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Simple Mass-screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici (토마토 시들음병에 대한 간편 대량 저항성 검정법)

  • Park, Myung Soo;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.110-116
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    • 2013
  • This study was carried out to establish the simple mass-screening methods for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). Root dip inoculation method has been used in many studies on the resistance of tomato to disease. On the other hand, in mass-screening for resistant tomato to Fusarium wilt, the inoculation method is time-consuming and laborious procedure. Disease development of two FOL isolates on two cultivars of tomato according to inoculation method including root dip, tip and scalpel methods were investigated. In compatible interaction, tomato seedlings of each cultivar inoculated by tip method showed the lower and more variable disease severities than by root dip method. Whereas the seedlings by scalpel method represented clear resistant and susceptible responses to Fusarium wilt as root dip method. The resistance degree of each cultivar inoculated with FOL isolates by scalpel method was hardly affected by the tested incubation temperature and inoculum concentration. On the basis of the results, we suggest scalpel inoculation method as an efficient mass-screening method for resistant of tomato cultivars to Fusarium wilt. Roots of tomato seedlings at two-leaf stage grown in plastic cell tray were injured with scalpel and then spore suspension (more than $1{\times}10^7\;conidia{\cdot}mL^{-1}$) of FOL was poured directly on the roots. The infected plants were cultivated in a growth room at $25-30^{\circ}C$ for 4 weeks with 12-hours light a day.

Study on No-tillage Silage Corn Production with Legume Hairy Vetch (Vicia villosa Roth) Cover II. Changes of yield and nitrogen upake of corn by N fertilizer and hairy vetch cover (헤어리베치 피복을 이용한 옥수수 무경운 재배에 관한 연구 II. 질소시비 및 헤어리베치 피복에 의한 옥수수의 수량 및 질소 흡수량의 변화)

  • 서종호;이호진
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.18 no.2
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    • pp.123-128
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    • 1998
  • Legume winter cover crop hairy vetch(Vicia villosa Roth, HV) can supply mineral nitrogen for silage corn by HV cover killed. The purphose of this study was to understand changes of soil mineral N, yield and N uptake of silage corn by N level (0, 135kgFN/ha) and cover cmp(no cover crop: NCC, hairy vetch cover crop: HVC) at field of Crop Experiment Station in 1996. HV growth decreased soil mineral N concentration before seeding corn, but killed HV cover increased concentration of soil mineral N at surface soil (0~7.5cm) at six-leaf stage of corn. Total dry matter(DM) and N uptake of corn averaged over N level was more decreased in HVC than in NCC at silk stage, but N uptake of corn after silk was more increased in HVC than in NCC by N mineralized fiom HV killed, especially in OkgFNJha. N fertilization increased total DM and and N uptake of corn averaged over cover crop, especially more increased the DM and N uptake before silk stage. Early application of N fertilizer was recommendable in netillage silage corn using hairy vetch cover crop.

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Growth Analysis of Silage Corn in Response to Seeding Time (파종기 이동에 따른 Silage 옥수수의 생장해석)

  • 강정훈;이호진;박병훈
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.3
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    • pp.212-219
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    • 1985
  • The field experiment was conducted to study on the growth analysis of early variety, MTC-1, and late variety, Suweon 19, in response to seeding time at Suweon. The results summarized as follows: 1. Days required for Leaf-Formation(L-phase) of MTC-1 and Suweon 19 was same but that of Node-Thickening(N-phase) was quite different, e.g. late variety has two times longer. The period of Ear-Development (E-phase) in MTC-1 was 41 days and 53 days in Suweon 19. L-and N-phase were shortened by late seeding but E-phase was not influenced by seeding time. 2. Leaf Area Index(LAI) was decreased and Leaf Area Ratio(LAR) was increased by late seeding. Days required to maximum LAR was less by late seeding. Net Assimilation Rate(NAR) of L-, N-, and E-phase was decreased but its tendency in E-phase was remarkable at late seeding. 3. Crop Growth Rate(CGR) was increased gradually in the process of plant growth and it was the highest at Husk-stage. Relative Growth Rate(RGR) was increased in L-phase and was decreased in N- and E-phase, but its tendency in N-phase was great. 4. The period required to the maximum dry matter yield was shortened by late seeding and dry matter yield was increased by early seeding.

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Studies on Artificial Polyploid Forest Trees V -On Morphological Characteristics in Colchitetraploids Pinus densiflora (인위배수성임목(人爲倍數性林木)에 관(關)한 연구(硏究) V -Colchitetraploids Pinus densiflora의 외부형태(外部形態)에 대(對)하여)

  • Kim, Chung Suk
    • Journal of Korean Society of Forest Science
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    • v.7 no.1
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    • pp.19-27
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    • 1968
  • As a preliminary investigation to obtain useful auto- and allopolyploids a ten year old tetraploid of Pinus densiflora induced from the colchicine treated seed was observed on the cytological, morphological and physioa logical characters in the present study and the results can be summarized as follows. 1. The number of chromosome at the base of needle was 48 so that it was recognized as a tetraploid. 2. The needles were thicker and the number of them on an individual was less than the 2n plant. The needle combined to a single was appeared 2%. 3. No difference was found in the number of stomata rows on the central part of needle between the tetraploid and 2n plant, however, guard cells of tetraploid increased 36% in the long diameter compared with 2n plant. 4. Microsporangiate storbile developed normally showing the same size with 2n plant while the size of pollen grain increased about 20% larger than 2n plant. 5. Germination percentage in vitro increased until 17 hours but decreased after 26 hours compared with 2n plant. The longest length of pollen tube during the germination period appeared in the tetraploid pollen.

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Sap Outflow Characteristics of Walnut Tree (Juglans sinensis Dode) (호두나무 수액의 유출특성)

  • Kim, Chul-Woo;Kim, Mahn-Jo;Park, Youngki
    • Korean Journal of Plant Resources
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    • v.27 no.2
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    • pp.188-193
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    • 2014
  • The sap outflow characteristics of Juglans sinensis and J. mandshurica were investigated to evaluate the optimum pruning period of walnut tree that there is a sap spill on dormant. The total period of sap outflow were 34 days for both J. sinensis and J. mandshurica. Total amount of sap outflow per tree in J. sinensis and J. mandshurica were 2,922 mL/tree and 3,135 mL/tree, respectively and the period of sap outflow and non sap outflow between two species were similar. ANOVA analysis showed that the amount of sap outflow was significant differences with day of sap outflow but there were no significant differences between species. From the correlation analysis between air-temperature and sap outflow, daily minimum temperature showed a positive correlation at the 1% level of significance (r=0.56 and r=0.46) for both J. sinensis and J. mandshurica. When branch of walnut tree that diameter is 5 cm cut on the period of sap outflow, the sap flowed down the longest period (48 days) but the sap outflow was not observed after budburst. Therefore, our study supported that the pruning have to avoid the period of sap outflow to reduce sap outflow of walnut tree.

Influence of Diagnostic Fertilization and Subsoil Breaking on Soil physico-chemical Properties in Direct Seeding of Rice on Flooded Paddy Surface (벼 담수표면 직파재배시 진단시비와 심토파쇄가 토양이화학성 및 벼 생육에 미치는 영향)

  • Yoo, Chul-Hyun;Ryu, Jin-Hee;Yang, Chang-Hyu;Kim, Taek-Kyum;Kang, Seung-Weon;Kim, Jae-Duk;Jung, Kwang-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.6
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    • pp.334-338
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    • 2006
  • This study was conducted to evaluate the effect of improvement of soil physical properties such as deep plowing, subsoil breaking and diagnostic fertilization on the yield of rice and nitrogen-use efficiency in direct seeding on flooded paddy surface of rice. The effects of deep plowing, subsoil breaking and diagnostic application of N, P, K fertilizers, Latex coated urea(LCU), compost, silicate were investigated. The soil physical properties, such as bulk density, hardness and porosity were improved and the content of organic matter and available $SiO_2$ were also increased by deep plowing and subsoil breaking. The amount of $NH_4-N$ in soil was highly increased by diagnostic fertilization and deep plowing at 5th leaf stage. The nitrogen-use efficiency was the highest at the diagnostic application of LCU 70% applied as basal dressing with subsoil breaking. The yield of rice increased by 8% under the diagnostic application of LCU 70% applied as basal dressing with subsoil breaking compared with the conventional application.

Study of germination for none seasonal pruning mulberry farm and their insective diseases (순상전 춘기말벌채고조의 발아상태와 충해 발생 관계)

  • 도경화
    • Journal of Sericultural and Entomological Science
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    • v.3
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    • pp.13-21
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    • 1963
  • 1. In the mulberry groves for both rearing of spring and fall, the rate of germination was 74.3 percent and the rate of the unfinished bud was 25.7 percent. 2. When the summer pruning after spring rearing was not worked, new sprouted leaves from the axillary buds of an age old branches was 74.88 percent of germination and the rest from unfinished bud during the spring showed 28.12 percent. 3. In the furrows running from north to south, new buds facing the due north were the worst but towards south-east and north-east were the best in their sprouts. 4. One year-old branches which had been raised without the summer pruning in the bush types of Morus Lhou (ser) Koidz offered a habitat where Apriona Rugico1lis Cherr could live. 5. On the grove of Morus Lhou Koidz cultivated at lower zone under a higher humidity and the shortage of sun light the appearance of Anomoneura Mori Schw was likely to happen very much. 6. One year old branches without the summer pruning produced more leaves owing to a long period of growth than branches with the summer pruning in the fall crops. 7. It is necessary to reconsider a certain distance of planting because of causing, when the summer pruning was not made in the bush plantation, a obstacle of fertilizer operation or the forth coming year's management. 8. It can not be thought the ideal method for us to raise mulberry tree such as Morus Lhou Koidz under natural form and at a short distance of planting (one another). 9. Among the species, Morus alba L. had a great deal of leaves and of the rate of germination per unit length of branch.

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Characteristics of mulberry cultivar 'Sangmaru' (Morus alba L.) for mulberry fruit production (오디 생산용 뽕나무 품종 '상마루'의 특성)

  • Sung, Gyoo Byung;Kim, Yong Soon;Kim, Kee Young;Ji, Sang Duk;Kim, Hyun-bok
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.110-117
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    • 2015
  • We bred a mulberry cultivar named Sangmaru, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at three places (Suwon, Cheongju, and Jangseong) for seven years from 2008. This is tetraploidy variety belonging to Morus alba L. made by colchicine treatment on growing point of 'Chungil (Morus alba L.)' winter buds. Sangmaru was high yielding cultivar in fruit productivity by 6% compared to control cultivar 'Chungil (Morus alba L.)' for five years. Although Sangmaru was similar in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

Characteristics of Mulberry Cultivar "Sangberry"(Morus alba L.) for Fruit Production (오디 생산용 뽕품종 "상베리"의 특성)

  • Sung, Gyoo Byung;Kim, Hyun-Bok;Kang, Pil Don;Kim, Ki Young;Ji, Sang Duk
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.171-177
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    • 2012
  • We bred a mulberry cultivar named Sangberry, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four places(Suwon, Kongju, Jangseong and Sangju) for six years from 2005. This is tetraploidy variety belonging to(Morus alba L.) made by colchicine treatment on growing point of winter buds. Sangberry was high yielding cultivar in fruit productivity by 70% compared to control cultivar "Chungilppong(Morus alba L.)" for four years. Although Sangberry was a little lower in sugar content of mulberry fruits, it contains more bioactive materials like C3G, rutin and amino acids than Chungilppong. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

The Legend's Modernization and Horror Strategy - Focus on uncanny stories concerning army (전설의 현대화와 공포의 전략 - 군대괴담을 중심으로 -)

  • Jin, Eun-Jin
    • (The)Study of the Eastern Classic
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    • no.68
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    • pp.439-472
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    • 2017
  • The purpose of this paper is to confirm how traditional legends are changing and existing in modern times, and how they are inheriting and developing legendary values and meanings. The subject of the study is uncanny stories concerning army that is judged to be worthwhile alongside uncanny stories concerning school. The uncanny stories concerning army are urban legends that are active in the internet. It can be defined as 'a narrative story that raises fear with supernatural and strange experiences in the army'. In addition to the internet community sites, humor sites and game sites are actively handed down. Recently, ghost stories have appeared on the site, and uncanny stories concerning army are easily collected from these various sites. The uncanny stories concerning army can be divided into three types: ghost experience type, Bulgogi GP type, mystery radio type. The ghost experience type has the largest number of version, the contents are rich and varied. It does not deviate much from the traditional notion of ghosts. Bulgogi GP type has a frenzied horror from its name. It is related to actual events and raises horror. Mystery radio type is a romantic material used in movies and dramas. However, unlike movies and dramas, uncanny stories concerning army are perceived as fearsome. The uncanny stories concerning army continues to emphasize that the story is actually experienced in the title and content. It has presented concrete evidence. The emphasis on truthfulness is intended to make fear more realistic. This comes from the desire for safety. Uncanny stories concerning army focus on horror. So It tries to reveal the history and truthfulness of the story itself. As a result, the history of reality, the truth of reality, and the realistic ideology are erased. Historical and truthfulness of traditional legends has been varied in the case of uncanny stories concerning army.