• Korean Journal of Breeding Science
• /
• v.42 no.1
• /
• pp.40-49
• /
• 2010

This study was conducted to examine genetic variation on leaf characteristics of Zelkova serrata populations. Leaf samples were obtained from eighteen populations and fourteen leaf characteristics such as leaf blade length, leaf width, leaf area and etc. were measured. In all leaf characteristics, there were significant differences among populations and among individuals within population. Most variance of leaf characteristics was contributed to among individuals within population except for length from leaf base to maximum width (x3) and the ratio of x3 to leaf blade length (x10). The relatively high variations of leaf characteristics were found at Gurye, Jungeup and Gyungju populations while Youngwol, Cheongsong, Youngchun populations showed the lower variation. There was high correlation among leaf characteristics related to leaf size, but not among the variables of ratio between leaf characteristics. Length from leaf base to maximum width(x3) and the ratio of x3 to x10 showed significant positive correlation with latitude and altitude of populations, which reflect the differences of mean annual temperature among populations. Mean annual rainfall of populations showed negative correlation with leaf blade length, leaf width, length from apex to first serration and leaf area. Four principal components (PC) were deduced from principal component analysis, which explain the 88.5% of total variance of leaf characteristics. Leaf area, length from leaf base to maximum width, serration number and petiole length showed the highest contribution to PC1, PC2, PC3, PC4, respectively. According to cluster analysis, the populations of Z. serrata were divided into two groups, which reflect the difference of mean annual temperature between groups. Within group, however, specific tendency of clustering was not observed among populattions.

• Journal of Korean Society of Forest Science
• /
• v.97 no.4
• /
• pp.431-436
• /
• 2008

7 populations of rare endemic Berchemia berchemiaefolia were analysed using multivariate analysis for 9 characteristics of leaf morphology. The aim of this study was to examine geographic variation to support a genetic resource conservation plan of B. berchemiaefolia in Korea. In the morphological characters, nine characters of leaf were 10.25 cm (blade length), 4.10 cm (maximum width), 2.52 (blade length/maximum width), 3.22 cm (upper 1/3 width), 3.42 cm (lower 1/3 width), 0.95 (upper 1/3 width/lower 1/3 width), 1.24 cm (petiole length), 8.91 (blade length/petiole length), 8.16ea (vein number), respectively. Nested anova showed that were statistically significant differences among populations as well as among individuals within populations in all 9 quantitative characters. In 7 of 9 characters, variance components among individuals within populations were higher than those among populations. Cluster analysis using complete linkage method showed two groups (Chungbuk and Gyeongbuk districts) to Euclidean distance 1.2. Among principal components, primary 3 principal components appeared to be major variables because of the loading contribution of 87.3%. The first contribution was blade length, blade length/maximum width and blade length/petiole length; the second one was maximum width, upper 1/3 width and lower 1/3 width; the third one was petiole length, respectively.

• Journal of Bio-Environment Control
• /
• v.27 no.3
• /
• pp.222-230
• /
• 2018

This study aimed to determine effects of light-emitting diodes on plant growth, leaf morphology and cell elongation of two cultivars ('World-star' and 'Sushiro') of Spinacia oleracea. Plants were grown in a NFT system for 25 days after transplanting (DAT) under the LEDs [White (W), Red and Blue (RB, ratio 2:1), Blue (B), Red (R) LED] under the same light intensity and photoperiod ($130{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 hours). The 'World-star' variety was significantly higher in shoot fresh and dry weights, leaf number, and leaf area than the 'Sushiro' variety. For the 'World-star' variety, the two treatments of mixed light (RB) and red light (R) showed a 35% higher shoot dry weight than that of blue light (B) and white light (W) at 25 DAT. In the 'Sushiro' variety, mixed light (RB) treatment, which had the highest shoot fresh and dry weights, showed 40% higher than the white light (W) treatment, which had the lowest shoot fresh and dry weights. Both varieties showed leaf epinasty symptom at 21 DAT only in both mixed light (RB) and red light (R), and red light (R) treatment showed significantly higher symptom than mixed light (RB), indicating the leaf epinasty is associated with red light. Microscopic observations of the cell size in the leaf center and edge parts showed that the cell density of leaf edge under the red light (R) was lower than that in leaf center, supporting previous reports that suggest an association of the cell size difference between the leaf center and edge with the leaf epinasty occurrence. Since the blue light (B) plays a role in alleviating the epinasty symptom caused by the red light (R), it seems necessary to identify the appropriate mixing ratio of the two light sources. In addition, the World-star variety seems to be more suitable for the cultivation of plant factory using LED light sources.