• Title/Summary/Keyword: 용균

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Properties of Soil Suppressiveness to Cucumber Wilt, caused by Fusarium oxysporum f. sp. cucumerinum Owen (오이 덩굴쪼김병의 발병(發病)을 억제(抑制)하는 토양(土壤)의 특성(特性)에 관(關)하여)

  • Park, Chang-Seuk;Cho, Yong-Sup
    • Korean journal of applied entomology
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    • v.24 no.2 s.63
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    • pp.85-95
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    • 1985
  • It has been tried to find effective biological control measures involved in nature of soil suppressiveness to fusarium wilt of cncumber caused by Fusarium oxysporum f. sp. cucumerinum Owen. Total 28 soil samples were obtained from Jinju, Haman, Namji, Milyang and Suncheon vinyl house area. The disease response of test soil was quantified in terms of DI50 value which caculated from log-probit transformation of diseases response curves. Soils designated 5 from Jinju, 7 from Suncheon, 22 from Namji were recognized as suppressive to fusarium wilt of cucumber. This suppressiveness was completely nullified after autoclave. The disease suppressiveness of tested soil did not indicate any consistency according to either chemical property or texture of soil. Conidial germination, induction and germination of chlamydospore were markedly inhibited in supprerssive soil compared to those in intermediate or conducive soils, however, mycelial lysis did not appear to have direct relationship with disease suppressiveness of given soil. Population density of fluorescent Pseudomonads and Bacillus spp. in the soil originated from different degree of suppressiveness were not different significantly but the number of lytic bacterial plaques measured by triple layer agar method was remarkably higher in suppressive soil than that in intermediate or conducive soil.

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Occurrence of Grapholita dimorpha in Korean Pear Orchards and Cross-trapping of Its Sibling Species, Grapholita molesta, to a Pheromone Lure (국내 배과원에 복숭아순나방붙이의 발생과 유사종 복숭아순나방의 페로몬 트랩 교차 유인)

  • Jung, Chung Ryul;Ahn, Jeong Joon;Eom, Hoon Sik;Seo, Jung Heun;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.479-484
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    • 2012
  • The occurrence of plum fruit moth, Grapholita dimorpha, has been recently reported in apple orchards in Korea. It has been suspected that G. dimorpha and its related species, G. molesta, may occur simultaneously in other host plants. This study reports the occurrence of G. dimorpha in pear orchards of different localities in Korea. The identification of G. dimorpha was determined by morphological characters and the DNA marker. The cross-trapping of both species may be possible because the major sex pheromone (SP) compositions for the two species are similar. From the monitoring data, G. dimorpha and G. molesta were caught in SP lure traps of G. dimorpha and both species were also caught in SP lure traps of G. molesta. This cross-trapping of G. molesta to a SP lure of G. dimorpha varied significantly among pear orchards in different geographical localities. Furthermore, the occurrence peaks of the two species were not coincidental in all monitored orchards. These suggest that monitoring data obtained from each SP trap of both species in pear orchards may be mixed with two species, which would result in the over-estimation of population density and peak frequency on both species in pear orchards.

Change in Hemocyte Populations of the Beet Armyworm, Spodoptera exigua, in Response to Bacterial Infection and Eicosanoid Mediation (세균 감염에 따른 파밤나방 혈구 밀도 변화와 아이코사노이드 중개 역할)

  • Park, Jiyeong;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.349-356
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    • 2012
  • Eicosanoid mediates various cellular immune responses in insects. This study aimed to discover its novel action on the modulation of hemocyte populations in response to an immune challenge. Upon bacterial challenge, the last instar larvae of the beet armyworm, Spodoptera exigua, increased their total hemocyte density in 2 h, and then decreased it to a basal hemocyte density level. This rapid increase in total hemocyte density was explained by an increase of plasmatocyte and spherulocyte densities. When larvae were treated with dexamethasone (a specific phospholipase $A_2$ ($PLA_2$) inhibitor), they did not show any increase in hemocyte density in response to bacterial challenge. However, the addition of arachidonic acid (a catalytic product of $PLA_2$) to larvae treated with dexamethasone recovered the up-regulation of hemocyte density in response to bacterial infection. Among eicosanoid, cyclooxygenase (COX), but not lipoxygenase (LOX), products seemed to mediate the increase of hemocyte density in response to bacterial infection because naproxene (a COX inhibitor) inhibited the hemocyte density increase, though esculetin (a LOX inhibitor) did not. Prostaglandin $E_2$, a COX product, significantly increased the hemocyte density even without bacterial infection. These results suggest that eicosaniod mediates a rapid increase in total hemocyte density in response to immune challenge.

Accuracy and radiation exposure from image-guidance in Tomotherapy Hi-Art System (토모테라피 Hi-Art System의 영상유도 정확성 평가와 환자 피폭에 관한 연구)

  • Jang, Jae-Uk;Lim, Hyun-Soo;Han, Man-Seok;Kim, Yong-Kyun;Jeon, Min-Cheol
    • Journal of Digital Convergence
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    • v.11 no.10
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    • pp.577-584
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    • 2013
  • IGRT(Image Guided Radiation Therapy) in radiation therapy is a very useful technique in order to increase setup of patient and position reproducibility. Tomotherapy can increase accuracy of setup to take IGRT by MVCT, but it be for verified accuracy of Image guided, and MVCT occurs the exposure of patient. Through this study, IGRT accuracy of Tomotherapy is very accurate within 1.0mm. When MVCT using Tomotherapy phantom for QA, QC be taken, exposure dose is Fine(2mm Slice thickness) 3cGy, Normal(4mm Slice thickness) 1.5cGy, Corse(6mmSlice thickness) 1.0cGy. Measurement value of spatial resolution using AAPM CT performance phantom did't cause a big difference. As a result, ability of IGRT in Tomotherapy is very accurate. While obtaining image for IGRT, we should minimize expose range because patient's be exposed to radiation. We should make an effort to do accurate radiation therapy to minimize exposure of patient by selecting the appropriate thickness of MVCT depending on patient's body and treat area.

Control effect of the newly developed insecticidal protectant on Sycamore lace bug, Corythucha ciliata (Hemiptera : Tingidae) (버즘나무 방패벌레에 대한 신규 합성 물질의 방제효과)

  • Park, Hyean-Cheol;Kim, Keun-Ki;Kim, Yong-Gyun;Lee, Sang-Mong;Son, Hong-Joo;Choi, In-Soo;Shin, Taek-Soon
    • Journal of Life Science
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    • v.17 no.6 s.86
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    • pp.828-830
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    • 2007
  • To determine the effects of newly developed insecticide (Ultra) on Sycamore lace bug (Corythucha ciliata), ten leaf discs were sprayed with the Ultra solution including the other selected commercialized insecticide solutions such as Carbaryl and Pirimicarb, and also with a water-sprayed control. According to the results, the control effect of Ultra on the adults and immatures was respectively 98.9% and 97.5% after 48hr, and the corresponding values were higher than that of the other tested insecticides (i.e Carbaryl showed 84.6% for adults and 85.6% for immatures; Pirimicarb showed 83.0% for adults and 90.7% for immatures). For the Susceptibility test, LC$_{50}$ value of Ultra showed the lowest as 1.5ppm, the value of Carbaryl and Pirmicarb were 3.7 ppm and 30.4 ppm, respectively. Therefore, the insecticidal effects were better for the newly developed insecticide than the commercialized insecticides.

Pyriproxyfen Inhibits Hemocytic Phagocytosis of the Beet Armyworm, Spodoptera exigua (파밤나방(Spodoptera exigua)의 혈구세포 식균반응에 대한 피리프록시펜의 억제효과 Nalini Madanagopal)

  • Madanagopal, Nalini;Lee, Yong-Joon;Kim, Yong-Gyun
    • The Korean Journal of Pesticide Science
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    • v.11 no.3
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    • pp.164-170
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    • 2007
  • The concept of innate immunity in insects which refers to the first line of host defense constitutes the humoral and cellular components which are involved in recognition and actively participate in the elimination of the intruding foreign micro- or macro-organisms. Several recent studies suggest that juvenile hormone (JH) modulates the cellular immune reactions in response to pathogen. In this study, pyriproxyfen (a JH agonist as an insect growth regulator) was tested in its any inhibitory effect on the immune reactions of the beet armyworm, Spodoptera exigua. To this end, five different hemocyte morphotypes of final instar S. exigua were identified by phase contrast microscopy. Plasmatocytes and granular cells, which constitute about 90% of the total hemocyte count, were prominently distinguished based on their basophilic/acidophilic nature using Giemsa stain. The role of pyriproxyfen on the functional ability of hemocytes was analyzed using FITC-labeled Providencia vermicola for the phagocytic potential of the hemocytes. Both granular cells and plasmatocytes exhibited phagocytosis behavior. Pyriproxyfen significantly inhibited the phagocytosis of both cell types, proposing its novel action as an immunosuppressant.

The Tangential Projection Method for Checking Existence and Nonexistence of Radius Groove Penetration of Screw after Distal Radius Fracture Operation Used the T-type Plate (T형 금속판을 이용한 요골 원위부 골절 수술 후 나사못의 요골구 관통 유무를 확인하기 위한 접선방향 촬영법)

  • Seo, Sun-Youl;Hong, Ki-Jang;Han, Man-Seok;Kim, Yong-Kyun
    • Journal of radiological science and technology
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    • v.33 no.4
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    • pp.335-340
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    • 2010
  • This paper is about a projection method to check existence of radius groove penetration of screw after distal radius fracture operation using the T-type plate. Angle of Radius groove was analyzed by fifty one CT images that contains patients' wrist and twenty cases of radius specimens. After making radius phantom by plaster, we set the screw so that it penetrated 2.4 mm depth of radius groove. Then, we projected the phantom by X-ray in change of the elevation and supination angle of distal radius by 5 degree interval on 0~30 degree. The average value of groove angle in the wrist CT images was 14.4 degree and the radius specimens was 16.3 degree. Screws penetrating radius groove of the phantom have different lengths according to elevation angle and supination angle. Consequently, in order to confirm existence and nonexistence of radius groove penetration of the screw in tangential projection after distal radius fracture operation using the T-type plate, we recommend 5 degree of elevation angle and 20 degree of supination angle.

Liver Size Measurement Method by Ultrasonography and Reference Range based on Normal Adult Physique Index (초음파검사에 의한 간 크기 측정방법 및 정상 성인의 체격지수별 참조범위)

  • Kim, Yong-Kyun;Han, Dong-Kyoon
    • Journal of radiological science and technology
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    • v.41 no.1
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    • pp.13-24
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    • 2018
  • Liver size is an important component in the diagnosis and follow-up of diffuse liver disease when testing for liver disease using ultrasonography. However, difficulties lies in determining the presence of hepatomegaly and liver atrophy because the method used for measuring liver size differs from one examiner to another and there is no relevant standard based on body build. The present study aims to propose a more objective method for liver size measurement and a reference range based on body build. A total of 260 normal adults (130 males, 130 females) participated in the study. Ultrasonography was performed in all participants to measure the size of the right lobe, left lobe, quadrate lobe, and caudate lobe of liver. Based on Physique Index(PI), a value derived from multiplying weight(kg) by height($m^2$), size of physique was divided into three groups including Group I with PI<150, Group II with $150{\leq}PI{\leq}250$, and Group III with PI>250. Thus, mean liver size by PI and a reference range with 95% reliability were suggested. The superoinferior diameter of right lobe was $12.34{\pm}1.18cm$ in males and $11.07{\pm}0.93cm$ in females, and its reference range was 10.64~11.0cm for Group I, 11.78~12.12cm for Group II, and 13.02~13.84cm for Group III. The anteroposterior diameter(T) of left lobe was $5.93{\pm}1.09cm$ in males and $5.18{\pm}0.99cm$ in females, and its reference range was 4.77~5.17cm for Group I, 5.49~5.79cm for Group II, and 6.68~7.44cm for Group III. The transverse diameter was $3.51{\pm}0.60cm$ in male participants and $3.42{\pm}0.49cm$ in female participants and its reference range was 3.29~3.51cm for Group I, 3.36~3.55cm for Group II, and 3.52~4.0cm for Group III. The caudate lobe index was $11.65{\pm}2.88cm^2$ in males and $9.62{\pm}2.18cm^2$ in females and its reference range was $8.83{\sim}9.75cm^2$ for Group I, $10.62{\sim}11.47cm^2$ for Group II, and $11.89{\sim}14.26cm^2$ for Group III. As a basic measurement method of liver size, the present study suggests measuring the superoinferior diameter for right liver lobe, the anteroposterior diameter for left liver lobe, the transverse diameter for quadrate lobe, and the caudate lobe index for caudate lobe. It is expected that this method along with its relevant reference range can be used as useful indicators in determining hepatomegaly and liver atrophy upon the diagnosis and follow-up testing of diffuse liver disease.

Characteristics and Distribution of Microorganisms in a Rice Straw Compost for Cultivation of Button Mushrooms (Agaricus bisporus) (양송이 재배에서 볏짚 배지의 발효 단계별 관여 미생물의 분포양상 및 특성)

  • Lee, Chan-Jung;Yoo, Young-Mi;Moon, Ji-Won;Cheong, Jong-Chun;Kong, Won-Sik;Kim, Yong-Gyun;Lee, Byung-Eui;Yoon, Min-Ho;Sa, Tong-min
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.43-53
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    • 2017
  • In this study, we analyzed the densities and taxonomic characteristics of various microorganisms that play important roles in Agaricus bisporus culture medium composting, and examined changes in the levels of decomposition-related enzymes secreted by these microorganisms. Various microorganisms such as thermophilic bacteria, actinomycetes, fluorescent Pseudomonas spp., and filamentous bacteria are closely associated with culture medium composts of Agaricus bisporus. The population densities of microorganisms change, and harmful bacteria disappear during thermophilic composting. Psychrobacter sp., Pseudomonas sp., Bacillus sp., and Pseudoxanthomonas sp. accounted for the highest proportion of bacteria in the culture media during outdoor composting, whereas Bacillus sp. and Psychrobacillus sp. were dominant after pasteurization. Cellulose and hemicellulose enzymes of the microorganisms were important at an early stage of rice straw composting and after decomposition of carbon sources, respectively. Microorganisms that secreted these enzymes were present in the second and third turning stage of composting.

Studies on the Activity and Stability of Egg White lysozyme (난백 lysozyme의 역가측정과 안정성에 관한 연구)

  • 이성기;유익종;김기성;김영붕
    • Korean Journal of Poultry Science
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    • v.17 no.2
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    • pp.109-114
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    • 1990
  • Enzymatic activity of isolated Lysozyme from egg white by cation ion-exchange chromatography was detected with various methods and stability of lysozyme in solution was studied by heat and pH treatments. Lysozyme activity refered to mg pure lysozyme/mg sample was more accurate although it needed standard lysozyme. But lysozyme activity refered to units/mg sample could be detected easily and reducted total detection time. Enzymatic activity of isolated lysozyme which dissolved in 0.066M phosphate buffer(pH 6.3) and then incubated at $37^{\circ}C$ for 2hr was increased remarkably on the lysis of Micrococcus lysodeikticus. The activity of isolated lysozyme by CM Sephadex C-25 was higher in eluting solution of above O. D. 1.0 at 640nm and attained 36, 000 units/mg solid. The stability of isolated lysozyme was decreased by various heat treatment. Activity began to decrease above 6$0^{\circ}C$ and dropped rapidly at $100^{\circ}C$. Especially, 35% loss of activity occured in 0.066M phosphate buffer at $100^{\circ}C$. for 15min. The stability of lysozyme was also affected by pH. lysozyme was very stable in acidic solution but in alkaline solution. Enzymatic activity showed maximum value at pH 3.0 solution while decreased rapidly above pH 6.0 solution.

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