• Title/Summary/Keyword: 암조건

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Fermentation Characteristics of Cheonggukjang made of Germinated Soybean under Light Condition (명조건에서 발아시킨 콩을 이용한 청국장의 발효 특성)

  • Kim, Mi-Hyang;Lee, Nan-Hee;Choi, Ung-Kyu
    • Journal of Life Science
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    • v.18 no.10
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    • pp.1420-1425
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    • 2008
  • This study was conducted to investigate the fermentation characteristics of cheonggukjang prepared with soybean germinated under light condition. The moisture content and pH of cheonggukjang made with soybean germinated under light condition (CGL) were higher than that of cheonggukjang made with non-germinated soybean (CNG). The color of CGL was darker than that of CNG and cheonggukjang made with soybean germinated under dark condition (CGD). The number of live bacteria in the CGL ($10.7{\pm}0.3\;\log\;CFU/g$) and CGD ($10.7{\pm}0.4\;\log\;CFU/g$) was higher than that of CNG ($9.7{\pm}0.4\;\log\;CFU/g$). The levels of isoflavone in CGL, CGD and CNG were 2,038.0, 1,993.6 and 1,747.0 ug/g, respectively. There were no significant differences in the appearance, aroma, taste and overall acceptability of the cheonggukjang made with different germination conditions. Therefore, it was expected that the cheonggukjang prepared with soybean germinated under light condition could be practically used as a new fermented food.

Carotenoid Production of Transformed Carrot Hairy Root and Its Culture in Bioreactor (형질전환된 당근 모상근의 Carotenoid 생산과 생물반응기에서의 배양)

  • 고경민
    • Journal of Plant Biology
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    • v.35 no.4
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    • pp.365-370
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    • 1992
  • 다양한 배양조건을 이용하여 당근 모상근의 생장과 carotenoid 함량을 조사하였으며, 이러한 배양조건을 airlift형생물 반응기에 적용하여 모상근의 다량 배양을 시도하였다. 고형배지 배양에서의 carotenoid 함량은 광조건에서 배양된 모상근이 암조건에서 배양된 모상근보다 10배 정도 증가하였으며, 모상근의 생장은 광조건이 암조건보다 낮은 수준이었다. 공기 유출기 배양에서는 모상근이 광조건과 암조건 모두에서 매우 빠르게 생장하여 생줄량의 차이가 없었으며, 모상근의 생장과 carotenoid 함량에 있어서 빛의 조사는 모상근의 전체적인 생중량을 증가시키기 보다는 배양 후기 생장단계에서 carotenoid 함량을 증가시켜서 암조건에 비하여 10배 정도의 carotenoid(11 $\mu\textrm{g}$/g)를 생산하였다. 모상근을 생물 반응기에서 배양하였을 때, 모상근이 지수함수적으로 빠르게 생장하여 배양 25일 동안에 처음 무게의 11배를 나타내었다. 그리고 pH는 처음 5.5에서 5일후 4.9로 떨어지다가 그 이후에 일정한 수준(pH 4.8)을 보여주었으며, 광주기와 광도을 조절하지 않은 상태에서의 carotenoid 함량은 2$\mu\textrm{g}$/g으로 나타났다.

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The Effects of Light and Dark Adaptation upon the Compound Eye of Nilaparvata lugens (벼멸구 겹눈의 명적응과 암적응 효과)

  • Young Nam Youn
    • Korean journal of applied entomology
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    • v.34 no.4
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    • pp.334-343
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    • 1995
  • The eyes of Nilaparvata lugens were examined for ultrastructural changes in the light and dark adapted states. Inspection of light microscope sections taken at similar levels of compound eyes from insects kept in light or darkness for periods up to 72 hors revealed some differences between light and dark adapted eyes. Using the electronmicroscope, in light adapted eyes the palisade layer was narrower than that in dark adapted eyes. The pigment granules still formed a ring around the palisade layer in the dark adapted eye but, they did not form a tight circle around the rhabdom. No constant difference was found between the diameters of the microvilli in light and dark adapted eyes. The pigment movements at the junction of the cone and the rhabdom took the effect on varying the pigment aperture at the tip of the cone in front of the rhabdom tip.

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Changes of Moisture Uptake. Respiration and Chemical Contents in Germinating Tobacco(Nicotiana tabacum L.) Seeds (담배종자의 발아조건에서 수분흡수, 호흡 및 저장물질의 변화)

  • 민태기;윤경은;김웅주;강정용
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.3
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    • pp.293-298
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    • 1986
  • The physiological aspects of tobacco seeds from seeding to germination under light and dark condition, and different temperature were studied to obtain basic information on the production of good seedlings. There were distinct three phases in moisture uptake and respiration process during germination that classifiable into 'Imbibition', 'Lag', and 'Growth' phase under light condition but such growth phase were not observed in the dark germination. Great changes of sugar and fatty acid content of tobacco seeds were observed during germination in light condition but such changes were slight in dark germinated seeds and fatty acid content of seeds during germination were decreased as corresponding to increasing in respiration.

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Degradation of herbicide paraquat by Fenton reagent and UV light irradiation (Fenton 시약 및 UV 광 조사에 의한 제초제 paraquat의 분해)

  • Kim, Byung-Ha;Ahn, Mi-Youn;Kim, Jang-Eok
    • The Korean Journal of Pesticide Science
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    • v.3 no.3
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    • pp.20-26
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    • 1999
  • This study was to investigate the potential degradation of a herbicide paraquat by Fenton reagents(ferric ion and hydrogen peroxide) under UV light irradiation(365 nm) in an aqueous solution. When $10{\sim}500$ mg/L of paraquat was reacted with either ferric ion or hydrogen peroxide in the dark or under UV light, no degradation was occurred. However, the simultaneous application of both ferric ion(0.8 mM) and hydrogen peroxide(0.140 M) in paraquat solution(500 mg/L) caused dramatic degradation of paraquat both in the dark (approximately 78%) and under UV light(approximately 90%). The reaction approached an equilibrium state in 10 hours. In the dark, when $0.2{\sim}0.8$ mM ferric ion was added, $20{\sim}70%$ paraquat of $10{\sim}500$ mg/L was degraded, regardless of hydrogen peroxide concentrations($0.035{\sim}0.140$ M), while under UV light, 95% of 10 and 100 mg/L paraquat was degraded regardless of ferric ion and hydrogen peroxide concentrations. At paraquat concentration of 200 and 500 mg/L, paraquat degradation increased with increasing ferric ion concentrations as in the dark. However the increase in hydrogen peroxide concentration did not affect the extent of paraquat degradation. The initial reaction rate constants(k) for paraquat degradation ranged from 0.0004 to 0.0314, and 0.0023 to 0.0367 in the dark and under UV light, respectively. The initial reaction rate constant increased in proportion to the increase in ferric ion concentration in both conditions. The half-lives of paraquat degradation(t1/2) were 20 - 1,980 and 19 - 303 minutes in the dark and under UV light, respectively. This study indicates that Fenton reagents under UV light irradiation are more potent than in the dark in terms of herbicide paraquat degradation in an aqueous solution.

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Effect of Light and Cadmium on the Activity and Isozyme Pattern of Catalase from Ric(Oryza sativa L.) (빛과 카드뮴이 벼 catalase 활성과 동위효소 발현에 미치는 영향)

  • Kim, Yoon-Kyoung;Lee, Mi-Young
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.287-292
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    • 2006
  • The effects of cadmium on the catalase activity and isozyme patterns under light and dark conditions of rice(Oryza sativa L. cv. Dongjin) seedlings were examined. Cadmium treatment resulted in the notable enhancement of $H_2O_2$ contents in the seedling roots and leaves under light and dark conditions. The catalase isozyme patterns in the roots were different from those in the leaves, showing tissue-specific expression of the enzyme. Moreover, the expression patterns of catalase isozymes in the green seedling roots were different from those in the etiolated seedling roots following cadmium treatment. The increase of total catalase activity was about 16 times at 1 mM cadmium and marked inductions of the isozyme CAT1 and CAT2 contributed to this increase in the green seedling roots. On the other hand, in the etiolated seedling roots, total catalase activity was lower than that of control at 0.5 and 1 mM cadmium, even though catalase activity increased about 3 times at 0.1 mM cadmium. The 3 fold increase of total catalase activity was mainly due to the increase of CAT1, CAT3 and CAT4 at 0.1 mM cadmium. However, treatment with higher concentrations of cadmium decreased the activity of CAT2 and CAT4 in the etiolated roots. In the leaves, the catalase existed as three isozymes; one cationic isozyme CATc, one neutral isozyme CATn and one anionic isozyme CAT1 in the control. The isozyme patterns and total activities remained unaffected by cadmium under light and dark conditions in the seedling leaves. Taken together, it seems that cadmium-induced changes of catalase might be regulated by light in the roots, but not in the leaves.

구강암환자의 치과치료

  • Kim, Yong-Gak
    • The Journal of the Korean dental association
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    • v.24 no.7 s.206
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    • pp.583-591
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    • 1986
  • 근년에 이르러 암환자의 증가, 암치료성적의 호전, 그리고 암환자의 통원치료 증가등으로 암전문변원이나 암연구소의 치과의사(institutional dentist)뿐만 아니라, 일반 치과의사(non-institutional dentist)들도 암과 관련된 환자들을 접하는 기회가 늘고 있다. 일반적으로 구강함환자는 구강암 자체, 혹 그 처치를 통하여 전신적으로, 혹은 국소적으로 위약된 조건을 갖게되므로 치과치료시에는 특별한 지식과 세심한 주의가 필요하다. 이러한 필요에 이해 선진국에서는 이미 치과종양학(Dental Oncology)의 임상이 활발하다. 이제 이러한 치과종양학적 입장에서 외과적 수술, 방사선요법, 그리고 화학요법을 통한 구강암 (혹은 두경부암)의 처치시에 치과의사의 역할과 치과치료의 문제점 및 그 해결책에 대하여 기술하고자 한다.

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Development of "Safety Footing for Passage for Moving Pylon Arms" to Prevent Falling of Pylon Boarders (철탑승탑자 추락예방을 위한 "철탑 암(Arm) 이동을 위한 통로용 안전발판" 개발)

  • Kwon, Soonho
    • KEPCO Journal on Electric Power and Energy
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    • v.7 no.2
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    • pp.233-236
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    • 2021
  • 종래의 사용중인 송전철탑 암(Arm) 이동 또는 작업을 위해서는 암주재를 발로 딛고 이동 하거나 작업하는데 발디딤 공간이 부족하여(7~15cm)안전사고 발생요인이 되고 있다. 이러한 문제점을 개선하고자 철탑 암 이동 또는 작업 시 작업자의 심리적 안정감과 물리적 안전조건을 강화시키기 위해 발디딤 공간을 30cm 로 확대시킨 "철탑 암 이동을 위한 통로용 안전발판"(이하, 철탑 암 안전발판)을 개발하였다.

Effects of Temperature, Light Condition, and Priming Treatment on Seed Germination of Bidens bipinnata L. (온도, 광 및 Priming 처리가 도깨비바늘 종자의 발아에 미치는 영향)

  • Lee, Sang Yong;Baek, Jun Pill
    • Journal of Bio-Environment Control
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    • v.22 no.2
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    • pp.188-191
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    • 2013
  • This study was carried out to investigate the effects of light, temperature, and priming treatment on the germination of Bidens bipinnata seeds for the purpose of cultivation of new vegetable crop. The results showed that the treatment of $25^{\circ}C$ yielded the highest germination percentages and the highest germination speed in both light and dark conditions. Light condition had no effect on the germination of B. bipinnata. Priming treatments demon-strated the following improved germination percentages: $Ca(NO_3)_2$ 0.1 M (68.3%), $K_3PO_4$ 0.1 M (63.3%), and $KNO_3$ 0.1 M (53.3%), with $Ca(NO_3)_2$ 0.1 M treatment giving the greatest improvement. Consequentially, the results suggested that $25^{\circ}C$ light condition, and $K_3PO_4$ 0.1 M or $Ca(NO_3)_2$ 0.1 M priming treatments would be effective method to improve the germination of Bidens bipinnata seeds.

Investigation of Several Conditions for Efficient Cultivation of Two Native Medicinal Plants (자생 약용식물 2종의 효율적인 재배를 위한 적정 조건 구명)

  • Soyeon Park;Kyungtae Park;Jihun Lee;Bo-Kook Jang;Cheol Hee Lee;Ju-Sung Cho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.43-43
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    • 2022
  • 우리나라의 많은 자생식물은 약용 및 관상용으로 이용되어 안정적인 공급을 위해서는 적절한 재배 조건에 대한 정보가 필요하다. 본 연구에 사용된 비수리[Lespedeza cuneata (Dum.Cours.) G.Don]와 제비쑥(Artemisia japonica Thunb.)은 각 야관문과 모호라는 이름으로 불리며 약용자원으로서 가치가 높은 식물이나 적정 재배조건에 대한 정보가 부족하다. 따라서 효율적인 재배 조건을 제시하기위해 재배 시 토양, 차광, 추비가 식물의 생육에 미치는 영향을 비교하였다. 실험에 사용된 식물의 종자는 2022년 국립생물자원관에서 분양 받았다. 두 종의 종자를 페트리디시에 파종한 후 15, 20, 25, 30℃(명 또는 암조건)과 25/15℃(16/8h, 명/암) 조건에 파종하여 발아 및 휴면 여부를 확인하였다. 재배 실험은 유리온실에서 원예용상토에 파종한 다음 비수리는 4주, 제비쑥은 6주 동안 생육시킨 균일한 묘를 각 지름 7, 9cm 포트에 이식하여 실험에 사용하였다. 토양실험은 원예상토와 마사토의 비율을 각 1:1, 2:1 및 3:1로 혼합하여 사용하였으며, 차광조건은 무차광, 55, 75%로 설정하였다. 추비 조건은 500 및 1,000ppm 하이포넥스 용액을 재배 6주 후 2주 간격으로 총 3회 엽면시비 하였으며, 원예상토 단용을 모든 실험의 대조구로 사용하였다. 발아실험결과, 비수리는 광조건의 모든 온도에서 발아하였으며, 암조건에서도 유사한 결과를 보였다. 반면 제비쑥은 20℃ 이하의 광조건에서 발아율이 유의적으로 감소하는 경향이었으며, 암조건에서는 모든 온도에서 발아율이 저조하였다. 재배조건 실험 결과, 두 식물 모두 원예상토와 마사토를 3:1로 혼합한 토양이 가장 생육에 적합하였으며, 1,000ppm의 하이포넥스로 추비 처리시 생육이 촉진되었다. 반면 모든 차광조건에서는 식물의 생육이 부진한 모습을 보여, 생육에 부정적인 영향을 주었다.

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