• Title/Summary/Keyword: 염기

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Effect of Excessive Basic Amino Acids in Supplemented Diet on the Weight Gain and Blood Urea Nitrogen Concentration of Mice (염기성 아미노산의 과잉 투여가 흰쥐의 체중 및 혈액 중의 Urea Nitrogen 농도에 미치는 영향)

  • KIM Soon-Seon;PARK Yeung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.15 no.1
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    • pp.52-58
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    • 1982
  • The effects of casein diet supplemented with excessive levels of three basic amino acidslysine, histidine and arginine - were studied in male, weanling, I. C. R. strain mice. The relationship between weight gain and concentration of blood urea nitrogen was also discussed with the follows results : 1. The groups supplemented with basic amino acids were demonstrated depressed growth and food intake than the control ($10\%$ casein diet) group, $5\%$ L-histidine HCl supplemented group being most depressed. 2. The concentration of blood urea nitrogen in the basic amino acid supplemented groups were higher than the control group. The value of L-arginine supplemented group was highest. 3. The concentration of blood urea nitrogen was related to the amounts of nitrogen contained in the diet not related to the growth gain.

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Practical Use of DNA Polymorphisms in the Avian Immunoglobulin Light Chain Constant Domain for Species-specific PCR (조류의 종 특이 구별을 위한 항체 유전자의 이용)

  • Choi, J.W.;Kang, S.J.;Park, M.S.;Kim, J.-K.;Han, J.Y.
    • Journal of Animal Science and Technology
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    • v.50 no.1
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    • pp.9-18
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    • 2008
  • Species-specific polymorphisms in chicken, pheasant, turkey, and quail were identified by cloning and sequencing of the immunoglobulin constant domain (IgLC). A set of species-specific primers were then designed on the basis of polymorphisms in the IgLC between species, as well as two additional sets of primers for the cytochrome b and tapasin genes, for the purpose of species identification. Together, the primers successfully distinguished specific species from chicken by species-specific PCR. This simple but unambiguous method may be used to screen avian inter-species germline chimeras, which are valuable models for the conservation of endangered species.

A Classifier for the association study between SNPs and quantitative traits (SNP와 양적 표현형의 연관성 분석을 위한 분류기)

  • Uhmn, Saangyong;Lee, Kwang Mo
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.11
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    • pp.141-148
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    • 2012
  • The advance of technologies for human genome makes it possible that the analysis of association between genetic variants and diseases and the application of the results to predict risk or susceptibility to them. Many of those studies carried out in case-control study. For quantitative traits, statistical analysis methods are applied to find single nucleotide polymorphisms (SNP) relevant to the diseases and consider them one by one. In this study, we presented methods to select informative single nucleotide polymorphisms and predict risk for quantitative traits and compared their performance. We adopted two SNP selection methods: one considering single SNP only and the other of all possible pairs of SNPs.

Petrological Study on the Intermediate-basic Plutonic Rocks in the Southwestern Part of the Korean Peninsula (한반도 서남부에 분포하는 중성-염기성 심성암류에 대한 암석학적 연구)

  • Kim, Yong-Jun;Park, Jae-Bong;Park, Byung-Kyu
    • Journal of the Korean earth science society
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    • v.27 no.5
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    • pp.528-538
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    • 2006
  • Main aspect of this study is to clarify the petrochemistry and petrogenesis of intermediated-basic plutons located in the southwestern part of the Korean peninsula. These Intermediated-basic plutons consist of Pre-Cambrian anorthosite-gabbro, Triassic hornblende gabbro (Jirisan area), Jurassic diorite-syente (Jirisan and north area) and Cretaceous gabbro-diorite (south area). The Massif type anorthosite has multi intrusions, where each one intruded by gabbroic rocks, composed of gabbro, norite, troctolite and leucogabbro. In the variation diagram of the major-minor composition, AMF and Pl-Px-Ol diagrams, we suggest that intermediated-basic plutons in the southwestern part of the Korea show a trend consistent to Daly's value and calc-alkaline rock series. Accoding to REE (La/Yb)cw and Eu/Sm, these plutons are enriched with LREE than HREE, and emplaced by the tectonic setting in continent and/or continental margin.

Identification of Metarhizium sp. Isolated from Protaetia brevitarsis seulensis (Kolbe) Using Ribosomal DNA Sequence (흰점박이꽃무지로부터 Metarhizium속 사상균의 분리 및 ribosomal DNA 염기서열에 의한 동정)

  • 최지영;김철학;제연호;최영철;김종길;박규택;김근영
    • Korean journal of applied entomology
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    • v.42 no.1
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    • pp.65-70
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    • 2003
  • For the purpose of the protection of beneficial insects from pathogens and the development of control agent against pests, a strain of Metarhizium sp. was isolated from the infected Protaetia brevitarsis seulensis larvae in Korea. Under the scanning electron microscope, the isolate, Metarhizium sp. KMA-1, showed distinct formation of conidia on the palisade-like masse which were comprised of elongate chains and this shape is a typical feature of Metarhizium species. PCR techniques were used to identify the isolate and the primers used were designed on the basis of two kinds of rRNAs sequences, 28S rRNA and internal transcribed spacer(ITS). The specific PCR products from each primer set were amplified and the DNA sequences were determined for the similarity comparison. Sequence alignment of these fragments using GenBank database resulted in the highest homology similarity between the isolate Metarhizium sp. KMA-1 and M. anisopliae. From these results, the isolate Metarhizium sp. KMA-1 in this study was identified as M. anisopliae.

Micellar Effect of the Aquation and the Base Hydrolysis of cis-[Co(en)2NH3Cl]2+ Ion (cis-[Co(en)2NH3Cl]2+ 이온의 수화반응과 염기성 가수분해반응에 미치는 미셀의 효과)

  • Jeong, Jong Jae;Baek, Seong O;Lee, Jeong A
    • Journal of the Korean Chemical Society
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    • v.38 no.4
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    • pp.265-270
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    • 1994
  • The aquation and base hydrolysis of [Co(en)$_2$NH$_3$Cl]$^{2+}$ were studied by UV spectroscopic method in various SDS aqueous solution. The base hydrolysis of [Co(en)$_2$NH$_3$Cl]$^{2+}$ with the addition of 0, 0.05, 0.1 mol dm$^{-3}$ sodium chloride was studied. For the aquation of the complex, the rate constant in the micellar phase(kH$^M$) was a little larger than that in the aqueous phase(kH$^W$). With the increase of SDS concentration, the second order rate constant(kOH) for the base hydrolysis unchanged below the CMC and sharply decreased down to a limiting value after the CMC was reached. The effect of added NaCl on the rate behavior of the complexes in the micellar solution were investigated by using an ion-exchanged model.

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Genetic relationship of Aloe vera 'Saengjang', a new forma, based on cpDNA and ITS sequence variation (cpDNA와 ITS 염기변이에 근거한 신품종 생장알로에 유전적 상관관계)

  • Srikanth, Krishnamoorthy;Jang, Seon Il;Whang, Sung Soo
    • Korean Journal of Plant Taxonomy
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    • v.44 no.4
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    • pp.250-256
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    • 2014
  • This study was carried out to understand the genetic relationship of three Aloe spp. cultivated in Korea, A. saponaria, A. vera and A. arborescens and a new variant in Korea based on three plastid (matK, trnL-F, rbcL) and one nuclear (ITS regions) DNA barcode markers. A total of 2,420 bp sequence was amplified. Two indels were detected in the trnL region, and also several species specific nucleotide loci were detected in all 29 parsimonious informative sites, and 148 variable sites were detected among four taxa studied while 170 variable and 75 parsimonious sites were detected when other Aloe spp. in worldwide were used. An UPGMA phenogram with 10,000 bootstrap replication showed that the new variant was closest to A. vera. The variant was not morphologically and genetically concurrent with any reported species so far. The clustering of Aloe species were broadly in agreement with previously reported results.

Effects of Basicity on the Carbonation Characteristics of Alkali-Activated Slag Mortar (염기도가 알칼리 활성고로슬래그 모르타르의 탄산화에 미치는 영향)

  • Song, Keum-Il;Lee, Bang-Yeon;Hong, Geon-Ho;Gong, Min-Ho;Song, Jin-Kyu
    • Journal of the Korea Concrete Institute
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    • v.24 no.5
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    • pp.577-584
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    • 2012
  • Carbonation resistance is one of the most influencing factors on durability of concrete. Alkali activated slag (AAS) is known to have weaker resistance for carbonation than OPC due to the low calcium contents. In this paper, the carbonation characteristic of AAS mortar which is related to the basicity (CaO/$SiO_2$) was investigated. In order to give the various basicity conditions, SM (source material) was blended with quicklime (CaO) and silicon dioxide ($SiO_2$) by adopting mechano-chemical treatment method. Experiments including flow test, compressive strength test, carbonation depth test, together with XRD, FTIR and TGA were employed to evaluate the effects of basicity of SM on the carbonation characteristics. The test results showed that the carbonation resistance effectively increased with the increase of the basicity of SM.

Flavor Components in the Squid Processing (오징어 가공중의 향기성분)

  • LEE Jong-Ho;CHOI Byeong-Dae;LEE Kang-Ho;RYU Hong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.22 no.5
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    • pp.370-374
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    • 1989
  • Volatile components in natural and basic fraction of the steam distillation extraction method from squid during processing were analysed by GC and GC-MS equipped with a fused silica capillary column. Thirty eight compounds were identified; they were 31 compounds from neutral, 7 compounds from basic fraction. The main components flavor of squid were 3-methylthiophene, 2-methyl-2-hexanthiol, 1-penten-3-ol, 3-penten-2-ol, 3-ethyl-1,4-hexadiene, 1-hydroxy-2-propanone, hexenal and benzaldehyde etc.. Especially, (E, E)-3,-5-octadecanal were detected during the boiled. 2,5-dimethyl pyrazine, 2-ethyl-6-pyra-zine, 2,3,5-trimethyl pyrazine and 2-ethyl-3,5-dimethyl pyrazine basic compounds, which have respectively a burnt and roasted odor, are considered to be important for the characteristic basic fraction of squid.

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Phylogenetic Analysis of Agaricus blazei and Related Taxa by Comparing the Sequences of Internal Transcribed Spacers and 5.8S rDNA (Internal Transcribed Spacer와 5.8S ribosomal DNA의 염기서열 분석에 의한 Agaricus blazei와 근연종에 대한 계통분류학적인 연구)

  • 김기영;하명규;이태호;이재동
    • Korean Journal of Microbiology
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    • v.35 no.3
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    • pp.180-184
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    • 1999
  • Molecular spslemaucs of Agaricus species was investigated on the base of the sequences of the internal transcribed spaceriITS) regions in ribosomal DNA (rDNA). The sequences of the ITS region in 5 species and two group of Agaricus genus were resolved. In the phylogenetic trees. the species generally divided inlo two subclusters, refered to here as the group I and group 11. The group I consisted of Agaricus blazei ATCC 76739, Agarictrs blazei species cultivated in Korean hmings. Ago/-icus anmensis IMSNU 32049 and Agaricus can~pestris VPI-OKM 25665. Between Agaricus blazei NCC 76739 and the Agaricus blazei species cultivated in Korean farmings had the variation in lhe 5 nucleotide on the ITS regions. These varieties were presumed the variation by the geographic and cultivated conditions. In addition the subgroup of group I was formed by Agaricus arvensis LMSNU 32049 and Agaricus carnpests VPI-OKM 25665. The group IT included Agnrictrs bispoms CH 3004 and Agaricus pocillotor DUKE-J 173.

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