• The Korean Journal of Zoology
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• v.35 no.3
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• pp.362-371
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• 1992

Spiromeiru erinocer익 유충(sparganum)을 전이숙주(paratenic host)인 흰쥐와 종숙주인 고양이에 감염시켜 발육단계에 따라 회수해서 유충과 성충조직의 LDH(lactate dehydrogenase)와 SDH(succinate dehydrogenase)의 분포상 및 동위효소 유형을 효소조직화학적 방법과 전기영동법으로 조사하였다. 첫째, 유충조직에서의 LDH분포는 발육과정에 따른 차이는 없었으며 표피층과 실질근층에 비해 표피근층이 많이 분포하였다. 성체조직에서도 표피근층에 많이 분포하였는데 특히 성체의 표피층에서의 LOH 활성은 수태편절에서 상당히 많은 활성을 보인 반면 다른 편절의 표피층에서는 LDH의 활성이 없었다. 둘째, SDH의 조직분포는 유충과 성충 모두 표피근층에 많이 분포하58h으며 유충에서의 발육 후기는 발육초기보다 SDH의 분포량이 증가하였다. 셋째, LDH등위효소 유형은 유충과 성충에서 2개씩 나타났는데 유충에서는 M.W. 140 Kd가, 성충에서는 56 Kd가 주분획(major band)이었다. 넷째, SDH등위효소는 유충에서 1개(132 Kd), 성충에서 2개(132 Kd, 45 Kd)가'분리되었으며 성충에서 활성이 높게 나타난 band는 45 Kd인 단백질이었다.

• Korean journal of applied entomology
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• v.36 no.1
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• pp.48-54
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• 1997

This study was conducted to determine the effect of temperatures and food sources on the egg and larval developmentof the tobacco cutworm, Spodoptera litura Fabricius. The hatchability of egg masses of S. liturawas 100% on the leaf of soybean, perilla and sweet potato in any given temperature regimes, while the hatchabilitywas only 65-8796 when reared on the pulp paper and decreased as temperature increased. Egg durationwas not significantly different among different food sources within each temperature. However, egg duration at32$^{\circ}$C was shorter than that at 24$^{\circ}$C and 28$^{\circ}$C. During the early larval development, at 28$^{\circ}$C and 32$^{\circ}$C the larvafed on sweet potato leaf was heavier than those fed on soybean and perilla leaves and the opposite case wastrue during mid-larval development stage. However, larval weight at 24$^{\circ}$C was heavier on sweet potato leafthan that on soybean and perilla leaves until 12 days after hatching. This result was probably due to relativelyslower developmental rate at 24$^{\circ}$C compared to 28$^{\circ}$C and 32$^{\circ}$C. The mean larval mortality was 68.896, 44.5%and 33.8% at 24$^{\circ}$C. 28$^{\circ}$C and 32"C, respectively. The lowest mortality was observed on soybena leaf and followedby perilla and sweet potato leaves, and artificial diet regardless of temperature conditions. The durationwas the shortest when they fed on soybean leaf, and followed by perilla and sweet potato leaves and artificialdiet. Larval durations were 23.6-30.4 days at 24$^{\circ}$C. 18.6-22.3 days at 28$^{\circ}$C and 14.5-18.0 days at 32$^{\circ}$C. Thethreshold temperatures of egg and larva of S. litura were about 6.l"C and 10.9"C, respectively.t 6.l"C and 10.9"C, respectively.pectively.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.387-394
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• 2013

The developmental times of mealworm beetle larvae, Tenebrio molitor were studied at six temperatures ranging from 15 to $30^{\circ}C$ with 60~70% RH, and a photoperiod of 14L:10D. Mortality of larval period was very low at 17 and $20^{\circ}C$ but did not die over $22^{\circ}C$. Developmental time of larva was decreased with increasing temperature. The total developmental time of T. molitor larvae was longest at $17^{\circ}C$ (244.3 days) and shortest at $30^{\circ}C$ (110.8 days). Egg and larvae were not developed at $15^{\circ}C$. The lower developmental threshold and effective accumulative temperatures for the total larval stages were $6.0^{\circ}C$ and 2564.1 degree-days, respectively. The relationship between developmental rate and temperature was fitted by a linear model and nonlinear model of Logan-6($r^2$=0.95). The distribution of completion of each development stage was well described by the 2-parameter Weibull function ($r^2$=0.8502~0.9390).

• Korean journal of applied entomology
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• v.38 no.3
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• pp.217-224
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• 1999

This study was conducted to determine the larval development of tobacco cutworm, Spdodoptera litura Fabricius, reared on leaves of different leguminous plants of 11 varieties or cultivars, and to measure amount of leaves fed by the larva. Larval duration ranged from 11.5 to 15.7 days depending on different food with the shortest on geomjeongkong-1 and the longest on daek-wangddangkong. Among 6 larval development stages, the 1st instar stages was the longest(3.2~5.0 days) while the 4th instar was the shortest (1.0~1.5 days). In general, amount of leaves consumed was increased with larval age, and consumed from 5 to 74% of total food only during the last instar stage. And female consumed more food than male. While, larval mortality and the sex-ratio seem to have no relation with the amount of food per species.

• Korean journal of applied entomology
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• v.30 no.2
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• pp.130-130
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• 1991

현미를 사료로하여 네가지 정온도조정에서(25, 28, 34, 36$^{circ}C$ $pm$0.5$^{\circ}C$, RH 70%)에서 거짓쌀도둑(Tribilium castaneum Herbst)의 각 충태별 평균발육기간($\pm$SE)을 산출하였으며, 이를 기초로하여 발육임계온도를 추정하였다. 난의 평균발육기간은 각 온도에서 7.6$\pm$0.25, 4.8$\pm$0.10, 3.0$\pm$0.03, 2.5$\pm$0.09일, 유충에서 각각 53.3$\pm$1.49, 33.4$\pm$0.57, 30.6$\pm$0.70, 31.0$\pm$1.18일, 용에서 각각 12.1$\pm$0.17, 7.8$\pm$0.09, 5.0$\pm$0.07일로 산출되었으며, 난에서 성충우화시기까지의 전발육기간은 각각72.3$\pm$1.67, 46.0$\pm$0.05, 39.4$\pm$0.64, 38.7$\pm$1.15일로 산출되었다. 난과 용의 발육기간은 밀과 옥수수같은 다른 식이조건에서와 유사하였으나, 섭식을 하는 유충기의 발육은 밀보다 현미에서 상대적으로 지체되는 현상을 보였다. 한편 현미를 섭취했을 경우, 밀을 섭취했을 때 보다 발육중 치사율이 높아 밀에 비해 현미가 저급의 식물임을 보여주었다. 거짓쌀도둑 난, 유충 용 및 전발육기간의 저온발육임게온도는 각 충태 공히 20.0$^{\circ}C$로 산출되었으며, 유충과 전발육기간의 고온발육임계온도는 각각 40.2$^{\circ}C$와 41.9$^{\circ}C$로 추정되었다. 영기수는 온도에 상관없이 최빈치 7회를 나타냈지만, 온도의 상승에 따라 7회 이상 탈피하는 비율이 높아지는 경향을 보였다. 성비는 온도와 무관한 것으로 나타났다.

• Korean journal of applied entomology
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• v.28 no.3
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• pp.113-119
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• 1989

Larval development and adult oviposition of the Oriental tobacco budworm, Heliothis assulta were examined with tobacco leaves sprayed with aqueous or ethanolic extracts from ten selected wild plant species. High larval mortality was observed with extracts from Rhamnus davurice. Persicaria hydroPiPer, Forsythia koreana, Trifolium repens, Styrax japonica, Ginkgo bi/oba, and vilis amurensis. Most of larval mortality occurred during first and second instar, apparently due to antifeeding effects of plant extracts. These extracts also prolonged developmental period of survived larvae and increased the number of larval molts. Oviposition was not affected as much as the larval mortality, but Rhamnus davurice and Styrax jaPonica extracts reduced the number of eggs laid by more than fifty percent.

• Parasites, Hosts and Diseases
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• v.32 no.4
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• pp.277-280
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• 1994

In the definitive hosts, metacercariae of Parofonimn westermani excyst in host duodenum, penetrate intestinal wall, migrate peritoneal and thoracic cavities, and develop to sexual maturity in 8 weeks. This study was undertaken to examine the age of the maturing p. westernnni when their infectivity to the other definitive hosts was retained. On 3, 7, 10, 14,21 and 28 days after feeding the metacercariae to cats through a gastric tube, the developing worms were harvested. The juveniles of different age were fed again to other experimental cats. One to 12 weeks after the oral-transfer infections, the experimental cats were examined for establishment of infections. In the cats to which 3- day and 7-day old juveniles (grown up to 1.4 mm long) were fed, 31.4% and 22.6% of the transferred worms were found infected. The worms of 10-28 days old were not infective. Early maturing stages grown up to 7 days maintained their infectivity to the other definitive hosts.

• Journal of Sericultural and Entomological Science
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• v.35 no.1
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• pp.28-35
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• 1993

Treatment of juvenile hormone analogue(JHA) at the 5th instar larvae prolonged the duration of adult development one to one and half days as well as the elongation of feeding time with the increasing of larval body weight. Morphological observation and protein analysis in hemolymphs, integuements, alimentary canals, fat bodies and ovaries also revealed that the development of these tissues and organs for adultation are affected by the JHA treatment.

• The Korean Journal of Soil Zoology
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• v.5 no.2
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• pp.119-123
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• 2000

Effect of fluctuating temperatures on the development of the immature beet armyworm, Spodoptera exigua (H bner), was analyzed. At constant rearing temperature regimes, the estimated developmental threshold temperatures were varied among stages and instars, but had an average 13$^{\circ}C$ from egg hatch to adult emergence. Based on the 13$^{\circ}C$ threshold temperature, we set up three different rearing temperature regimes having the same day-degrees. Two fluctuating temperature regimes changed significantly the developmenta1 period expected by the constant rearing temperature regime. Under the same thermophase temperature (25$^{\circ}C$), the thermocycling regime with the higher cryophase temperature (10$^{\circ}C$) decelerated the developmental rate probably by lowering temperature limit thor development, but that with the lower cryophase temperature (5$^{\circ}C$) gave a negative developmental effect.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.1-7
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• 2003

Morphological characteristics, developmental period, and seasonal occurrence of Adeia leucomelas (L.) were investigated from 1999 to 2000. In addition, consumption of sweetpotato as food was also examined. Adults of A. leucomelas were dark-brown and body lengths of females and males were 20.2 mm and 18.9 mm, respectively, Wing expanse of female and male was 33.7 mm and 29.4mm, respectively. Egg was flat round-shape. Larva was light yellow-green to dark-brown with 3.3-53.5 mm. Pupa was deep-brown and 15.1 mm in length. Developmental periods of A. leucomelas from egg to adult emergence at different temperatures of 15, 20, 25, and 30$^{\circ}$C were 108.5, 70.7, 40.2, and 29.1 days, respectively, Developmental threshold (DT) and effective accumulative temperatures were estimated as 10.7$^{\circ}$C and 67.5 DD in egg stage, 11.0$^{\circ}$C and 275.1 DD in larval stage and 9.3$^{\circ}$C and 244.6 DD in pupal stage, respectively. The longevity of adult female was shortened with increment of temperature, whereas the total numbers of eggs laid by a female were increased. The larvae of A.leucomelas occurred from mid-June to early October, and population reached its peak during early to mid-September in Jeonbuk province. Food consumption of A. leucomelas was highest at 20-25$^{\circ}$C. Food consumption of 1 st, 2nd, 3rd, 4th, and 5th larvae of A. leucomelas per day at 25$^{\circ}$C was 0.4, 3.6, 19.6, 40.7, and 78.9 $\textrm{cm}^2$, respectively.