• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.117-123
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• 2003

For screening of autoregulator receptor gene from Saccharopolyspora erythraea, PCR was performed with primers of receptor gene designed on the basis of amino acid sequences of autoregulator receptor proteins with known function. PCR products were subcloned into the BamHI site of pUC19 and transformed into the E. coli DH5$\alpha$. The isolated plasmid from transformant contained the fragment of 120 bp, which was detected on 2% gel after BamHI treatment. The insert, 120 bp PCR product, was confirmed as the expected internal segment of gene encoding autoregulator receptor protein by sequencing. Southern and colony hybridization using Saccha. erythraea chromosomal DNA were performed with the insert as probe. The plasmid (pEsg) having 3.2 kbp SacI DNA fragment from Saccha. erythraea is obtained. The 3.2 kbp SacI DNA fragment was sequenced by the dye terminator sequencing. The nucleotide sequence data was analyzed with GENETYX-WIN (ver 3.2) computer program and DNA database. frame analyses of the nucleotide sequence revealed a gene encoding autoregulator receptor protein which is a region including KpnI and SalI sites on 3.2 kbp SacI DNA fragment. The autoregulator receptor protein consisting of 205 amino acid was named EsgR by author. In comparison with known autoregulator receptor proteins, homology of EsgR showed above 30%.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.177-184
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• 2007

Bioactive substances, antioxidant activities and tyrosinase inhibitory activities of seed extracts were evaluated to discover new functional materials, using 13 edible or medicinal plants. Total polyphenol and flavonoid contents were highest in Taxus cuspidata, with $57.51mg{\cdot}g^{-1}\;and\;7.98mg{\cdot}g^{-1}$, respectively. Seed extract of Vitis coignetiae${\times}$Vitis labruscana($RC_{30}=0.030mg{\cdot}ml^{-1}$) was found to be the most effective in DPPH radical scavenging test, and the highest ABTS radical scavenging activity was shown in Diospyros lotus($RC_{30}=0.044mg{\cdot}ml^{-1}$). Inhibitory effects on peroxidation of linoleic acid determined by ferric thiocyanate (FTC) method were similar to BHT in Diospyros kaki, Diospyros lotus, Magnolia officinalis, Styrax obassia, Vitis coignetiae, Vitis coignetiae${\times}$Vistis labruscana, Zizyphus jujuba, Zizyphus jujuba var. inermis. For the inhibition against mushroom tyrosinase, Diospyros kaki, Diospyros lotus, Poncirus tritoliata, Prunus serrulata var. spomtanea, Zizyphus jujuba and Zizyphus jujuba var. inermis exhibited inhibitory activity, and especially Diospyros lotus showed the strongest inhibition.

• Journal of The Korean Association For Science Education
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• v.35 no.3
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• pp.509-521
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• 2015

Argumentation is a social and collaborative dialogic process. A large number of researchers have focused on analyzing the structure of students' argumentation occurring in the scientific inquiry context, using the Toulmin's model of argument. Since SSI dialogic argumentation often presents distinctive features (e.g. interdisciplinary, controversial, value-laden, etc.), Toulmin's model would not fit into the context. Therefore, we attempted to develop an analytical framework for SSI dialogic argumentation by addressing the concepts of 'discourse clusters' and 'discourse schemes.' Discourse clusters indicated a series of utterances created for a similar dialogical purpose in the SSI contexts. Discourse schemes denoted meaningful discourse units that well represented the features of SSI reasoning. In this study, we presented six types of discourse clusters and 19 discourse schemes. We applied the framework to the data of students' group discourse on SSIs (e.g. euthanasia, nuclear energy, etc.) in order to verify its validity and applicability. The results indicate that the framework well explained the overall flow, dynamics, and features of students' discourse on SSI.

• Journal of the Korean Geographical Society
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• v.44 no.6
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• pp.797-812
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• 2009

The purpose of this study is to analyze cause-and-effect relationships between patterns of the road network and socio-economic factors such as population, industry, and land use of Seoul during the period of 1970~2000. In this study, Structural Equation Model (SEM) is used to estimate simultaneously the cause-and-effect relationships between many independent variables and dependent variables. For the observed variables, variables related to population, industry, land price, land use, and road variables were included; these variables were classified by exploratory factor analysis. The analysis using the SEM showed that the structure of the model changed around the 1980's. In general, socio-economic factors such as population, industry, and land use affected construction of the road network of the city during the 1970's and the 1980's. Especially, it was analyzed that the industrial development had the greatest impact on formation of the road network. In the 1990's, the effect that the road network exerts on socio-economic factors proved to be more appropriate than the other way round. At that time, the road-related factors had the greatest effect on the land price and industrial development, and that tendency has continued until now.

• Journal of Digital Convergence
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• v.15 no.4
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• pp.339-349
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• 2017

The purpose of this study was to identify the effect of Career Education Program(CEP) on freshmen of nursing department. A non-equivalent pre-post test of quasi-experimental design was used. 44 freshmen were assigned to an intervention group and 36 freshmen to a control group. The intervention program was composed of introduction, self-navigation, explore the world of work, career design and termination during 12 weeks from March to June, 2014. Data were collected before-and after program, and analyzed using the SPSS 22.0 program. There were significant increases in learning motivation (t=8.92, p<.001) and college life adaptation (t=3.51, p<.001) in the experimental group compared to the control group. This study suggests that CEP would be an efficient way to adapt to school and take learning motivation for nursing students. It is necessary to develop a systematic and tailored career education program for nursing students from a freshmen to a senior.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.1
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• pp.29-36
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• 2011

The present study was performed to investigate antioxidant activities of Korean edible wild extracts. In water and methanol extracts of edible wild plants, total polyphenol content of water and methanol extracts ranged 4.6~183.8 and 8.2~270.1 mg/g, respectively. Radical scavenging activity against DPPH and ABTS radicals was observed to be high in Lysimachia barystachys, Aceriphyllum rossii, Securinega suffruticosa, Sedum kamtschaticum, and Pedicularis resupinata. Pedicularis resupinata, Securinega suffruticosa, Patrinia villosa, and Solidago virga-aurea var. asiatica showed effective SOD-like activity. There was significant correlation between polyphenol content and DPPH and ABTS radical scavenging activities, and SOD-like activity. Plant extracts such as Pedicularis resupinata, Securinega suffruticosa, Rhapontica uniflora, Lysimachia barystachys, and Aceriphyllum rossii had higher polyphenol content, radical scavenging, and SOD-like activity. These results indicate that Korean edible wild plants may be useful as potential antioxidant sources for improving human antioxidant defense system.

• Journal of Korean Home Economics Education Association
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• v.36 no.1
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• pp.1-18
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• 2024

The purpose of this study is to explore the direction of home economics education and provide specific implications to help adolescents develop relationship competency. For this purpose, the contents of Korea's character education and UK's relationships education curriculum were analyzed, and the results are as follows. Character education in Korea focused on character education values such as responsibility, respect, consideration, and communication throughout the public education system. These contents were largely consistent with the learning content elements for cultivating relationship-competency in home economics education. UK's relationships education at the primary and secondary levels were similar to that of home economics education in middle school in Korea in its goals and learning contents for understanding and maintaining healthy relationships. However, differences existed in areas of safe relationships, setting boundaries with others, comprehensiveness of sex education content, rights to refuse sex education, and education content based on equal rights. Based on these results, this study concludes that the concept of education on relationships needs to be defined, the composition of educational content should be systematically developed, and further research on the development of teaching and learning methods and learning programs that can elicit and internalize students' attitudes and changes is needed. In addition, insights were gained regarding additional educational contents and considerations to take into account.

• Journal of Gifted/Talented Education
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• v.25 no.2
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• pp.321-337
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• 2015

The purpose of the present study is to examine elementary gifted students' perceptions on bias against creativity utilizing concept mapping approach. Twelve elementary gifted students participated in the group brainstorming and produced 55 final statements. Based on these statements, the multi-dimensional scale and hierarchial cluster analysis using dissimilarity matrix were performed. Average stress value was .30 which is appropriate for a two-dimensional concept mapping study. In addition, a questionnaire survey using likert 6 points scale was carried out targeting 132 elementary gifted students to analyze the degree of sympathy on their anti-creativity bias perception. The findings are as follow: First, four categories were concluded dividing gifted students' perceptions on bias against creativity from the hierarchial cluster analysis with X-Y coordinate matrix, these were 'Contradictory attitudes to creativity', 'Low evaluation for creativity', 'Forced to predetermined rules and ideas', and 'Aversion to new things'. Second, elementary gifted students were sympathetic to the order 'Forced to predetermined rules and ideas'(M=4.16), 'Aversion to new things'(M=3.68), 'Contradictory attitudes to creativity'(M=3.55) and 'Low evaluation for creativity'(M=3.30). This study aims to examine, analyze and categorize various relevant factors related to elementary gifted students' perceptions on bias against creativity. Implications of the study related to the present and future creative education were discussed in depth.

• Childhood Kidney Diseases
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• v.15 no.1
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• pp.38-48
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• 2011

Purpose: Focal segmental glomerulosclerosis (FSGS) is the most common glomerulopathy causing pediatric renal failure. Since specific treatment targeting the etiology and pathophysiology of primary FSGS is yet elusive, the authors explored the pathophysiology of FSGS by transcriptome analysis of the disease using an animal model. Methods: FGS/kist strain, a mouse model of primary FSGS, and RFM/kist strain, as control and the parent strain of FGS/kist, were used. Kidney tissues were harvested and isolated renal cortex was used to extract mRNA, which was run on AB 1700 mouse microarray chip after reverse transcription to get the transcriptome profile. Results: Sixty two genes were differentially expressed in FGS/kist kidney tissue compared to the control. Those genes were related to cell cycle/cell death, immune reaction, and lipid metabolism/vasculopathy, and the key molecules of their networks were TNF, IL-6/4, IFN${\gamma}$, TP53, and PPAR${\gamma}$. Conclusion: This study confirmed that renal cell death, immune system activation with subsequent fibrosis, and lipid metabolism-related early vasculopathy were involved in the pathophysiology of FSGS. In addition, the relevance of methodology used in this study, namely transcriptome profiling, and Korean animal model of FGS/kist was validated. Further study would reveal novel pathophysiology of FSGS for new therapeutic targets.

• Journal of Life Science
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• v.22 no.1
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• pp.74-79
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• 2012

This study was conducted to investigate the production of water-soluble carbohydrates (WSCs) by treatment of different amounts of rice straw with cellulase, hemicellulase, and xylanase. Treatment of high amounts of rice straw (100 g/l) with cellulase and hemicellulase resulted in similar production of WSCs. Reducing the amount of rice straw to 50 g/l decreased the production of WSCs by hemicellulase but had no effect on WSC production by cellulase. The interaction among rice straw amounts, and hemicellulase and xylanase activities was investigated using a Box Behnken design and a response surface model. An interaction was found only between hemicellulase and xylanase. An enzyme mixture consisting of 0.55 mg/ml of hemicellulase and 0.65 mg/ml of xylanase generated the highest amounts of WSCs, regardless of the amount of rice straw provided. Therefore, the activity of cellulase was higher than that of either hemicellulose or xylanase for WSC production from rice straw. The interaction observed for hemicellulase and xylanase indicates that a combined enzyme treatment could improve the production of WSCs from rice straw.