• Title/Summary/Keyword: 유사성 탐색

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Development of Local Animal BLAST Search System Using Bioinformatics Tools (생물정보시스템을 이용한 Local Animal BLAST Search System 구축)

  • Kim, Byeong-Woo;Lee, Geun-Woo;Kim, Hyo-Seon;No, Seung-Hui;Lee, Yun-Ho;Kim, Si-Dong;Jeon, Jin-Tae;Lee, Ji-Ung;Jo, Yong-Min;Jeong, Il-Jeong;Lee, Jeong-Gyu
    • Bioinformatics and Biosystems
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    • v.1 no.2
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    • pp.99-102
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    • 2006
  • The Basic Local Alignment Search Tool (BLAST) is one of the most established software in bioinformatics research and it compares a query sequence against the libraries of known sequences in order to investigate sequence similarity. Expressed Sequence Tags (ESTs) are single-pass sequence reads from mRNA (or cDNA) and represent the expression for a given cDNA library and the snapshot of genes expressed in a given tissue and/or at a given developmental stage. Therefore, ESTs can be very valuable information for functional genomics and bioinformatics researches. Although major bio database (DB) websites including NCBI are providing BLAST services and EST data, local DB and search system is demanding for better performance and security issue. Here we present animal EST DBs and local BLAST search system. The animal ESTs DB in NCBI Genbank were divided by animal species using the Perl script we developed. and we also built the new extended DB search systems fur the new data (Local Animal BLAST Search System: http://bioinfo.kohost.net), which was constructed on the high-capacity PC Cluster system fur the best performance. The new local DB contains 650,046 sequences for Bos taurus(cattle), 368,120 sequences for Sus scrofa (pig), 693,005 sequences for Gallus gallus (fowl), respectively.

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An Exploratory Study of the Relationship between Smart Learning and Smart Work: The Use of Personal Laptops by Graduate Students in a Smart Campus Environment (스마트러닝과 스마트워크의 관련성에 대한 탐색적 연구: 스마트 캠퍼스 환경에서 대학원생의 개인 노트북 컴퓨터 사용을 중심으로)

  • Kim, Young-Woo
    • Journal of Digital Convergence
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    • v.10 no.5
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    • pp.27-35
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    • 2012
  • The importance of smart learning (SL) has been emphasized in schools. Additionally, the significance of smart work (SW) in improving business performance has gained much attention among industries. From theoretical, technological, and environmental perspectives, SL and SW are somewhat similar. Therefore, a case study was performed to find a way to link SL and SW, and a linking model was proposed for this purpose. Because laptops are considered a pivotal element in the technological aspect of SL, graduate students' use of personal laptops in classes (Bring Your Own Laptop, BYOL) was investigated. The results showed that the students reacted positively to the idea of using personal laptops in class and that they expected to learn IT skills more effectively this way. They listed being able to study even after class and the easy accessibility of relevant data as the strengths of BYOL. However, they cited the heaviness of the laptops and occasional loss of focus during classes as the weaknesses of BYOL. Thus, this study showed the possibility of that students who experience SL can perform better in an SW situation. Therefore, if a policy is enacted that allows students to efficiently use laptops, a greater number of educational achievements will be attained on smart campuses and, subsequently, a greater number of smart workers will be produced.

GIS and Geographically Weighted Regression in the Survey Research of Small Areas (지역 단위 조사연구와 공간정보의 활용 : 지리정보시스템과 지리적 가중 회귀분석을 중심으로)

  • Jo, Dong-Gi
    • Survey Research
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    • v.10 no.3
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    • pp.1-19
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    • 2009
  • This study investigates the utilities of spatial analysis in the context of survey research using Geographical Information System(GIS) and Geographically Weighted Regression (GWR) which take account of spatial heterogeneity. Many social phenomena involve spatial dimension, and with the development of GIS, GPS receiver, and online location-based services, spatial information can be collected and utilized more easily, and thus application of spatial analysis in the survey research is getting easier. The traditional OLS regression models which assume independence of observations and homoscedasticity of errors cannot handle spatial dependence problem. GWR is a spatial analysis technique which utilizes spatial information as well as attribute information, and estimated using geographically weighted function under the assumption that spatially close cases are more related than distant cases. Residential survey data from a Primary Autonomous District are used to estimate a model of public service satisfaction. The findings show that GWR handles the problem of spatial auto-correlation and increases goodness-of-fit of model. Visualization of spatial variance of effects of the independent variables using GIS allows us to investigate effects and relationships of those variables more closely and extensively. Furthermore, GIS and GWR analyses provide us a more effective way of identifying locations where the effect of variable is exceptionally low or high, and thus finding policy implications for social development.

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Understanding the Experience of Visual Change Detection Based on the Experience of a Sensory Conflict Evoked by a Binocular Rivalry (양안경합의 감각적 상충 경험에 기초한 시각적 변화탐지 경험에 대한 이해)

  • Shin, Youngseon;Hyun, Joo-Seok
    • Science of Emotion and Sensibility
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    • v.16 no.3
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    • pp.341-350
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    • 2013
  • The present study aimed to understand the sensory characteristic of change detection by comparing the experience of detecting a salient visual change against the experience of detecting a sensory conflict evoked by a binocular mismatch. In Experiment 1, we used the change detection task where 2, 4, or 6 items were short-term remembered in visual working memory and were compared with following test items. The half of change-present trials were manipulated to elicit a binocular rivalry on the test item with the change by way of monocular inputs across the eyes. The results showed that change detection accuracy without the rivalry manipulation declined evidently as the display setsize increased whereas no such setsize effect was observed with the rivalry manipulation. Experiment 2 tested search efficiency for the search array where the target was designated as an item with the rivalry manipulation, and found the search was very efficient regardless of the rivalry manipulation. The results of Experiment 1 and 2 showed that when the given memory load varies, the experience of detecting a salient visual change become similar to the experience of detecting a sensory conflict by a binocular rivalry.

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Screening of Medicinal Plants for Development of Functional Food Ingredients with Anti-Obesity (항비만 기능성 식품소재 개발을 위한 생약 추출물의 탐색)

  • Kim, Yeong-Jin;Kim, Bo-Hye;Lee, Sun-Yi;Kim, Min-Soo;Park, Chan-Sun;Rhee, Moon-Soo;Lee, Kang-Hyun;Kim, Dong-Seob
    • Applied Biological Chemistry
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    • v.49 no.3
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    • pp.221-226
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    • 2006
  • In order to search anti-obesity agents, the methanol extracts of 155 herbal medicines were screened using with pancreatic lipase, which is involved in conversion of triglycerol to fatty acid. Among the tested medicinal plants, methanol extracts of Amsonia elliptica, Arecae pericarpium, Biota orientalis, Cinnamomum cassia, Curcuma aromatia, Elsholtzia ciliate, Glycyrrhiza uralensis, Mucunae Caulis, Rhus javanica, and Rubus coreanus showed potent inhibition at final concentration of $200\;{\mu}g/ml$ on pancreatic lipase activity. All of them were extracted into chloroform fraction. The relative inhibitory activities against pancreatic lipase by orlistat, the chloroform fraction of Arecae pericarpium and Cinnamomum cassia were 89, 80 and 80%, respectively. These results demonstrated that the screened medicinal plants could be develop as the effective lipase inhibitors in preventing and ameliorating obesity of human beings.

Investigation on Beef Quality Indicator of Off-Flavor Development during Storage (쇠고기 저장 중 이취발생에 대한 Indicator 탐색)

  • Byeon, Ko-Eun;An, Soo-Rim;Shim, Soo-Dong;Lee, Jung-Young;Hong, Kwang-Won;Min, Sang-Gi;Lee, Seung-Ju
    • Food Science of Animal Resources
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    • v.29 no.3
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    • pp.325-333
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    • 2009
  • Beef quality indicators of off-flavor development during storage were investigated in terms of temperature dependence. The off-flavor development time (ODT) was detected using the R-index sensory test. During varying storage conditions at $25^{\circ}C$, $15^{\circ}C$, and $5^{\circ}C$, elements of beef quality were measured, such as volatile basic nitrogen (VBN), pH, color (CIE $L^*$, $a^*$, $b^*$), Warner-Bratzler shear force (WBSF), Pseudomonas spp. CFU, and lactic acid bacteria (LAB) CFU. A model with temperature dependence of ODT during storage was developed using Arrhenius-like equation, and a requirement with quality indicators was mathematically derived, resulting in similar temperature dependence. The temperature dependence of beef quality indicators was represented by the Arrhenius activation energy (Ea). Upon comparing the Ea of beef quality indicators and ODT, the temperature dependence similarity was found to be higher in the order of three groups: VBN, pH, $a^*$ value; LAB, Pseudomonas spp.; and WBSF, $L^*$ value, $b^*$ value. Therefore, VBN were determined as the most effective indicator of beef quality during off-flavor development.

The Development and Validation of the Leisure Obsession Scale (여가강박 척도의 개발 및 타당화 연구)

  • Jiyeon Yoon;Seung-Hyuk Choi;Taekyun Hur
    • Korean Journal of Culture and Social Issue
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    • v.19 no.2
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    • pp.235-257
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    • 2013
  • The purpose of this study is to develop the Leisure Obsession Scale and examine the validity of the scale. The Leisure Obsession Scale was developed and identified its validity by exploratory factor analysis, confirmatory factor analysis, and correlation analysis. The Leisure Obsession Scale consists of two factors, which are 'Leisure Preoccupation' and 'Leisure Stereotype'. Those two factors indicated the reasonable fit index by confirmatory factor analysis. In addition, this scale displayed discriminant validity via measurement of obsession, workaholism, leisure anxiety, and leisure constraint. Also, the results of criterion validation analysis shows that the Leisure Obsession Scale and its subscale are correlated with measure of age, leisure information searching, intention of participation to new leisure activities, and intention of increase in leisure time. Conceptualizing leisure obsession and exploring components of leisure obsession would be valuable for understanding the nature of leisure obsession and its effects on leisure satisfaction, and suggesting more effective psychological intervention in a diverse population.

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Development of a CAPS Marker Derived from the Pg-Actin Gene Sequences and RAPD Markers in Platycodon grandiflorum (도라지에서의 RAPD 마커 분석과 Actin 유전자 염기서열에서 유래한 CAPS 분자표지 개발)

  • Kim, Munhwi;Jeong, Eunah;Jeong, Jeongsu;Kwon, Soontae;Jeon, Ikjo;Jeong, Jeong Hag;Lee, Je Min;Yeam, Inhwa
    • Korean Journal of Plant Resources
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    • v.28 no.5
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    • pp.648-655
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    • 2015
  • Balloon flower (Platycodon grandiflorum A. DC.) is a perennial plant of mainly Campanulaceae family, which have been widely used as a food ingredient and herbal medicine in East Asia. Although demands on related products and yearly cultivation area for balloon flower are increasing, diverse fundamental technologies and molecular breeding studies are not very well supported in Platycodons. In this study, 30 random amplification of polymorphic DNA (RAPD) primers were test in an attempt to explore genetic diversities. In addition, sequences information of the actin gene, a well conserved gene encoding a globular protein that forms microfilaments, was retrieved and analyzed. Two actin homologs were recovered; 3.4 kb fragment is a Pg-actin and 1.4 kb fragment is a Pg-actin homolog with 28.6% similarity. We have confirmed that the Pg-actin gene is configured into 4 exons and 3 introns. A single nucleotide polymorphism (SNP), G↔A, was detected on the intron 3, which served as a target for the CAPS marker development. The marker Pg-Actin-Int3 was applied to 32 balloon flower accessions. Balloon flower DNA sequence information generated in this study is expected to contribute to the analysis and molecular breeding and genetic diversity analysis of balloon flowers.

Screening of Bioactive Compounds from Mushroom Pholiota sp. (비늘버섯으로부터 생리기능성 물질의 탐색)

  • Yu, Hyung-Eun;Cho, Soo-Muk;Seo, Geon-Sik;Lee, Byeong-Seok;Lee, Dae-Hyoung;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.34 no.1
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    • pp.15-21
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    • 2006
  • Extracts from 63 kinds of Pholiota sp. fruiting bodies were prepared using water and methanol, and then their physiological functionalities were investigated. The methanol extracts from Pholiota adiposa PAD030 showed high fibrinolytic activity and those of P. adiposa ASI PAD-022 showed potential inhibitory activity of 76.8% against ${\beta}-hydroxy-{\beta}-methylglutaryl(HMG)-CoA$ reductase. The highest antioxidant and tyrosinase inhibitory activities were found in the water extracts of Pholiota sp. PSP-015 (72.7%) and methanol extracts of P. nameko PNA-024 (69.5%), respectively. However, superoxide dismutase(SOD)-like activity and elastase inhibitory activity were low in almost of the extracts. The HMG-CoA reductase inhibitor from the fruiting body of P. adiposa PAD-022 which showed the highest functionality was extracted maximally when powder of the fruiting body was shaked at $30^{\circ}C$ for 12 h by methanol and its HMG-CoA reductase inhibitory activity was 80.2%.

A Multiple-Item Scale for Measuring Food Service Quality - An Application of the Hierarchical Service Quality Approach - (외식 서비스 품질(FOODSERVQUAL) 측정을 위한 다문항 척도 - 위계적 서비스 품질 모형에의 적용 -)

  • Kim, Sang-Ho
    • Culinary science and hospitality research
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    • v.15 no.4
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    • pp.227-244
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    • 2009
  • This study was conducted to develop a multiple-item scale for measuring the food service quality in the restaurant industry. For developing the scale, Kelly Repertory Grid Methods were conducted. Collected data of food service quality were analyzed with the reliability analysis and the factor analysis of SPSS 12.0 and the 3rd-order confirmatory factor analysis of LISREL 8.70. The food service quality model of this study is conceptually based on the Brady and Cronin(2001)'s hierarchical approach to the service quality model. The hierarchical model of the food service quality which comprises three constructs of the physical environment quality, the interaction quality and the food quality as a primary dimension. The hierarchical approaches to the food service quality help overcome the limitations of the SERVQUAL model for which some arguments have been made that it lacks a clear division between the dimensions and its subsequent overlapping between them.

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