• 한국신재생에너지학회:학술대회논문집
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• 2009.06a
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• pp.443-446
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• 2009

바이오매스 에너지라 함은 생물체를 구성하는 유기물을 이용하는 에너지이다. 바이오매스는 에너지 위기 및 $CO_2$에 의한 지구온난화 및 화석자원의 고갈이 진행되면서, 화석연료와 달리 재생이 가능하고 지속 가능한 자원으로 각광을 받고 있다. 그 중에서도 목질계 바이오매스는 다른 신재생에너지원에 비해 국내 잠재량이 가장 풍부한 에너지원 중의 하나이다. 바이오매스 에너지 기술로는 직접연소, 열화학적 변환, 생화학적 변환의 기술이 있다. 본 연구에서는 목재를 원료로 한 부분산화 조건의 숯 생산 공정에서 목재의 열분해 가스 생산특성을 고찰하였다. 열분해가스 중에 응축된 목초액의 pH는 3.58～3.92 정도로 분석 되었고, 산도는 시간이 경과 할수록 2.74에서 4.44%로 농도가 증가 되었다. 숯 생산 공정에서의 목재의 열분해는 초기부터 48시간까지는 열분해가스의 조성의 변화가 거의 없었고, 48시간 경과 후에는 열분해가스 중에 가연성가스인 $H_2$, CO, $CH_4$가 약 5%정도 배출되는 것을 알 수 있었다.

• KSBB Journal
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• v.6 no.1
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• pp.63-68
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• 1991

An auxotrophic regulatory mutant of Corynebacterium glutamicum ATCC 21674 produced 2.1-3.4g/1 of phenylalanine with 2.9-4.4g/l of tyrosine in the batch shake flask fermentations. At higher sugar concentration, the production of both amino acids was lower than that at low sugar concentration. There was a pronounced effect of temperature on the amino acid production. At $30^{\circ}C$, much higher levels of phenylalanine and tyrosine were produced than those at $37^{\circ}C$. The pH decrease in the shake flask fermentation was so fast that it was impossible to maintain a constant pH with calcium carbonate as a buffering agent. Even though the strains we have used are reported as tyrosine auxotrophs, they produced tyrosine and were able to grow on the minimal medium where no tyrosine was present.

• KSBB Journal
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• v.23 no.1
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• pp.54-58
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• 2008

Hydrogen is considered as an energy source for the future due to its environmentally friendly use in fuel cells. A promising way is the biological production of hydrogen by fermentation. In this study, the optimization of medium conditions which maximize hydrogen production from Enterobacter aerogenes KCCM 40146 were determined. As a result, the maximum attainable cumulative volume of hydrogen was 431 $m{\ell}$ under the conditions of 0.5M potassium phosphate buffer, pH 6.5 medium containing 30 g/L glucose. The best nitrogen sources were peptone and tryptone for the cell growth as well as hydrogen production. The control of cell growth rate was found to be a important experimental parameter for effective hydrogen production

• Microbiology and Biotechnology Letters
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• v.15 no.2
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• pp.112-115
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• 1987

Optimal pH temperature and sucrose content for the production of vitamin B$_{12}$ and riboflavin by Bacillus megaterium and Enterobacter aerogenes was studied by microbiological analysis. Optimal pH for the production of B$_{12}$ was 6.0 by B. megaterium while the pH for E. aerogenes was 5.0. However, upon the addition of sucrose the optimal pH for B. megaterium shifted to 7.5 but E. aerogenes remained at pH 5.0. In the absence of sucrose, pH did not influence the yields of riboflavin produced by either bacterium. Addition of sucrose stimulated synthesis of riboflavin by both bacteria. Temperature had little effect on the production of vitamins by either bacterium.

• Journal of Life Science
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• v.9 no.3
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• pp.293-299
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• 1999

Accumulation of toxic waste byproducts in hybridoma culture can limit cell growth and monoclonal antibody (MAb) productivity, and one of the major toxic metabolites is lactate produced via glycolytic pathway of glucose metabolism. The factors affecting the glucose to lactate conversion rate were investigated. The conditions of high initial glucose concentration and high growth rate stimulated glucose to lactate conversion rate. The glucose-controlled fed-batch culture was investigated, and 19% reduction in lactate formation and 41% enhancement of MAb titer could be achieved by fed-batch culture.

• KSBB Journal
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• v.14 no.2
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• pp.205-211
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• 1999

We investigated optimal conditions for the microbial transformation of $\gamma$-butyrobetaine into L-carnitine by using Achromobacter cycloclast ATCC 21921. When the cells were cultivated in the medium containing $\gamma$-butyrobetaine as the sole carbon source for both cell growth and L-carnitine production, the maximum L-carnitine production was 2.9 g/L and the conversion yield from $\gamma$-butyrobetaine to L-carnitine was as low as 30.9 mol%. In order to enhance the L-carnitine production and the conversion yield, various carbon sources were added to the $\gamma$-butyronetaine containing basal medium. In the medium supplemented with glycerol, L-carnitine production was as high as 4.6 g/L and the conversion yield was 88.2 mol%, showing a significant improvement in L-carnitine synthesis compared to those in the medium without glycerol. We also examined the additional effect of quaternary ammonium compounds such as betaine and choline, which are similar in structure to $\gamma$-butyrobetaine and L-carnitien. It was observed that in the presence of those quaternary ammonium compounds, both the L-carnitine production rate and the conversion yield increased. In addition, we found that cell growth was inhibited by a $\gamma$-butyrobetaine concentration of more than 3%, while L-carnitine production was efficient at the $\gamma$-butyrobetaine concentration of 2-3%. By cultivating the cells in the optimal medium containing glycerol and choline, we obtained an L-carnitine concentration of 7.2 g/L with the conversion yield of 98.7 mol% in 4 days.

• KSBB Journal
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• v.14 no.4
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• pp.476-481
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• 1999

S. cerevisiae mutant(reg1-501, gal1), which cannot use galactose and has alleviated glucose repression level, is used as host for optimizing induction of GAL promoter. The optimum concentration of galactose as inducer for recombinant protein production and the galactose consumption rate have been tested with S. cerevisiae mutant and compared with conventional S. cerevisiae. The extent of glucose repression were investigated for both strain and the degradation pattern of produced foreign protein have been compared in both cases. The effect of pH on foreign protein degradation pattern were studied for both strains. The secetion efficiency of both strains were carried out. Through these experiments, optimum condition of recombinant protein production by GAL promoter using S. cerevisiae mutant (reg1-501, gal1) were found.

• KSBB Journal
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• v.15 no.5
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• pp.482-488
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• 2000

The Fab fraction of PDC-E2 specific human monoclonal antibody was produced using recombinant E. coli, and the effects of glucose and acetate were investigated to develop an optimal strategy for recombinant human antibody production. Higher glucose concentration in the culture media resulted inn higher cell growth and glucose consumption rate, which in turn resulted in an increased acetate production rate. When glucose was depleted, cells began to consume acetate as an energy source, and this consumption rate depended on the glucose concentration. When the residual glucose concentration was high, the accumulation of acetate was accelerated due to an increase in the acetate production rate and a decrease in the acetate consumption rate. Futhermore, it was found that a high accumulation of acetate, accompanied by a high glucose concentration, inhibited human antibody formation; the critical acetate concentration was $0.6g/\ell$. During production, a high glucose concentration enhanced cell growth, but inhibited antibody formation due to catabolic repression. Therefore, it is important to keep the concentration of both glucose and acetate as low as possible to increase antibody production after induction. Accordingly, it is important to accurately control the concentration of glucose and acetate in the culture media to obtain high cell densities and high productivity levels of recombinant human antibody.

• KOREAN POULTRY JOURNAL
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• v.38 no.11 s.445
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• pp.146-149
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• 2006

2006년도 9월에 조사된 가축통계에 따르면 국내 산란계 사육수수는 5,500만수로 국내 1인당 산란계 1마리 이상이 사육되고 있다. 하루 1개의 알이 생산되고, 국내 수출입되는 물량을 배제한다면 1인당 하루 1개의 계란을 섭취할 수 있는 양이다. 소비자들은 계란이 인류가 발견한 최고의 완전식품으로 50g의 고단백 영양덩어리라고 잘 알고있다. 하지만, 2003년 조류인플루엔자의 발생으로 당시 계란의 오염 가능성을 제기하면서 생산에 비해 소비가 떨어지는 추세가 나타나고 있으며, 언론을 통해 계란의 안전성 문제가 자주 제기되고 있는 실정이다. 이와 관련하여 본회에서는 계란에 대한 잘못된 오해를 풀고자 국방대학교 안보과정 교육생 일행을 초청해 조인(주)의 Grade Packing Center(등급포장센터)를 방문하여 농장에서 생산된 계란이 어떻게 위생적으로 포장납품되는지를 둘러보는 기회를 마련하였다.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1994.04a
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• pp.438-442
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• 1994

절삭가공에서 공구의 마멸은 생산의 최적화에 영향을 주는 가장 중요한 요소중의 하나라고할 수 있다. 따라서 생산시스템이 자동화되고 유연성 및 생산성이 증대되면서, 공구의 수명이 끝났을 때의 공구교환을 위한 최적 의사 결정전략(Decision making stratagy)은 그 중요성이 점차 커지고 있다. 한편, 공구는 마멸의 진행에 따라 그 수명을 예측하여 교환해 주는 것이 바람직하다. 그러나 공구의 마멸은 여러가지 요인들의 복합적 작용에의해 발생하는 현상 이므로 그것을 정확히 예측한다는 것은 많은 어려움이 있다. 본 연구에서는 절삭력을 이용하여 공구의 여유면마멸 (Flank wear)과 경사면마멸을 감지하고자 한다 먼저 절삭력을 정적인 성분(Static component)과 동적인 성분(Dynamic component)로 구분하여 공구의 마멸을 감지하는데 이용하였다. 절삭력의 정적인 성분은 절삭조건의 변화에 대해 정규 화된(Normalized) 절삭력으로 모델링하여 공구의 여유면 마멸을 감지 하였다. 또한 공구의 경사면마멸이 발생한 경우에는 절삭력의 동적인 성분이 크게 변화함을 알 수 있었다.