat grade 2 was highly shown in group of carcass weight with more than 85 kg (54.3%). On the other hand, that of grade 1 was highly shown in group of 78~84 kg. In conclusion, pigs for marketing to enhance high quality have been appropriately raised with the optimum system in consideration of feed intake, carcass weight, backfat thickness, etc.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.10a
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• pp.123-126
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• 2005

본 연구는 고등동물의 불포화지방산 생합성의 핵심 효소로 알려져 있는 Stearoyl-CoA desaturase(SCD) 유전자의 특정한 단일염기다형(single nucleotide polymorphism; SNP)이 한우의 도체 및 육질형질에 미치는 영향을 분석하기 위해 SCD 유전자의 Intron 7번 영역의 특정부위를 포함하는 primer를 제작하고, 염기서열 분석을 통하여 유전자 구조를 해석한 결과 총 211bp 크기를 갖는 염기서열의 122번째에서 아데닌(A)${\leftrightarrow}$구아닌(G) 염기치환으로 발생한 단일염기다형(SNP) 부위를 발견하였다. 이들 단일염기다형 염기서열 부위를 PCR-SSCP(single-strand conformation polymorphism) 기법을 이용하여 분석한 결과 3종류의 SNP 유전자형(A/A, A/G 및 G/G)을 검출하였다. 이 가운데 A/G 유전자형이 한우의 근내지방도와 등지방두께와 고도의 유의적 연관성이 있다는 새로운 사실을 발견하였다. 따라서, 본 연구를 통해 개발된 한우 SCD 유전자의 특정한 단일 염기다형 표지인자는 한우의 연령 및 성별에 관계없이 육질이 우수한 고급육을 생산하는 우량 한우의 조기식별에 매우 유용한 DNA 표지인자로 활용할 수 있을 것으로 기대된다.

• Korean Journal of Food Science and Technology
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• v.28 no.4
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• pp.786-789
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• 1996

An image processing system was built to evaluate the color properties of apple and meat. The system consisted of video camera, video card, 32 bit microcomputer and an optical illuminator. The operating software was developed to carry out capturing, analyzing, displaying and storing of the 8 bit digitized images of food. The images of apples at various maturing stages were investigated to obtain the color histogram of R, G, B and Hunter value. RGB histogram showed a major difference in G value, 35.01, the minor change in R value, 6.16, and the negligible difference in B value. The image of beef cut was separated into two parts, fat and lean tissue, by applying threshold value method based on the digital value of color. The threshold value for fat was over 240 and for lean under 230 in R value, respectively. The resulting non fat image showed 2% decreased color difference value, ${\Delta}E$, than whole meat cut.

• Journal of Animal Science and Technology
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• v.55 no.3
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• pp.179-184
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• 2013

Willingness-to-pay (WTP) for pork produced by quality test was determined using a contingent valuation method. Three model studies including a dichotomous-choice and two double dichotomous-choice types were conducted. The respondents in this study appropriately understood the contingent valuation and the suggested price was significant as a characteristic variable. The results imply that there is lower chance to select pork produced by the quality test, as the price difference is greater between conventional and quality-tested pork. WTPs in double and single contingent valuation models were 735 and 547 won/100 g, respectively. WTP was increased with increasing the educational level of respondents. The average WTPs analyzed by convariate were 1,015 won/100 g for double contingent valuation and 580 won/100 g for single contingent valuation. Considering the minimum price of WTP of pork produced by quality test (547.4 won/100 g), the total economic value was estimated to be 5,173,600 million won and per capita customer value was 106,000 won. Therefore, providing an institutional strategy for pork quality test will be beneficial for the consumers.

• Microbiology and Biotechnology Letters
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• v.43 no.4
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• pp.322-329
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• 2015

The effects of water extracts of Gallus gallus var. domesticus (Yeonsan ogolgye, GD) on the activities of osteoblast differentiation and the restraint of osteoclast formation were investigated. The water extract of GD in the human osteoblast "MG-63" cell, was examined in relation to alkaline phosphatase (ALP) activity and alizarin red stains. In order to observe the effects of osteoclasts formation, we analyzed RAW 264.7 cell tartrate-resistant acid phosphatase (TRAP) activity and TRAP stains. The ALP activity of the water extract of hen and cock flesh (3 years) were 133.8% and 129.6%, respectively. The ALP activity of flesh extracts was also higher than that of the skin extracts. Concerning the effects of age, the 3 years old flesh extracts had a higher activity than that of the one year old extracts. However the activity of the 3 years old skin extracts was lower than that of the one year old extracts. For gender conditions, the ALP activity of the hen extract was higher than that of the cock. The degree bone mineralization in the three years old hen flesh exhibited the highest rate, at 124.3%, amongst all the groups. The TRAP activity of the flesh extracts of the three years old cock revealed the lowest rate, at 31.8%, compared to the control. Our results demonstrate that the water extract of GD increases bone mineralization and osteoblast differentiation activity in MG-63 cells and enhances the inhibitory activity of bone-resorption in RAW 264.7 cells. In conclusion, the water extracts of GD seem to be effective in the prevention and treatment of bone related disorders.

• Journal of Animal Science and Technology
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• v.53 no.2
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• pp.89-97
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• 2011

In this study, the efficiency of microsatellite (MS) markers for pork quality was examined and further, their suitability to domestic pork industry also was verified, by measuring meat quality parameters of Berkshire breeds. A total of 323 pigs of Berkshire breeds were slaughtered and subjected to meat quality evaluation. In addition, the genomic DNAs from blood samples of slaughtered pigs were used for genotyping analysis of 50 MS markers. The results revealed that Berkshire breeds have excellent meat quality, compared with the popular domestic breeds such as Duroc, Yorkshire, and Landrace. Noticeably, the Berkshire breeds exhibited a significant post-mortem pH24hr ($5.88{\pm}0.01$) and fat content ($2.878{\pm}0.06$). Through the linkage analysis between MS markers, 14 MS markers showed significant association with meat quality traits (p<0.05). Maximum significant differences of 0.55 pH24hr value and 2.04% fat content were observed between the highest and lowest allele populations. If these 14 MS markers are applied to the pork quality diagnosis kit, the synergistic effect can be expected in meat quality parameters such as meat color, fat content, pH 24 hr, cooking loss, drip loss and water-holding capacity.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.05a
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• pp.239-242
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• 2005

본 연구는 성장 속도 및 서로 다른 육질 특성을 지닌 돼지 품종을 이용하여, 육질 및 성장에 관련된 유전자원을 확보하고, 이를 이용한 유전 육종의 기초 자료를 제공하기 위하여 수행하였다. Differential display (DD) RT-PCR 기법을 통해 돼지 품종 간 발현 차이를 보이는 유전자인 NADH dehydrogenase 1과 ATPase 6를 동정하였다. 동정된 유전자의 발현량 분석을 위한 RT-PCR 결과, 각 유전자의 발현량이 재래돼지에서 외래 품종 (랜드레이 스 및 요크셔)에 비해 2배 이상 높음을 확인 할 수 있었다 (p<0.01). 이러한 발현차이 유전자를 이용하여 육질과의 관련성 연구 및 유전자의 기능에 대한 연구가 지속되어야 할 것이다.