• Title/Summary/Keyword: 유생산

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Rotifer 고밀도 반연속 해양에 있어서 담수산 rotifer, Brachionus calyciflorus 와 해수산 rotifer, Brachionus rotundiformis의 생산성

  • 이균우;윤문근;박흠기
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.278-279
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    • 2000
  • 현재, 해수어와 해산 갑각류 종묘생산에 있어 해수 자치어와 유생의 초기먹이로 rotifer, Brachionus plicatilis와 B. rotundiformis를 사용한다. 또한 담수산 rotifer, calyciflorus는 담수산 자치어의 먹이로 이용이 가능하며 특히, 입이 작은 열대 관상어의 초기 먹이로 이용되고 있다. 그러나 rotifer 생산에 있어 일반적인 batch 배양 방법은 많은 공간을 차지하면서 rotifer 생산 기간 중 갑자기 전량 폐사하는 등 배양이 불안정하고 생산성을 고려할 때 양적확보를 위한 생산단가가 비교적 높다. (중략)

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The Complete Larval Development of Enocheir japonicus De Haan (Crustacea, Brachytira, Grapsidae) Reared in the Laboratory (동남참게 Enocheir japonicus의 유생 발생)

  • 김창현;황상구
    • The Korean Journal of Zoology
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    • v.33 no.4
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    • pp.411-427
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    • 1990
  • The complete larval development of Enocheir japonicus De Haan reared in the laboratory is described and illustrated. E. japonicus passes through 5 zoeal stages before metamorphosis to the megalopal stage. The megalopa and crab I inStar are attained in 17 and 27 days after hatching, respectively. The culture was carried out under a photoperiod of 14 h light and 10 h dark and a constant temperature of $25^{\circ}C$. Morphological charaderistics of each larval stage of this species are compared with those of E. japonicus from Japan. Morphological features between zoea I of the Varuninae are briefly discussed.

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The First Zoeal Stage of Pinnotheres sinensis SHEN, 1932 (CURSTACEA,BRACHYRA, PINNOTHERIDAE) Reared in the Laboratory (굴속살이게 (갑각강 .게아목.속살이게과)의 제 1조에아 유생)

  • 고현숙
    • Animal Systematics, Evolution and Diversity
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    • v.7 no.2
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    • pp.257-264
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    • 1991
  • The first zoea of Pinnotheres sinensis showed the characteristics which were correspond well with those of Pinnotheres zoeae ; No carapace spines, endopodites of maxillule and maxilla with 0.4, 1.2(3) setation and endopodite of second maxilliped with 0.4 setation. However, the setations of the basal and coxal endites of the maxillule and maxilla was different from those of Muraoka and Konishi (1977), Konishi(1983).The host of adult crab was Tapes, not mytilus . Thus, there was a need for detailed description of the Korean crab of Pinnotheres sinensis symbiotic with Tapes.

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Effects of Boostin-250 Supplementation on Milk Production and Health of Dairy Cows (재조합 Bovine Somatotropin 250 mg 제제의 투여가 젖소의 산유량 및 건강에 미치는 영향)

  • Kim, Yo-Han;Kim, Doo
    • Journal of Veterinary Clinics
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    • v.29 no.3
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    • pp.213-219
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    • 2012
  • The recombinant bovine somatotropin (rbST) has been used for increasing milk production of dairy cows without adverse health effects. This study was conducted to compare effects of supplementation with $Boostin^{(R)}$-250 containing 250 mg of rbST on milk production with those of $Posilac^{(R)}$ and $Boostin^{(R)}$-S. And safety of rbST supplementation on target animals was also observed. Each twenty-five lactating dairy cows were assigned randomly to one of four groups. $Boostin^{(R)}$-250 and vehicle (control) were administered weekly. $Boostin^{(R)}$-S and $Posilac^{(R)}$ were administered two week intervals. Milk yield, milk components, milk somatic cell count, health status, and body condition score of cows were examined. Supplementation with $Posilac^{(R)}$, $Boostin^{(R)}$-S, and $Boostin^{(R)}$-250 induced more milk yield than control group by 2.9 kg/day (12.3%), 4.2 kg/day (17.9%), and 4.1 kg/day (17.4%), respectively. There was a significant difference in milk yield among three rbST treatment groups and control group (${\alpha}$ = 0.05). The rbST supplementation did not increase the incidence of clinical mastitis and milk somatic cell counts. Supplementation with rbST did not significantly affect milk components (milk fat, protein, and solid not fat). The rbST supplementation of the dairy cows after peak milk yield did not cause negative effect on BCS. However, some cows less than 100 days in milking had decreased BCSs after rbST supplementation. In conclusion, milk production in 250 mg of rbST administered cows every week was similar to that of 500 mg of rbST administered cows every 2 weeks. And supplementation of 250 mg of rbST every week could reduce metabolic stress in cows.

Larval Survival Rate and Growth of Pandalus gracilis Using Two Temperature Conditions Reared in the Laboratory (한국산 남방도화새우, Pandalus gracilis유생의 두 온도 구간에서의 생존율과 성장)

  • 최정화;마채우
    • Journal of Aquaculture
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    • v.17 no.1
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    • pp.58-61
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    • 2004
  • Larvae of Pandalus gracilis were reared in the laboratory under controlled conditions (salinity, 32.5∼34.5; photo-periods, 12L: 12D). The experiments were designed to examine effects of temperature (10 and 2$0^{\circ}C$) on larval development, survival, intermolt period and larval growth. The higher survival rate was obtained at 2$0^{\circ}C$ than at 1$0^{\circ}C$ and larvae reached later stages at 2$0^{\circ}C$. Intermolt period increased significantly with increasing of instar number and was longer at 1$0^{\circ}C$ than at 2$0^{\circ}C$.

Development of Visual System in Korean Salamander(Hynobius leechi) (도룡뇽(Hynobius leechi)의 시각계 발생)

  • Cheong, Seon-Woo;Yoon, Chun-Sik;Kim, Douk-Hoon
    • Journal of Korean Ophthalmic Optics Society
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    • v.3 no.1
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    • pp.9-13
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    • 1998
  • Visual system of Korean Salamander(Hynobius leechi) was morphologically studied. Fertilized eggs in egg sacs were collected and were developed in sterile saline solution. Various sized larvae of 5-30mm in length were fixed. Specimens were paraffin sectioned and were observed under light microscope. In 5mm length larva, lens rudiment induced by optic cup was combined with sensory ectoderm. The shape of lens was changed as spherical in 12mm length larva, but the retinal layer did not differentiated into three layers. The differentiation of retinal layer was clear in 14-16mm length larva. The central region of lens fibers was degenerated. Iris and ciliary body were formed from the marginal zone of optic cup in 20mm length larva. Choroid was thicker in elder eye of 30mm length larva and cartilage developed at outer region of optic cup. The outer segment of photoreceptor cell layer grew longitudinally. Optic nerve was connected to the ventral part of brain through cartilage.

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Effect of Heavy Metals on Larvae of Barnacle, Balanus albicostatus (생태독성 검정을 위 한 고랑따개비 (Balanus albicostatus) 초기유생의 중금속에 대한 민감도 분석)

  • Sung, Chan-Gyoung;Choi, Mi-Sun;Chun, Ho-Hwan;Lee, In-Won;Park, Hyun;Lee, Chang-Hoon
    • Environmental Analysis Health and Toxicology
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    • v.23 no.4
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    • pp.337-343
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    • 2008
  • 따개비류는 암반기질에 무리를 이루어 비교적 단단히 부착생활을 하며 전 세계적으로 분포하는 생물이다. 본 생물은 형태가 다른 두 단계의 플랑크톤 유생시기를 거쳐 기질에 부착하여 생활한다. 따개비류에 관한 국내연구는 순수 분류학적으로 몇 편의 연구가 이루어졌을 뿐이며 실험실 내 사육조건 및 유생의 민감도 등에 관한 연구는 제대로 수행되어 있지 않은 실정이다. 본 연구에서는 첫 번째 단계 유생을 대상으로 몇몇 중금속(Cd, Cr, Cu 그리고 Zn)에 대한 민감도를 조사하였고 타 연구결과와의 비교로 생태 독성학적 적용을 시도하였다. 네 가지 금속물질, 카드뮴, 크롬, 구리 그리고 아연에 대한 따개비 유생의 반수사망농도는 각각 0.43, 24.4, 0.22 그리고 1.74mg/L로 산정되었다. 외국의 생물검정용 따개비와 본 연구에 사용된 고랑따개비의 민감도는 아주 유사하게 나타났다. 따라서 국내산 고랑따개비의 유생은 해양 환경 평가에 일반적으로 이용되는 해양생물과 동반 검정의 수행에 자격을 갖추었다고 할 수 있다. 본 연구의 결과는 해양환경의 평가는 물론 방오물질개발 등에의 기초자료를 제공할 수 있을 것으로 사료된다.

Studies on the Propagation of the Freshwater Prawn, Macrobrachium nipponense (De Haan) Reared in the Laboratory 2. Life History and Seedling Production (담수산 새우, Macrobrachium nipponense (De Haan)의 증${\cdot}$양식에 관한 생물학적 기초연구 2. 생활사 및 종묘생산에 관한 연구)

  • KWON Chin-Soo;LEE Bok-Kyu
    • Journal of Aquaculture
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    • v.5 no.1
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    • pp.29-67
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    • 1992
  • Life cycle and seed production of the freshwater prawn, Macrobrachium nipponense, were studied and the results are as follows : 1. Larval development : Embryos hatched out as zoea larvae of 2.06 mm in mean body length. The larvae passed through 9 zoea stages in $15{\~}20$ days and then metamorphosed into postlarvae measuring 5.68 mm in mean body length. Each zoea stage can be identified based on the shapes of the first and second antennae, exo- and endopodites of the first and second pereiopods, telson and maxillae. 2. Environmental requirements of zoea larvae : Zoea larvae grew healthy when fed with Artemia nauplii. Metamorphosing rate was $65{\~}72{\%}$ at $26{\~}28\%$ and $7.85{\~}8.28\%_{\circ}Cl.$. The relationship between the zoeal period (Y in days) and water temperature (X in $^{\circ}C$) is expressed as Y=46.0900-0.9673X. Zoeas showed best survival in a water temperature range of $26{\~}32^{\circ}C$ (optimum temperature $28^{\circ}C$), at which the metamorphosing rate into postlarvae was $54{\~}72\%$ The zoeas survived more successfully in chlorinity range of $4.12{\~}14.08{\%_{\circ}}Cl.$, (optimum chlorinity $7.6{\~}11.6\;{\%_{\circ}}Cl.$.), at which the metamorphosing rate was $42{\~}76{\%}$. The whole zoeal stages tended to be longer in proportion as the chlorinity deviated from the optimum range and particularly toward high chlorinity. Zoeas at all stages could not tolerate in the freshwater. 3. Environmental requirements of postlarvae and juveniles : Postlarvae showed normal growth at water temperatures between $24{\~}32^{\circ}C$ (optimun temperature $26{\~}28^{\circ}$. The survival rate up to the juvenile stage was $41{\~}63{\%}$. Water temperatures below $24^{\circ}C$ and above $32^{\circ}$ resulted in lower growth, and postlarvae scarcely grew at below $17^{\circ}C$. Cannibalism tended to occur more frequently under optimum range of temperatures. The range of chlorinity for normal growth of postlarvae and juveniles was from 0.00 (freshwater) to $11.24{\%_{\circ}}Cl.$, at which the survival rate was $32{\~}35\%$. The postlarvae grew more successfully in low chlorinities, and the best growth was found at $0.00\~2.21{\%_{\circ}}Cl.$. The postlarvae and juveniles showed better growth in freshwater but did not survive in normal sea water. 4. Feeding effect of diet on zoea Ilarvae : Zoea larvae were successfully survived and metamorposed into postlarvae when fed commercial artificial plankton, rotifers, and Artemia nauplii in the aquaria. However, the zoea larvae that were fed Artemia nauplii and reared in Chlorella mixed green water showed better results. The rate of metamorphosis was $68\~{\%}75$. The larvae fed cow live powder, egg powder, and Chlorella alone did not survive. 5. Diets of postlarvae, juveniles and adults : Artemia nauplii and/or copepods were good food for postlarvae. Juveniles and adults were successfully fed fish or shellfish flesh, annelids, corn grain, pelleted feed along with viscera of domestic animals or fruits. 6. Growth of postlarvae, juveniles and adults : Under favorable conditions, postlarvae molted every five or six days and attained to the juvenile stage within two months and they reached 1.78 cm in body length and 0.17 g in body weight. The juveniles grew to 3.52 cm in body length and 1.07 g in body weight in about four months. Their sexes became determinable based on the appearance of male's rudimental processes (a secondary sex character) on the endopodites of second pereiopods of males. The males commonly reached sexual maturity in seven months after attaining the postlarvae stage and they grew to 5.65 cm in body length and 3.41 g in body weight. Whereas the females attained sexual maturity within six to seven months, when they measured 4.93 cm in body length and 2.43 g in body weight. Nine or ten months after hatching, the males grew $6.62{\~}7.14$ cm in body length and $6.68{\~}8.36$ g in body weight, while females became $5.58{\~}6.08$ cm and $4.04{\~}5.54$ g. 7. Stocking density : The maximum stocking density in aquaria for successful survival and growth was $60{\~}100$ individuals/$\ell$ for zoeas in 30-days rearing (survival rate to postlarvae, $73{\~}80{\%}$) ; $100{\~}300$ individuals/$m^2$ for postlarvae of 0.57 cm in body length (survival rate for 120 days, $78{\~}85{\%}$) ; $40{\~}60$ individuals/$m^2$ for juveniles of 2.72 cm in body length (survival rate for 120 days, $63{\~}90{\%}$) : $20{\~}40$ individuals/$m^2$ for young prawns of 5.2 cm in body length (survival rate for 120 days, $62\~90{\%}$) ; and $10\~30$ individuals/$m^2$ for adults of 6.1 cm in body length (survival rate for 60 days, $73\~100{\%}$). The stocking density of juveniles, youngs and adults could be increased up to twice by providing shelters.

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