• Korean Journal of Soil Science and Fertilizer
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• v.17 no.1
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• pp.67-76
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• 1984

This experiment was conducted to find out the changes in microflora of submerged soil uncultivated rice plant by application of CNP herbicide (2, 4, 6 - Trichlorophenyl-4-Nitrophenyl ether) under conditions applied with compost, rice straw, glucose or without organic material. The soil, sandy loam textured was incubated in green house for 66 days. Sampling and analysis of microorganisms were carried out during submergence periods. The results were summarilized as follows. 1. Number of aerobic total bacteria was increased by application of CNP herbicide during submerbed 50 days, afterthen, could not seen the difference. The application of rice straw increased number of aerobic bacteria regardless of CNP herbicide application or not, but glucose tended to decrease. 2. Number of Fungi was constantly maintained at $8-20{\times}10^3$ levels during period of submergence regardless of application of CNP herbicide and organic materials or not. 3. The CNP herbicide application tended to decrease the number of actinomycetes, particularly, in the treatments without organic substances and rice straw were remarkably decreased. 4. Anaerobic-and gram-negative bacteria populations were not showed any difference by application of herbicide and organic materials. 5. The ratios of aerobic bacteria to fungi and aerobic bacteria to actinomycetes appeared high values by application of herbicide and of organic substances. 6. At 66 days after submergence, the ratio of chromogenic actinomycetes to the total number of actinomycetes was lowered in application of herbicide. On the other hand, the percentage of both pretense-positive and cellulase-positive actinomycetes to the total isolates were higher in the treatment with herbicide than An without herbicide, particularly. The ratios of pretense-positive actinomycetes were high in the rice straw application regardless of herbicide application or not, but cellulase-positive actinomycetes was not remarkably difference.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.73-82
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• 2008

The resource plants of the Mt. Geonji was listed 354 taxa; 92 families, 242 genera, 303 species, 48 varieties and 3 forms. 354 taxa listed consists of 205 taxa of edible plants(57.1%),234 taxa of medicinal plants(65.2%), 167 taxa of ornamental plants(46.5%) and 218 taxa of the others(60.7%). Specific plant species by floral region were total 22 taxa; Trapella sinensis var. antennifera in class IV, Iris ensata var. spontanea in Class II, 16 taxa(Salix glandulosa, Alnus hirsuta, Chrysosplenium flagelliferum, Mallotus japonicus, Ilex macropoda, Grewia biloba var. parviflora, Vaccinium oldhami, Lysimachia barystachys, Fraxinus mandshurica, etc.) in class I. The naturalized plants in this site were 12 families, 23 genera, 28 species, 2 varieties, 30 taxa(Bromus unioloides, Phytolacca americana, Oenothera erythrosepala, Ipomoea hederacea var. hederacea, Aster pilosus, Erechtites hieracifolia) and naturalization rate was 8.5% of all 354 taxa vascular plants. Wild plants disturbing ecosystem like Solanum carolinense and Ambrosia artemisiifolia var. elatior have been increasing. So, it needs continuing control and conservation measures on the plant ecosystem.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.1-4
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• 2008

The clonal variation in female flowering was studied in Larix leptolepis clone bank, consisting of 116 clones, for three years. The between-year variation was large; i.e. the percentage of flowering grafts and average number of flowering per graft were $28.4{\sim}67.2$ and $9{\sim}176$, respectively. Differences in flowering abundance among clones were large and statistically significant in all the years studied. The variance of flowering abundance among clones was increased when flowering was poor. The average of broad-sense heritability of flowering abundance was 0.52. The genetic gain(%G) was estimated at 57.4% when the upper 30% clones were selected. The clonal stability of flowering abundance was compared using average number of flowering and coefficient of variance value of each clone. The clones such as Gyeonggi 9(29), Kangwon 37(137), Chungnam 6(46), Chungnam 14(414), R11, R8 showed abundant flowering and high stability.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.833-847
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• 2016

Drought stress is a major agricultural limitation to crop productivity worldwide, especially by which leafy vegetables, plant leaves eaten as vegetable, could be more lethal. The study was carried out to know the effect of drought tolerance plant growth promoting bacteria (PGPB) on water stress of kale seedlings. A total of 146 morphologically distinct bacterial colonies were isolated from bulk soil and rhizosphere soil of leafy vegetables and screened for plant growth promoting microbioassay in greenhouse. Out of them the isolate SB19 significantly promoted the growth of kale seedlings in increasement of about 42% of plant height (14.1 cm), 148% of leaf area ($19.0cm^2$) and 138% of shoot fresh weight (1662.5 mg) attained by the bacterially treated plants compared to distilled water treated control (9.9 cm, $7.7cm^2$, 698.8 mg). Shoot water content of SB19 treated kale seedlings (1393.8 mg) was also increased about 152% compared with control (552.5 mg). The SB19 isolated from bulk soil of kale plant in Iksan, Korea, was identified as species of Bacillus based on 16S rRNA gene sequencing analysis. We evaluated the effect of drought tolerance by the Bacillus sp. SB19 on kale seedlings at 7th and 14th days following the onset of the water stress and watering was only at 7th day in the middle of test. In the survey of 7th and 14th day, there were mitigation effect of drought stress in kale seedlings treated with $10^6$ and $10^7cell\;mL^{-1}$ of SB19 compared to distilled water treated control. Especially, there were more effective mitigation of drought damage in kale seedlings treated with $10^7cell\;mL^{-1}$ than $10^6cell\;mL^{-1}$. Further, although drought injury of bacterially treated kale seedlings were not improved at 14th day compared with 7th day, drought injury of $10^7cell\;mL^{-1}$ of SB19 treated kale seedlings were not happen rapidly but developed over a longer period of time than $10^6cell\;mL^{-1}$ of SB19 or control. The diffidence of results might be caused by the concentration of bacterial suspension. This study suggests that beneficial plant-microbe interaction could be a important role of enhancement of water availability and also provide a good method for improving quality of leafy vegetables under water stress conditions.

• Korean Journal of Organic Agriculture
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• v.10 no.1
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• pp.75-86
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• 2002

This study was conducted to investigate the effects of pig manure composting using starch pulp m growth and yield characteristics of potato cropping. Four treatments ; No compost, 1 : 1 : 0, 1 : 0.75 : 0.25 and 1 : 0.5 : 0.5 the mixing ratio of pig manure, saw dust, and dehydrated starch pulp and composting by Piling and blowing methods. Plant heights in first growth stage were higher for the treatment of saw dust compost and starch pulp compost than the treatment of chemical fertilizer, but after the stage, there were no significant difference among treatments. The number of stolons were met for 10.9 in 1 : 0.5 : 0.5 treatment, following 1 : 0.75 : 0.25 and 1 : 1 : 0. On the other hand, tuber diameter and top dry matter weight tended to be larger for manure treatment than no treatment but there was no significant difference. Total number of tubers were largest for 1 : 0.5 : 0.5, and those for 1 : 1 : 0 and 1 : 0.75 : 0.25 were similar. Tuber yields of not more than 80g tended to be different, but those of between 81g and 120g and more than 120g were apparently larger for the compost treatment than no treatment. The ratio of marketable tubers appeared large to be about 86% for 1 : 0.75 : 0.25 and 1 : 0.5 : 0.5 treatments. Ratio of infected common scab on potato tubers tended to be highest for 1 : 0.5 0.5 but there were no statistical significance. However, when compost was made by mixing starch pulp in future, the solutions to the occurrence of infected common scab must be considered, The contents of N. P, K and Ca in leaves were larger for the compost treatment than no treatment, but no significant difference was observed, Accordingly, the effects of treating starch pulp compost on growth and yield characteristics of potato cropping were more affirmative than those of saw dust compost.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.124-130
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• 2006

Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.

• Journal of the Korea Organic Resources Recycling Association
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• v.9 no.1
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• pp.56-64
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• 2001

Composting of livestock feces is economic and safe process to decrease the possibility of direct leakage of organic pollutants to ecosystem from commercial and environmental point of view. This study was conducted with three different experiments related to composting of livestock feces. The purpose of experiment 1 was to investigate changes of characteristic of compost pile during composting period by low temperature in cold season. To compare composting effect of experimental compost pile and control pile exposed in cold air, experimental compost piles were warmed up by hot air until their temperatures were reached at $35^{\circ}C$. Sawdust, Ricehull and Ricestraw were mixed with livestock feces as bulking agent. The highest temperatures of compost pile during composting period were in sawdust, rice hull, rice straw, and control were $75^{\circ}C$, $76^{\circ}C$, $68^{\circ}C$, $45^{\circ}C$ respectively. Moisture content, pH, C/N and volume of compost were decreased during composting period. Experiment 2 was carried out to study utilization effect of compost by plant. A corn was cultivated for 3 years on fertilized land with compost and chemical fertilizer. The amount of harvest and nutrition value of corn were analyzed. In first year of trial, the amount of harvest of corn on land treated with compost was lower by 20% than that of land treated with chemical fertilizer. In second year, there was no difference in yield of com between compost and chemical fertilizer. In third year, the yield of com on land fertilized with compost was much more than that of land fertilized with chemical fertilizer. The purpose of experiment 3 was to estimate the decrease of malodorous gas originating from livestock feces by bio-filter. Four types of bio-filters filled with saw dust, night soil, fermented compost and leaf mold were manufactured and tested. Each bio-filter achieved 87-95% $NH_3$ removal efficiency. This performance was maintained for 10 days. The highest $NH_3$ removal efficiency was achieved by leaf mold on the first day of operation period. It reduced the concentration of $NH_3$ by about 95%. Night soil and fermented compost showed nearly equal performance of 93 to 94% for 10 days from the beginning of operation. The concentration of hydrogen sulfide and methyl mercaptan originating for compost were equal to or less than $3mg/{\ell}$ and $2mg/{\ell}$, respectively. After passing throughout the bio-filter, hydrogen sulfide and methyl mercaptan were not detected.

• Journal of the Korea Organic Resources Recycling Association
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• v.13 no.4
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• pp.89-99
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• 2005

The golf courses more than about 200 are operating in Korea. From the golf courses, a great amount of turfgrass clippings tend to increase, steadily. Materials used in the experiment were Creeping Bentgrass(CB), Kentucky Bluegrass(KB), Korean Lawngrass(KL), rice bran and composted chicken drop. Treatments are CB, KB, and KL. The temperature during the composting of all treatments increased rapidly and reached at the highest temperature($57.9^{\circ}C$, $67.8^{\circ}C$, $74.3^{\circ}C$) within 20 days, and then stabilized to the range of $35.2{\sim}41.6^{\circ}C$ at the 30th day. The pH values of all treatments decreased on the first day. However, they were increased rapidly after three days and decreased again on 10~20 days. The pH values of all treatments at the final day were stabilized to the low alkali levels. The contents of total carbon during the period of composting tend to decrease and total nitrogen was increased for factor of reduction of volume. CEC value of all treatments during the period of composting tends to increase. The round paper chromatogram of extracted solution of KL sample was the sharpest and clearest among all treatments. The G.I. values of CB, KB, and KL in 30th day of composting were about 95.1, 77.7, and 98.7 in germination test using chinese cabbage, respectively. Conclusively, all turfgrass clippings used in this experiment were composted well, suitable as composting products standardized by KSC. The maturity of the final compost samples is best in KL, followed by CB and KB treatments. The turfgrass compost can contribute to the plant cultivation for environment-friendly farm, and the results of this study can become the basic data of turfgrass clippings compost. Further research on the mixing ratio of each material is required to produce compost of good quality.

• Korean Journal of Organic Agriculture
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• v.14 no.1
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• pp.69-83
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• 2006

This experiment was conducted to investigate effects of application of diluted and undiluted cattle slurry with water on seasonal and annual dry matter yields and feed values of tall fescue in the uncultivated rice paddy and it was compared with chemical fertilizer. Annual dry matter yields for diluted (average 6.43 ton DM/ha) and undiluted (average 6.56 ton DM/ha) cattle slurry were significantly higher (p<0.05) than those of no fertilizer (3.82 ton DM/ha). This trend was much more conspicuous in treatments applied in spring. In chemical fertilizer treatments, fertilizers with P and K (6.12 ton DM/ha), and P, K and N (10.13 ton DM/ha) had significantly (p<0.05) higher dry matter yields compared with no fertilizer. However, annual dry matter yields for treatments of P and K mixture tended to be lower than those of cattle slurry applications. The efficiency of DM production for inorganic nitrogen in chemical fertilizers annually averaged 26.7kg DM/kg N. In terms of cutting frequencies, it was highest in 2nd growth followed by 1st and 3rd growth. On the other hand, efficiencies of annual DM production of nitrogen for diluted and undiluted cattle slurry were 18.3 and 17.4 kg DM/kg N, respectively, especially, highest in 2nd growth. While, efficiencies of DM production for cattle slurry versus for inorganic nitrogen were 68.5 (undiluted) and 65.2% (diluted), respectively. For annual crude protein contents of tall fescue, no fertilization (11.5%) was significantly higher than chemical fertilizer, but that was lower than cattle slurry ($12.4{\sim}12.6%$) diluted with water. on the contrary, no fertilizers had significantly lower NDF (64.1%) and ADF (37.2%) contents than those of any other treatments, but their RFV (87.0) was significantly higher (p<0.05) than other treatments. The application of cattle slurry and their dilution significantly increased yields of crude protein and total digestible nutrients compared with no and/or P and K fertilizers (p<0.05). This trend was more conspicuous in cattle slurry applied in the early spring.

• Korean Journal of Weed Science
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• v.18 no.3
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• pp.205-213
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• 1998

This experiment was carried out to produce herbicide resistant potatoes hawing only chimeric phosphinothricin acetyltransferase (PAT) genes without using antibiotic selectable marker. The pDY502 vector having only PAT gene was reconstructed for transformation of potato. The reconstructed vector was introduced to Agrobacterium tumefaciens MP90 disarmed, and they were used for potato transformation. Hormonal requirement for plant regeneration from leaves and stem explants of potato was investigated. From this experiment, MS medium treated with IBA 0.1 mg/L + BA 0.5 mg/L was the best for potato regeneration, and the ratio of shoot regeneration was 54% for leaf and 46% for stem in that condition. For transformation, explants of potato leaves and stems were cocultured with A. tumefaciens MP90 containing reconstructed vector harvoring only PAT gene. When the potato explants were placed on various concentrations of bialaphos and all the potato explants were dead on medium with over 5.0mg/L bialaphos. By this selection methods, the explants cocultured with Agrobacterium produced the putative transgenic shoots on medium with 5mg/L bialaphos treatment after 3-4 weeks. Second selection was performed by transferring the shoot tips of putative transgenic to medium containing 20mg/L of bialaphos. The shoot tips grew well on the second selection medium, indicating the production of successful transgenic plants. But normal shoots were dead in same cytotoxic medium. Incorporation of the PAT gene into transgenic potatos were confirmed by PCR analysis of DNA and Southern hybridization. These results show that the PAT gene can serve as a selectable marker and herbicide resistant genes for transformation of potato.