Effect of Heavy Metals on Larvae of Barnacle, Balanus albicostatus

생태독성 검정을 위 한 고랑따개비 (Balanus albicostatus) 초기유생의 중금속에 대한 민감도 분석

  • Sung, Chan-Gyoung (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Choi, Mi-Sun (Institute of Environmental Protection and Safety, NeoEnBiz Co.) ;
  • Chun, Ho-Hwan (Advanced Ship Engineering Research Center, Pusan National University) ;
  • Lee, In-Won (Advanced Ship Engineering Research Center, Pusan National University) ;
  • Park, Hyun (Advanced Ship Engineering Research Center, Pusan National University) ;
  • Lee, Chang-Hoon (Institute of Environmental Protection and Safety, NeoEnBiz Co.)
  • 성찬경 ((주)네오엔비즈 환경 안전연구소) ;
  • 최미선 ((주)네오엔비즈 환경 안전연구소) ;
  • 전호환 (부산대학교 첨단조선공학연구센터) ;
  • 이인원 (부산대학교 첨단조선공학연구센터) ;
  • 박현 (부산대학교 첨단조선공학연구센터) ;
  • 이창훈 ((주)네오엔비즈 환경 안전연구소)
  • Published : 2008.12.30

Abstract

따개비류는 암반기질에 무리를 이루어 비교적 단단히 부착생활을 하며 전 세계적으로 분포하는 생물이다. 본 생물은 형태가 다른 두 단계의 플랑크톤 유생시기를 거쳐 기질에 부착하여 생활한다. 따개비류에 관한 국내연구는 순수 분류학적으로 몇 편의 연구가 이루어졌을 뿐이며 실험실 내 사육조건 및 유생의 민감도 등에 관한 연구는 제대로 수행되어 있지 않은 실정이다. 본 연구에서는 첫 번째 단계 유생을 대상으로 몇몇 중금속(Cd, Cr, Cu 그리고 Zn)에 대한 민감도를 조사하였고 타 연구결과와의 비교로 생태 독성학적 적용을 시도하였다. 네 가지 금속물질, 카드뮴, 크롬, 구리 그리고 아연에 대한 따개비 유생의 반수사망농도는 각각 0.43, 24.4, 0.22 그리고 1.74mg/L로 산정되었다. 외국의 생물검정용 따개비와 본 연구에 사용된 고랑따개비의 민감도는 아주 유사하게 나타났다. 따라서 국내산 고랑따개비의 유생은 해양 환경 평가에 일반적으로 이용되는 해양생물과 동반 검정의 수행에 자격을 갖추었다고 할 수 있다. 본 연구의 결과는 해양환경의 평가는 물론 방오물질개발 등에의 기초자료를 제공할 수 있을 것으로 사료된다.

Keywords

References

  1. Barker MF. Culture and morphology of some New Zealand barnacles (Crustacea, Cirripedia), NZ J Mar Freshwater Res 1976; 10: 139-158 https://doi.org/10.1080/00288330.1976.9515605
  2. Beiras R and Albentosa M. Inhibition of embryo development of the commercial bivalves Ruditapes decussatus and Mytilus galloprovincialis by trace metals; Implication for the implementation of seawater quality criteria, Aquaculture 2004; 230: 205-213
  3. Billinghurst Z, Clare AS and Depledge MH. Effects of 4-nnonylphenol and 17b-oestradiol on early development of the barnacle Elminius modestus, J Exp Mar Biol Ecol 2001; 257: 255-268 https://doi.org/10.1016/S0022-0981(00)00338-5
  4. Bookhout GC and Costlow Jr JD. Feeding, molting, and growth in barnacles.-In Marine Boring and Fouling Organism, DL Lay ed. Univ. Washington Press, Seattle, Washington. 1959; pp. 212-225
  5. Branscomb ES and Vedder K. A description of the naupliar stages of the barnacles Balanus glandula Darwin, Balanus cariosus Pallas and Balanus crenatus Bruguiere (Cirripedia, Thoracica), Crustaceana 1982; 42: 83-95 https://doi.org/10.1163/156854082X00722
  6. Brown SK and Roughgarden J. Growth, morphology, and laboratory culture of larvae of Balanus glandula (Cirripedia, Thoracica), J Crust Biol 1985; 5: 574-590 https://doi.org/10.2307/1548236
  7. Calaberse A, Macinnes JR, Nelson DA and Miller JE. Survival and growth of bivalve larvae under heavy metal stress, Mar Biol 1977; 41: 179-184 https://doi.org/10.1007/BF00394024
  8. Costlow JD and Bookhout CG. Larval development of Balanus eburneus in the laboratory, Biol Bull Woods Hole 1957; 112: 313-324 https://doi.org/10.2307/1539123
  9. Dinnel PA, Stober QJ, Link JM, Letourneau MW, Roberts WE, Felton SP and Nakatani RE. Methodology and validation of a sperm cell toxicity test for testing toxic substances in marine waters, Final Report Grant R/Tox-1, University of Washington Sea Grant Program 1983
  10. Dinnel PA, Link JM, Stober QJ, Letourneau MW and Roberts WE. Comparative sensitivity of sea urchin sperm bioassay to metals and pesticides, Archives of Environ Contam Toxicol 1989; 18: 748-755 https://doi.org/10.1007/BF01225012
  11. Dinnel PA. Toxicity testing with oyster and mussel embryos, In G.A. Chapman, (Ed.) Culture and Toxicity Testing of West Coast of Marine Organisms, US EPA Newport, Oregon 1991
  12. Dorn PB, Rodgers JH, Jop Jr KM, Raia JC and Dickson KL. Hexavalent chromium as a reference toxicant in effluent toxicity tests, environ toxicol chem 1987; 6(6):435-444 https://doi.org/10.1897/1552-8618(1987)6[435:HCAART]2.0.CO;2
  13. Eisler R. Chromium hazards to fish, wildlife, and invertebrates: a synoptic review. US Fish Wildl Serv Biol Rep 1986; 85(1.6), 60pp
  14. Finney DJ. Statistical Method in Biological Assay, Griffin, London 1971
  15. Gore JA and Bryant RM. Changes in fish and benthic macroinvertebrate assemblages along the impounded Arkansas River. Jour Freshwater Ecol 1986; 3: 333-338 https://doi.org/10.1080/02705060.1986.9665125
  16. Harrison WG, Eppley RW and Renger EH. Phytoplankton nitrogen metabolism, nitrogen budgets, and observations on copper toxicity: controlled ecosystem pollution experiment, Bull Mar Sci 1977; 27: 44-57
  17. Hiraoka Y, Ishizawa S, Kamada T and Okuda H. Acute toxicity of 14 different kinds of metals affecting medaka Fry, hiroshima, J Med Sci 1985; 34(3): 327-330
  18. Hunt JW and Anderson BS. Sublethal effect of zinc and municipal effluents on larvae of the red abalone Haliotis rufescens, Marine Biology 1989; 101: 545-552 https://doi.org/10.1007/BF00541657
  19. Jones RE. Hexavalent chrome: threshold concept for carcinogenicity, Biomed-Environ-Sci 1990; 3: 20-34
  20. Lang WH, Lawrence S and Miller DC. The effects of temperature, light, and exposure to sublethal levels of copper on the swimming behavior of barneale nauplii, Advances in marine environmental research, proceedings of a symposium, pp. 273-289. Ed. by F. S. Jacoff. Narragansett, Rhode Island: Office of Research and Development, U.S. Environmental Protection Agency(EPA-600/9-79-035) 1979
  21. Lang WH, Marcy M, Clem PJ, Miller DC and Rodelli MR. The comparative photobehavior of laboratory-hatched and plankton-caugh Balanus improvisus (Darwin) nauplii and the effects of 24-hour starvation, J Exp Mar Biol Ecol 1980; 42: 201-212 https://doi.org/10.1016/0022-0981(80)90176-8
  22. Lee C, Shim JM, Jee YJ, Ryu HY, Kim BS and Kim CH. Larval development of a sessile barnacle Balanus improvisus Darwin (Cirripedia: Thoracica), J Fish Sci Tech 1998; 1(1): 72-78
  23. Lee C and Kim CH. Larval development of Balanus trigonus Darwin (Cirripedia: Thoracica: Balanidae) reared in the laboratory, Bull Korean Fish Soc 1990; 23(6): 457-467
  24. Lee C and Kim CH. The larval development of Balanus albicostatus Pilsbry (Cirripedia, Thoracica) reared in the laboratory, J Exp Mar Biol Ecol 1991; 147: 231-244 https://doi.org/10.1016/0022-0981(91)90184-X
  25. Lee C, Shim JM and Kim CH. Larval development of Balanus reticulatus Utinomi, 1967 (Cirripedia, Thoracica) and a comparison with other barnacle larvae, J Plankton Res 1999; 21(11): 2125-2142 https://doi.org/10.1093/plankt/21.11.2125
  26. Martin M, Osborn KE, Bilig P and Glicksten N. Toxicities of ten metals to Crassostrea gigas and Mytilus edulis embryos and Cancer magister larvae, Mar Pollut Bull 1981; 12(9): 305-308 https://doi.org/10.1016/0025-326X(81)90081-3
  27. Meyers E. Chemistry of Hazardous Materials. Prentice Hall Career and Technology, Prentice-Hall Inc., Englewood Cliffs, NJ. 1990; 509pp
  28. Ministry of Education. Korean animal and plant picture book. Daehan Printing & Publishing Co., Ltd., Seoul, Korea. 38th volume, animal 1998; pp. 1038
  29. Moore SB, Winckel J, Detwiler SJ, Klasing SA, Gaul PA, Kanim NR, Kesser BE, Debeveck AB, Beardsley K and Puckett LK. Fish and Wildlife Resources and Agricultural Drainage in the San Joaquin Valley, California. San Joaquin Drainage Program, Sacramento, California. Six sections and 2 appendixes. Portions reproduced with the permission of senior author Stephen Moore, Fish and Wildlife Service, Regional Office, Portland, OR. 1990
  30. Moraitou-Apostolopoulou M and Verriopulos G. Some effects of subqethal concentrations of copper on a marine copepod, Mar Pollut Bull 1979; 10: 88-92 https://doi.org/10.1016/0025-326X(79)90220-0
  31. Nacci D, Jackim E and Walsh R. Comparative evaluation of three rapid marine toxicity test: sea urchin early embryo growth test, sea urchin sperm cell toxicity test and microtox, Environ Toxicol Chemi 1986; 5: 521-525 https://doi.org/10.1897/1552-8618(1986)5[521:CEOTRM]2.0.CO;2
  32. National Library of Medicine. Hazardous Substances Data Bank (HSDB). U.S. National Library of Medicine, 8600 Rockville Pike, Bethesda, MD 20894. 1988
  33. Pyefmch KA and Mott JC. The sensitivity of barnacles and their larvae to copper and mercury, J Exp Biol 1948; 25:276-298
  34. Rand GM and Petrocelli RS (eds.). Fundamentals of Aquatic Toxicology. Hemisphere Publishing Company, NY. 1985; 666pp
  35. Reeve MR, Grice GD, Gibson VR, Walter MA, Darcy K and Ikeda T. A controlled environmental pollution experiment (CEPEX) and its usefulness in the study of larger marine zooplankton under toxic stress. In: Effects of pollutants on aquatic organisms, pp. 145-162. Ed. by Lockwood AP. Cambridge: Cambridge University Press. 1976
  36. Sasikumar N, Clare AS, Gerhart DJ, Stover D and Rittschof D. Comparative Toxicities of Selected Compounds to Nauplii of Balanus amphitrite Darwin and Ademia sp, Bull Environ Contam Toxicol 1995; 54: 289-296
  37. Skattebol L, Nilsen NO, Stenstrom Y, Andreassen P and Willemsen P. The antifouling activity of some juvenoids on three species of acorn barnacle, Balanus, Pest Manag Sci 2006; 62: 610-616 https://doi.org/10.1002/ps.1210
  38. Sokal RR and Rholf FJ. Biometry. 2nd ed Freeman WH and Company, San Francisco, CA. 1981
  39. Sorensen EM. Metal Poisoning in Fish, 374pp. Lewis Publishers, an Imprint of CRC Press, Boca Raton, FL. Reprinted by permission CRC Press. 1991
  40. Thomas KV, Fileman TW, Readman JW and Waldock M. Antifouling paint booster biocides in the UK coastal environment and potential risks of biological effects, Mar Pollut Bull 2001; 42: 677-688 https://doi.org/10.1016/S0025-326X(00)00216-2
  41. Viarengo A. Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at cellular level. Reviews, Aq Sci 1989; 1(2): 295-317
  42. Volpi GA, Arizzi NA, Losso C and Ghetti PF. Sea urchin toxicity bioassays for sediment quality assessment in the Lagoon of Venice (Italy), J Chemi Ecol 2003; 19(2/3):99-111 https://doi.org/10.1080/0275754031000119870
  43. Wells PG. Marine Ecotoxicological tests with zooplankton. Ecotoxicological testing for marine environment, Persoone G Jaspers E and Claus C (eds) State Univ Ghent and Inst Mar Scient Res Breden, Belgium. 1984; 1: 215-257
  44. Yonehara Y. Recent topics on marine antifouling coatings. Bull Soc Sea Water Sci Jpn 2000; 54: 7-12