• Title/Summary/Keyword: 옥수수 선택성

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Identification of Agrobacterium tumefaciens from Soil and Transformation of Maize (토양으로부터의 Agrobacterium tumefaciens의 분리, 동정 및 옥수수의 형질전환에 이용)

  • 노광수;강봉중
    • KSBB Journal
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    • v.7 no.3
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    • pp.191-200
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    • 1992
  • Several strains of Agrobacterium tumefaciens were isolated from soil in the Taegu area and characterized to develop some useful vector systems for higher plant genetic engineering. The selected colonies had a unique form, and strains from the colonies were capable of tumor formation on the sunflower leaf surface. They had a large plasmid. The restriction analysis showed that they were another kinds of Ti plasmic compared with C58 and Ach5. The isolated strains were identified as the nopaline type and also as biovar 1 A. tumefaciens, according to their tumor morphology, blophyslcal and biochemical characteristics. One of the isolated strains, AK204 was transformed with binary vector (pGA642), having selectable marker (Kmr, Tcr). Furthermore, maize tissue cells were transformed by cocultivation with AK204/pGA642, and the transformants were selected on the selective medium and identified using PAGE patterns of their soluble proteins.

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Influence of 3-(N-methyl-N-X(Sub.)Phenylaminooxoacetyl) Group on the Herbicidal Activity of Imazapyr Derivatives (Imazapyr 유도체의 제초활성에 미치는 3-(N-methyl-N(X)-치환-Phenylaminooxoacetyl) Group의 영향)

  • Sung, N.D.;Ryu, T.S.;Chang, H.S.;Kim, D.W.
    • Applied Biological Chemistry
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    • v.37 no.6
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    • pp.516-521
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    • 1994
  • New seventeen imazapyr derivatives, 2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)-3-(N-methyl-N-X(sub.)-phenylaminooxoacetyl)pyridine, 6 were synthesized and their pre-emergence herbicidal activity$(pI_{50})$ in vivo against Corn (Zea mays L.) and Pigweed (Amaranthus viridis L.) were studied by the pot test under paddly conditions. Quantitative structure activity relationships (QSARs) were analyzed using the physicochemical parameters of substituent(X) on the phenyl ring of 3-(N-methyl-N-X(sub.)-phenylaminooxoacetyl) group and regression analysis. The herbicidal activities were related to the steric effect of X-substituent. The effect was rationalized by paraholic function of MR and $L_1$, where the optimal values were MR=5.56 (Zea mays L.) and $L_1=3.34\;{{\AA}}$ (Amaranthus viridis L.). Among them, 2,5-difluoro substituted compound, 6i showed good herbicidal activity against Pigweed with excellent tolerance to Corn.

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Synthesis and Characterization of HEMA-PCL Macromer Grafted onto Starch (옥수수전분에 HEMA-PCL Macromer를 그래프팅시킨 공중합체의 합성 및 특성)

  • 공원석;진인주;김말남;김수현;윤진산
    • Polymer(Korea)
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    • v.24 no.2
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    • pp.141-148
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    • 2000
  • Polycaprolactone (PCL) was blended with corn starch to produce biodegradable compost films and the biodegradability and mechanical properties were investigated. As the compatibilizer for the immiscible PCL/starch blend, 2-hydroxyethylmethacrylate (HEMA)-PCL macromer was grafted onto starch by initially grafting HEMA to starch and then grafting of PCL onto HEMA via ring opening polymerization of $\varepsilon$-caprolactone. When biodegradability of the PCL grafted starch-g-DEMA copolymers was compared with that of starch by the modified Sturm test, graft copolymers degraded at much slower rates due to the presence of the non-degradable HEMA. With the addition of the graft copolymer up to 5 wt% to the blend, the elongation-at-break of the starch/PCL blend increased substantially, while the tensile strength and modulus did not change much. SEM observation of the blend containing 2 wt% copolymer clearly indicated that the interfacial adhesion between the starch and PCL was strengthened by the copolymer.

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Comparison of Forage Productivity and Outbreak Rate Between Corn Varieties in Rice Black-Streaked Dwarf Virus (RBSDV) Prevalent Area (검은줄오갈병 발생이 심한 지역에서 옥수수 품종의 발병률과 사초 생산성 비교)

  • Choi, Gi Jun;Lim, Young Chul;Yoon, Sei Hyung;Ji, Hee Chung;Lee, Sang-Hoon;Jung, Min Woong;Seo, Sung;Park, Hyung Soo;Kim, Ki-Yong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.33 no.2
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    • pp.111-116
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    • 2013
  • This experiment was carried out to select a resistant corn variety for rice black-streaked dwarf virus (RBSDV) disease in a RBSDV prevalent area (Gochang of Jeollabukdo) from 2006 to 2008. Ten corn varieties for silage preparation were cultivated with first cropping and second cropping system in an RBSDV field, and were tested outbreak rates of RBSDV and dry matter (DM) yield of forage. The outbreak rates of RBSDV were significantly different between corn varieties. Therefore, the resistance degree of corn varieties for RBSDV were divided into 4 groups; very resistant ('Kwangpeyongok' and 'Kwanganok'), resistant ('P3156' and 'P3394'), mildly resistant ('Cheonganok' and 'P32P75') and susceptible ('Suwon19', 'DK697' and 'GW6959') groups respectively. DM yield of forage in RBSDV fields exhibited comparatively significant differences between varieties (p<0.05). DM yield of resistant varieties ('Kwangpeyongok', 'Kwanganok', 'P3156' and 'P3394'), were higher (14~26%) than those of susceptible varieties ('Suwon 19', 'DK697' and 'GW6959'). Therefore, resistant varieties were recommended for increasing forage productivity in field of RBSDV prevalent areas.

Growth Characteristic and Productivity of Forage Corn Varieties Sown at the Last Ten Days of May in Central Region of Korea (중부지역에서 5월 하순 파종한 사료용 옥수수의 품종별 생육특성과 조사료 생산성)

  • Choi, Gi Jun;Jung, Jeong Sung;Choi, Ki Choon;Hwang, Tae Young;Kim, Ji Hye;Kim, Won Ho;Lee, Eun Ja;Sung, Kyung Il;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.1
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    • pp.17-23
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    • 2019
  • This experiment was carried out to evaluate the characteristic and productivity of forage corn varieties sown at the last ten days of May at Cheonan of Korea on 2017 and 2018. Forage corn varieties sown on 24th May 2017 and $25^{th}$ May 2018 were emerged in 5th and 1st June, respectively. Silking date of forage corns was different among varieties(p<0.05). Stem length of forage corns was not different among the varieties, except Kwangpyeongok(KPA) and Nero IT. Stem diameter of forage corns was not different amaong the varieties. Outbreak rate of black streaked dwarf virus was lower than 3% in all varieties. Total digestible nutrient(TDN) yield was different among corn varieties(p<0.05), which was higher in P32P75, followed by P2088, P31N27, and KPA. The TDN yield of varieties had direct correlation with stem length, stem diameter and ear yield, respectively(p<0.01). Average of TDN in all varieties was 69.8%. These results suggested that forage corn variety with late-seeding adaptability is more important for increasing the productivity of forage corn If harvesting of winter forage crops have to be finished in the middle ten days of May in middle region of Korea.

Effect of Rice Black-Streaked Dwarf Virus(RBSDV) Infection Rate on Forage Productivity of Corn Variety in Middle District of Korea (중부지역에서 검은줄오갈병 발생이 사료용 옥수수 품종의 조사료 생산성에 미치는 영향)

  • Choi, Gi Jun;Lim, Young Chul;Yoon, Sei Hyung;Ji, Hee Chung;Lee, Sang-Hoon;Jung, Min Woong;Kim, Won;Park, Hyung Soo;Kim, Ki-Yong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.1
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    • pp.44-49
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    • 2017
  • This experiment was carried out to study the effect of rice black-streaked dwarf virus(RBSDV) infection rate on forage productivity of corn varieties in Cheonan of chungcheongnamdo from 2006 to 2008. Forage corn varieties of 10 were cultivated with first cropping(seeding in the last ten days of April) and second cropping(seeding in the last ten days of May) system in field and tested the infection rates of RBSDV and productivity of forage. The Infection rate of RBSDV was significant difference between corn varieties in middle district of Korea. Resistant corn varieties for RBSDV were 'Kwanganok', 'P3156', 'Kwangpeyongok' and 'P3394' but susceptible varieties were 'Suwon19', 'DK697', 'GW6959' and NC+7117. Dry matter(DM) yield of forage corn according with infection rates of RBSDV in field was significant difference between varieties(p<0.05). DM yield of susceptible varieties, 'Suwon 19', 'DK697' and 'GW6959' was lower about 20% than that of resistant varieties, 'Kwangpeyongok' and 'P3156'. For increasing the productivity of forage corn, recommend of resistant varieties for RBSDV and control of seeding time are very important in middle district of Korea.

농산 폐기물을 이용한 xylose, arabinose, cellulose 생산공정

  • Sin, Hyeon-Seung;Yu, Yeon-U
    • 한국신재생에너지학회:학술대회논문집
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    • 2005.11a
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    • pp.286-298
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    • 2005
  • 농산 폐자원으로부터 식품 및 의약품 소재로 쓰이는 xylose, arabinose, cellulose를 생산하였다. 농산 폐자원은 우리나라 실정에 적합한 볏짚과 옥수수 껍질을 선택하였으며, 공정수율은 xylose와 arabinose는 약 15$\sim$20%(w/w), cellulose 는 20%(w/w)로 나타났다. 폐자원을 활용하는 개발된 공정중에는 미생물 유전자 재조합 기술을 응용하여 고역가의 효소생산 system을 개발하여, 생산된 효소를 가수분해 공정 과정에 투입하여 경제성이 높고 친환경적인 기술로 확립하였다. 재조합 미생물과 xylose, arabinose 정제공정은 신뢰 높은 재연성을 나타냈으며 xylose 제조법과 xylitol 발효법은 package 형태로 기술 이전을 준비하고 있다.

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The Effects of TEMPO, NaBr and Temperature on the Selective Oxidation of Primary Alcohol Groups in Corn Starch (TEMPO와 NaBr 의 농도 및 온도가 옥수수 전분의 선택적 산화반응에 미치는 영향)

  • Suh, Dong-Soon;Chang, Pahn-Shick;Kim, Kwang-Ok
    • Korean Journal of Food Science and Technology
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    • v.33 no.1
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    • pp.40-44
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    • 2001
  • The effects of TEMPO and NaBr concentration, and temperature on the selective oxidation of primary alcohol groups in corn starch were examined. Reaction time decreased with the increased levels of TEMPO, NaBr and temperature up to 1.7 mM and 100 mM per 100 mM anhydroglucose unit(AGU) and $14^{\circ}C$, respectively, and did not change appreciably at the higher levels. Yield decreased with the increased NaBr levels and was not affected TEMPO level and the temperature. NaBr level and temperature showed negative effects on the selectivity. But the selectivity was not affected by NaBr level and temperature until 100 mM/100 mM AGU and $6^{\circ}C$. TEMPO had no effect on the selectivity significantly.

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Selective Mechanism of Oxvfluorfen and Chlormethoxynil in Crops and Weed Species (Oxyfluorfen과 Chlormethoxynil의 선택작용성(選擇作用性))

  • Lee, J.H.;Pyon, J.Y.;Lee, J.Y.
    • Korean Journal of Weed Science
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    • v.14 no.4
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    • pp.280-290
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    • 1994
  • The germination and the nutrient culture tests in the growth chamber and pot experiment under two types of soil conditions were conducted to determine the selectivity of oxyfluorfen and chlormethoxynil in crops and weeds and for characterization of selective mode of action, the absorption study was also conducted with different absorption methods and application time using $^{14}C$-oxyfluorfen. Oxyfluorfen showed more growth inhibitory effects than chlormethoxynil. In the nutrient culture test, rice growth was greatly inhibited at 2-leaf stage than at 4-leaf stage, and the shoot parts were more inhibited than the root parts. By preemergence application of both herbicides, higher growth inhibition was observed in sandy loam soil than in clay loam soil. Absorption and translocation of $^{14}C$-oxyfluorfen were higher by foliar application than by root treatment, and selectivity of crops and weed species may be explained partly by the amount of absorption.

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Synthesis and herbicidal activities of N-[4-Cyano-2-fluoro-5-(substituted)phenyl]-3,4,5,6-tetrahydrophthalimide (N-[4-Cyano-2-fluoro-5-(substituted)phenyl]-3,4,5,6-tetrahydrophthalimide 유도체의 합성과 제초활성)

  • Ryu, Jae-Wook;Chung, Kun-Hoe;Ko, Young-Kwan;Woo, Jae-Chun;Koo, Dong-Wan;Kim, Tae-Joon;Choi, Jung-Sub;Park, Chae-Hyun;Kim, Dae-Whang
    • The Korean Journal of Pesticide Science
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    • v.9 no.1
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    • pp.108-111
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    • 2005
  • A series of N-[4-cyano-2-fluoro-5-(2-pyrimidinyloxy, 2-benzyloxy or 2-pyridinyloxy)phenyl]-3,4,5,6-tetrahydrophthalimides was synthesized, and the herbicidal activities of those deivatives were evaluated through pre- and post-emergence application under upland conditions in a greenhouse. The results showed that most compounds resulted in stronger herbicidal activity on broadleaf weeds than on grass weeds and higher through post-emergence than pre-emergence application. The N-[(4-cyano-2-fluoro-5-(2-pyrimidinyloxy) phenyl]-3,4,5,6-tetrahydrophthalimide showed the best weed control efficacy and marginal corn safety at a rate of 60 g/ha through pre-emergence application.