• Parasites, Hosts and Diseases
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• v.36 no.3
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• pp.183-190
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• 1998

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected ill view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. slnensis antigen iIi 28 kDa as a if-falactosidase fusion protein produced in EscherichiG coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies or a 30 base pair repeat and an additional 320 bases. The deduced amino acid seqiLence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis, C. sinensis adult worm RNA showed a positive reaction with the cloned gene Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed higtl specificity for diagnosis of clonorchiasis.

• Journal of the Korean Chemical Society
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• v.63 no.1
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• pp.56-65
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• 2019

This study investigated the perceptions of the relationship among the three acid-base models of chemistry teachers. In addition, we examined how the perception of the relationship between models affected on the interpretation of concepts in each model. To investigate teachers' perceptions and interpretations, a questionnaire and interviews were conducted for 24 chemistry teachers. As results, most of the chemistry teachers recognized the three models as cumulative extension relationships. The perceptions were related to the contents of textbooks. The perception of the relationship of these models influenced on interpretations of the models' acid-base concept. In this study, we suggested that science teachers need to be aware of diverse models' roles.

• Journal of the Korean Chemical Society
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• v.64 no.1
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• pp.30-37
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• 2020

The purpose of this study was to identify the problems faced by students in sub-microscopic representation of acid-base reactions. Herein, we selected 30 students of 12^th grade science classes, who had studied various acid-base models. In order to investigate the sub-microscopic representation ability of the students, we developed nine items related to various contexts, such as one type of solute and solvent, two types of solutes and solvent, cases with water as solvent or with nonaqueous solvents. For all items, we consistently observed lack of concept of chemical change. In context of aqueous and nonaqueous solutions, the frequency of lack of concept of chemical bonding was high if ammonia was the solute or solvent. Moreover, the frequency of lack of concept related to the degree of electrolytic dissociation was high. Therefore, chemistry teachers should understand that students' ability to sub-microscopic representation of acid-base reactions can be enhanced by analyzing the difficulties faced by the students in solving diverse acid-base problems.

• Applied Chemistry for Engineering
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• v.9 no.6
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• pp.920-923
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• 1998

In this work, the adsorption characteristics of the activated carbon treated with 30 wt. %HCl and 30 wt. % NaOH were investigated. The acid and base values were determined by Boehm's method and the surface area and porosity was measured by BET-method with $N_2$-adsorption. Also, the adsorption characteristics of the activated carbons treated with acid and base chemical solutions were investigated with $CO_2$ and $NH_3$-adsorption. From which, relatively different adsorption behaviors of the modified activated carbons were observed in the amounts of $CO_2$ and $NH_3$ adsorptions, even though the physical surface structures of the activated carbons, such as specific surface area, pore size and pore volume, were not significantly changed.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.734-738
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• 2005

A gene coding for xylanase from alkali-tolerant Bacillus alcalophilus AX2000 was cloned into Escherichia coli $DH5\alpha$ using pUC19. Among 2,000 transformants, one transformant showed clear zone on the detection agar plate containing oat-spells xylan. Its recombinant plasmid, named pXTY99, was found to carry 7.0 kb insert DNA fragment. When the nucleotide sequence of the cloned xylanase gene (xynT) was determined, xynT gene was found to consist of 1,020 base-pair open reading frame coding for a poly-peptide of 340 amino acids with a deduced molecular weight of 40 kDa. The coding sequence was preceded by a putative ribosome binding site, and the transcription initiation signals. The deduced amino acid sequence of xylanase is similar to those of the xylanases from Bacillus sp. Nl37 and B. stearothermophilus 21 with $61\%$ and $59\%$ identical residues, respectively.

• Journal of Sericultural and Entomological Science
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• v.34 no.2
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• pp.20-25
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• 1992

The location of the polyhedrin gene of Bmbyx mori nuclear polyhedrosis virus(BmNPV) was determined by using a cloned polyhedrin gene from the Autographa californica nuclear polyhedrosis virus(AcNPV) as a hybridization probe. The 7.4 Kb PstⅠ fragment DNA of Bm-NPV was cloned to plasmid pUC19 vector. A fragment containing this gene was mapped and sequenced in its entire polyhedrin reading frame. Nucleotide sequences comparison of the polyhedrin of the BmNPV to that of previously reported by Ⅰatrou(1985) revealed that the sequence varied in 10 base, Comparison of the amino acid sequence of the two structured gene revealed that coding sequence varied 74 valine to isoleucine, 76 aspargine to serine and 155 methionine to valine.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.1
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• pp.52-58
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• 1982

The effects of casein diet supplemented with excessive levels of three basic amino acidslysine, histidine and arginine - were studied in male, weanling, I. C. R. strain mice. The relationship between weight gain and concentration of blood urea nitrogen was also discussed with the follows results : 1. The groups supplemented with basic amino acids were demonstrated depressed growth and food intake than the control ($10\%$ casein diet) group, $5\%$ L-histidine HCl supplemented group being most depressed. 2. The concentration of blood urea nitrogen in the basic amino acid supplemented groups were higher than the control group. The value of L-arginine supplemented group was highest. 3. The concentration of blood urea nitrogen was related to the amounts of nitrogen contained in the diet not related to the growth gain.

• Journal of Animal Science and Technology
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• v.50 no.1
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• pp.9-18
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• 2008

Species-specific polymorphisms in chicken, pheasant, turkey, and quail were identified by cloning and sequencing of the immunoglobulin constant domain (IgLC). A set of species-specific primers were then designed on the basis of polymorphisms in the IgLC between species, as well as two additional sets of primers for the cytochrome b and tapasin genes, for the purpose of species identification. Together, the primers successfully distinguished specific species from chicken by species-specific PCR. This simple but unambiguous method may be used to screen avian inter-species germline chimeras, which are valuable models for the conservation of endangered species.

• Journal of the Korea Society of Computer and Information
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• v.17 no.11
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• pp.141-148
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• 2012

The advance of technologies for human genome makes it possible that the analysis of association between genetic variants and diseases and the application of the results to predict risk or susceptibility to them. Many of those studies carried out in case-control study. For quantitative traits, statistical analysis methods are applied to find single nucleotide polymorphisms (SNP) relevant to the diseases and consider them one by one. In this study, we presented methods to select informative single nucleotide polymorphisms and predict risk for quantitative traits and compared their performance. We adopted two SNP selection methods: one considering single SNP only and the other of all possible pairs of SNPs.

• Journal of the Korean earth science society
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• v.27 no.5
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• pp.528-538
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• 2006

Main aspect of this study is to clarify the petrochemistry and petrogenesis of intermediated-basic plutons located in the southwestern part of the Korean peninsula. These Intermediated-basic plutons consist of Pre-Cambrian anorthosite-gabbro, Triassic hornblende gabbro (Jirisan area), Jurassic diorite-syente (Jirisan and north area) and Cretaceous gabbro-diorite (south area). The Massif type anorthosite has multi intrusions, where each one intruded by gabbroic rocks, composed of gabbro, norite, troctolite and leucogabbro. In the variation diagram of the major-minor composition, AMF and Pl-Px-Ol diagrams, we suggest that intermediated-basic plutons in the southwestern part of the Korea show a trend consistent to Daly's value and calc-alkaline rock series. Accoding to REE (La/Yb)cw and Eu/Sm, these plutons are enriched with LREE than HREE, and emplaced by the tectonic setting in continent and/or continental margin.