• Journal of Animal Science and Technology
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• v.51 no.3
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• pp.201-206
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• 2009

Seventeen porcine microsatellite (MS) markers recommended by the EID+DNA Tracing EU project, ISAG and Roslin institute were selected for the use in porcine individual and brand identification. The MSA, CERVUS, FSTAT, GENEPOP and API-CALC programs were applied for calculating heterozygosity indices. By considering the hetreozygosity value and PCR product size of each marker, we established a MS marker set composed of 13 MS markers (SW936, SW951, SW787, S00090, S0026, SW122, SW857, S0005, SW72, S0155, S0225, SW24 and SW632) and two sexing markers. The expected probability of identity among genotypes of random individuals (PI), probability of identity among genotypes from random half sibs ($PI_{half-sibs}$) and among genotypes of random individuals, probability of identity among genotypes from random sibs($PI_{sibs}$) were estimated as $2.47\times10^{-18}$, $6.39\times10^{-13}$ and $1.08\times10^{-8}$, respectively. The results indicate that the established marker set can provide a sufficient discriminating power in both individual and parentage identification for the commercial pigs produced in Korea.

• Journal of Animal Science and Technology
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• v.50 no.6
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• pp.753-762
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• 2008

The primers for RT-PCR and RACE-PCR were designed by aligning the pig genomic sequence and the human complement factor B(CFB) coding sequence(CDS) from the GenBank. Each PCR product was amplified in pig cDNA and sequencing was carried out. The CDS length of pig CFB gene was determined to be 2298 bp. In addition, the pig CDS was more longer than human and mouse orthologs because of insertion and deletion. The identities of porcine nucleotide sequences with those of human and mice were 84% and 80%, and the identities of amino acids were 79% to 77%, respectively. Three complement control protein(CCP) domains, one Von Willebrand factor A(VWFA) domain and a serine protease domain, that are revealed typically in mammals, were found in the pig CFB gene. Based on the CDSs determined, the primers were designed in intron regions for amplification of entire length of exons. In amplification and direct sequencing with genomic DNAs of six pig breeds, three cSNPs(coding single nucleotide polymorphisms) were identified and verified as missense mutations. Using the Multiplex-ARMS method, we genotyped and verified the mutations identified from direct sequencing. To demonstrate recrudescence, we performed both direct sequencing and Multiplex-ARMS with two randomly selected DNA samples. The genotype of each sample exhibited the same results using both methods. Therefore, three cSNPs were identified from pig CFB gene and that can be used for haplotype analysis of the swine leukocyte antigen(SLA) class III region. Moreover, the results indicate that the Multiplex-ARMS method should be powerful for genotyping of genes in the SLA region.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.25-34
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• 2010

Although whole crop barley (Hordeum vulgare L.) is now widely grown as a silage crop in Korea, forage production and silage quality of that for organic farm have not been published. Therefore, this experiment was conducted to evaluate the effect of harvest stage on forage production and quality of organic barley, and effect of field wilting and crushed rice for shortening of harvest date and improvement of forage quality. The experiment was split-plot design with three replications. Main plots were heading, milking and yellow stages, and sub-plot were field wilting for I day, crushed rice 10% (CR10%) and 15% (CR15%) treatments. The dry matter (DM) contents of heading, milking and yellow stages were 12.8%, 21.9% and 29.8%, respectively. The DM yields of heading, milking and yellow stages were 10,346, 15,819 and 18,336 kg/ha, respectively, and the total digestible nutrients (TDN) of these were 6,288, 9,550 and 10,178 kg/ha, respectively. The pH of milking stage showed low 4.00 pH values. The crude protein, ether extract, crude ash, non-fiber carbohydrate (NFC) and TDN contents were decreased as harvest stage progressed, while neutral detergent fiber (NDF) and acid detergent fiber (ADF) were increased. The crude ash (CA) of milking stage showed the lowest among harvest stages. Field wilting and crushed rice treatments decreased CA, NDF and ADF contents, and increased NFC and TDN contents. In vitro dry matter digestibility (IVDMD) decreased with progressed harvest stage, while field wilting and crushed rice treatments increased that of barley silage. Lactic acid and total organic acid contents of milking stage were the highest, and butyric acid of milking stage was the lowest among harvest stage. The good effect of field wilting and crushed rice was observed in heading stage. The experiment results indicate that optimum harvest stage of barley silage for organic was milking stage. The field wilting and crushed rice additive could be recommended as effective method for shortening harvest date and increasing forage quality of organic barley silage

• Journal of Animal Science and Technology
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• v.50 no.5
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• pp.667-676
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• 2008

This experiment was conducted to investigate the effects of dietary supplementation of various Lactobacilli strains on growth performance, intestinal microflora, ileal villi development and ileal mucosal surface of broiler chickens. Six hundred 1-d-old male chicks(Avian) were randomly divided into five groups of four replicates, each replicates containing 30 birds and fed corn-soybean meal diets containing Latobaillus were isolated from cecum of chichens at 107cfu/g diet for five weeks. The treatments were control(antibiotics-free diet), Lactobacillus crispatus avibro1(LCB), Lactobacillus reuteri avibro2(LRB), Lactobacillus crispatus avihen1(LCH) or Lactobacillus vaginalis avihen2(LVH). The body weight gain in groups fed Lactobacillus significantly improved as compared to those of control(P<0.05). Feed intake was not statistically different among the groups. The number of Lactobacillus in ileum and cecum of chicks fed various Lactobacillus tended to be increased or significantly increased as compared to those of the control(P<0.05), but there was difference by age of chicks and species of Lactobacillus. The number of yeast was significantly increased in cecum and ileum at three weeks old chicks fed Lactobacilli compared with the control(P<0.05). The anaerobes’ number of ileum and cecum tended to increase or significantly increased in Lactobacillus treatments compared with the control(P<0.05). The ileal villi length extended greatly at three weeks of age in groups fed Lactobacillus compared with the control(P<0.05). The length of ileal villi in chicks fed Lactobacillus was continuously increased up to five weeks of age, but did not increased in the control(P<0.05). Lactobacillus was found on ileal mucosal surface. And ileal mucosal surface was maintained better with Latobacillus feeding. These results suggest the possibility that cecal Lactobacillus of chickens could be used as probiotics by improving the growth performance and promoting development of ileal villi in broiler chicks.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.509-516
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• 2003

Growth curves were estimated for 1083 female Korean cattle raised in Daekwanryeong branch, National Livestock Research Institute (NLRI). Comparisons were made among various growth curve models for goodness of fit for the growth of the cows. Estimated growth curve functions were $W_t=370.2e^{-2.208e^{-0.00327t}$ for Gompertz model, for von Bertalanffy model, and $W_t=341.2(1+5.652e^{-0.00524t})^{-1}$ for Logistic model. Ages at inflection estimated from Gompertz model, von Bertalanffy model and Logistic model were 242.2 days, 191.5 days, and 330.5 days respectively, body weight at inflection were 136kg, 115kg, and 170kg, and daily gain at inflection were 0.445kg, 0.451kg, and 0.446kg. The predicted weights by ages from Gompertz model, von Bertalanffy model, and Logistic model were onsistently overestimated at birth weight and underestimated at 36 month weight. The von Bertalanffy model which had a variable point of inflection fit the data best.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.289-296
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• 2002

A broiler experiment was conducted to investigate the effect of supplementing yeast culture (Saccharomyces cerevisiae, Pichia pastoris) on the growth performance, small intestinal microflora and immune response in broiler chickens. One thousand hatched broiler chickens(Ross$^{(R)}$) were assigned to 6 treatments: control (basal diet), CTC; chlorotetracycline 100ppm, YC-SC; yeast culture(Saccharomyces cerevisiae) 0.3%, YC-PP; yeast culture(Pichia pastoris) 0.3%, RPPC-0.1; refined Pichia pastoris culture 0.1%, RPPC-0.3; refined Pichia pastoris culture 0.3%. There were no significant differences in growth, feed intake, feed efficiency and mortality among the treatments. However, chickens fed diets with yeast cultures showed numerically higher weight gain than those fed the control diets. Supplementation of yeast cultures and CTC improved feed efficiency and decreased mortality compared to control. Nutrient digestibilities were not affected by the dietary treatments. Total number of Lactobacilli in small intestine was higher while that of Cl. perfringens was lower with yeast culture treatments than control. Small intestine E. coli population of RPPC-0.3 treatment was significantly lower than that of the control. The serum IgG concentration tended to be higher in broilers fed yeast cultures than those fed the control and CTC diet. In conclusion, the supplementation of yeast culture products showed, although not significant but, numerical advantages in productivity and profile of microbial flora and serum IgG compared to the control and CTC supplementation.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.443-452
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• 2006

The four rice(Oryza sativa L.) lines, previously demonstrated to have vigorous herbage production at the vegetative growth stage, were investigated for their growth traits, dry matter yield and feed value using the twice-cutting system that 1st-cut is harvested at panicle formation stage and 2nd-cut at the yellow ripen stage. The twice-cutting system is regarded as an effective method in the production of forage rice by escaping lodging and insect damages caused by the overgrowth and typhoon around the heading stage.The feed value of the twice-cut rice plant of there lines were compared with the control of normal, cutting at the yellow ripen stage. The relative feed value(RFV), the total digestible nutrient(TDN) and the crude protein(CP) content of the twice-cut straw were higher the control in every lines. However, the dry matter yield (DMY), represented by the summed forage production of 1st and 2nd cut-rice plant, were lower the control, and the DMY of straws were increase but grains were decrease for twice-cutting system. In addition, TDN yield showed the similar tendency with DMY.Recently, as one of the breeding aims the researches for improving the digestibility of rice straw have been required. In this regard, twice-cutting system is recognized with the fact that it has the effect which raises the digestibility of the straw. Especially, the 2nd cut of IR73111-B-R-15-3-1 line was similar to the control at the TDN yield of straw, and would be used as a breeding material for product the roughage of good quality.

• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.353-360
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• 2009

When 14 microsatellite (MS) markers were applied in the identifying test for 480 Hanwoo, the discriminating power was estimated as $3.43{\times}10^{-27}$ based on the assumption of a random mating group (PI). This rate is 1,000 times higher than that of 60 single nucleotide polymorphism (SNP) markers. On the other hand, the power of the 60 SNP markers was estimated as $4.69{\times}10^{-20}$ and $8.02{\times}10^{-12}$ on the assumption of a half-sib mating group ($PI_{half-sibs}$) and a full-sib mating group ($PI_{sibs}$), respectively. These powers were 10 times and 10,000 times higher than those of the 14 MS markers. The results indicated that the total number of alleles (MS vs SNP = 146 vs 120) acted as a key factor for the discriminating power in a random mating population, and the total number of markers (MS vs SNP = 14 vs 60) was a dominant influence on the power in half-sib and full-sib populations. In the Hanwoo population, in which it was assumed that the entire population is the enormous half-sib group formed by the absolute genetic contribution of a few nuclear bulls, there will be only a 10 times difference in the discriminating power between the 14 MS markers and the 60 SNP makers. However, the probability of not excluding a candidate parent pair from the parentage of an arbitrary offspring, given that only the genotype of the offspring ($PNE_{pp}$) was 1,000 times higher as shown by the 14 MS markers than that by the 60 SNP markers. The strong points of SNP makers are the stability of the variation (low mutation rate) and automation of high-throughput genotyping. In order to apply these merits for the practical and constant Hanwoo identity test, research and development are required to set a cost-effective platform and produce a homemade apparatus for SNP genotyping.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.12-16
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• 2015

This study was carried out to evaluate accurately physical characteristics of total mixed rations (TMR) by investigating the effects of physically effective neutral detergent fiber content on the growth performance and carcass characteristics in fattening steers. Twelve Hanwoo (Bos Taurus coreanae) steers aged 26 months were used in this trial. Steers were offered one of two total mixed ration (TMR) diets - High or Low physically effective neutral detergent fiber (peNDF) content groups-that were differentiated by mixing time. The carcass traits of the experimental animals were evaluated by Korea Institute for Animal Products Quality Evaluation (KAPE) after slaughter. Body weight gain during the experimental period was significantly higher (P<0.05) in the High group ($30.00{\pm}10.27kg$) compared with the Low group ($17.20{\pm}6.34kg$), and daily weight gain was also high in the former. Although there was no significant difference, the carcass weight was higher in the High group ($414.60{\pm}34.28kg$) compared with the Low group ($377.80{\pm}11.97kg$). However, the beef quality grade of the Low group ($1^{++}$) was higher than that of the High group ($1^+$). Increases in body weight gain, daily gain, and carcass weight for the High group were mainly influenced by a high level of dry matter (DM) intake and digestibility; consequently, back-fat thickness increased and the beef quality grade was lowered. Because growth performance and carcass characteristics were affected by the physical traits of the diet, we suggested that proper peNDF content is also needed for fattening steers.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.4
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• pp.227-234
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• 2019

This study was performed to investigate the effect of heat-stressed environment on rumen microbial diversity in Holstein cows. Rectal temperature and respiration rate were measured and rumen fluid was collected under normal environment (NE; Temperature humidity index (THI)=64.6) and heat-stressed environment (HE; THI=87.2) from 10 Holstein cows (60±17.7 months, 717±64.4 kg) fed on the basis of dairy feeding management in National Institute of Animal Science. The rumen bacteria diversity was analyzed by using the Illumina HiSeq^TM 4000 platform. The rectal temperature and respiratory rate were increased by 1.5℃ and 53 breaths/min in HE compared to that in NE, respectively. In this study, HE exposure induced significant changes of ruminal microbe. At phylum level, Fibrobacteres were increased in HE. At genus level, Ruminococcaceae bacterium P7 and YAD3003, Butyrivibrio sp. AE2032, Erysipelotrichaceae bacterium NK3D112, Bifidobacterium pseudolongum, Lachnospiraceae bacterium FE2018, XBB2008, and AC2029, Eubacterium celulosolvens, Clostridium hathewayi, and Butyrivibrio hungatei were decreased in HE, while Choristoneura murinana nucleopolyhedrovirus, Calothrix parasitica, Nostoc sp. KVJ20, Anabaena sp. ATCC 33047, Fibrobacter sp. UWB13 and sp. UWB5, Lachnospiraceae bacterium G41, and Xanthomonas arboricola were increased in HE. In conclusion, HE might have an effect to change the rumen microbial community in Holstein cows.