• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.1
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• pp.94-106
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• 1990

사람들이나 동물의 소화관의 상재균으로서 중요한 Lactobacillus acidophilus의 여러균주를 사용하여 세포표면의 성상을 조사하였다. 불리한 세포벽의 negative 염색한 표본을 전자현미경으로 검토한 결과 사용한 21주중 15주는 세포벽외층에 단백질로 구성딘 regular array를 생산하고 있음을 알았다. RA를 구성하는 단백질의 분자량은 41KDa-48KDa이었다 아미노사노성 및 Staphylococcus au-reus V8 protease로 한정분해 후 및 N-chlorosuccini-mide로 부분절단후의 peptide map에서 Johnson등의 분류의 subgroup A-1에 속하는 균주가 생산하는 RA단백질은 불균질하였으나 그외 균주의 RA단백질은 같은 subgroup에 속하고 있어도 불균질하였음을 알았다 따라서 RA단백질은 불균질하였으나 그외 균주의 RA단백질은 같은 subgroup에 속하고 있어도 불균질하였음을 알았다 따라서 RA생산의 유무 및 RA단백질의 분자량측정과 peptide mapping을 행하면 지금까지 행해온 DNA-hybridization과 같은 복잡한 절차를 사용하지 않아도 subgroup A-1의 L. acidophilus가 동정 가능함을 알았다 L acidophilus의 세포표면소수성은 일반적으로 RA를 생산하지 않는 균주보다는 RA를 생사하는 균주에 높은 것이많았다 RA생산균주에 있어서는 RA단백질은 세포표면소수성과 직접관계하고 있는 것은 적었다.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.282.2-282.2
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• 2014

Cr의 함량과 주입 가스의 유량 비율을 변화하여 ZnCrO 박막의 구조적 특성에 미치는 영향과 그것이 자기적 특성에 관여하는 상관관계 등에 관하여 조사하였다. ZnCrO 박막을 Pt/Ti/Al2O3 기판 위에 Sputter법으로 증착하였으며, 이 때 양질의 ZnCrO 박막을 제작하기 위하여 산소 분압 변화에 따른 박막의 표면 및 구조적 특성을 관찰하였다. 아르곤과 산소 분압이 1 : 1 (Ar : O2=15 sccm : 15 sccm) 이었을 경우, 가장 매끄러운 표면을 갖고 또한 구조적으로도 안정된 ZnCrO 박막을 얻을 수 있었다. 동일한 조건에서 Cr 함량을 변화시켜 본 결과, Cr 함량이 작아질수록 자기적 특성이 향상되는 것이 관측되었다. 즉, Cr의 함량이 감소할수록 Cr 입자 1개 당 유효 자화도가 증가하는 것이 관측되었는데, 이러한 결과는 Cr 함량 변화에 따는 ZnCrO 박막의 결정 자기이방성 변화에 따른 것이다.

• The Transactions of the Korean Institute of Power Electronics
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• v.5 no.6
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• pp.610-619
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• 2000

This paper presents an off-line UPS using the high temperature superconductive magnetic bearing. FES(Flywheel Energy Storage) system has good advantages in compare with lead acid battery. So, high efficiency FES using high temperature SMB(superconductive magnetic bearing) was composed in this paper. The outer rotor type of PMSM(Permanent Magnet Synchronous Motor) as motor/generator was used for the experiment, and square wave current and sinusoidal wave control methods was compared for high efficiency operation of motor/generator. The circuit for in phase sinusoidal wave current control with EMF in the full speed range was designed and the proposed flywheel energy storage system was applied in single phase off-line UPS system. As the stable operation characteristics of prototype system was confirmed, the its excellence as energy storage device in Off-line UPS was proved.

• KIEAE Journal
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• v.12 no.1
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• pp.105-112
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• 2012

When a building is planned and designed, the design should be able to minimize the cost during the whole life cycle of the building. This study has begun to analyze LCC about the alternative design which is applicable to renewable energy facility construction. It is reviewed domestic and foreign papers about the trend of LCC technology and it is determined the analytical method to analyze the LCC of renewable energy. Regarding the review of alternatives, it is chosen the three alternatives which are able to designed combing the renewable energy facilities and it is performed the LCC analysis about each alternative. Alternative 1 is Photovoltaic + Solar Thermal + Photovoltaic /Wind Power, Alternative 2 is Geothermal + Photovoltaic, and Alternative 3 is Photovoltaic + Solar Thermal. The LCC analysis is present value method, its analytical period is 40 years and it is applied 3.2% of real discount rate. As a result, it is proved that Alternative 1 and Alternative 3 are not able to collectible the early investment cost during the analytical period and Alternative 2 is analyzed that its pay-back period of early investment cost is about 31 years. As the final outcome of this study on case analysis, it is more advantageous to use the combination of Geothermal and Photovoltaic energy than to use the other combination in LCC aspect.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.35 no.2
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• pp.156-160
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• 1999

This experiment was carried out to investigate the auditory threshold of the scorpion fish Sebastiscus marmoratus which was suitable for Marine ranching by a classical respiatory conditioning technique using a sound coupled with a delayed electric shock. The thresholds were determined by analyzing the electrocardiogram. The auditory thresholds were observed among the 12 fish with much difference from 100Hz to 300Hz. The audible range of the scorpion fish extended from 80 to 800Hz with a peak sensitivity of 90㏈(0㏈= $1\mu$Pa) at 100Hz. As the frequency became higher than 300Hz, the auditory threshold increased rapidly. The scorpion fish was least sensitive to sound of 500Hz among 6 frequency points and the value was about 12㏈.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.44 no.2
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• pp.148-156
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• 2008

In this study it will be discussed how to solve the problem of discomfort from rolling motion on the fishing boats. Most discomfort is caused by the short cycle of rolling due to the light weight of the boats. The light weight is due to the FRP material which dries a boat. A way to improve the feeling of boarding by using fish hold was researched. The experiment was done on experimental fishing boat made by FRP in Jeju. An existing fish hold was designed and manufactured through the rolling test and that was used for a marine experiment. The rolling condition of the U-tank boat ideally designed was compared to that of an existing fishing boat using the same conditions. The experiments were carried out two times on the stop engine in the outward Sehwa fishing port, which the experimental data had analysed for effects of rolling reduction to compare the U - tank with the of exiting fish hold. The results were confirmed that the U-boat tank in the roll period and GoM were more safe than the existing fish hold and the average amplitude and significant of rolling angles were decreased relatively.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.3
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• pp.213-221
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• 2005

In order to get more accurate GPS position with the changes of the inner configuration setting of GPS receiver, the authors carried out measurements of the position at known it with one antenna and two GPS receivers manufactured by same company. We have investigated the accuracies of positions according to the change of the maskangle and receiving mode of output data in inner configuration of GPS receivers, and analyzed the relationships between numbers of satellites visibility and maskangles, and values of HDOP and maskangles. When the maskangles in inner configuration were set below 20 degree, the accuracies of positions were high. But if they were became bigger than 25 degree, standard deviations ot position errors and HDOPS of positions were became bigger. Numbers of satellites visibility(y) and maskangles(x) have relations with a formula, y = -0.1662x+9.9225, and values of HDOP(y) and maskangles(x) have relations with a formula, y = 0.6035 $e^{0.0517x}$. The results of position accuracies observed by two GPS receivers to the known position at same time were that average errors of position fixs by GPS receiver configured with NMEA0183 mode were 6.7m and standard deviations were 1.5m, and them by GPS receiver configured with binary mode were 5.0m and standard deviations were 1.1m respectively.

• Journal of ILASS-Korea
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• v.15 no.4
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• pp.177-181
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• 2010

This paper has designed a mixing enhancement apparatus called Lobed-plate and Step-plate and comparatively calculated desulfurization efficiency of when its shape was changed. The parameters used at this time were the shape, SR ratio and the number of nozzles of the mixing enhancement apparatus and comparatively analyzed desulfurization efficiency according to these parameters. As a result, the Step-plate appeared as more highly by around 4% than Lobed-plate in desulfurization efficiency according to the shape of the mixing promotion apparatus, and when the desulfurization efficiency as a SR ratio is considered, it appeared highly by an average of 5% when the SR ratio is 3 rather than 2. As a result of comparing desulfurization efficiency by fixing the SR ratio and setting the number of nozzles as 4 pieces and 6 pieces, there was no big change in desulfurization efficiency when the SR ratio is 2, but it could be confirmed to improve by around 5% when the SR ratio is 3 when time passed 8 seconds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.134-140
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• 1997

The monoclonal antibody against a novel protein, which was induced in the roots of rice seedlings treated with NaCl, was produced. Oryzea sativa L. cv. Annapruna grains were sown on clean sands and grown for 10days and then the seedlings were soaked in 50mM NaCl aqueous solution. In 48hrs of the NaCl treatment, the roots were collected and homogenated with liquid nitrogen and extraction buffer. The homogenate was centrifuged and to the supernatant 75％ ammonium sulfate was added to pre-cipitate proteins. From these proteins a novel protein was purified through DEAE-ion chromatography and FPLC(Phenyl column). This protein appeared as a single band in the native electrophoresis. Using this protein as antigen, monoclonal antibody was produced. Five cell lines that secreted antibodies specifically bound to this protein were constructed.

• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.344-351
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• 1990

An alkalophilic microoganism producing a detergent-resistant alkaline protease was isolated from soil and identified as Baeiltus sp. The alkaline protease has been partially purified by ammonium sulfate fractionation, DEAE-Cellulose, CM-Cellulose and Sephdex G-100 column chromatography. The purified alkaline protease was highly active at pH 12-13 toward casein and stable at pH values from 6 to ll. The optimum temperature for the enzyme reaction was $55^{\circ}C$. The enzyme was completely inactivated by diisopropyl fluorophosphate (DFP) indicating that the enzyme was serine protease, but considerabiy stable in the presence of surface active agents.