Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Development of High Meat Quality Using Microsatellite Markers in Berkshire Pigs

Microsatellite Marker를 이용한 육질 우수 버크셔 계통 조성에 관한 연구

  • Lee, Yong-Hwa (Swine Science and Technology Center, Gyeong Nam National University of Science and Technology) ;
  • Kwon, Seul-Gi (Swine Science and Technology Center, Gyeong Nam National University of Science and Technology) ;
  • Park, Da-Hye (Swine Science and Technology Center, Gyeong Nam National University of Science and Technology) ;
  • Kwon, Eun-Jung (Swine Science and Technology Center, Gyeong Nam National University of Science and Technology) ;
  • Cho, Eun-Seok (Swine Science and Technology Center, Gyeong Nam National University of Science and Technology) ;
  • Bang, Woo-Young (Swine Science and Technology Center, Gyeong Nam National University of Science and Technology) ;
  • Park, Hwa-Chun (Pig Breeding Company) ;
  • Park, Beom-Young (National Institute of Animal Science, RDA) ;
  • Choi, Jong-Soon (Korea Basic Science Institute) ;
  • Kim, Chul-Wook (Swine Science and Technology Center, Gyeong Nam National University of Science and Technology)
  • 이용화 (경남과학기술대학교 양돈과학기술센터) ;
  • 권슬기 (경남과학기술대학교 양돈과학기술센터) ;
  • 박다혜 (경남과학기술대학교 양돈과학기술센터) ;
  • 권은정 (경남과학기술대학교 양돈과학기술센터) ;
  • 조은석 (경남과학기술대학교 양돈과학기술센터) ;
  • 방우영 (경남과학기술대학교 양돈과학기술센터) ;
  • 박화춘 (다산종돈) ;
  • 박범영 (농촌진흥청 국립축산과학원) ;
  • 최종순 (한국기초과학지원연구원) ;
  • 김철욱 (경남과학기술대학교 양돈과학기술센터)
  • Received : 2010.10.27
  • Accepted : 2011.03.25
  • Published : 2011.04.30
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Abstract

In this study, the efficiency of microsatellite (MS) markers for pork quality was examined and further, their suitability to domestic pork industry also was verified, by measuring meat quality parameters of Berkshire breeds. A total of 323 pigs of Berkshire breeds were slaughtered and subjected to meat quality evaluation. In addition, the genomic DNAs from blood samples of slaughtered pigs were used for genotyping analysis of 50 MS markers. The results revealed that Berkshire breeds have excellent meat quality, compared with the popular domestic breeds such as Duroc, Yorkshire, and Landrace. Noticeably, the Berkshire breeds exhibited a significant post-mortem pH24hr ($5.88{\pm}0.01$) and fat content ($2.878{\pm}0.06$). Through the linkage analysis between MS markers, 14 MS markers showed significant association with meat quality traits (p<0.05). Maximum significant differences of 0.55 pH24hr value and 2.04% fat content were observed between the highest and lowest allele populations. If these 14 MS markers are applied to the pork quality diagnosis kit, the synergistic effect can be expected in meat quality parameters such as meat color, fat content, pH 24 hr, cooking loss, drip loss and water-holding capacity.

본 연구는 버크셔종의 육질에 대한 특성을 분석하여 국내 돈육시장에서의 적합성을 확인하고, MS marker를 이용한 육질 우수 개체 선발의 효율성에 대해서 분석하였다. 버크셔 323두를 동일 사양조건에서 사육하고 도축하여 육질형질을 분석하고, 혈액으로부터 genomic DNA를 분리하여 50개의 MS marker에 대한 유전자형을 분석하였다. 버크셔종은 국내에서 가장 많이 이용되고 있는 듀록, 요크셔, 랜드레이스 종보다도 육질에 있어서 더욱 우수한 특성을 나타내었다. 특히 도축 24시간 후 pH 값은 평균 $5.88{\pm}0.01$로 대단히 높게 나타났고, 지방함량의 경우에도 $2.878{\pm}0.06$로 확인되었다. MS marker와의 연관성을 확인한 결과, 14개 MS marker가 육질형질과 연관성을 있는 것으로 나타났다(p<0.05). 특히 pH24hr와 지방함량에서는 가장 높은 값을 가지는 대립유전자집단이 가장 낮은 집단에 비해 최고 0.55, 2.04%가 높은 것을 확인할 수 있었다. 이러한 특성을 버크셔종의 육질 우수 개체 선발에 이용한다면 육색, 지방함량, pH24hr, 가열감량, 드립감량, 그리고 보수성에서 육질 형질값의 높은 개량효과를 기대할 수 있을 것으로 판단된다.

Keywords

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