• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.91-91
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• 2020

국내 복분자는 서양에서 유래한 서양복분자(Rubus occidentalis)와 국내 자생종을 개량한 토종복분자(Rubus coreauns)가 혼용되고 있으며, 전통 한방약재로서 미숙과를 중심으로 국내에서 유통되고 있는 복분자의 영양성분적인 특성과 주요성분인 ellagic acid 함량을 비교하였다. 토종복분자는 광양에서 재배되고 있는 복분자를 수집하였으며, 외래종 서양복분자는 고창에서 재배되고 있는 복분자를 수집하여 사용하였다. 나머지는 국내에서 유통되고 있는 국내산과 중국산 복분자를 경동약령시장과 금산약령시장으로부터 구매하여 사용하였다. 영양성분으로는 조지방, 조단백질 그리고 탄수화물 함량을 측정하였으며, 유리당, 지방산, 유리 아미노산 17종의 함량을 측정하였다. 조지방의 경우 토종복분자는 1.90 %, 서양복분자는 3.03 % 이였으며, 중국산 유통품은 2.28 %, 기타 국내산 유통품의 경우 2.89 %으로 중국산복분자의 조지방 함량이 낮은 것을 확인하였다. 그러나 탄수화물 함량의 경우 70.28~71.85 %로 복분자간의 함량에 큰 차이를 발견할 수 없었다. 유리당의 경우에는 토종복분자의 경우 glucose가 19.03 mg/g, fructose 16.29 mg/g이 측정되었고 고창 서양복분자의 경우 glucose가 16.29 mg/g, fructose 12.76 mg/g이 측정되어 유리당의 총 함량은 차이가 없는 것으로 확인되었으나 토종복분자의 경우 glucose의 함량이 조금 높은 것을 확인하였다. 복분자의 지방산 조성을 비교한 결과 고창 서양복분자에서 불포화지방산의 함량이 19.49 mg/g 으로 광양 토종복분자의 7.69 mg/g에 비하여 월등히 높은 것을 확인되었으며, 불포화지방산 중 linoleic acid (12.19 mg/g), oleic acid (1.88 mg/g)와 linolenic acid (5.43 mg/g) 함량이 높았다. 복분자의 아미노산의 함량은 광양 토종복분자의 경우 4.50 mg/g, 고창 서양복분자의 경우 5.05 mg/g으로 유리아미노산의 함량은 유사한 것으로 나타났다. 특히 아미노산 17종 성분 중 asparagine(0.65~0.84 mg/g), arginine(0.51~1.00 mg/g)과 threonine(0.99~1.63 mg/g)의 함량이 높았다. 지표성분으로 ellagic acid의 함량은 광양 토종복분자의 경우 2.56 mg/g, 고창의 서양복분자의 경우 3.16 mg/g으로 측정되어 서양복분자가 조금 높은 것으로 나타났다. 국내 유통되고 있는 중국산 복분자의 ellagic acid의 경우 2.99 mg/g, 기타 국내산 유통 복분자의 경우 2.83 mg/g으로 광양 토종복분자와 유사한 것으로 나타났다. 위의 연구결과는 국내에서 유통되는 토종 및 서양 복분자를 원료로 하는 기능성식품에 대한 제품개발의 기초자료로서 활용 될 수 있을 것으로 기대한다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.98-98
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• 2020

본 연구의 목적은 약재로 사용되는 천궁 중 토천궁(Ligusricum chuanxing Hort.)에 대한 지역별 생육환경을 확인하고 이에 따른 지상부 및 지하부 생장과의 상관관계를 규명하여 토천궁의 적정 재배지역 확인 및 지속 가능한 안정적인 한약재 생산에 대한 기초자료로 사용함에 있다. 토천궁이 주로 재배되고 있는 봉화군, 영양군, 평창군과 더불어 기후변화에 의한 재배지 이동을 고려하여 고위도 지역인 인제군을 포함한 4지역의 재배지를 선정하였으며, 생육특성을 비롯 기상 및 토양환경을 분석하여 토천궁의 생육특성과의 상관관계를 분석하였다. 4지역의 재배지 중 지상부와 지하부의 전체적인 생육은 평창지역에서 유의적으로 높게 확인되었다. 그리고 생육특성과 기상환경과의 상관관계에서 대기 및 토양온도가 지상부의 전체높이, 줄기직경, 지하부의 생육특성과 P < 0.01수준에서 유의적인 음의 상관관계, 잎의 길이 및 너비와는 양의 상관관계를 보였다. 또한 일사량이 높아질수록 잎의 크기를 제외한 생육특성이 양의 상관관계를 나타냈는데 이는 대기 및 온도의 상승으로 인한 잎의 증발산율 상승과 일사량에 따른 잎의 광합성량의 차이에 의한 결과라 사료된다. 또한 토양특성과의 상관관계에서 OM과 N, P, K는 지상부의 전체 높이와 줄기 직경을 비롯한 지하부의 생육특성과 음의 상관관계를 보였는데 근권의 토양시료를 사용하여 분석한 것이라 실질적으로 토천궁의 생육에 필요한 토양의 성분이 OM과 N, P, K이며, 토천궁에 의해 흡수되었다고 판단되는데 생육이 가장 우수했던 평창지역 토양 내 OM(1.69%)과 N(0.09%), P(897.72mg/kg), K(0.21cmol⁺/kg)의 수치가 타 지역 대비 상대적으로 낮게 측정된 결과가 본 실험에서 확인된 생육에 대한 OM, N, P, K의 함량과 부의 상관관계를 지지하며, 본 연구의 결과는 향후 토천궁의 재배지 선정 및 재배기술 정립에 활용할수 있을 것이라 사료된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.69-69
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• 2020

소엽(Perilla frutescens var. acuta)은 흔히 차조기, 자소엽 등으로 불리며, 식재료나 약재로 이용된다. 최근 들어 자소엽을 향장 원료로 활용하고자 하는 시도가 이루어지고 있다. 따라서 유기자재 처리를 통해 향장원료 자소엽의 고품질 생산을 위하여 본 시험을 실시하였다. 전북 농업기술원 허브산채시험장에서 육묘한 종묘를 2020년 5월 노지 포장에 주간 간격 50cm 한줄 재배로 정식하였다. 처리는 규산염(성분량 20%), 키토산(성분량 5%)가 함유된 자재를 각각 500배, 1,000배 희석하여 6월 21일, 8월 3일, 8월 23일 총 3회에 걸쳐 압축 분무기를 이용해 작물이 충분히 적셔지도록 살포하였으며, 무처리구는 물을 살포하였다. 초장, 초폭, SPAD, 생체중, 건물중을 조사하였으며 잎과 꽃의 총 폴리페놀 함량, 총 플라보노이드 함량, FRAP(철환원 항산화력) 활성 ABTS+ 라디칼 저해활성을 측정하였다. 생육특성 중 초장과 초폭은 처리간 유의성은 없었으나 무처리, 규산염 500배, 키토산 1,000배 처리에서 대체로 높았으며, SPAD는 키토산 1,000배가 가장 양호하였다. 수량성은 모든 자재 처리구가 무처리구보다 많았으며, 지상부 생체중은 무처리 대비 키토산 1,000배는 14.1%, 건물중은 무처리 대비 11.9%로 키토산 1,000배 처리가 수량성이 가장 많았다. 총 폴리페놀 함량은 꽃보다 잎에서 더 높은 수치로 나타났으며, 잎의 폴리페놀 함량은 규산염 500배 처리에서 가장 많았다. 꽃은 1,000배 처리에서 가장 높았다. 총 플라보노이드 함량은 꽃이 잎보다 높았으며, 잎은 규산염 500배 처리, 꽃은 키토산 1,000배 처리에서 함량이 가장 많았다. FRAP 활성은 잎의 경우 규산염 500배 처리에서 높았으며, 꽃의 FRAP 활성은 키토산 1,000배에서 좋았으나 처리 간의 차이는 크지 않았다. 자소엽 잎의 ABTS+ 라디칼 저해 활성은 규산염 500배 처리에서 가장 높았으며, 꽃은 경우 무처리에 비하여 자재 처리가 높았으며, 500배보다 1,000배 처리에서 높은 라디칼 저해 활성을 보였다. 이상의 결과, 자소엽 유기자재 처리는 무처리보다 대체로 수량성과 항산화 활성이 높았으나 추가적으로 적정농도 구명 후 경제성 분석이 필요할 것으로 판단된다.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.1007-1016
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• 2023

In this study, the effect of functional substances such as polyphenols and flavonoids contained in large quantities in red ginseng and herbal medicines on the antioxidant activity properties and flavor effect were investigated by increasing the content and activity of functional ingredients by convert red ginseng through a steaming heat-drying process compared to the traditional dry processing method of ginseng and herbal medicines. According to the experimental results, the addition of pre-heat treatment significantly increased antioxidant properties such as DPPH radical scavenging ability, polyphenols, and flavonoids. In addition, during the steaming and heat-dried red ginseng manufacturing process, the amino-carbonyl browning reaction was promoted, resulting in increased brownness and a savory flavor. However, the content of ginsenosides, the main medicinal ingredient in red ginseng, became the main cause of the bitter and harsh taste. In addition, the bitter and harsh taste of red ginseng has been significantly improved by roasting and producing powder, but on the other hand, Radix Angelicae sinensis, polymorphic angelica and Peony, which are used as main medicinal ingredients in oriental medicine for nutritional tonic prescriptions such as 10 herbal medicine and 4 herbal medicine have a very strong herbal medicine-specific flavor and have a bitter and harsh taste. It is so strong that in order to use it as an instant extraction material, it was reviewed that a steaming and heating manufacturing method was needed during the manufacturing process.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.50 no.2
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• pp.163-170
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• 2024

In the literature, inhalation of cosmetic fragrances, including natural extracts or natural essential oils, has been reported to induce brainwave changes. Angelica gigas (AG) and Cnidium officinale (CO) are widely used in East Asian countries as traditional medicines to improve health, but there have been no previous reports of inhaling their aromas and assessing changes in brain activity through electroencephalogram (EEG) recordings. In this study, the scent of natural herbal extracts such as Artemisia princeps var. orientalis (Compositae), AG, and CO was inhaled and evaluated whether emotional state changes were caused by olfactory stimuli, and brain activity was confirmed through EEG records before and after inhaling the scent of natural herbal extracts in subjects aged 20 to 30. We also used an electrocardiogram (ECG) to record the electrical activity of the heart during fragrance administration. The test results indicated that inhaling the scents of Compositae, AG, and CO extracts, as well as the positive control lavender, significantly increased the relative alpha power and the alpha/beta ratio of brain waves across the brain regions. The ECG recordings indicated that the inhalation of Compositae fragrance significantly decreased the low-frequency/high-frequency ratio, and that the inhalation of traditional herbal extract fragrances resulted in comfort and relaxation.

• The Journal of the Convergence on Culture Technology
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• v.10 no.2
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• pp.555-563
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• 2024

We were selected oriental medicinal herbs known to be effective in strengthening muscles, improving sarcopenia, treating arthritis and osteoporosis. Amino acids have been reported to be associated with the above-mentioned effects. During the manufacturing process of water extracts of ginseng and samchae, the content of arginine, a basic amino acid, was significantly reduced. The decrease was 99.48mg/100ml for steamed red ginseng compared to 114.07mg/100ml for dried white ginseng, and 54.69mg/100ml for steamed samchae compared to 118.90mg/100ml for dried samchae. The extracts of the wild roots of Acanthus apias and the above-ground twigs of Acanthus apias showed significantly higher DPPH and ABTS radical scavenging activities. Meanwhile, DPPH activity was higher in steamed red ginseng and steamed samchae compared to dried white ginseng and dried samche, but ABTS activity increased to 66.88% for steamed red ginseng compared to 41.96% for dried white ginseng, but was 77.48% for dried samchae and 77.52% for steamed samchae.

• KSBB Journal
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• v.23 no.4
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• pp.329-334
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• 2008

The antioxidant activity and the qualitative analysis of Acanthopanax sessiliflorum Seeman were studied by partially purified extract using various methods: extraction by using ethanol solutions and temperatures, and absorption to Diaion HP20 column chromatography using 10%, 20%, 40%, 60% ethanol solutions. Major constituents, chlorogenic acid, caffeic acid, eleutheroside E, was determinated by HPLC method in Acanthopanax sessiliflorum S. 10% and 20% ethanol solutions contain chlorogenic acid (3.020$\pm$0.080%, 20.500$\pm$1.150%, respectively). 40% ethanol solution contains caffeic acid and eleutheroside E (12.270$\pm$0.360%, 1.670$\pm$0.140%, respectively). Diaion HP20 fractions (10%, 20%, 40%, 60% ethanol solutions) showed the scavenging activity of radicals and reactive oxygen species with the $IC_{50}$ values of $81.534{\pm}0.992{\mu}g/ml$, $1.748{\pm}0.098{\mu}g/ml$, $11.487{\pm}1.768{\mu}g/ml$, $21.960{\pm}0.547{\mu}g/ml$ against 1,1-diphenyl-2-picrylhydrazly radical and $1713.548{\pm}34.565{\mu}g/ml$, $131.419{\pm}2.235{\mu}g/ml$, $200.681{\pm}2.444{\mu}g/ml$, $757.897{\pm}6.868{\mu}g/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. Especially, 20% and 40% ethanol fractions showed more antioxidant activity than dl-$\alpha$-tocopherol. These results suggest that Acanthopanax sessiliflorum S. extract and Diaion HP20 fractions may be useful as a potential source of nutraceutical and cosmetic products.

• The Journal of Korean Obstetrics and Gynecology
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• v.30 no.2
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• pp.49-70
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• 2017

Objectives: The present study is to observe the skin-regeneration, anti-wrinkle, whitening and skin moisturizing effects of Cheongsangbangpung-tang (CSBPT) with cytotoxicity. Methods: In the present study, cytotoxicity of CSBPT lyophilized aqueous extracts (yield=18.71%) was experimented against human normal fibroblast cells and B16F10 murine melanoma cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay, and skin regeneration and anti-wrinkle effects were also showed through the assay of collagen type I synthesis by an enzyme immunoassay (EIA) kit as comparing with transforming growth factor (TGF)-${\beta}1$, hyaluronidase, collagenase and matrix metalloproteinase (MMP)-1 inhibitory assays as comparing with oleanolic acid (OA), and elastase inhibitory effects as comparing with phosphoramidon disodium salt (PP). In addition, whitening effects of CSBPT were observed by tyrosinase inhibitory assay and melanin formation test in B16/F10 melanoma cells as comparing with arbutin, and skin moisturizing effects were measured through mouse skin water contents test, respectively. Results: No CSBPT treatment related cytotoxic effects were demonstrated against human normal fibroblast cells and B16/F10 murine melanoma cells. CSBPT concentration-dependent increased collagen type I synthesis at human normal fibroblast cells. It also effectively suspreessed hyaluronidase, collagenase, elastase and MMP-1 activities, which were enzymes that related to declining of ECM and formation of wrinkle. CSBPT supressed B16/F10 melanoma cells's melanin productions with tyrosinase activity, which was an enzyme connected with melanin formation, and dose-dependent and significant increases of skin water contents were detected in CSBPT treated mouse skin as compared with vehicle control skins. Conclusions: CSBPT showed favorable and enough skin regeneration, anti-wrinkle, whitening and skin moisturizing effects at least in a condition of this experiment. However, more detail mechanism and in vivo skin protective efficacy studies should be conducted in future with the screening of the biological active compounds in individual herbs of Cheongsangbangpung-tang.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.4
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• pp.329-336
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• 2016

Cutaneous microvasculature plays a critical role in age-associated skin changes. A considerable reduction of number and size of vessels has been observed in the upper dermis of elderly skin. Forsythiae fructus (FF), the dried fruit of plant Forsythia suspensa (F. suspensa), has been traditionally used as an herbal medicine to treat inflammatory diseases and bacterial diseases. However, its regulatory effect on angiogenic responses has not been elucidated in skin. Therefore, we analyzed secretory profiles upon treatment of FF extract using array designed to detect angiogenesis-associated mediators in human keratinocytes. Because keratinocyte-derived VEGF (vascular endothelial growth factor) has been regarded as a potent factor for new microvasculature under the epidermis, we further investigated the effect of FF extract on VEGF production. We observed that the VEGF expression of mRNA and protein level was increased by about 2 folds in a dose-dependent manner after FF extract treatment. In signaling experiments, FF extract induced rapid p38 MAPK activation within 5 min, and the activation was totally abrogated by pretreatment with a p38 MAPK specific inhibitor. The FF-induced VEGF upregulation was also significantly attenuated by a p38 MAPK inhibition. Taken together, FF extract induces VEGF production via p38 MAPK activation in human epidermal keratinocytes. These novel findings suggest that FF is useful as a potential agent with pro-angiogenic activity and may help to improve age-dependent reduction of the microvasculature in aged skin or to heal skin wound.

• Journal of Food Hygiene and Safety
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• v.22 no.3
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• pp.213-217
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• 2007

LPS induces the synthesis of several inflammatory cytokine, chemokine, NO and inflammation in the liver of rats. The purpose of this study was to investigate the preventive effects of Rubus coreanum Miquel (RCM) In lipid metabolism. RCM of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5ml/kg for 20 days. On the day 21, 1.5ml/kg of LPS was injected 4 hours before anesthetization. We examined the lipid-related functions by measuring the levels of triglyceride (TG), total cholesterol (TC), total lipid (TL), high-density lipoprotein cholesterol (HDL-C) in serum and malondialdehyde(MDA) in liver tissue. The results showed that LPS treatment increased the values of TG, TC, TL and MDA, decreasing that of HDL-C. But RCM pretreatment decreased the high values of TG, TC, TL and MDA to the low values and increased the low value of HDL-C to the high value. These results suggested that RCM could be used as the potential candidate for the lipid metabolism natural supplement.