• Journal of the korean academy of Pediatric Dentistry
• /
• v.29 no.2
• /
• pp.270-277
• /
• 2002

Since founded in 1959, it's well known that the KAPD has pioneered in the researches and clinical aspects of pediatric dentistry in Korea. It's official journal, the Journal of the KAPD, was first published in 1974 and has pressed total 956 articles up to now(March, 2001). In this study, all the articles pressed in this journal have been surveyed, focussing in their main theme, their chronological and thematic distribution. The thematic classification was made with the reference of the previous studies and renowned textbooks in pediatric dentistry. And we obtained the results as follows: 1. The researches on dental materials and dental equipments have shown continuous increase throughout the period. 2. The researches on dental caries, caries prevention and systemic disorders have occupied relatively high proportion consistently. 3. The researches on malocclusions and cysts/minor surgery have shown increasing tendency in the second period, but are decreasing in the third period. 4. The researches on craniofacial growth/development, tooth development/eruption, developmental disorders of teeth, management of eruption space have shown decreasing tendency. 5. The researches on behavioral research, oral habits, occlusion of primary-mixed dentition have shown very low proportion, reaching no more than 1% throughout the period.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.32 no.2
• /
• pp.357-369
• /
• 2005

Streptococci are Gram-positive, facultative anaerobes and have no catalase activities. Among mutans streptococci containing ${\alpha}-type$ hemolytic activity, S. mutans is a causative agent for dental caries. As well as acid production yielding the demineralization of tooth enamel, adherence and colonization of S. mutans to the teeth are also important for its virulence. These early colonization are accomplished by the bacterial fibrillar protein, Antigen I/II (Ag I/II) and glucosyltransferase (GTF). Therefore, Ag I/II and GTF are reasonable targets for the development of vaccine against S. mutans GS-5. The ag I/II and gtfD genes from S. mutans GS-5 were cloned and sequenced. Sequence analyses showed the nucleotides sequence of cloned genes had high homology to the sequences previously reported. The sequence alignment of 280 nucleotides between the cloned Ag I/II and the available sequence of the corresponding S. mutans GS-5 showed the perfect match. Comparing with the sequence of gtfD from S. mutans UA159, the corresponding nucleotide sequence of S. mutans GS-5 showed some mismatches and the mismatches introduced changes in four residues out of 105 amino acids, yielding four missense mutations.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.34 no.1
• /
• pp.53-61
• /
• 2007

In case of missing tooth caused by dental caries or periodontal disease, it can be restored by various methods, and there has been much interest in implant and tooth transplantation. The success of tooth transplantation is going to be attained through the knowledge of growth, development and calcification of tooth. Tooth transplantation has been experimented in vivo and in vitro. Many animals such as rats, mice, cats and dogs are used for tooth transplantation experiment in vivo. In most experiments, tooth was transplanted into the extraoral site, but rare into the intraoral site In this study, to observe the capacity of formation and mineralization of tooth germ, first molar of a matured white rat was extracted and the cap stage tooth germ of a 13.5 Embryonic day embryo rat was transplanted into the extracted socket. The rats were killed 6 months later and the radiographical and histological results are as followings. 1. Tooth germ transplanted for 2 and 6 months are developing calcified tooth material such as dentin, cementum, pulp tissue, and epithelium around enamel space in the maxilla was seen. 2. The epithelium around enamel space was located beneath the oral epithelium and contained connective tissue and periodontal ligament. 3. Tooth formation was progressed as transplantation period but the size of newly formed tooth was small and the shape of tooth was incomplete.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.38 no.4
• /
• pp.317-326
• /
• 2011

QLF-D system composed with DSLR(digital single-lens reflex) camera, and the images of natural enamel caries and artificial caries was developed from 2 days to 14 days captured by QLF-D system. The correlation between lesion depth of the polarized microscope and luminosity ratio of QLF-D image was analyzed and the results were summarized as follows: 1. The Pearson correlation value between the lesion depth of polarized microscope images and luminosity ratio of QLF-D images was 0.969(p<0.01). 2. From Regression analysis of lesion depth from polarized image by demineralized period, the equation was y = 8.67x - 1.16(p<0.05). 3. From Regression analysis of luminosity ratio from QLF-D image by demineralized period, the equation was y = 3.53x + 6.42(p<0.05). From the results, QLF-D system can detect the enamel caries at the very early stage and can monitor the progression of demineralization and remineralization. For the convenient use of QLF-D system in the laboratory, the image analysing software was needed to analyze of interest site of enamel caries lesion.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.38 no.1
• /
• pp.9-16
• /
• 2011

Recently, the number of multi-cultural families have been on the rise for years in Korea. Although social concerns are on the increase, there is still no basic data of their oral health state. In this study, we examined the caries prevalence of mother-child pairs from some korean families and multi-cultural families, and collected some saliva from them. We evaluated Streptococcus mutans level and investigated the serotype distribution of Streptococcus mutans of them to confirm the transmission in mother-child pairs. As a results, to the Korean families, the percentages of serotype c, e, f, k were 95%, 10%, 10%, and 10%. And to the multi-cultural families, the percentages of serotype c, e, f, k were 68.8%, 31.25%, 43,75%, 18.8%. But, there is no significant relationship between the number of serotype per person, caries prevalence, and Streptococcus mutans level(p>0.05). In conclusion, there are differences of serotype distribution of Streptococcus mutans between the two families. Nevertheless, matching rate of more than one serotype between mother and their child was high (92.86%). So, vertical transmission of Streptococcus mutans in mother-child pairs could be demonstrated.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.37 no.3
• /
• pp.345-351
• /
• 2010

The purpose of this study is to compare microleakage and penetration depths of sealants applied in carious and sound fissures. Extracted premolars(n=80) were divided into 4 groups according to caries status. 1; sound, 2; stained, 3; initial caries, 4; enamel caries. Sealants were applied to the occlusal groove as per manufacturers' instructions, and specimens were thermocycled, stained, sectioned, and examined for microleakage and penetration ability. The results of the present study are as follow: 1. It showed significantly higher microleakage scores in group I than group Two, Three, Four(p<0.05), but no significant difference was found between group Two, Three, and IV(p>0.05). 2. It showed significantly higher penetration scores in group Four (p<0.05), followed by group One, Two, Three, but no significant difference was found between group One, Two, and Three(p>0.05). Based on the results of present study, when the borders of the fissure sealant are on carious enamel, a significantly higher microleakage must be expected. It is considered that depth of enamel caries in the fissure should be taken into account when applying a fissure sealant.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.37 no.3
• /
• pp.352-358
• /
• 2010

Chemotherapy or radiotherapy used for the treatment of pediatric cancer may have many adverse effects on the oral cavity. Oral mucositis, reduced salivary flow, oral infection, hypodontia, microdontia, arrested root development, and enamel hypoplasia are common oral complications. The aim of this study is to evaluate the effects of cancer therapy on dental caries activities. The children who had been treated for neuroblastoma in the department of pediatrics, Samsung Medical Center, were included and healthy children served as controls. The salivary flow rate, salivary buffering capacity, and Streptococcus mutans counts of both groups were evaluated using Dentocult$^{(R)}$ SM and Dentobuff$^{(R)}$ Strip. The dental caries activity related to the age at the start of treatment and the time elapsed since treatment completion were also evaluated. As a result, neuroblastoma patients had significantly lower salivary flow rate than the controls, while there were no significant differences between two groups as for salivary buffering capacity and Streptococcus mutans counts. The dental caries activities related to the age at the start of treatment and the time elapsed since treatment completion were not significantly different.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.37 no.3
• /
• pp.275-287
• /
• 2010

Deep caries may induce pulpitis and the pulpal tissue interacts with microbial invasion. The immune response to protect the pulpal tissue can be mediated by cellular signal molecules produced by the pulpal cells. The understanding of these processes is important to find future therapeutic method for the diseased pulp. The pulp tissue from sound teeth was set as control group (n=30) and the pulp tissue from decayed teeth was set as test group (n=30). Total RNA was extracted from the pulp of each group and it was used for cDNA microarray and reverse transcriptase-polymerase chain reaction(RT-PCR). The expression of TGF-${\beta}1$ was studied by immunohistochemistry. The results were as follows: 1. cDNA microarray analysis identified 520 genes with 6-fold or greater difference in expression level with 143 genes more abundant in health and 377 genes more abundant in disease. 2. The RT-PCR analysis was done for randomly selected 14 genes and the results supported the result of cDNA microarray assay. 3. TGF-${\beta}1$ was highly expressed in the carious pulp and it was found in odontoblast by immunohistochemistry. In conclusion, many cytokines were found to be significantly changed their expression in the diseased pulp(/M/>1.6).

• Journal of dental hygiene science
• /
• v.4 no.1
• /
• pp.39-44
• /
• 2004

The primary purpose of this report is to investigate the index of dental caries experiences and the state of oral hygiene for the children with mental retardation, cerebral palsy, and autism. In order to get those things we investigated 99 students that ages from 8 to 13 in three special schools (which are the school for the children with mental retardation, physical difficulties, emotional disturbance). Then collected the statistics and examined if there were some regardful differences among each type. (1) The investigation shows that there is no regardful differences among them in statistice. However, the averages of their dental caries experience have a little gap. The average of the children with mental retardation group is the highest and the autistic children group is the lowest, as we can see the facts of 4.70 for group of children with mental retardation, 4.58 for group of children with cerebral palsy, 3.67 for group of autistic children, children with mental retardation group is severe, autistic children group is few. (2) The conclusion from research of oral hygiene among each type, statistically regardful differences were revealed (p<0.05). It shows children with cerebral palsy is on worst state, children with mental retardation is on next state, autistic children is on best state by compare for averages among each type, 32.30 for group of children with mental retardation 35.00 for group of children with cerebral palsy, 27.79 for autistic children.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.23 no.2
• /
• pp.415-427
• /
• 1996

As a part of host factors of dental caries, saliva has been well known for its important role in relation to dental caries. The studies on its physical and chemical characteristics on development and progress of dental caries has been conducted. Recently, various comparisons between saliva of caries-susceptable individuals and caries-free individuals has been done and the efforts to understand the mechanisms of salivary intervention of development and progress of dental caries is actively in progress. In this study, 15 children with rampant dental caries and 15 caries free children without any systemic diseases from the ages of 2 to 5 were chosen for the experiment and the whole saliva and parotid saliva from each individuals were collected and protein compositions were compared using polyacrylamide gel electrophoresis (PAGE). As results of this study, in parotid saliva, there was no difference in protein compositions between the rampant dental caries and the caries free children. While electrophoresis was done with the whole saliva, protein with 120 KDa was found in children with rampant dental caries. However, this protein was not found or unclear, if any for the caries free group. (Exceptionally, clear protein band was present for one person.) Protein compositions of whole saliva of rampant dental caries group was compared before and after the caries control and thick and clear protein bands of about 120 KDa were found in both cases. Protein compostions of caries free children and adults were identical. Quantitative analysis of protein was done for the rampant dental caries group and the control group and no significant difference was found. Taken all together, protein with molecular weight of 120 KDa, found in rampant dental caries group, was still present when the treatment for the dental caries was done so it can be assumed that this protein has no interrelation with the presence of active carious lesions during saliva collecting. It can also be presumed that this specific salivary protein with the molecular weight of 120 KDa found in rampant dental caries group has effect on development and progress of dental caries. Identification on this protein with the molecular-weight of 120 KDa and the role of this protein against dental caries remain to be solved.