• Korean Journal of Plant Taxonomy
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• v.40 no.2
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• pp.118-129
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• 2010

A taxonomic study of Viola albida complex, containing the representative individuals of three taxa, V. albida var. albida, V. albida var. chaerophylloides, and V. albida var. takahashii, was done based on RAPD data. The amplified loci were 476 in total; obtained with 68 universal primers on seven OTUs. Nei's genetic dissimilarity appeared relatively low within individuals of V. albida var. albida and V. albida var. chaerophylloides (0.118-0.171 and 0.051 respectively), however, it was higher in individuals of V. albida var. takahashii (0.348). On the other hand, there is no specific trend in terms of genetic dissimilartiy among taxa, such as between individuals of V. albida var. albida and V. albida var. takahashii, between those of V. albida var. albida and V. albida var. chaerophylloides, and between those of V. albida var. albida and V. albida var. takahashii. The similarity of OTUs studied is high in clustering analysis, so that this result is compatible with the establishment of this complex. All OTUs are clustered within two groups. The individuals of V. albida var. takahashii, however, are clustered both to the group of V. albida var. albida and to the group of V. albida var. chaerophylloides, meaning that the genetic difference is high which would be commensurate with their morphological variations.

• Animal Systematics, Evolution and Diversity
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• v.12 no.2
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• pp.121-136
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• 1996

In order to understand affinities and phylogeny of three families of the Superfamily Entomobryoidea allozyme analysis was performed with three species presumably representing each family, in addition to four species belonging to the neighboring Superfamily Poduroidea together. Electrophoresis for this purpose produced data for calculating allele frequency and enabled to obtain genetic distance and to depict dendrograms as well. The species of Isotomidae, Entomobryidae and Tomoceridae were clustered together whereas those of Hypogastruridae, Onychiuridae and Neanuridae were grouped as monophylies, respectively. Even though Tomoceridae and Entomobryidae were brought together they are considered complete families by showing high genetic distance value between them, thus supporting the hypothesis on their affinity among others. Gulgastrura reticulosa has been revealed to be separated from Hypogastruridae by being clustered rather with Onychiuridae. However, its high genetic distance value suggests the status as an independent family. The present result agreed with the phylogeny of Suborder Arthropleona divided into two Superfamilies mentioned above as in conventional systematics and also when compared with analysis of data of their morphological characters as well as 18S rDNA performed and published elsewhere by the present writers.

• Korean Journal of Plant Taxonomy
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• v.34 no.1
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• pp.43-61
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• 2004

Molecular taxonomic studies were conducted to evaluate interspecific relationships in Korean Viola 34 taxa including two Japanese populations using RAPD(randornly amplified polymorphic DNA), ISSR(inter simple sequence repeat) and PCR-RFLP(restriction fragment length polymorphism) analysis. Only six and four primers out of 40 arbitrary and 12 ISSR primers were screened for 34 taxa, and were revealed 70 (98.6%) and 28 (96.6%) polymorphic bands, respectively. Fifteen restriction endonucleases produced 80 restriction sites and size variations from the large single copy region of cpDNA, 16 (20%) of which were polymorphic. The separate analyses from the RAPD, ISSR and PCR-RFLP data were incongruent in the relationships among 34 taxa, but combined data was in accordance with previous infrageneric classification system based on morphological characters, especially the subsection and series level. Section Chamaemelanium placed between subsect. Patellares and Vagimtae of section Nomimium was not formed as a distinct group. Viola alb ida complex including three very closely related taxa was recognized independent group within subsect. Patellares in combined data tree. This result strongly suggested that they should be treated to series Pinmtae. RAPD analysis was very useful to clarify the interspecific relationships among the species of Korean Viola than ISSH and PCR-RFLP analyses.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.3
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• pp.220-227
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• 1995

An underwater speaker was designed and used as sound source for thronging shoal of squid in squid angling gear operation. The frequency characteristics of the designed speaker was analyzed experimentally and the thronging response of shoals of squid which may be a key parameter for a new sound catching method, was characterized in audible frequency. The field experiment was carried out in the coast of Cheju Island. The results of this study are summarized as follows; 1. Amplitude response of the speaker shows a maximum in their the frequency of 500Hz. 2. The output waveform distortion is not measured in the frequency range of 250~600Hz. 3. A underwater noise of shoals of squid which were thronged by fish lamp in night appeared the center frequency of 300~400Hz. 4. The shoals of squid shows a thronging response, when a manufactured underwater speaker transmits a intermittent audible sound of 300~400Hz in 10m depth of water.

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.509-514
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• 2006

For the variant identification of Korean dogwood, Cornus kousa Buerg., we investigated useful genetic marker using the ISSR primer. The overall amplicons was 58 in six primers and the mean number of amplicons per primer was 9.67. The UPGMA dendrogram based on the genetic distance showed two major clusters composed of pink-bracted and white-bracted groups respectively. And white-bracted group was divided to two groups, island and inland groups. This result showed that ISSR analysis provides useful markers for the variant identification even in the seedling or young tree of Cornus kousa, especially pink-bracted individuals of Isl. Oenaro.

• Journal of Plant Biology
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• v.37 no.1
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• pp.111-124
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• 1994

The delimitation and systematic relationships of the C. foetida complex and related species were evaluated by numerical methods, including principal components analysis and cluster analysis of major morphological characters. Results of these analyses strongly support recognition of three varieties in C. foetida; these include C. foetida var. foetida, C. foetida var. micrantha, and C. foetida var. velutita. The results also indicated that C. brachycarpa, C. frigida, C. heracleifolia, and C. yunnanensis are distinct species, and C. heracleifolia comprises C. heracleifolia var. heracleifolia and C heracleifolia var. bifida. Cimicifuga mairei and C. foetida var. foliolosa, however, appeared to be morphologically indistinguishable from C. foetida var. velutina. In addition, C. foetida var. longibracteata and C. foetida var. bifula are conspecific with C. frigida.rigida.

• Korean Journal of Environment and Ecology
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• v.24 no.4
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• pp.493-499
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• 2010

To obtain the basic data for the conservation and cultivation of major native herb species under the trees, distribution of native herb species, and corrrlations between native herb species and site factors were studied on natural broadleaved forest in Mt. Gariwang and Mt. Jungwang, Pyeunchang-gun. 136 native herb species were distributed in studied area, and the importance percentage of Pseudostellaria heterophylla was proved the highest, 10.8%. The other major herb species were the following oders, Meehania urticifolia, Corydalis remota, Pimpinella brachycarpa, Carex siderosticta, Pseudostellaria palibiniana, Erythronium japonicum, Ligularia fischeri, Asarum heterotropoides var. mandshuricum, Hylomecon vernalis and Astilbe rubra var. rubra. Highly positive correlations were proved Hepatica asiatica and Ainsliaea acerifolia, Pimpinella brachycarpa and Aconitum jaluense subsp. jaluense, Aconitum jaluense subsp. jaluense and Smilacina japonica var. japonica, Meehania urticifolia and Pseudostellaria palibiniana. Seven groups of native herb species were divided by cluster analysis. Preferring site factors for each native herb species were determined. Ligularia fischeri, Ainsliaea acerifolia, Parasenecio auriculata var. matsumurana, Erythronium japonicum and Pimpinella brachycarpa were distributed mainly on the higher sites, Ligularia fischeri were ditributed more frequently in deep soil sites. These results might be useful measures in the conservation and cultivation of native edible and medicinal herb species on the forest floor in the natual broadleaved forest, Pyeungchang-gun.

• Korean Journal of Plant Taxonomy
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• v.42 no.3
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• pp.215-221
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• 2012

Five Korean Calanthe species, C. discolor, C. bicolor, C. sieboldii, C. reflexa, and C. aristulifera, were studied using amplified fragment length polymorphism (AFLP) to assess their taxonomic and genetic relationships. Sixteen accessions belonging to five native Calanthe spp. and mutants with yellow tepal and white lip (YW mutants) were studied. We identified 50 putative markers using AFLP analysis. The results of AMOVA showed that genetic variance was higher between species than within species. Genetic dissimilarity when compared with the rest of the species was the lowest for individuals of the YW mutants and the highest for individuals of C. reflexa. The mutants clustered outside the major group. Calanthe bicolor clustered with C. discolor, suggesting that its genetic composition is closer to that of C. discolor. Though it is suggested to have originated as a result of natural hybridization between C. sieboldii and C. discolor, introgression is likely to have occurred in the direction of C. discolor based on the data of molecular marker, clustering and genetic dissimilarity. Calanthe reflexa and C. aristulifera were genetically the most diverse of the species studied. In conclusion, the results showed that there is genetic diversity in Korean Calanthe species, that C. bicolor introgressed in the direction of C. discolor and that the YW mutants are genetically closer to C. sieboldii.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.150-160
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• 2006

This study was performed to provide basic information of the development and breeding of new cultivars of Hibiscus syriacus L., which have more beautiful and diverse flowers. Morphological characteristics of the leaves and genetic variation of the leaves of two Jeoktanshim-line cultivars, Bulsae and Pyungsung, were crossed each other. The result of the cross between Bulsae and Pyungsung are as follows: Mean leaf length and width were 6.09cm and 3.36cm, respectively. Mean petiole length was 1.08cm, and mean shoulder width and incision depth were 1.50cm and 0.84cm, respectively. Mean leaf base angle was $98.56^{\circ}$. Leaf index and petiole length were the most important components of leaf characteristics. Two groups were clustered in distance level 5.4. The result of the cross between Pyungsung and Bulsae are as follows: Mean leaf length and width were 5.25cm and 3.10cm, respectively. Mean petiole length was 0.87cm, and mean shoulder width and incision depth were 1.50cm and 0.76cm, respectively. Mean leaf base angle was $107.87^{\circ}$, which was the widest among ten cross combinations. Two groups were clustered with seven leaf characteristics in distance level 4.2. Group I included two small groups, and group II included four small groups.

• Korean Journal of Plant Resources
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• v.18 no.2
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• pp.260-269
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• 2005

Taxonomic studies on morphological, principal component analysis (PCA), palynological, RAPD and PCR-RELP analysis were conducted to intraspecific relationships of Rubus oldhami. Three types of Rubus oldhami based on the flower characters such as petal length and number were used in this study. Among the 14 morphological characters, perianth length, calyx lobe length, apical leaflet shape and leaflet length were used to distinguish for each type. The pollen characters such as shape, aperture number, surface sculpture were showed very similar among three types. Eight primers out of 20 arbitrary primers were screened for three types, and were revealed 33 ($60\%$) polymorphic bands. The phonogram by RAPD data showed incongruent with morphological analysis. Even though ten restriction endonucleases produced 20 restriction sites, polymorphic bands were not observed. Based on the results, three types of Rubus oldhami divided well by morphological characters, but pollen and DNA data were not supported. Therefore, type 1 and 2 which different from type 3 by flower characters considered as a temporary hybrid or ecotype because of their similar habitats.