• The Microorganisms and Industry
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• v.20 no.1
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• pp.2-9
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• 1994

최근에 미국의 국립보건원과 에너지성에서는 1990년부터 합동으로 수행하고 있는 게놈연구 5개년 계획(1991-1995)을 보완하여 새로운 5개년(1994-1998) 계획을 마련하였다. 이 계획에는 인체게놈의 지도작성 및 염기서열결정, 물리지도에 알려진 유전자들의 위치결정, 게놈연구에 필요한 기술, 모델 organism, 정보의 축적, 윤리, 법 및 사회적인 문제, 게놈연구에 관련된 교육훈련, 기술이전, 축적된 기술 및 데이타의 상호교환 등에 관한 구체적인 계획들의 변경을 포함하고 있다. 본문에서는 인체게놈연구 수행을 위하여 필요로 하는 몇몇 게놈연구 관련 기술과 현재 이용되고 있는 유전자지도 작성방법 등에 대하여 알아보고 향후의 게놈연구 방향에 관하여 기술하려고 한다.

• The Korea Swine Journal
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• v.16 no.7 s.179
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• pp.73-76
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• 1994

• The Korea Swine Journal
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• v.16 no.6 s.178
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• pp.130-133
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• 1994

• The Science & Technology
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• v.30 no.10 s.341
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• pp.82-83
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• 1997

콩의 유전자지도 작성으로 고품질의 새로운 품종개발에 심혈을 기울이고 있는 농촌진흥청 전작과 농업연구사 이석하박사. 우리나라가 콩의 고향이라고 말하는 이박사는 유전자지도를 더욱 세밀화하여 앞으로 나물콩, 밥밑콩, 두부용콩, 비린내 없는 콩 등의 품종개발에도 연구를 계속하고 있다.

• Proceedings of the Korean Society of Crop Science Conference
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• 1996.05a
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• pp.26-27
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• 1996

• The Korea Swine Journal
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• v.16 no.10 s.182
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• pp.137-139
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• 1994

• Horticultural Science & Technology
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• v.28 no.4
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• pp.664-671
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• 2010

A genetic linkage map was constructed using 188 $F_9$ RILs derived from a cross between $Solanum$ $lycopersicum$ H7996 (resistant to bacterial wilt) and $S.$ $pimpinellifolium$ WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at ${\leq}$ 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.473-482
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• 2013

Molecular markers, as an efficient selection tool, have been and is being used for practical breeding program in chili pepper (Capsicum annuum L.). Recently, a lot of researches on inheritance and genetic analysis for quantitative traits including capsaicinoids, carotenoids, and sugar content in pepper are being performed worldwide. It has been also reported that QTL mapping is a necessary tool to develop molecular markers associated with the quantitative traits. In this study, we suggested a new method to construct a pepper genetic map using SNP (HRM) markers generated from NGS resequencing of female and male parents. Plant materials were C. annuum 'NB1' (female parent), C. chinense 'Jolokia' (male parent), and their $F_2$ population consisting of 94 progenies. Sequences of 4.6 Gbp and 6.2 Gbp were obtained from NGS resequencing of 'NB1' and 'Jolokia', respectively. Totally, 4.29 million SNPs between 'NB1' and 'Jolokia' were detected and the 1.76 million SNPs were clearly identified. Among them, total 145 SNP (HRM) primer pairs covering pepper genetic map were selected, and the 116 SNP (HRM) markers of them were located on this map. Total distance of the map, which consisted of 12 linkage groups and matched with basic chromosome numbers of pepper, was 1,167.9 cM. According to the mapping result, we concluded that our mapping method was suitable to construct a pepper genetic map fast and accurately. In addition, the genetic map could be directly used for QTL analysis of traits different between both parents.

• Journal of Plant Biotechnology
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• v.39 no.1
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• pp.33-48
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• 2012

As a scientific curiosity to understand the structure and the function of crops and experimental efforts to apply it to plant breeding, genetic maps have been constructed in various crops. Especially, in the case of Brassica crop, genetic mapping has been accelerated since genetic information of model plant $Arabidopsis$ was available. As a result, the whole $B.$ $rapa$ genome (A genome) sequencing has recently been done. The genome sequences offer opportunities to develop molecular markers for genetic analysis in $Brassica$ crops. RFLP markers are widely used as the basis for genetic map construction, but detection system is inefficiency. The technical efficiency and analysis speed of the PCR-based markers become more preferable for many form of $Brassica$ genome study. The massive sequence informative markers such as SSR, SNP and InDels are also available to increase the density of markers for high-resolution genetic analysis. The high density maps are invaluable resources for QTLs analysis, marker assisted selection (MAS), map-based cloning and comparative analysis within $Brassica$ as well as related crop species. Additionally, the advents of new technology, next-generation technique, have served as a momentum for molecular breeding. Here we summarize genetic and genomic resources and suggest their applications for the molecular breeding in $Brassica$ crop.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.61-61
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• 1993

사람의 혈액의 백혈구로부터 Polymerase Chain Reaction 방법으로 $\beta$$_2$-adrenergic receptor 유전자를 증폭하였다. 이 증폭된 유전자를 pbluescript KS(＋)에 cloning하였으며, 제한효소지도 작성과 부분적인 염기배열 확인으로 증폭된 유전자가 사람의 $\beta$$_2$-adrenergic receptor 유전자임을 확인하였다. $\beta$$_2$-adrenergic receptor 유전자를 N말단의 아미노산이 21개 제거되도록 결손시킨후 yeast의 pSec5의 Killer toxin signal sequence의 뒤에 in-frame으로 연결하였다. 현재 효모에서의 발현양상 및 발현된 단백질의 활성을 조사중이다.