• Journal of Environmental Science International
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• v.32 no.4
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• pp.221-232
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• 2023

This study investigated the antioxidant effect of Hermetia illucens larvae using different extraction temperatures and solvents. We found significant differences in total phenolic content (TPC), total flavonoid content (TFC), an in three antioxidant indexes 2,2-diphenyl-1-picrylhydrazyl (DPPH), 1,1'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and ferric-reducing antioxidant power (FRAP) contents, among the samples depending on the extraction temperature and solvent used. The sample extracted with water at 45℃ (HIW-45℃) showed the highest TPC, DPPH, ABTS, and FRAP contents, while the sample extracted with water at 90℃ (HIW-90℃) showed the highest TFC. These differences can be due to the different chemical structures of the extracted components. Based on these results, HIW-45℃ was the optimal extraction method for Hermetia illucens. We intend to further investigate the availability of functional materials for Hermetia illucens using this method.

• Journal of Environmental Science International
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• v.32 no.12
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• pp.965-972
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• 2023

As the world population growth and economy develops, the importance of eco-friendly organic waste resource treatment and up-cycling increases. Black soldier fly (BSF) (Hermetia illucens) treats organic waste resources that allows it to be recycled as a feed resource. In this study, we analyzed the nutrients composition, amino acid, and fatty acids of BSF larvae fed three agricultural by-products, namely apple pomace, mandarin waste, and oriental melon waste (mainly generated in South Korea). The highest BSF larvae crude protein and amino acid content was obtained in those fed mandarin waste compared with the other two diets (apple pomace and oriental melon waste). BSF larvae fed apple pomace had the highest crude fat, fatty acid, and lauric acid content compared with the other BSF larvae. Furthermore, all crude protein and fat content of BSF larvae fed the three agricultural by-products exceed that of the original agricultural by-products.

• Journal of Life Science
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• v.32 no.12
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• pp.997-1004
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• 2022

To verify the nutritional superiority and safety of Protaetia brevitarsis larvae fed a food source containing soybean curd cake, the nutrients and harmful substances of larvae reared only with fermented oak sawdust and a food source containing soybean curd cake were comparatively analyzed. The crude protein content was 50.0% in the larvae fed with a food source containing soybean curd cake (SCC), which was 1.1 times higher than that of the larvae fed with fermented oak sawdust (FOS) at 44.7%. Potassium, which showed the highest content among macrominerals, was 2.5 times higher in SCC (3,169.6 mg/100 g) than in FOS (1,245.9 mg/100 g). Among trace minerals, zinc showed 12.6 times higher content than FOS (8.2 mg/100 g) in SCC (103.0 mg/100 g). As a result of the analysis of harmful substances, all heavy metals met the edible insect control standards, and pathogenic microorganisms, such as E. coli and Salmonella spp, were not detected. According to the above study, the larvae of P. brevitarsis fed soybean curd cake contain various nutrients, and safety has also been confirmed, so it is judged to be suitable for use as food.

• Journal of Sericultural and Entomological Science
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• v.21 no.2
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• pp.23-26
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• 1979

우리 나라의 잠사업은 1960연대 경제개발초기단계에서 공업화와 농업발전을 위하여 큰 몫을 담당하였다. 총수출액이 겨우 1억불에 달했던 1964년 생사수출이 6.1%를 점하였고 수출액이 10억불에 달한 1971년에도 생사 및 견직물 수출이 7.4%를 차지함으로서 외화소득에 큰 공헌을 하였다. 양잠과 제사는 노동집약적인 산업이므로 잠재실업자가 많았든 당시에는 농촌지역의 고용창출과 소득향상에 뚜렷한 성과를 걷우었다. 또 양잠은 전국 어디에서나 가능하며 특히 산간오지에도 주지한 정부지원시책으로 많이 보급하여 중요한 현금소득원이 됨으로서 소득분배의 균형화에도 크게 공헌하였다. (중략)

• Journal of Life Science
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• v.31 no.12
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• pp.1094-1099
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• 2021

Hermetia illucens (Black soldier fly) is attracting attention as an environmental purification insect because it can supply a wide range of by-products of the agricultural food industry. Also, it has a potential feed for fish, birds, and pets due to a short life cycle and excellent nutritional components. Several pharmacological effects, including antimicrobial, of H. illucens have been reported. However, no study has focused on antiobesity effects of ethanol extract of H. illucens. In this study, we aimed to assess the anti-obesity effects of ethanol extract of H. illucens larvae (HIE) through inhibition of differentiation of 3T3-L1 preadipocytes into adipocytes. The amount of lipid accumulated in adipocytes was measured by oil red-O staining, and the inhibitory effect on adipogenesis was confirmed. The expression levels of factors related to adipocyte differentiation and fat synthesis were determined using Western blot analysis. Lipid droplet formation in adipocytes was remarkably inhibited by HIE. In addition, treatment with 400 ㎍/ml of HIE significantly reduced the expression levels of peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α-transcription factors involved in adipocyte differentiation. Therefore, the results of this study indicate that HIE is a potential anti-obesity agent because it inhibits adipocyte differentiation.

• Journal of Environmental Science International
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• v.32 no.11
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• pp.833-840
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• 2023

Insect-healing program is designed for farm use, and this study attempted to scientifically investigate its healing effect based on application methods. The insects used in the program are Trypoxylus dichotomus, Papilio xuthus, and Bombyx mori. Insect-healing program was conducted on 61 adults of over 50 years old and at two different farms for 12 weeks. Stress, anxiety, insomnia, and cognitive function were measured through pre- and post-surveys. Result of the analysis showed that cognitive function changed positively in direct experienced group (those that encountered live insects) and indirect experience group (those that encountered insects only through photos and videos). However, psychological variables such as stress and anxiety decreased and sleep time increased significantly only in the direct experienced group. These results demonstrate that an agro-healing program that involve the use of insect must include activities such as raising and taking care of live insect to achieve positive psychological healing effects.

• Korean journal of applied entomology
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• v.61 no.1
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• pp.221-227
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• 2022

In accordance with Article 6 of the 「Insect Industry Promotion and Support Act」, the National Institute of Agricultural Sciences conducted a fact-finding investigation in the insect industry for related workers in 2020. Most commonly, insect industry workers were involved in production (98.5%), the male ratio was high (80.4%), while those in their 50s comprised 36.4% of the workers. As for the uses of industrial insects, 66.5% was for food and medicine, 12.7% for feed, and 20.7% as pets. The most commonly produced industrial insect species by use were the white-spotted flower chafer (69.6%), Protaetia brevitarsis seulensis (Kolbe), for food and medicine; crickets (31.2%) for feed; and the rhinoceros beetle (45.6%), Allomyrina dichotoma (Linnaeus), as pets. Powder was the most common product form at 68.5%, and live insects constituted 40.9%. Insect farms with an annual income accounted for 77.3%, of which 65.4% had annual sales of less than 20 million won. Market acquisition was rated by most insect industry workers (84.0%) as the primary challenge. In conclusion, although the number of people employed in the insect industry is increasing annually, the number of young insect farmers (20s to 40s) is decreasing. The production of industrial insects was limited to a few species, and more than half of insect farms have very low incomes. Furthermore, insect farms have the greatest difficulty in market acquisition, necessitating the development of utilization technology to promote the consumption of industrial insects.

• Journal of Sericultural and Entomological Science
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• v.35 no.2
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• pp.145-148
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• 1993

생산성 저해 요인의 제거는 바로 생산성 향상으로 연결된다. 뽕밭의 생산성을 저해하는 요인은 여러가지지만 그 중에서 토양의 이화학적 불량과 농약 공해가 비중이 가장 크다고 볼 수 있다. 우리나라 뽕밭은 입지가 경사지에 많으므로 토심이 얕은데 비해, 뽕나무는 영년성 심근성 작물이므로 식재후 근권의 물리성에 따라 생산성이 좌우되는 비중이 매우 크다. 그러나 식재후 토양 물리성 개량은 화학성 개량보다도 어려우므로 그 결과 생산성이 낮은 상태를 벗어날 수 없다. 따라서 정기적인 심경이나 심토 파쇄를 통한 물리성 개량이 요망된다. 계속적인 금비위주의 비배관리에 의해 강산성 반응을 보이는 토양이 대부분이므로 석회에 의한 산성개량이 필요하다. 질소질의 과다시비는 수량을 감소시킬 뿐만 아니라, 엽질을 악화시키고 질산태 질소가 지하로 흘러 들어가 지하수의 오염을 일으켜 청색증과 암을 일으키는 원인이 될 수 있다. 심각한 정도로 축적된 인산과 칼리도 수량을 떨어뜨리고 환경을 해치는 원인이 되므로, 축적 양분의 재활용에 대한 연구가 계속되어져야 한다. 특히 사용의 편리성 때문에 고농도의 복합비료 위주의 비배관리가 반복되고 있는 실정이므로 토양검정을 받아서 단비를 이용하여 적정한 시비를 해야 할 것이다. 이러한 방법은 생산성의 향상, 영농비의 절감, 공해의 유발을 억제하는 여러가지 효과가 있다. 농약과 산업공해로부터 양잠의 보호를 위해서는 안전지대에 잠업의 단지화, 인근 농과와의 대화를 통해서 양잠에 안전한 농약을 사용토록 하는 한편, 연구분야에서는 심도 있는 연구를 통해 양잠농가를 보호할 수 있는 대책의 수립이 요망되고 있다. 종합해서 말하자면 연구기관의 심도있는 연구가 더욱 활발히 이루워져야 할 것이며, 농민은 개발된 새기술에 보다 깊은 관심을 갖고 활용하는 한편, 필요로 하는 기술을 연구기관에 개발을 요청할 정도의 적극적인 자세가 요구된다. 또한 연구기관의 농민의 중간 교량역할을 하는 지도부문에서도 보다 적극적인 활동이 요청된다.

• Journal of Life Science
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• v.31 no.10
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• pp.898-904
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• 2021

Locusta migratoria is a widespread locust species in many parts of the world and is considered an alternative source for the production of protein for value-added ingredients. We previously identified putative antimicrobial peptides derived from L. migratoria through an in silico analysis of its transcriptome. However, its anti-inflammatory effect has not been studied. In this study, we investigated the anti-inflammatory activities of the antimicrobial peptide locustacin (KTHILSFFPSFLPLFLKK-NH₂) derived from L. migratoria on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Locustacin (50, 100, and 200 ㎍/ml) significantly reduced the production of nitric oxide (NO) in LPS-stimulated macrophages without any cytotoxicity. Locustacin also inhibited the mRNA and protein expression of pro-inflammatory mediators, such as inducible NO synthase and cyclooxygenase-2, in contrast to the presence of LPS alone. Locustacin decreased the release of LPS-induced pro-inflammatory cytokines, including interleukin (IL)-6 and IL-1β, and their gene expression in a dose-dependent manner. Furthermore, locustacin (100 and/or 200 ㎍/ml) inhibited phosphorylation levels of extracellular signal regulated kinase, p38, and c-Jun N-terminal kinase. Locustacin also suppressed the degradation of inhibitory kappa B alpha, which was considered to be an inhibitor of nuclear factor kappa B (NF-κB). Collectively, these results demonstrate that locustacin can exert anti-inflammatory effects through the inhibition of mitogen-activated protein kinase (MAPK) phosphorylation, activation of NF-κB, and downstream inflammatory mediators in LPS-stimulated macrophage cells.

• Journal of Life Science
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• v.31 no.11
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• pp.987-994
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• 2021

Our previous studies have reported that antimicrobial peptides (AMPs) derived from the larvae of white-spotted flower chafer (Protaetia brevitarsis seulensis) exert anti-inflammatory and neuroprotective activities. This study explored the anti-inflammatory effects of protaetiamycine 9 (CVLKKAYFLTNLKLRG-NH₂), a novel AMP, derived from P. b. seulensis against lipopolysaccharide (LPS)-mediated inflammatory response in RAW264.7 macrophage cells. Protaetiamycine 9 (25, 50, 75, and 100 ㎍/ml) did not cause cytotoxic effects against RAW264.7 cells. The RAW264.7 cells were pre-treated with various concentrations of protaetiamycine 9 (25-100 ㎍/ml) for 1 hr and then exposed to LPS (100 ng/ml) for 24 hr. Protaetiamycine 9 treatments decreased the LPS-induced secretion of inflammatory mediators, such as nitric oxide (NO), in a dose-dependent manner. Protaetiamycine 9 (25-100 ㎍/ml) effectively downregulated the LPS-induced increase in mRNA and the protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), which are involved in the production of inflammatory mediators. Protaetiamycine 9 also suppressed the production and gene expression of pro-inflammatory cytokines, including interleukin (IL)-6 and IL-1β, compared to the presence of LPS alone. Furthermore, protaetiamycine 9 inhibited the degradation of inhibitory kappa B alpha (IκB-α) and the phosphorylation of mitogen-activated protein kinases (MAPKs), such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. In conclusion, these results suggest that protaetiamycine 9 exhibits LPS-mediated inflammatory responses by blocking IκB-α degradation and MAPK phosphorylation.