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Protease Activity of Lactic Acid Bacteria Isolated from Korean Traditional Fermented Food (전통 발효식품으로부터 Protease 활성을 보유한 유산균의 분리 및 동정)

  • Kook, Moo Chang;Cho, Seok Cheol;Park, Hoon;Kim, Seung Seop;Pyun, Yu Ryang;Choi, Woon Yong;Lee, Hyeon Yong
    • Food Engineering Progress
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    • v.15 no.2
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    • pp.182-187
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    • 2011
  • A proteolytic lactic acid bacterium was isolated from Korean traditional fermented foods. The isolate BV-26, which had a protease activity (24 U/mg-crude protein), was identified as Lactobacillus plantarum by the API 50CHL kit and 16S rDNA analysis (99.9% of homology), and named as L. plantarum BV-26. Cell growth and protease activity of L. plantarum BV-26 was determined in MRS broth using 5L jar fermentor at $30^{\circ}C$. The maximum growth of L. plantarum BV-26 was reached at 18 hr in MRS broth, while protease activity of BV-26 was detectable at 12 hr and the highest activity was obtained after 16 hr cultivation. Therefore, we expect that the proteolytic lactic acid bacteria, L. plantarum BV-26, may be used as a starter for the fermentation of animal feed. Especially, the fermentation of soybean meal with the strain can be applied for improving feed utilization.

A study on the selection of candidates for public bases according to the spatial distribution characteristics Automated External Defibrillator in Daegu City (대구시 자동심장충격기 공간분포 특성에 따른 공공 거점후보지 선정 연구)

  • Beak, Seong Ryul;Kim, Jun Hyun
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.38 no.6
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    • pp.599-610
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    • 2020
  • The AED (Automated External Defibrillator) is not evaluated for spatial accuracy and temporal availability even if it is located within a building or a specific area that needed necessary to partition by spatial analysis and location allocation analysis. As a result of the analysis, the spatial analysis was performed using the existing public data of AED with applied the GIS location analysis method. A public institution (119 safety center, police box) was selected as a candidate for a public AED base that can operate 24 hours a day, 365 days a year according to the characteristics of each residential area. In addition, Thiessen Polygons were created for each candidate site and divided by regions. In the analysis of the service was analyzed regional in terms of accessibility to emergency medical services in consideration of the characteristics of AED, that emergency vehicles could arrive within 4 minutes of the time required for emergency medical treatment in most areas of the study area, but it did not areas outside of the city center. As a result, It was found that the operation of the AED base service center centered on vehicles of public institutions is effective for responding to AED patients at night and weekend hours. 19 Safety Center under and police box the jurisdiction of Daegu City to establish an AED service center for public institutions, location-based distance, attribute analysis, and minimization of overlapping areas that the method of using a vehicle appeared more efficient than using the existing walking type AED.

Antioxidant and Anti-inflammatory Activities in Freeze-dried and Hot Air-dried Aronia (Aronia melanocarpa) Extracts (동결 및 열풍건조 아로니아 추출물의 항산화 및 항염증 활성)

  • Park, Mi-Hye;Kim, Bumsik
    • Food Engineering Progress
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    • v.22 no.4
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    • pp.315-320
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    • 2018
  • This study examined the effects of drying methods of Aronia melanocarpa on contents of total polyphenols and flavonoids, antioxidant activity and anti-inflammatory activity. As a result, freeze-dried aronia extract (FDAE) significantly exhibited higher contents of total polyphenols and flavonoids (155.76 mg GAE/g and 105.70 mg QE/g) than hot-air dried aronia extract (HDAE) (134.93 mg GAE/g and 82.29 mg QE/g). Also, FDAE showed greater antioxidant activity than HDAE in both DPPH and ABTS. For anti-inflammatory activity, NO production from lipopolysaccharide activated RAW264.7 cell reduced at a dose-dependent manner in both FDAE and HDAE. However, reduction rate of NO production is higher in FDAE (62.7%) than in HDAE (33.5%). These results suggest that comparatively, freeze-drying is a better method for preserving the antioxidant and anti-inflammatory activities and of aronia.

A Study on the Applicability of Air Launch Vehicle (공중발사체의 활용가능성 분석 연구)

  • Kwon, Kybeom;Lee, Kanghyun;Cho, Ye Rang;Ji, Wan Gu;Kim, Kyu Hong
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.50 no.3
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    • pp.203-214
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    • 2022
  • As the global demand for small satellites weighing less than 500 kg increases, the development and operation of dedicated small launch vehicles increase significantly. The responsiveness of a launch vehicle that puts a small satellite into a target orbit at the desired time is attracting attention. As a result, interest in the air launch is increasing in the rapid establishment of a constellation. As the demand for small satellites in south Korea increases, this study performed analyses on the applicability of an air launch vehicle using a large civil aircraft considering the geographical environment. In terms of responsiveness, mission response times were compared and analyzed for air launch vehicles and ground small and large vehicles. In addition, an air vehicle and a small ground vehicle were quantitatively compared and analyzed for the orbital insertion performance. As a result of the analysis, the air launch vehicle has limited responsiveness in Korea regarding rapid satellite constellation establishment. However, it can be an effective alternative for low inclination angle orbit insertion with the benefit of a fast turnaround time. Furthermore, the performance of the orbital injection is close to that of the ground small launch vehicle, and the high efficiency in terms of the required propellant mass is possible, so air launch can be an effective launch means for putting small satellites into orbit in Korea.

Change of Predator Recognition Depends on Exposure of Predation Risk Source in Captive Breed Endangered Freshwater Fish, Microphysogobio rapidus (인공증식된 멸종위기종 여울마자의 포식 위험원 노출에 따른 포식자 인지 변화)

  • Moon-Seong Heo;Min-Ho Jang;Ju-Duk Yoon
    • Korean Journal of Ecology and Environment
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    • v.56 no.4
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    • pp.406-413
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    • 2023
  • Captive breeding and reintroduction are crucial strategies for conserving endangered species populations. However, fish raised in predator-free environments, show a lack of recognition of predationrelated stimuli such as chemical and visual signals. It is critical to recognize chemical signals from injured conspecifics, also known as alarm signals, and the order or shape of predators to indicate the spread of predation risk in the habitat. We conducted a laboratory experiment to determine and adjust the optimal exposure period to induce appropriate anti-predator behavior response to different types of stimuli (Chemical, Visual and Chemical+Visual) for the endangered species Microphysogobio rapidus. Our results demonstrate that predator avoidance behavior varies depending on the types of stimuli and the duration of predation risk exposure. First, the results showed captive-breed M. rapidus show lack of response against conspecific alarm signal (Chemical cue) before the predation risk exposure period and tend to increase response over predation risk exposure time. Second, response to predator (visual cue) tend to peak at 48 hours cumulative exposure, but show dramatic decrease after 72 hours cumulative exposure. Finally, response to the mixed cue (Chemical+visual) tend to peak prior to the predation risk exposure period and show reduced response during subsequent exposure periods. This experiment confirms the lack of responsiveness to conspecific alarm signals in captive-bred M. rapidus and the need for an optimal nature behavior enhancement program prior to release of endangered species. Furthermore, responsiveness to predator visual signal peak at 48 hours cumulative exposure, suggest an optimal predation risk exposure period of up to 48 hours.

A Study on the Water Exchange Plan with Disaster Prevention Facilities in Masan Bay (마산만 재해방지시설을 이용한 해수교환 방안에 관한 연구)

  • Kim, Gweon-Su;Ryu, Ha-Sang;Kim, Kang-Min
    • Journal of Navigation and Port Research
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    • v.37 no.6
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    • pp.637-645
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    • 2013
  • Masan bay with a semi-enclosed waters has serious water quality problems due to the low flow and river pollution load from land, and shows the vulnerable locational characteristics to storm surge. We are seeking the way of both operating disaster prevention facilities and water quality improvement measures in the bay. That is, the water was exchanged using the head difference occurred by operating disaster prevention facilities. The location of disaster prevention facilities was assumed to be in the inlet of the bay, in the vicinity of Machang bridge, and in the vicinity of Dot island and the operation time was assumed to be early morning hours(01~05) considering the number of shipping passage and annual tide, and spring tide of the largest head difference. In addition, the experiment case of water exchange including the in-outflow feeder pipe was tested. According to the simulation results, water exchange rate in all experiments has shown a steady increase. Water exchange rate of the whole of Masan bay in the case of present is 38.62%. The water exchange rate of the inside of Masan bay compared with the inlet of bay, appeared to be very low. Thus, we judged that the characteristics of semi-enclosed waters were well reproduced. On the results of the experiment of disaster prevention facilities and in-outflow feeder pipe, the case of the operation of disaster prevention facilities, water exchage rate is high compared with the case of present. And, the higer the operating frequency, the more water exchange is appeared. The cases of water exchange prevention facilities through the in-outflow feeder pipe caused by the head difference, also showed the higest improvement of the water quality. Compared with the south of Machang bridge, the effect of water exchange was better in the inlet of Masan bay and Dot island. On the other hand, the inlet of Masan bay is higer than Dot island as for water exchange of the whole of Masan bay, but opposite, water change rate including Masan inside was higher in the case of Dot island.

Monte Carlo Simulation based Optimal Aiming Point Computation Against Multiple Soft Targets on Ground (몬테칼로 시뮬레이션 기반의 다수 지상 연성표적에 대한 최적 조준점 산출)

  • Kim, Jong-Hwan;Ahn, Nam-Su
    • Journal of the Korea Society for Simulation
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    • v.29 no.1
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    • pp.47-55
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    • 2020
  • This paper presents a real-time autonomous computation of shot numbers and aiming points against multiple soft targets on grounds by applying an unsupervised learning, k-mean clustering and Monte carlo simulation. For this computation, a 100 × 200 square meters size of virtual battlefield is created where an augmented enemy infantry platoon unit attacks, defences, and is scatted, and a virtual weapon with a lethal range of 15m is modeled. In order to determine damage types of the enemy unit: no damage, light wound, heavy wound and death, Monte carlo simulation is performed to apply the Carlton damage function for the damage effect of the soft targets. In addition, in order to achieve the damage effectiveness of the enemy units in line with the commander's intention, the optimal shot numbers and aiming point locations are calculated in less than 0.4 seconds by applying the k-mean clustering and repetitive Monte carlo simulation. It is hoped that this study will help to develop a system that reduces the decision time for 'detection-decision-shoot' process in battalion-scaled combat units operating Dronebot combat system.

A Development of Navigation Routes Recommendation System with Elements Analysis of Marine Leisure Activities (해양 레저 활동을 위한 요소 분석 및 항로 추천 시스템의 개발)

  • Kim, Bae-Sung;Hwang, Hun-Gyu;Shin, Il-Sik;Lee, Jang-Se;Yoo, Yung-Ho
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.7
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    • pp.1355-1362
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    • 2016
  • Recently, the marine leisure are being emphasized with improving the quality of life style by increased income and spare time. Also, there is a increasement of people's interest in marine leisure activities. But resources and facilities do not grow in proportion to the quantitative growth of the current marine leisure industry. Besides, a leisure ship operator tends to choose a simple or familiar route of the local area rather than a new leisure routes which are not explored due to lack of accessible areas information. This paper proposes a routes recommendation system in order to solve above problems based on marine resource database. The databases have been constructed through investigation and analysis of navigational information such as environmental conditions including weather conditions and sea status, field of marine leisure activities, tourist attractions and natural landscape, and marine leisure prohibited areas. Therefore we have developed and implemented the route recommendation system that provides various information necessary to route operation of leisure boats.

A Study on the Problems in the Use of CCTV by the Police and Some Proposals (경찰CCTV 운용상의 문제점과 개선방안)

  • Lee, Sang-Won;Lee, Seung-Chal
    • Korean Security Journal
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    • no.10
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    • pp.215-242
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    • 2005
  • As CCTV can be an effective tool to prevent or suppress crime at low cost, they have been widesoread in developed countries. In spite of their effectiveness, they infringe some constitutional rights such as the right to privacy, the right of likeness and the right to control over personal information. The police and ward offices install CCTV in public areas to prevent crimes without a legal basis or standard. When information obtained in such a way is used as investigation data for the police or as an evidence in a court, it can cause serious trouble. To solve this problem, legal restriction on the installation of CCTV as should be clearer. Since current laws on public agencies' protection of personal information are too general, they are not effective enough to protect personal information. Therefore, Personal Information Protection Organic Act should be enacted to make a legal basis for protecting comprehensive personal information. It should be obvious who installs CCTVs, who pay for the cost and how they are managed. Before installation, the police and ward offices should obtain residents' consent through a public hearing or voting (on the range and purpose of installation), or conduct an impact assessment. During installation, CCTVs should be limited to prevent or suppress crimes, keep public order and void dangers. In case of making a sign of installation, it must specify its rights. After installation(operation/management phase), they should abide by principles of information protection and try not to infringe constitutional right. In the cognitive aspect, the police should constitutional rights must be secured although it is important to carry out their missions. The police should serve citizens and change to the police of communities. Citizens should understand that constitutional right can be infringed if public order is not maintained. When citizens cooperate with the police, they fear of crimes will decrease.

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An Effective Employment and Execution Performance Improvement Method of Mobile Web Widget Resources Based on the OMTP BONDI (OMTP BONDI 기반 모바일 웹 위젯 리소스의 효율적 운용 및 구동 성능 개선 기법 연구)

  • Bang, Ji-Woong;Kim, Dae-Won
    • Journal of Korea Multimedia Society
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    • v.14 no.2
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    • pp.153-170
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    • 2011
  • OMTP (Open Mobile Terminal Platform) is a global forum made by telecommunications providers to promote user-oriented mobile services and data business. Devised by OMTP, BONDI is a browser-based application or a mobile web run-time platform to help widgets make good use of functions of mobile devices in a secure way. BONDI enables applications programmed with web standard technologies such as HTML, JavaScript, CSS, and AJAX to reach the internal functions of mobile devices. Since BONDI, which is not just a simple network application, can reach the internal resources of devices in standard ways, it enables the application and widgets to be developed regardless of tile OS or platform. Web browser-based widgets are vulnerable to the network environment, and their exeeution speed can be slowed as the operations of the widgets or applications become heavy. However, those web widgets will be continuously used thanks to the user-friendly simple interface and the faster speed in using web resources more than the native widgets inside the device. This study suggested a method to effectively operate and manage the resource of OMTP BONDI web widget and then provided an improved result based on a running performance evaluation experiment. The experiment was carried to improve the entire operating time by enhancing the module-loading speed. In this regard, only indispensable modules were allowed to be loaded while the BONDI widget was underway. For the purpose, the widget resource list, able to make the operating speed of the BONDI widget faster, was redefined while a widget cache was employed. In addition, the widget box, a management tool for removed widgets, was devised to store temporarily idle widgets.