• Laboratory Medicine Online
• /
• v.1 no.1
• /
• pp.57-63
• /
• 2011

Background: ABO antibody titration is useful for the evaluation of ABO-incompatible bone marrow or solid organ transplantations, yet the results quite vary between different test methods used. We compared the results of microcolumn agglutination and tube methods. Methods: Anti-A and anti-B isoagglutionin titers were determined in 63 healthy individuals (23 O, 20 A, and 20 B blood groups) using 4 different methods: immediate spin tube (tube), microcolumn agglutination without anti-human globulin (AHG) (CAT), tube with AHG (tube-AHG) and microcolumn agglutination with AHG (CAT-AHG). Results: The median (range) titers of anti-A and anti-B in group O individuals by tube, CAT, tube-AHG, and CAT-AHG methods were 64 (8-512), 64 (8-512), 128 (8-2,048), and 128 (16-2,048); 64 (16-128), 128 (16-256), 128 (16-512), and 256 (16-512), respectively. The median (range) titers of anti-A in group B and anti-B in group A individuals by the four methods were 64 (16-128), 128 (8-128), 128 (8-256), and 256 (8-256); 64 (8-128), 64 (8-128), 32 (8-128), and 64 (8-256), respectively. The isoagglutinin titer measured by CAT-AHGmethod was the highest. The titers measured by CAT and CAT-AHG methods were 0-1 titer higher than those by tube and tube-AHG methods, respectively. Whatever method was used, the isoagglutinin titers were higher in women than in men. Conclusions: CAT-AHG was the most sensitive method among the four methods tested. Since AHG titer values are critical for the clinical management and CAT has less manual procedures than tube method, CAT-AHG method could be used for the standardization of ABO antibody titration in different institutions.

• The Journal of the Korea Contents Association
• /
• v.14 no.3
• /
• pp.346-351
• /
• 2014

Accurate blood typing tests are essential for safe blood transfusion. Recently many automated test equipments have been introduced to reduce errors and increase the efficiency of the test. However, those equipments being high in price, it is difficult to introduce automated test equipment for every hospital. In this study, we developed a reader for blood typing using column agglutination test. In the process, the results, read out by the image processing, are stored and reaffirmed. To evaluate the reader, 148 samples for ABO and RhD blood typing tests and 154 samples for unexpected antibody test were used. The positive and negative intensity of the reading and the reading of the reaction were 100% in agreement with the result of traditional manual method. If additional verification is completed, this reader can be efficiently and economically used in small-and medium-sized hospitals.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.19 no.8
• /
• pp.1927-1939
• /
• 1995

An experimental investigation is conducted to find out the large scale coherent structures in the intermediate wake past a rotating cylinder with a single tripping wire attached. Relation between the vortex shedding frequency and the spin rate of rotating cylinder and the effects of the tripping wire on the flow characteristics were studied by using spectral analysis and conditional phase average technique, respectively. It is found that the vortex shedding frequency is bound to a certain range and varies regularly as spin rate increases. The coherent structures are compared with those of the plain rotating cylinder in the case of spin rate of 1.0. Distance between the upper and lower center of vortices increase and the vortex shedding time is delayed, the velocity fluctuation energy decreases near the center line of vortices and it spreads out to the outer region. The Reynolds shear stress increases highly in the upper region and the turbulent wake width expands with strong entrainment process.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.18 no.5
• /
• pp.1310-1321
• /
• 1994

Turbulent coherent structures in the intermediate wake of a stationary and rotating cylinder, spin rate S=0.7, situated in a uniform were experimentally investigated using a conditionalphase average technique. Measurements were carried out at a section of 8.5 diameters downstream form the center of cylinder and a Reynolds number of $Re=6.5{\times}10^{3}.$/TEX> The phase averaged velocity and velocity vector fields, contours of vorticity, turbulent intermittency function and velocity fluctuation energy are presented and discussed in relation to the large scale coherent structures by Karman vortices that shed periodically from the cylinder. Coherent wake structures of the rotating cylinder is almost identical with stationary cylinder, but the lateral displacement and shrinkage of turbulent wake region is occured by rotation. Rotation of the cylinder result in that the deflection of wake center to deceleration region(Y/D${\simeq}-0.3)$ and the decrease of mean velocity defect(10%), vorticity strength of large scale structures(19%), total velocity fluctuation energy(12%).

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
• /
• 1997.10a
• /
• pp.52-54
• /
• 1997

고주파 유도결합 플라즈마(RFICP)에서 루우프법에 의해 자기장특성을 계측하였다. 자기장 계측은 플라즈마의 거시적 변화를 시간적으로 접근하며, 반도체 프로세스의 관건인 균일하고, 고집적인 분포를 얼마나 교란, 응집하는가를 검증하고, 밀도와의 관계를 비교, 분석하여 방적의 최적화를 규명할 수 있을 것이다. 작은 루우프 안테나($\Phi$:외경 7.5mm)는 RF 자기장의 크기와 방향을 결정하기 위해 방전속에 삽입된다. 자기장의 세기는 전형적으로 입력파워 50 - 500 [W]에 대해 0.1에서 2.5 G 사이로 변화하였다. 사용가스는 아르곤가스(99.9% 고순도)를 사용하였으며, 동작압력은 20 [mTorr] 에서 15 [sccm]까지하였다. 반경방향의 공간분포에서는 아스펙트비(aspect ratio : R/L)를 2로 하여 자기장 분포를 계측하였다. 자기장은 입력파워의존성에 대해서 200 [W]까지 상승하고, 300[W]에서 안정성을 지속한다. 압력에 대한 의존성은 300[W]에서 60 [mTorr]이상 일 때는 플라즈마의 균질한 압력상태를 벗어남을 보인다. 아르곤 가스유량에 대해서는 무거운 중성기체입자가 자기장의 영향을 거의 받지 않기 때문에 일정한 경향이 나타났다. 반경방향의 공간분포 측정에서는 자기장은 RFICP의 대구경 특성에 맞게 전체적으로 일정한 분포를 이루고 있음을 확인하였다. 이러한 결과로부터 고주파유도결합 플라즈마에서의 동작생성, 유지기구등의 파악에 도움이 될 것이다.

• Korean Journal of Clinical Laboratory Science
• /
• v.49 no.4
• /
• pp.390-394
• /
• 2017

Unexpected antibody screening and identification tests are highly important in the prevention of hemolytic transfusion reactions. Therefore, it is highly recommended to perform unexpected antibody screening test in all transfusion candidates. Here, the frequency and distribution of unexpected antibodies identified in Jeju for the past 3 years were evaluated. Between Jan 2014 and Dec 2016, unexpected antibody screening test was performed for 10,360 sera of transfusion candidates in Jeju general hospital using a column agglutination method with the Ortho BioVue system (Ortho-clinical Diagnostics, Raritan, NJ, USA). Eighty-seven (0.84%) of 10,360 cases that underwent unexpected antibiotics screening showed positive results. Among them, unexpected antibodies were identified in 41 cases (0.40%). Unidentified antibodies were detected in 8 cases (19.51%) and autoantibodies were detected in 3 cases (7.32%). The anti-E antibody included in warm antibodies were detected most frequently in 8 cases (19.51%); 6 cases (14.63%) of anti-E + anti-c antibody and 3 cases (7.32%) of $anti-Le^a+anti-Le^b$. $Anti-Le^a$ and $anti-Le^b$ antibodies were detected in 2 cases (4.88%), respectively. The anti-D, $anti-Di^a$, $anti-Fy^b$, $anti-Jk^a$, $anti-Jk^b$, anti-M and anti-P1 were detected in 1 case (2.44%). Complex antibodies were detected in 1 case (2.44%) in anti-C+anti-D and anti-E+anti-c+$anti-Jk^b$, respectively. In this study, we analyzed the frequency and distribution of unexpected antibodies in one general hospital for the past 3 years. However, there has been a general increase in multicultural families and foreign workers in Jeju, and it would be a meaningful study to compare the frequency and distribution of unexpected antibodies.

• The Korean Journal of Blood Transfusion
• /
• v.23 no.2
• /
• pp.127-135
• /
• 2012

Background: Despite modern advances in laboratory automated medicine, work-process in the blood bank is still handled manually. Several automated immunohematological instruments have been developed and are available in the market. The IH-1000 (Bio-Rad Laboratories, Hercules, CA, USA), a fully automated instrument for immunohematology, was recently introduced. In this study, we evaluated the performance of the IH-1000 for ABO/Rh typing and irregular antibody screening. Methods: In October 2011, a total of 373 blood samples for ABO/Rh typing and 303 cases for unexpected antibody screening were collected. The IH-1000 was compared to the manual tube and slide methods for ABO/Rh typing and to the microcolumn agglutination method (DiaMed-ID system) for antibody screening. Results: For ABO/Rh typing, concordance rate was 100%. For unexpected antibody screening, positive results for both column agglutination and IH-1000 were observed in 10 cases (four cases of anti-E and c, three of anti-E, one of anti-D, one of anti-M, and one of anti-Xg) and negative results for both were observed in 289 cases. The concordance rate between IH-1000 and column agglutination was 98.7%. Sensitivity and specificity were 90.9% and 99.3%, respectively. Conclusion: The automated IH-1000 showed good correlation with the manual tube and slide methods and the microcolumn agglutination method for ABO-RhD typing and irregular antibody screening. The IH-1000 can be used for routine pre-transfusion testing in the blood bank.