• Textile Coloration and Finishing
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• v.12 no.3
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• pp.199-206
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• 2000

Silk fabric was dyed using methanol extract of Chrysanthemum boreale at $60^\circ{C}$ for 40min. The K/S value of the silk fabric was highest in the premordanting method, while the amount of absorbed mordant was largest in the Cu-mordanting. The surface colors of the dyed fabric highly depended upon mordants or mordanting methods. For all cases, the value of the dyed fabric was dark. The chroma was high by using the Cr-mordant and the color difference was distinct by using the Fe-mordant. Cu-mordanted silk showed highest fastness to light. Dyed silk fabrics by mordanting method showed good antimicrobial activity ad deodorization property.

• Korean Journal of Animal Reproduction
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• v.21 no.1
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• pp.39-46
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• 1997

본 연구는 체외생산된 소 배반포기배의 inner cell mass (ICM) 세포에서 epidermal growth factor-receptor (EGF-R)의 발현 유무를 immunosurgery와 indirect immunofluorescence (간접 면역 형광방법)을 이용하여 조사하고자 실시하였다. 본 실험에 사용된 ICM 세포는 체외수정 후 7∼8일째에 회수된 소 배반포기배로부터 immunosurgery 방법을 실시하여 얻어졌으며, 회수된 ICM세포는 live/dead 염색방법을 통한 생사 유무와 EGF-R 발현 유무 조사에 공시되었다. 특히, 배반포기배에 대한 immunosurgery를 위해 trophectoderm 세포에 대한 rabbit anti-bovine trophectoderm cell antibody (RABTE)를 제조하여 사용하였다. 결과를 요약하면 다음과 같다. ICM세포의 회수율은 RABTE와 guinea pig serum (complement)에 각각 15∼30분과 15∼60분동안 처리했을 경우 16.7∼74.2%였으며, 또한 처리시간이 각각 30분과 30분일 때 가장 높은 회수율(74.2%)을 얻었다. Immunosurgery 후 얻어진 ICM세포의 생존 유무를 조사하기 위해 live/dead 염색 방법을 이용하였던바, ICM세포의 생존율은 complement가 60분 처리된 군(69.3%)을 제외한 모든 처리군에서 84.0∼91.6%의 높은 생존율을 나타냈다. 또한, 회수된 ICM세포에 대한 EGF-R의 존재를 확인하였다. 따라서, ICM세포에서의 EGF-R의 발현은 인위적으로 첨가된 EGF의 이용 가능성을 높임으로서 체외에서의 착상전 배 발달을 증진시킬 수 있을 것으로 사료된다.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2003.04a
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• pp.157-161
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• 2003

최근 들어 사회가 고도화 ㆍ 다양화됨으로 인해 관련 산업 전분야로 컴퓨터 시스템의 보급은 급속도로 확산되고 있다. 특히, 섬유 날염 분야에도 다품종 ㆍ 소롯트 추세에 대응하기 위한 컴퓨터 시스템을 응용한 디지털 날염시스템의 다양한 기계 장치와 관련 소재 개발이 구미 선진국을 중심으로 활발히 진행되고 있는 것이 세계적인 현상이다. 이에 본 연구에서는 수년전부터 국내 날염 업계와 제품 디자인 업계에 상당수 보급되어 운영되고 있는 다지널 날염머신에 필요한 천연섬유소재와 합성섬유소재 등 디지털 날염전용 섬유소재원단을 개발하고자 한다. (중략)

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2009.11a
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• pp.141-142
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• 2009

환경친화섬유 대나무, 옥수수, 콩, 바나나, 종이섬유 5종의 특징과 이를 원료로 한 필라멘트사 2종과 방적사 5종을 사용하여 제조된 직물의 항균성, 소취성, 보온성, 흡수성 등에 대한 성능평가를 실시하여 제품별 기능성 평가 자료를 정리하였다.

• Proceedings of the Korean Society of Community Living Science Conference
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• 2004.05a
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• pp.175-176
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• 2004

탱자나무는 낙엽활엽소교목으로 중국이 원산지며, 우리나라에서는 경기이남 지역에 자생하고 있다. 과거에 탱자나무의 날카로운 가시로 인해 도적을 막고 귀신을 쫓기 위하여 울타리용으로 많이 심었다. 한의학에서는 지실이라고도 하며 건위ㆍ이뇨ㆍ거담ㆍ진통 등에 쓰인다. 그리고 차로도 음용하며 익은 탱자를 지각이라 한다. 최근 들어 탱자가 아토피 피부에 효능이 있다는 보고에 따라 탱자추출물의 염색성을 검토해 보았다. (중략)

• Journal of Veterinary Clinics
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• v.15 no.1
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• pp.196-198
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• 1998

젊은 말과 그 관리인에게 발생한 Microsporum canis 감염에 의한 백선에 대해 보 고하였다. 말과 사람에 있어서의 진단은 피부 병변의 KOH 표본에서 피부사상균을 직접 증명 하고,감염된 인설의 순수 배양물로부터 Microsporum canis를 분리한 것에 근거를 두었다. 말과 사람에 서 분리하고, PHOL 염색액으로 염색한 Microsporum canis는 현미경으로 자세히 관찰했을 때 형태학 적인 차이를 나타내지 않았다. 말에 있어서 옥도정기와 사람에 있어서 miconazole에 의한 국 소 요법은 효과적이었다. 병력은 관리인이 말로부터 감염된 것을 제시하였다.

• Clinical and Experimental Pediatrics
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• v.51 no.10
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• pp.1112-1117
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• 2008

Purpose : We intended to observe cell death and apoptotic changes in neurons in organotypic hippocampal slice cultures following oxygen-glucose deprivation (OGD), using propidium iodide (PI) uptake, Fluoro-Jade (FJ) staining, TUNEL staining and immunofluorescent staining for caspase-3. Methods : The hippocampus of 7-day-old rats was cut into $350{\mu}m$ slices. The slices were cultured for 10 d (date in vitro, DIV 10) and and exposed to OGD for 60 min at DIV 10. They were then incubated for reperfusion under normoxic conditions for an additional 48 h. Fluorescence of PI uptake was observed at predetermined intervals, and the cell death percentage was recorded. At 24 h following OGD, the slices were Cryo-cut into $15{\mu}m$ thicknesses, and Fluoro-Jade staining, TUNEL staining, and immunofluorescence staining for caspase-3 were performed. Results : 1) PI uptake was restricted to the pyramidal cell layer and DG in the slices after OGD. The fluorescent intensities of PI increased from 6 to 48 h during the reperfusion stage. The cell death percentage significantly increased time-dependently in CA1 and DG following OGD (P<0.05). 2) At 24 h after OGD, many FJ positive cells were detected in CA1 and DG. Some neurons had distinct nuclei and processes while others had fragmented nuclei and disrupted processes in CA1. TUNEL and immunofluorescent staining for caspase-3 showed increased expression of TUNEL labeling and caspase-3 in CA1 and DG at 24 h after OGD. Conclusion : The numerous dead cells in the slice cultures after OGD tended to display apoptotic changes mediated by the activation of caspase-3.

• Textile Coloration and Finishing
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• v.11 no.1
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• pp.9-15
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• 1999

Using water and methanol, the colorant components were extracted from the heartwood of amor cork tree which is known as the oldest natural yellow dye. The dyeabilities of the colorant on cotton and the fastness of cotton fabrics dyed with amur cork tree extracts were investigated. According to the dye uptake and mordant concentration, antibacterial activities and deodorization properties of dyed fabrics were also investigated. As a results, K/S values of dyed fabrics were increased with concentrations of mordants and amur cork tree extracts. Washing fastness of dyed fabrics was very low, light fastness was fair and the other fastness were good. Deodorization rates of dyed fabrics were increased in proportion to concentrations of amur cork tree extracts. The reduction rates of bacteria measured by Bioassay tests were also increased in proportion to concentrations of mordants and amur cork tree extracts.

• Textile Coloration and Finishing
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• v.23 no.1
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• pp.21-27
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• 2011

The natural dyeing of cotton fabric with gardenia blue powder was studied. Cotton fabrics were treated with chitosan in order to increase K/S values and colorfastness. K/S values were increased with increasing the concentration of gardenia blue powder, dyeing time and temperature, and the lower pH of dye bath. In case of chitosan finishing, K/S values were increased, and ${\lambda}_{max}$ shifted to 600nm at 400nm. The wash fastness was improved, but the abrasion fastness was lowered in the case of wet rubbing.

• Textile Coloration and Finishing
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• v.12 no.5
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• pp.301-307
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• 2000

Silk fabrics were dyed with Gromwell extracts after mordanted with various mordants and their dyeability, antibacterial.deodorization activity, fastness and change of surface color were investigated. Silk fabrics were mordanted at $60^\circ{C}$ for 30 minutes and subsequently dyed at $40^\circ{C}$ for 30 minutes. The dye-uptake of silk fabrics increased with the concentration of Gromwell extracts and mordants. Surface color of silk fabrics changed differently according to the type of mordants used. Antibacterial and deodorization activities of silk fabrics dyed with Gromwell extracts were good. Cu mordant was more effective for deodorization activity. Dyed silk fabrics showed poor light fastness but good dry-cleaning and perspiration fastness.