• Journal of the Korea Concrete Institute
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• v.23 no.3
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• pp.331-338
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• 2011

The purpose of this study is to use organisms or micro-organism functions for eco-friendly water-purification of cement bricks, utilizing bioremediation. Many researches have been performed in the past to improve water quality by using effective micro-organisms in construction materials. In order to purify water using micro-organisms, this research used soybean paste bacteria, an effective micro-organism that was identified through 16S rDNA sequence analysis performed in Daegu S. Environment Protection Institute in addition to Natto bacteria that was studied in the previous research. With these effective micro-organisms with water-purification ability, this study examined their water-purification possibility on cement bricks. This study used Zeolite to immobilize micro-organisms to bricks, and confirmed that the micro-organisms were attached on Zeolite from SEM analysis. The experimental results showed that specific micro-organisms can be used to effectively remove contamination an used to develop eco-friendly construction materials. The study on micro-organisms for material purification shows great promises as a future research topic.

• Monthly Korean Chicken
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• v.18 no.2
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• pp.97-99
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• 2012

EM 미생물을 육계 사료 내 첨가 급여할 경우 유용미생물군의 영향으로 장내 환경이 안정적으로 바뀌어 생산성 및 환경 역시 개선되게 된다.

• Korean Journal of Microbiology
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• v.49 no.4
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• pp.343-352
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• 2013

In this study, we have evaluated the effect of efficient microorganisms on odor-removal efficiency and early stabilization of the burial sites. We have developed an efficient microorganism designated as 'KEM' which have the ability to degrade organic compounds and remove odor effectively. Other efficient microorganisms already used on site, such as EM and Bacillus sp., were also compared. We preceded these experiment using lab-scale reactors under three conditions (control, only media and only body) and comparing the effect of with or without the application of tree efficient microorganisms separately. Analysis was focused on eight components (ammonia, TMA, $H_2S$, methyl mercaptan, dimethyl sulfide, dimethyl disulfide, $CO_2$ and $CH_4$), and as a result, efficient microorganisms were shown efficiency in the removal of ammonia and methyl mercaptan. The applied KEM decayed up to 71.2% of the buried meat. We were unable to observe significant differences in microbial communities between efficient microorganisms-treated and non-treated reactors due to the large presence of microorganisms in both soil and carcasses. However, it was possible to observe the effect on odor control and decay rate through the application of efficient microorganisms.

• Journal of agriculture & life science
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• v.50 no.1
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• pp.1-22
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• 2016

In this study, the securing state of nationally indicated beneficial microbial resources was evaluated in an aspect of bio-diversity using their taxonomical information. Depending on the analysis result with the Margalef's richness or the Mehinick's index which are representative bio-diversity analytical indices, species diversity values was revealed as 8.537, 3.546 within bacterial resources, 3.349, 2.167 within fungal resources. Several developed or researched beneficial strains and spoilage microbes showed relative taxonomical relationship with comparation of their biological information. As a result, we propose the necessity or countermeasure method for preventing the microbial spoilage with the overhauling consideration of advanced research on agricultural microbiology covering crop endophyte beneficial/spoilage microorganisms.

• The Microorganisms and Industry
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• v.20 no.2
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• pp.33-40
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• 1994

미생물에 대한 유전자 재조합법 등의 개발로 동물세포에서만 합성되던 단백질들을 미생물을 통하여 생산하는 기술이 확립되어 있으나, 동물 세포내에서만 정확하게 실행되어지는 단백질 분자의 folding과 post-translational modification 등이 미생물에서는 불완전하게 이루어져 활성을 잃게 되는 단점이 있고, pyrogen과 같이 미생물로부터 유래한 endotoxin이 생산물에 섞여 있을수도 있으며, 미생물로부터 생산되는 각종 단백질로부터 원하는 유용 단백질을 분리하기 어려운것 등, 현실적으로 많은 어려움을 가지고 있기 때문에 미생물을 이용하기보다 동물 세포 배양을 통하여 위와 같은 제재들을 생산하려 하고 있다. 유전자 재조합 기술은, 현재 미생물뿐만 아니라, 동,식물 세포에 대하여도 적용되어 있어서 각종 유용생산물을 동,식물세포의 유전자 조작을 통해 얻을 수 있는 단계에 와 있으며, 이는 유전자 치료(gene therapy)와 같은 의료분야에까지 확장될 수 있게 되었다. 표 2에서는 동물 세포를 배양할 때와 미생물을 이용할 때의 각각의 특징을 보여주고 있다.

• Journal of the Korea Concrete Institute
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• v.26 no.2
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• pp.219-227
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• 2014

The purpose of this study is to investigate water purification properties of porous concrete by using effective microorganisms through the long-term continuous flow test. To solve the problems such as desorption of conventional microorganisms, in this study, tertiary treatment of the effective microorganisms identified by 16S rDNA sequence analysis was adopted per each step in the manufacturing process of porous concrete. And concentration for optimum continuous flow test and operation conditions through basic experiments according to retention time were investigated. Based on the experimental results, the porous concrete applying effective microorganisms showed no toxicity on the biological water quality and exhibited excellent removal efficiency than normal porous concrete. Therefore, contaminated water quality would be improved by treatment performance investigation of contaminants through long-term continuous flow test. If problems are complemented during the experiment process, it is expected to be able to reduce the non-point pollution sources flowing into river.

• The Microorganisms and Industry
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• v.30 no.1
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• pp.21-29
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• 2004

• The Microorganisms and Industry
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• v.19 no.1
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• pp.59-62
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• 1993

• Journal of the Korea Concrete Institute
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• v.22 no.4
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• pp.547-557
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• 2010

This paper presents a study on the development of next generation smart concrete in an eco-friendly manner using micro-biologically induced calcite precipitation (MICP) via microbial biomineralization. It seems that currently, the reformation and functional improvement of concrete using MICP can be achieved using Sporosarcina pasteurii, which is a representative microorganism that produces calcite precipitation. Based on previous studies on MICP the biochemical tests and crystallinity evaluation of cement using sporoasrcina pasteurii and four additional micro-organisms from the concrete structures as identified by 16S rDNA sequence analysis were conducted. Also by applying the Sporosarcina pasteurii and separated four effective micro-organisms from the concrete structures to mortar, the compressive strength improvement by varying curing conditions, repair of crack were examined, and plans for future study were suggested. The effect of the application of effective micro-organisms can lead to the development of a new material that will contribute to resolution of environmental problems and facilitate repair work, and this can also serve as a new research theme in the future. In addition, the importance of this study is to use micro-organism, which is found common in concrete structures, this new microbial is not only environmentally safe but also persists in the natural environment for an extended period of time. Therefore, it seems to have a great potential to became a new environmentally low-burdened functional material.

• The Microorganisms and Industry
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• v.18 no.3
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• pp.35-40
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• 1992

방선균은 자연계에 널리 분포하고 있고 학문적으로 흥미롭거나 상업적으로 유용한 경우도 많이 알려져 있으나 실제 자연계에 있어서의 자세한 분포 및 역할은 아직 잘 알려져 있지 않은 경우가 많다. 적절한 균 분리원의 선택 및 이로부터의 신규 또는 유용한 방선균만을 효율적으로 분리하는 방법은 아직까지 논의의 여지가 있지만 자연계에는 아직 미지으 유용한 방선균이 많이 존재함에는 이의가 없다. 따라서 목적을 가지고 방선균을 효율적으로 분리하고 이용하는 기술이 향상되면 스크리닝은 성공적이 될 것이다. 이를 위해 본 고에서는 이에 필요한 적절한 균 분리원의 선정, 시료의 전처리, 적절한 배지 등의 선정, 배양조건의 검토 및 효율적인 균 선별 등에 대하여 간략하게 검토하였으며 차후 보다 기본적인 생태학적인 조사 및 분자생물학적인 연구 지원을 통하여 이 분야의 연구는 더욱 발전하리라 보여진다.