• Title/Summary/Keyword: 유전자변형생물체

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Allergenicity assessment of novel proteins expressed in genetically modified organisms (유전자변형생물체의 알레르기성 평가와 이해)

  • Lee, Sang-Gu;Oh, Seon-Woo;Park, Soo-Yun;Park, Hyoun-Min;Kim, Eun-Ha;Jin, So-Ra;Ryu, Tae-Hun
    • Journal of Plant Biotechnology
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    • v.48 no.4
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    • pp.201-206
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    • 2021
  • To ensure the safety of developing or importing genetically modified organisms (GMOs), Korea has enacted the "LMO Act." Accordingly, the safety of using GMOs as food or feed is evaluated in accordance with the concept of "substantial equivalence" proposed by OECD. The allergenicity of GMOs is assessed as a part of their safety evaluation. The methods of allergenicity assessment have been discussed by various international organizations, such as the OECD, FAO, and WHO. The main methods used for the allergenicity assessment of proteins newly expressed in GMOs include assessment of the physicochemical stability of these proteins, evaluation of their amino acid homology with existing allergenic proteins, and serum screening. In this study, we describe guidelines and related studies for the allergenicity assessment of GM crops.

Selection of transgenic Solanum nigrum L. used environmental remediation expressing organomercurial lyase (Organomercurial lyase 유전자를 도입한 환경정화용 형질전환 까마중(Solanum nigrum) 선발)

  • Choi, Kyung-Hwa;Kim, Yong-Ho;Chung, Hyen-Mi;Choi, Young-Im;Noh, Eun-Woon;Kim, Hyun-Soon;Jeon, Jae-Heung
    • Journal of Plant Biotechnology
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    • v.35 no.4
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    • pp.291-298
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    • 2008
  • Methylmercury, an organic derivative, is the principal form of mercury that biomagnifies and causes neurodegenerative symptoms in animals. In recent years, living modified organism (LMO) resulting from biotechnology has played a highly visible and controversial role. Despite the potential benefits of this technology, public concerns have been raised about the environmental risk of LMO. The concern on the risk from LMO release has urged efforts to evaluate and manage the risks of the LMO. To build up the capacity building of risk assessment method for LMO used environmental remediation, we engineered Solanum nigrum L, expressing the modified bacterial gene, merB, encoding organomercurial lyase. Two independently isolated transgenic lines produced merB RNA. Transgenic Solanum nigrum leaf discs expressing merB gene showed organic mercury resistance, forming shoots well on growth medium containing $0.5{\mu}M$ methylmercury (II) chloride and $1{\mu}M$ phenylmercuric acetate while control plants breached. Transgenic merB seeds germinated and grew on growth medium containing $2{\mu}M$ methylmercury (II) chloride and phenylmercuric acetate. The merB transgenic plants will be used for risk assessment of natural environment.

A Study on Activation Strategy of Biosafety Training for LMO Research Safety Management (시험·연구용 유전자변형생물체(LMO) 안전관리를 위한 안전교육 활성화 방안)

  • Rho, Young Hee;Min, Wan Kee;Jeong, Gyu Jin
    • Journal of the Korean Society of Safety
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    • v.29 no.2
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    • pp.98-105
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    • 2014
  • Biosafety has become quite sensitive issues according to dramatic development of biotechnology and LMO(Living Modifying Organism) is one of the key issue in biosafety. This study is an exploratory research for investigating the activation strategy of biosafety training management in LMO research field. Based on the survey data, main results are derived through various statistical analysis methodology such as descriptive analysis, factor analysis, reliability analysis, analysis of variance and regression analysis. According to the analysis results, some activation strategies are required to reach the target such as extension of specialized biosafety training program, enhancement of safety consciousness from the undergraduate courses, introduction of appropriate safety regulations, unification of safety management and establishment of safety management system.