• Title/Summary/Keyword: 알코올류

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Characterization of polysaccharide A-1 from Opuntia ficus-indica and it's protection effect on alcoholic induced hepatic oxidative stress (Opuntia ficus-indica 다당 A-1의 특성 및 알코올유도 간 산화스트레스의 보호 효과)

  • Ryu, Il-Hwan;Kwon, Ji-Wung;Lee, Eoh-Jin;Yun, Young-Gab;Kwon, Tae-Oh
    • Herbal Formula Science
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    • v.17 no.2
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    • pp.163-174
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    • 2009
  • Reactive oxygen species(ROS) can induce hepatotoxicity and trigger apoptosis in the liver. In this study, we investigated the sulfated polysaccharide A-1 from Opuntia ficus-indica against alcoholic oxidative stress in human liver Hep G2 cell. An antioxidant substance A-1 obtained from the enzymatic extract of Opuntia ficus-indica fruit was purified by DEAE-cellulose ion exchange and sephadex G-100 gel permeation chromatography. The purification yield and molecular weight were 14.3% and 1.8 KDa, respectively. The A-1 predominately contained arabinose, galactose, rhamnose and also sulfate group. The structure of A-1 was investigated by periodate oxidation, FT-IR spectroscopy, $^1H$-NMR spectroscopy. The A-1 mainly composed of alternating unit of ${\rightarrow}4$)-$\alpha$-L- Rapp-2-$SO_3^-$-$\alpha$-L-Galp-($1{\rightarrow}$ and branched linkage of $\beta$-D-Arbp- ($5{\rightarrow}$. The antioxidative activity was measured using the SOD, CAT activity and GSH assay, respectively. The expression of Nrf2 protein was analyzed by western blotting. The viable cell count analyzed by autofluorescence. Oxidative stress induced by ethanol(1 M) were dramatically reduced by A-1 treatment. A-1 also prevented cell death induced by oxidative stress. It also increased expression Nrf2 protein level. We concluded that sulfated polysaccharide A-1 from Opuntia ficus-indica effectively protect Hep G2 liver cell from alcoholic oxidative stress.

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Color Index and Coloring Substances in Korean Sugar Maple, Acer mono Max.,Syrup (고로쇠나무(Acer mono Max.)수액(樹液) 표준농축액(標準濃縮液)의 색도지수(色度指數)와 착색물질(着色物質))

  • Ahn, Won Yung
    • Journal of Korean Society of Forest Science
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    • v.26 no.1
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    • pp.7-12
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    • 1975
  • The sap uses of Korean sugar maple, Acer mono Max., "Gorosoe-namoo," the most abundant growing stock in maples, for a medical beverage at every early spring have had the long history in Korea. The sap of Korean sugar maple (the maple) was prepared with a unique tapping method from the trees during the sap flow season of 1974, measured the sugar content in $2.6^{\circ}$ to $3.3^{\circ}Brix$ of sugar on average and the sugar concentration of the sap was enough for a sweetener resource comparing with those of American sugar maples in literature. The color of sirup was very dark and the color index was 2.69 for the sirup of $15.3^{\circ}Brix$ having 10 hours evaporation and 3.380 for the standard sirup, as shown in Table 3 and Figure 2, due to the prolonged evaporation in deep aluminium or stainless steel kettles. The alcohol extractives of standard sirup has the light color and the residue reddish brown and the absorption spectra have the maxima in wave length of 350 nm, 1,000 nm, 1,170 nm, 1,400 nm and 1900 nm, as shown in Figure 3. The extractives of ethanol and monoethanolamine mixture (97:3, v/v) has dark red and the residue darker redish brown.

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Optimization in the Preparation of Aloe Vinegar by Acetobacter sp. and Inhibitory Effect against Lipase Activity (Acetobacter sp.를 이용한 알로에 식초의 발효조건 및 Lipase활성 저해효과)

  • Ryu, Il-Hwan;Lee, Kap-Sang;Shin, Yong-Seo;Chun, Seung-Ho;Lee, Do-Sang
    • Applied Biological Chemistry
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    • v.42 no.2
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    • pp.105-110
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    • 1999
  • Acetobacter sp. were isolated from persimmon vinegar. We studied about conditions of Aloe vinegar fermentation by an isolated strain and inhibitory effect against lipase activity. Strains DS-118 was strictly aerobic, motile, gram negative, non-spore-forming and short rod shaped. It reacted positively in catalase test, was oxidase test negative, was ureas negative, was produced acetic acid from alcohol. On the basis of these results, it was identified as a strain of Acetobacter sp. In the preparation of Aloe vinegar, optimun initial alcohol concentration, acidity, and fermentation temperature were 10%, $3{\sim}4%$ and $25^{\circ}C$, respectively. The major organic acid in Aloe vinegar was acetic acid (12%), but malic acid and ${\delta}-galactronic$ acid were also present in trace. The Aloe vinegar(acidity : 12%) inhibited lipase activity and it's $IC_{50}$ was 43%.

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Asymmetric Ring Opening Reaction of Racemic Epoxides by Polymeric Chiral Salen Catalyst containing Metal Salts (금속염 함유 고분자형 키랄 살렌촉매에 의한 라세믹 에폭사이드의 광학선택적 비대칭 고리열림반응)

  • Lee, Kwang Yeon;Rahul, B. Kawthekar;Kim, Geon-Joong
    • Applied Chemistry for Engineering
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    • v.18 no.6
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    • pp.562-567
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    • 2007
  • The stereoselective synthesis of chiral terminal epoxide is of immense academic and industrial interest due to their use as versatile starting materials as well as chiral intermediates. In this study, new polymeric chiral Co(salen) complexes bearing tallium (III)chloride and iron (III)chloride (ferric chloride) have been synthesized and characterized. Their catalytic activity and selectivity have been demonstrated for the asymmetric ring opening of various terminal epoxides using water and phenol derivatives as nucleophiles. The easily prepared polymeric complexes exhibited very high enantioselectivity for the asymmetric ring opening of epoxides with $H_2O$ and phenol nucleophiles, providing enantiomerically enriched terminal epoxides (> 98% ee). The system described in this work is very efficient for the synthesis of chiral epoxide, 1,2-diol and ${\alpha}$-aryloxy alcohol intermediates.

Alcohol Use Disorder, Stress, Mental Health and Suicide among Seoul Citizens (서울시 지역주민의 알코올장애와 스트레스, 정신건강 및 자살)

  • Sohn, Ae-Ree;Legaspi, Sherme Villasurda;Hong, In-Ok;Kim, Tae-Kyung;Ryu, Eun-Jung;Oh, Gyung-Jae
    • Korean Journal of Health Education and Promotion
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    • v.26 no.4
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    • pp.71-81
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    • 2009
  • Objectives: This study furthers the currents understanding of alcohol use disorders, stress, mental health status and suicide among Seoul citizens. This study is to identify differences according to socio-demographic variables and variables which affect stress, mental status and suicide for developing mental health programs. Methods: The study subjects using cluster-stratified sampling method were 1234 adults over 19 years old from 17 dong, S-Ku in Seoul City. A cross-sectional study with face-to-face interview was used to collect data. A questionnaire measuring socio-demographic variables, Symptom Checklist-90-Revised (SCL-90-R) and psychosocial wellbeing index-short form (PWI-SF) was utilized. Results: Three findings in this study were discussed: (1) The prevalence of problem drinking and alcohol dependence and AUDIT score were significantly higher among male, younger age, and high level of education and income; (2) AUDIT score were related with stress, all 9 symptoms of SCL-R and suicide plan; (3) Problem drinkers were more likely to have 2.5 times of anxiety, 3.0 times of hostility, and 2.4 times psychoticism than non alcohol problem drinkers. Alcohol dependence drinkers were more likely to have 1.9 times of high risk group of stress, 4.1 times of obsessive-compulsive, 4.7 times of anxiety, 5.5 times of hostility, and 2.3 times psychoticism than non alcohol dependence drinkers. Conclusion: The findings suggest that the future intervention of Mental Health programs should be carefully designed and tailored by socio-demographic variables.

Conservation of Waterlogged Wooden Finds Excavated in Wet-Site (저습지 출토 목재유물의 보존과 현황)

  • Yi, Yong Hee
    • Journal of Conservation Science
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    • v.6 no.2 s.8
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    • pp.126-140
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    • 1997
  • There were some cases in the past that waterlogged wooden finds were neglected and damaged severely because adequate methods of conservation processing could not be found. However, since a wooden ship unearthed in Anapji of Kyongju was processed by poly(ethylene glycol) (PEG) impregnation method in 1975, most of waterlogged wooden finds have been processed by diverse scientific methods. Most commonly-used conservation processing methods of waterlogged wooden finds in Korea are PEG impregnation method, alcohol-ether-resin method and vacuum freeze-drying method. New methods developed recently in Europe and Japan such as sucrose method, sugar-alcohol method and higher alcohol method are also being studied here. The most important task in conservation processing of waterlogged wood is to find good impregnation materials suitable to Korean climate and environments and develop their application methods. For efficient conservation processing, it is important to know the natures of finds and impregnation materials and relation between impregnation and drying condition. To achieve it, many experiments and studies are needed.

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A Hypothesis Study on the Physiological, Psychotic, and Psychological Factors of Vincent van Gogh's Yellow Expression (빈센트 반 고흐의 노란색 표현에 대한 생리적, 정신증적, 심리적 요인에 대한 가설 연구)

  • Oh, Seoung Jin;Ryu, Jung Mi
    • Journal of Naturopathy
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    • v.11 no.2
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    • pp.123-135
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    • 2022
  • Background: The study aims to examine what color representation means to artists by investigating various hypotheses about van Gogh's expression of yellow and verifying the reason of his preference of yellow. Purpose: The purpose of this study is to investigate whether yellow expression of Vincent van Gogh is a result of physiological responses of alcoholism, expression feature of mental disorder, or a problem caused by psychological motivation. Methods: In order to verify the research question, we referred to research literatures that analyzed a characteristics of Gogh's works in a various area such as psychology and psychiatry, and Gogh's symptoms and his own skills. Results: The findings suggested that Gogh's yellow preference is related to the psychological factors such as inner motivation, not a xanthopsia which is brought about alcoholism and mental disorder. Conclusions: Gogh's Yellow expression is dominantly influenced by the psychological factors. Thus, it can say that the psychological factors has a great on characteristic of artist's color expression.

A Study on the Safety of Alcohol-based Hand Sanitizers (알코올을 주성분으로 하는 손소독제의 안전성 연구)

  • Sun-Ok Jung;Chun-Yeong Lee;Hoe-Jin Ryu;Hee-Jin Choi;Ji-Young Kim;Chae-Man Choi;In-Sook Hwang;Yong-Seung Shin
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.33 no.1
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    • pp.34-39
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    • 2023
  • Objectives: In this study, the safety of alcohol-based hand sanitizers (ABHSs) for quasi-drugs and cosmetics was investigated by analyzing the ethanol content, which is an active ingredient with a sterilizing effect, and methanol, which is toxic. Methods: Forty-one ABHSs were purchased at large supermarkets and online stores. Ethanol quantification was performed by gas chromatography-flame ionization detector, and methanol quantification was performed by headspace-gas chromatography-mass spectrometry. Results: The ethanol content of ABHS in quasi-drugs was 49.6-67.8%, which was suitable for standard manufacturing procedures for external disinfectants, and the ethanol content of ABHS in cosmetics was 9.1-61.3%. The methanol content of ABHS in quasi-drugs ranged from not detected(N.D.)-131.8 ppm, which was suitable for the methanol detection standard of ethanol raw materials in the Korean Pharmacopoeia. The methanol content of ABHS in cosmetics was 23.4-859.7 ppm, which was suitable for the detection limit of methanol in cosmetics. Conclusions: The ethanol and methanol content of ABHS was judged to be safe. When selecting an ABHS to be used for sterilization, it seems necessary to check the content of ethanol, an active ingredient, and use it according to its intended purpose.

Preparation of Low Methoxyl Pectins by Pectinesterase in Tangerine Albedo and their Chemical, Physical and Gelling Properties (감귤류 과피내의 Pectinesterase 작용에 의한 Low Methoxyl Pectin 조제 및 특성에 관한 연구)

  • Yoon, Kyung Hee;Yoon, Sun;Lee, Mung Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.1
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    • pp.7-11
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    • 1983
  • The purpose of this study was to prepare low methoxyl pectins (LMPs) by pectinesterase (PE) using waste tangerine peels and to compare the chemical, physical and gelling properties of the prepared pectins with the commercial LMP. The LMPs were prepared by treating albedo with 0.25M $Na_2CO_3$ to activate the PE. PE was then inactivated by heating. The sample was centrifuged and the supernatant was collected. LMP was obtained as precipitate by adding alcohol to the collected supernatant. The amounts of extractible pectins in albedo were 12.71~12.98% on a dry weight basis. Methoxyl contents of LMPs prepared by treating albedo with PE at pH 7.5 for 10min, at pH 8.5 for 10min, 20min and 30min were 5.12%, 4.27%, 3.08%, 1.85% respectively, demonstrating that the methoxyl contents of the preparations decreased as the degree of treatment albedo with PE increased. The acetyl contents of the preparations ranged from 0.09% to 0.12%, the values of which do not interfere with gel formation. The anhydrouronic acid contents of the prepared pectins were in the range of 94.2%~94.8%. The values of viscosity and molecular weights of the prepared LMPs tent to decrease as the degree of PE action on albedo increased. The textural value of the prepared LMP gels determined by Consistometer, Ridgelimeter and Instron denonstrated that the excess treatment of albedo with PE significantly impaired the gelling properties of the preparations.

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Cell Protective Effects of Enzymatic Hydrolysates of Citrus Peel Pectin (귤피 펙틴 유래 효소적 가수분해물의 세포 보호 효과)

  • Kwon, Soon Woo;Ko, Hyun Ju;Bae, Jun Tae;Kim, Jin Hwa;Lee, Geun Soo;Pyo, Hyeong Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.1
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    • pp.75-85
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    • 2016
  • Pectin, a naturally occurring polysaccharide, has in recent years attracted considerable attention. Its benefits are increasingly appreciated by scientists and consumers due to its safety and usefulness. The chemistry and gel-forming characteristics of pectin have enabled to be used in pharmaceutical industry, health promotion and treatment. Yet, it has been rarely used in cosmetics because of its incompatibility with many cosmetic ingredients, including alcohols, and unstable viscosity of pectin gels under various pH and salt conditions. However, low-molecular-weight pectin oligomers have excellent biological activities, and depolymerization of pectin to produce cosmetic ingredients would be very useful. In this study, we attempted the development of cosmetic ingredients using pectin with an excellent effect on human skin. We developed a bio-conversion process that uses enzymatic hydrolysis to produce pectin hydrolysates containing mainly low-molecular-weight pectin oligomers. Gel permeation chromatography was used to determined the ratio of hydrolysis. The molecular weight of the pectin hydrolysates obtained varied between 200 and 2,700 Da. The two newly developed low-molecular-weight pectin hydrolysates, LMPH A and B, had higher anti-oxidative activities than pectin or D-galacturonic. Exposure to UVB radiation induces apoptotic cell death in epidermal cells. Annexin V binding and propidium iodide uptake were measured by flow cytometry to evaluate UVB-induced cell death in HaCaT cells. Both LMPH A and B reduced UVB-induced cell death and increased cell proliferation by 22% and 30% at 0.5% concentration respectively, while pectin had no significant activity. In conclusion, this study suggests that the newly developed low-molecular-weight pectin hydrolysates can be used as safe and biologically active cosmetic ingredients.