• Journal of the Speleological Society of Korea
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• no.89
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• pp.1-15
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• 2008

In this study, the inhabitant were investigated at the six sites in the cave which is located in Giwha-ri, Mitan-myen, Pyeong Chang-gun from January to June in 2005. As the result of observation, collection, researching, It was confirmed as all animals species were classified by 19 species, 4 classes, 11 orders, 16 families. Among the investigated animals, Diestrammena asynamora Adelung distributed 66.6% and Antrokoreana gracilipes Verhoeff distributed 22.4%. They were confirmed as a dominant species. Among the observed animals, Troglobite with ecological characteristics was 5.3%, troglophiles were 21.1% and trogloxenes were 73.7%. Among them, There were the largest number of species in trogloxenes. In the class manmalia, Rhinolphus ferrumequinum korai Kuroda and two Myolis daubentoni ussuriensis Ognev's species were observed. Among them Rhinolphus ferrumequinum korai Kuroda distributed 58.9%, so they were confirmed as a dominant species. The largest number of Triphosa dubitata Linnaeus, trogloxenes, were observed in January and February in winter. 503 individual of Antrokoreana gracilipes Verhoeffs, troglobites, were observed only in June. Diestrammena asynamora Adelung, troglophiles, appeared the large number of species in sping, especially, in April and May.

• Journal of the Speleological Society of Korea
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• v.5 no.6
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• pp.20-22
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• 1980

동굴 속이라는 특수환경 속에 사는 동물을 우리는 동물생물(Gavernicole)이라 부르며, 이 동굴생물은 i)외래성(trogloxene) : 개구리(Amphibia) 따위의 양서류와 파리(Piptera) 등. ii) 호동굴성(troglophile) : 푸라나니아(Tricladia), 거미류(Araneido) 박쥐(Chirptera) 등. iii) 진동굴성(troglobiont): 새우류(Amphipoda), 톡톡이(Collembala), 좀류(Thysanura), 노래기(Aiplopada) 등. 3가지로 분류하고 있다.(중략)

• Journal of the Speleological Society of Korea
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• no.88
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• pp.7-25
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• 2008

In order to accumulate the basic informations to assess the scientific and natural values of the Hoe-ok Cave located in Mountain Chi-ak, Seong-nam 2 ri, sin-rim myeon, the distribution of the species in the cave were investigated from November 2004 to June 2005. The results are as follows. The species identified in the Hoe-ok Cave were 34 species, 28 family, 18 order, 8 classes. The dominant species were the 14 species(41.2%), 11 family, 7 order in the Class of Insecta. The ecological distribution of the Heo-ok Cave, 8 species(23.5％) of troglobite, 7 species of troglophiles(20.6％), 19 species of trogloxenes(55.9％) were found and the share of the species indigenous to the cave among the population was 44％, and the species which came out of the cave occupied the larger portion among the populations inside the whole cave.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2005.11a
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• pp.325-326
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• 2005

해양에 적조가 발생하면 해수의 산소결핍 현상을 유발하고 동식물의 성장과 생존에 필수적인 태양광을 차단하여 해양 동물뿐 아니라 식물에게까지 치명적 피해를 준다. 또한, 대형 선박의 안정수(ballast water) 유입시 안정수 내에 포함되어 그들의 생활공간을 이동하면서 선박으로부터 다시 유출될 때 신생활공간에 외래 생물체로 침입하여 그곳의 기존 생태계를 파괴시키거나 교란하는 외래침입생물체는 해양생태계 환경파괴의 주범이다. 따라서 이들 두 종류의 해양생물은 부분적으로 상관성을 지니는 해양수산업계의 천적이며, 해양생태환경 파괴의 원인 제공자로써 마땅히 과학적 방법으로 제거되거나 방지되어야 한다. 이 논문은 해양수산업 계에 막대한 피해를 끼치며 해양생태환경 파괴의 주범으로 등장한 적조생물과 외래침입생물체들이 해수로부터 제조한 플라즈마 반응생성물에 처리된 결과를 분석하여 그 응용성을 조사하였다.

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.31-31
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• 2002

건강보조식품의 홍수와 함께 우유보다 소화흡수가 좋고 건강식을 즐겨하는 현대인들의 취향에 발맞추어 상대적으로 유량과 산육성이 좋은 외래산양의 유입이 급속하게 늘어남으로써, 일부 재래산양 사양 농가들은 생산성 향상을 목적으로 재래산양과 외래산양의 무분별한 교배를 시키므로써 재래산양의 유전자원의 보존문제가 대두됨과 동시에 재래산양의 개량의가속화, 근친교배에 따른 문제점, 계절번식에 따른 산자생산의 한계 등은 재래산양의 인공수정에 대한 깊이 있는 연구로 이어져야 하겠기에 본 연구에서는 38 두의 재래산양을 공시하여 실험한 결과 여름과 겨울에 채정한 정액보다는 봄가을에 채정한 정액의 량이 2.0㎖로 여름과 겨울보다 많았으며, 정자농도는 봄에 21.0×10/sup 8//㎖로 가장 짙었고, 총정자수는 봄에 42.0×10/sup 8/로 가장 많았으며, 운동성은 가을에 90(motility)로 가장 좋았다. (중략)

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 1997.10a
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• pp.5-12
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• 1997

외래 유전자를 이식하여 형질전환동물을 생산하는 방법은 현재 약 3가지 정도가 실제 이용되고 있다. 현재 가장 많이 사용되고 있는 방법으로는 DNA 미세주입법인데 생쥐와 비교하여 다른 동물에서의 형질전환효율은 상당히 낮은 것으로 나타나 형질전환가축의 생산을 위해서는 많은 비용과 시설 및 노동이 필요하여 실용성에 문제를 갖고 있다. 각 가축에 적합한 DNA 미세주입 조건을 확립시키고 형질전환동물의 생산효율을 높이는 연구가 필요하다. 가축에서 형질전환기술이 실제로 응용되고 있는 분야로는 대량생산이 어려운 의약품을 형질전환가축의 젖으로 합성 분비시키게 함으로써 생리적으로 활성이 있는 의약품의 대량생산이 가능할 것으로 예상된다. Growth Hormone이나 Growth Factor들을 이용한 성장과 관련된 형질전환가축의 기술은 예상했던 것보다 큰 성과는 없었는데 이식 유전자의 과잉발현으로 인한 부작용으로 실용화될 수 없었다. 그러므로 형질전환동물의 실용화를 위해서는 효율적인 유전자 이식방법의 개발과 이식 유전자의 발현으로 인한 부작용을 최소화하면서 좋은 표현형을 얻을 수 있도록 이식유전자의 발현을 인위적으로 조절할 수 있는 regulatory system의 개발과 가축의 경제형질에 관여하는 유전자의 식별이 필요하다.

• Korean Journal of Environment and Ecology
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• v.34 no.2
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• pp.106-114
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• 2020

The development of transportation and the expansion of the pet market has become the main causes of the increase in the cross-border migration of non-native species. Moreover, recent sales over the Internet were a factor that has promoted pet trades. While the import of non-native species has been steadily increasing in the Republic of Korea, it is not clear how these imported species are traded and how large the trade is. Considering that most of the non-native species found in the wild are the results of release by humans understanding the present situation of pet trades can identify potential non-native species that can enter the wild. This study surveyed the number of species, frequency, and prices of non-native amphibians and reptiles sold in 25 online pet shops from January 22 to February 10, 2019. The results of the survey showed that a total of 677 species of non-native amphibians and reptiles were sold, and the Squamata group accounted for the largest part of them at 65.4% (443 species). The number of non-native amphibians and reptiles sold in online pet shops in 2019 was 2.1 times larger than the 325 species officially imported in 2015. The non-native amphibians and reptiles sold in most pet shops were Litoria caerulea (21 shops) and Correlophus ciliatus (24 shops). The lowest price for non-native amphibians and reptiles was 3,000 won, and the highest price was 100 million won for Rhacodactylus leachianus of Squamata. Among the non-native amphibians and reptiles sold in online pet shops, 11 species were found in the wild and were sold at relatively low prices. We confirmed that Mauremys reevesii, an endangered species class II and natural monument no. 453, and American bullfrogs (albino), an ecosystem disturbing species, were being sold in online pet shops. Moreover, 21.6% of the 677 non-native amphibians and reptiles sold in online pet shops were species designated as CITES. The results of this study can be the important reference data for understanding the status of non-native amphibians and reptiles that are imported and sold in Korea and evaluating and predicting the potential for them to enter the wild.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.239-239
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• 2004

본 연구에서는 효과적인 유전자를 발현시킬 수 있는 retrovirus vector system을 구축하기 위하여 네 가지 promoter를 비교하였다. 일반적으로 사용되고 있는 RSV (Rous sarcoma virus), UbC (Ubiquitin), β-actin, CMV(cytomegalovirus) promoter 하에 GFP (green fluorescent protein)를 표지유전자로 사용하였다. 또한 WPRE (Woodchuck hepatitis virus Posttranscriptional Regulatory Element) 서열을 도입하여 각각의 promoter 하에서 GFP 유전자의 발현 증가 여부를 검정하였다. (중략)

• Korean Journal of Animal Reproduction
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• v.23 no.1
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• pp.13-18
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• 1999

The totipotential spermatogonial stem cells of adult testis which give rise to mature sperm cells is well-known to incorporate foreign DNA as well as those of somatic cells. Also, the integration rates of foreign DNA after haploid stages are generally known to decrease and /or is simply bound foreign DNA into the sperm plasma membrane. To overcome these problems, liposome and DNA complexes were used to determine how direct injection of these complexes into testis were integrated into sperm genome and resulted in transgenic offspring. To study this purpose, cation liposome was gently mixed with WAF/hGH DNA (1 : 2) and the complexes were injected into testis. At 10, 20, 40, 60, and 80 days after direct injection into testis, mature sperm cells were recovered by using artificial virgin method from two goats and each semen except a part of semen used for DNA analysis such as PCR or Southern blotting was cryopreserved for the artificial insemination. The results obtained in this study are summarized as follows. 1. By PCR, the presence of exogenous DNA was confirmed up to 80 days after injection with liposome/DNA complexes. The highest integration rates were obtained at day 40 after direct injection. This results suggested that spermatogonial stem cells were integrated exogenous DNA into their genome. 2. Among 23 Korean Native Goats which were artificially inseminated, 4 goats resulted in pregnancy and produced 7 young goats. 3. Two young goats were confirmed as a transgenic by PCR and Southern blot analysis. Therefore, our results suggested that testis-mediated gene transfer can be used as a feasible tools for the production of transgenic livestock.

• Korean Journal of Ecology and Environment
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• v.47 no.4
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• pp.239-246
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• 2014

To cope with the potential risks associated with invasive alien species, the Korean Government implemented the Biodiversity Act in 2014. Among the alien species not yet introduced into that country, 24 have been designated as alert species. These include mammals, birds, fish, mollusks, insects, and plants that may invade and cause serious harm to the ecosystem. Approval from the Ministry of Environment is required to import or carry any of them. Although these measures are more advanced than those from the previous legal framework, several terms still need to be improved. First, the category of alert species should cover not only those not yet introduced but also those that are being raised or cultivated at aquariums, botanical gardens, and zoos. Second, for applicants who intend to import or carry alert species, the government must provide them with detailed standards for the ecological risk assessment of alert species as well as guidelines for their safe use in Korea to prevent their unregulated release from confinement facilities into natural environments. Third, tools and protocols should be developed for early detection and rapid responses to those escapes.