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Immobilization of $\beta-galactosidase$ with High Transgalactosylation Activity from Bacillus sp. A4442 Mutant (변이주 Bacillus sp. A4442가 생산하는 갈락토스 전이활성이 높은 $\beta-galactosidase$의 고정화)

  • Kim, Min-Hong;Jung, Jin;In, Man-Jin
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.333-337
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    • 1996
  • For continuous production of galactooligosaccharides(GOS), $\beta-galactosidase$ with h1gh transgalactosylation activity from Bacillus sp. A 4442 was Immobilized onto $Diaion^{TM}$ HPA 75(styrene-divinylbenzene resin). The parameters influencing enzyme immobilization were scrutinized in order to maximize immobilization yield while minimizing enzyme inactivation. The optimum conditions turned out to be: Tris buffer concentration 30 mM, pH 8.0, contact time at room temperature 3 hr, and enzyme loading 25 mg protein/g resin. Both the thermal stability and the operational stability of immobilized enzyme were markedly enchanced by the treatment with 0.5% glutaraldehyde as a cross-linker. Under the experimental conditions established, the yield of ${\beta}-galactosidase$ immobilization was 40% or more and the activity of the immobilized enzyme ca. 200 U/g resin. When a packed-bed reactor was employed to continuously convert lactose to GOS, the specific production, which refers to as the amount of commercially valuable GOS produced by a unit amount of immobilized ${\beta}-galactosidase$, was found to be ca. 300 g GOS/g carrier.

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Preprocessing-based speed profile calculation algorithm for radio-based train control (무선통신기반 열차간격제어를 위한 전처리 기반 속도프로파일 계산 알고리즘)

  • Oh, Sehchan;Kim, Kyunghee;Kim, Minsoo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.9
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    • pp.6274-6281
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    • 2015
  • Radio-based train control system has driving headway shortening effect by real-time train interval control using two-way radio communication between onboard and wayside systems, and reduces facility investment because it does not require any track-circuit. Automatic train protection(ATP), the most significant part of the radio-based train control system, makes sure a safe distance between preceding and following trains, based on real-time train location tracing. In this paper, we propose the overall ATP train interval control algorithm to control the safe interval between trains, and preprocessing-based speed profile calculation algorithm to improve the processing speed of the ATP. The proposed speed profile calculation algorithm calculates the permanent speed limit for track and train in advance and uses as the most restrictive speed profile. If the temporary speed limit is generated for a particular track section, it reflects the temporary speed limit to pre-calculated speed profile and improves calculation performance by updating the speed profile for the corresponding track section. To evaluate the performance of the proposed speed profile calculation algorithm, we analyze the proposed algorithm with O-notation and we can find that it is possible to improve the time complexity than the existing one. To verify the proposed ATP train interval control algorithm, we build the train interval control simulator. The experimental results show the safe train interval control is carried out in a variety of operating conditions.

Removal of Ammonia Nitrogen and Organics from Piggery Wastewater Using BACC Process-I. Comparison of Activated Sludge Process (BACC를 이용한 축산폐수의 암모니아성 질소 및 유기물의 제거 I. 활성슬러지 공정과의 비교)

  • 성기달;류원률;김인환;조무환
    • KSBB Journal
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    • v.16 no.2
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    • pp.133-139
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    • 2001
  • To treat piggery wastewaters containing refractory compounds including nitrogen, biological treatments were investigated. In biological treatment, the removal efficiencies of organics and nitrogen by the activated sludge process and bioreactor using a BACC (Biological Activated Carbon Cartridge) media filled with granular activated carbon were examined. The results were as follows; in the biological process, when the approximate influent BOD concentration of 620 mg/L, through dilution, was treated by the activated sludge process, the process should be operated at a HRT of over 8 days to maintain an effluent BOD concentration of lower than 100 mg/L. In the treatment of piggery wastewater using a BACC bioreactor, when the HRT was 200 hours, the BOD, COD(sub)cr, and TKN removal efficiency of the effluent were 94, 75 and 64.3%, respectively. Comparing the BACC bioreactor with the activated sludge process, when the volumetric loading rate was 0.3 g BOD/L.day, the specific substrate removal rate of BOD was 0.14 g BOD removed/L.day in the activated sludge process which compared with 0.27 g BOD removed/L$.$day in the BACC bioreactor. The BACC bioreactor showed on average a 2-fold higher removal rate and was superior to the activated sludge process in wastewater treatment in terms of variations of loading time and high loading time. Therefore, the BACC process can effectively treat piggery wastewater containing high concentrations of nitrogen and organic compounds.

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Membrane Fouling Control Effect of Periodic Water-back-flushing in the Tubular Carbon Ceramic Ultrafiltration System for Recycling Paper Wastewater (제지폐수 재활용을 위한 관형 탄소계 세라믹 한외여과장치에서 물 역세척의 막오염 제어 효과)

  • 김미희;박진용
    • Membrane Journal
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    • v.11 no.4
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    • pp.190-203
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    • 2001
  • In this study the discharged wastewater from a paper plant was filtrated by 4 kinds of tubular carbon ceramic ultrafiltration membranes with periodic water-back-flushing. We could investigate effects of watch-back-flushing period, transmembrane pressure (TMP) and flow rate, and find optimal operating conditions. The back-f1ushing time (BT) was fixed at 3 sec, and fi1tration times (FT) werc changed in 15~60 scc, TMP in 1.00~2.50$kg_{f}$/$cm^2$, and the flow rates in 0.27~1.75 L/min. The optimal conditions were discussed in 7he viewpoints of dimensionless permeate flux (J/J$_{0}$), total permeate volume ($V^T$) and resistance of membrane fouling ($R^f$). Optima1 back-flushing period was BT/FT=0.20, suggesting that the frequent back-flushing should decrease membrane fouling. Optimal TMP in the viewpoint of $V^T$ was 1.00~1.55$kg_{f}$/$cm^2$, suggesting that rising TMP should increase membrane fouling and decrease permeate flux. But, rising f1ow rate should decrease membrane fouling and increase permeate flux. Then, average rejection rates of pollutants filtratedby carbon ceramic membranes were 88~98 % for turbidity, 48~72% fort $COD_{cr}$ and 37~76% for TDS.

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Effect of Periodic $N_2$-back-flushing in Paper wastewater Treatment using Carbon Ceramic Ultrafiltration and Microfiltration Membranes (탄소계 세라믹 한외 및 정밀 여과막으로 제지폐수 처리시 주기적 질소 역세척의 효과)

  • 황현정;박진용
    • Membrane Journal
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    • v.12 no.1
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    • pp.8-20
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    • 2002
  • In this study using $N_2$-back flushing, which wwas not the general back-flushing method of membranes, the discharged wastewater from a paper plant was filtrated by 4 kinds of tubular carbon ceramic ultrafiltration membranes. We could in vestigate effects of $N_2$-back flushing period, transmembrane pressure (TMP)and flow rate and find optimal operating conditions. The $N_2$-back flushing time (BT) was fixed at 40 sec, filtration times (FT) were changed in 4~32 min, TNP in $1.0~3.0kg_f/cm^2$ the flow celocities in 0.53~1.09cm/s. The optimal conditions were discussed in the viewpoints of dimensionless permeate flux ($J/J_0$), toal permeate volume ($V_T$) and resistance of membrane fouling ($R_f$). Optimal back-flushing period was BT/FT=0.167 (FT=8 min ), in which more $V_T$ was obtained than that in BT/FT=0.083 (FT=4 min) which was the most friquent back-flushing condition. Then rising TMP should increase the driving force, and more $V_T$ could be accumulated. And rising flow rate should decrease membrane fouling increase permeate flux, and more $V_T$could be produced. Average rejection rates of pollutants were higher than 95% for turbidity and 45~83% for $COD_{Cr}$, but rejection rates of total dissolved solid (TDS) were lower than 10%.

Productivity Changes by Public Transport Reforms in the Seoul's Urban Bus Industry (Using Data Envelopment Analysis with Consideration of Accident Costs) (서울의 대중교통체계 개편에 따른 시내버스업체의 생산성 변화 (사고비용을 고려한 자료포락분석기법을 이용하여))

  • Oh, Mi-Young;Kim, Sung-Soo
    • Journal of Korean Society of Transportation
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    • v.26 no.4
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    • pp.77-86
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    • 2008
  • Firstly, this study is to compare two types of productivity changes in Seoul's urban bus industry by public transport reforms: one with considering accident costs (ML), the other without considering accident costs (M). The directional distance function (DDF), which is a form of data envelopment analysis, and the Malmquist (Luenberger) productivity index (ML, M) are used for the analysis. The results show that productivity increased regardless of accident costs over time, and the ML index is a little higher than the M index, which means that most firms experienced more of a reduction ratio of accident costs than a reduction ratio of inputs. Secondly, this paper is to analyze how reforms affect productivity changes. The results show that operational speed improvements or passenger increases most affect productivity increases, which means that the installment of median bus lanes or introduction of free transfers among public transport modes were effective in reducing both inputs and accident costs. However, operational system reforms including mid-public management were related with productivity changes but they were not as effective.

Smart Synthetic Path Search System for Prevention of Hazardous Chemical Accidents and Analysis of Reaction Risk (반응 위험성분석 및 사고방지를 위한 스마트 합성경로 탐색시스템)

  • Jeong, Joonsoo;Kim, Chang Won;Kwak, Dongho;Shin, Dongil
    • Korean Chemical Engineering Research
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    • v.57 no.6
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    • pp.781-789
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    • 2019
  • There are frequent accidents by chemicals during laboratory experiments and pilot plant and reactor operations. It is necessary to find and comprehend relevant information to prevent accidents before starting synthesis experiments. In the process design stage, reaction information is also necessary to prevent runaway reactions. Although there are various sources available for synthesis information, including the Internet, it takes long time to search and is difficult to choose the right path because the substances used in each synthesis method are different. In order to solve these problems, we propose an intelligent synthetic path search system to help researchers shorten the search time for synthetic paths and identify hazardous intermediates that may exist on paths. The system proposed in this study automatically updates the database by collecting information existing on the Internet through Web scraping and crawling using Selenium, a Python package. Based on the depth-first search, the path search performs searches based on the target substance, distinguishes hazardous chemical grades and yields, etc., and suggests all synthetic paths within a defined limit of path steps. For the benefit of each research institution, researchers can register their private data and expand the database according to the format type. The system is being released as open source for free use. The system is expected to find a safer way and help prevent accidents by supporting researchers referring to the suggested paths.

The Risk Assessment of Carbon Monoxide Poisoning by Gas Boiler Exhaust System and Development of Fundamental Preventive Technology (가스보일러 CO중독 위험성 예측 및 근원적 예방기술 개발)

  • Park, Chan Il;Yoo, Kee-Youn
    • Journal of the Korean Institute of Gas
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    • v.25 no.3
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    • pp.27-38
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    • 2021
  • We devised the system to automatically shutdown the boiler and to fundamentally block the harmful gases, including carbon monoxide, into the indoor when the exhaust system swerves: (1) The discharge pressure of the exhaust gas decreases when the exhaust pipe is disconnected. The monitoring system of the exhaust pipe is implemented by measuring the output voltage of APS(Air Pressure Sensor) installed to control the amount of combustion air. (2) The operating software was modified so that when the system recognizes the fault condition of a flue pipe, the boiler control unit displays the fault status on the indoor regulator while shutting down the boiler. In accordance with the ventilation facility standards in the "Rules for Building Equipment Standards" by the Ministry of Land, Infrastructure and Transport, experiments were conducted to ventilate indoor air. When carbon monoxide leaked in worst-case scenario, it was possible to prevent poisoning accidents. However, since 2013, the number of indoor air exchange times has been mitigated from 0.7 to 0.5 times per hour. We observed the concentration exceeding TWA 30 ppm occasionally and thus recommend to reinforce this criterion. In conclusion, if the flue pipe fault detection and the indoor air ventilation system are introduced, carbon monoxide poisoning accidents are expected to decrease significantly. Also when the manufacturing and inspection steps, the correct installation and repair are supplemented with the user's attention in missing flue, it will be served to prevent human casualties from carbon monoxide poisoning.

Crystal Structure Behavior of Vanadium-Titanium Magnetite (VTM) Ore by Planetary Ball Mill (바나듐 함유 티탄철광의 유성 볼밀에 의한 결정구조 거동)

  • Han, Yosep;Kim, Seongmin;Jung, Minuk;Jeon, Ho-Seok
    • Resources Recycling
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    • v.31 no.2
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    • pp.63-69
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    • 2022
  • In this study, mechanical grinding using a planetary ball mill was performed under various conditions to evaluate its effect on the crystal structure of vanadium titanium magnetite (VTM) ore from the Kwain Mine in South Korea. The crystal structure of the activated product was also evaluated. Magnetite and ilmenite were identified as the main types of VTM ore used in the Kwain Mine, and the main types of gangue minerals were iron-based silicate minerals. According to the mechanical activation results, the crystallinity and crystal size decreased as the size of the grinding media (balls) decreased, and the amorphization of the sample/ball filling was significant as the amount of the sample was reduced. In addition, as the grinding speed and time increased, the crystal structure significantly changed, proving that these two parameters had a greater effect on the crystal structure than the ball size and sample/ball filling ratio.

Training of a Siamese Network to Build a Tracker without Using Tracking Labels (샴 네트워크를 사용하여 추적 레이블을 사용하지 않는 다중 객체 검출 및 추적기 학습에 관한 연구)

  • Kang, Jungyu;Song, Yoo-Seung;Min, Kyoung-Wook;Choi, Jeong Dan
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.21 no.5
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    • pp.274-286
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    • 2022
  • Multi-object tracking has been studied for a long time under computer vision and plays a critical role in applications such as autonomous driving and driving assistance. Multi-object tracking techniques generally consist of a detector that detects objects and a tracker that tracks the detected objects. Various publicly available datasets allow us to train a detector model without much effort. However, there are relatively few publicly available datasets for training a tracker model, and configuring own tracker datasets takes a long time compared to configuring detector datasets. Hence, the detector is often developed separately with a tracker module. However, the separated tracker should be adjusted whenever the former detector model is changed. This study proposes a system that can train a model that performs detection and tracking simultaneously using only the detector training datasets. In particular, a Siam network with augmentation is used to compose the detector and tracker. Experiments are conducted on public datasets to verify that the proposed algorithm can formulate a real-time multi-object tracker comparable to the state-of-the-art tracker models.