• Journal of fish pathology
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• v.9 no.2
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• pp.113-118
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• 1996

During a study to document more fully the parasitic ciliates of Korean freshwater fishes, we found numerous small ciliates in the gills and skin of crucian carp, Carassius carassius. Cursory examination indicated that the ciliate were very similar with Chilodonella sp. In this study, we described the morphometrics of this ciliate in detail and compared with other species of Chilodonella in the world. Numerous small ciliates were observed in the body surface, fins and gills of infected fish and excessive mucus production were seen in those fish. Sometimes ulcer was observed in the body of moribund fish. From the scrutinized observation of the parasite, it was identified as Chilodonella hexasticha. The parasite was dorsoventrally flattened body, without a notch in the posterior margin, and it measured 30-$45{\mu}m$ long and 15-$30{\mu}m$ wide. In number of kineties, the right band ciliature was 3-5 and the left band ciliature was 3-5. The right ventral ciliary band shaped arch and was longer than the left, straight band. It had a single oval macronucleus, 8-$15{\mu}m$ in diameter, a single micronucleus, 2-$4{\mu}m$ in diameter. The cytopharynx was reinforced by 10-16 conspicuous nematodesmata, which shaped like a tube and curved at its inner end. Two contractile vacuoles, one anteriorly at right and the second posteriorly at left were observed in wet mounts. This parasite reproduced by a simple division at $22{\pm}1^{\circ}C$.

• Journal of fish pathology
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• v.9 no.2
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• pp.119-126
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• 1996

We report on the variation of infection and histopathological change of Microcotyle sebastisci parasitic on cultured rockfish, Sebastes schlegeli, in Namhae Islands and Kamak Bay from April to October in 1995. Microcotylosis due to Microcotyle sebastisci occurred among cultured rockfish, Sebastes schlegeli. This parasite was not found on the host fish from June to July. Parasite sites were mainly consist of 2nd and 3rd gill arch`s filaments of rockfish. Also, the sites were secreted in large quantity of mucus with a very small bleeding. In Namhae Islands, maximum values of prevalence, relative density and mean intensity were found on September 1995, as 40.0%, 30.7 and 76.8, respectively. In Kamak Bay, maximum values of prevalence, relative density and mean intensity were obtained on October 1995, as 46.0%, 40.5 and 88.0, respectively. Histopathological changes of the heavily infested gills were showed necrosis, epithelium of the gill filaments underwent hyperplasia with fusion of the lamella and filamental clubbing. And a bacterial colony is invaded on the surface of lamella epithelium.

• Parasites, Hosts and Diseases
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• v.23 no.2
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• pp.221-229
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• 1985

The echinostomatid metacercariae encysted in the gill of the fresh water fish, Pseudorasbora larva were identified through obtaining adult worms after eBperimental infection to mice. In addition, a brief course of worm development and maturation was observed in this experimental host. The results were as follows: 1. The echinostomatid metacercariae were elliptical, golden yellow, 0.073∼0.078 mm long and 0.0541∼0.065 mm wide. Their head portions were characterized by the presence of a head crown armed with collar spines of total 24 in number and interrupted at the mid-dorsal side of the oral sucker. 2. The average rate of worm recovery froth 12 mice (on the 1-2lth postinfection days) was 19.4 % and the rate revealed no decrease in accordance with the increase of infection duration. The worms were collected chiefly from the lower part of the small intestine. 3. After the infection, their sexual maturation was attained in 5 days and their growth in size nearly completed in 7 days. The early growth curve of genital organs was S shape while that of nongenital organs was C form. In 5 day old worms, 1 or 2 eggs were found from their uteri and the stools of mice revealed echinostomatid eggs from the 5-6th postinfection day. 4. The 7 day old adult worms were ovoid in shape, 0.54-0.69 mm long and 0.29-0.34 mm wide, and characterized by a well developed head crown with 24 collar spines and vitelline follicles distributed from the acetabular level down to the posterior end of body. Based on these characters they were identified to be Echinochasmus japonicus Tanabe, 1926. From these results, it is verified that p. larva is one of the second intermediate hosts of 5. jatonicus in Korea.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.4
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• pp.234-237
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• 2007

Calmodulin (CaM) is a $Ca^{2+}$-binding protein essential for biological functions mediated through $Ca^{2+}$-dependent mechanism. A cDNA clone for CaM was isolated from a cDNA library of olive flounder Paralichthys olivaceus. The CaM cDNA concists of 782 bp and encodes a polypeptide of 149 amino acids with four $Ca^{2+}$-binding motifs EF-hands (EF-I, EF-II, EF-III, and EF-IV). The deduced amino acid sequence of CaM shows 97-100% amino acid sequence identity to other CaM sequences. Semi-quantitative PCR analysis revealed that the CaM transcription was began during early development and the CaM mRNA is expressed highly in brain and intestine, and moderately in kidney, gill, and eye of healthy olive flounder. Taken together, CaM may be necessary for early olive flounder development and that it may have a part in homeostasis.

• Journal of Life Science
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• v.19 no.8
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• pp.1112-1118
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• 2009

In the present study, the effects of muddy water were compared and examined on the fish inhabiting Imha reservoir, E. erythropterus, H. labeo and Z. platypus in Cypriniformes, S. asotus in Siluriformes and S. scherzeri in Perciformes. In the gills of fish in muddy water, the secondary lamellae of fish in Cypriniformes showed more irregular gaps and more winding than those in Siluriformes and Perciformes. Also, the edema and detachment of epithelial cells was more detected in the gills in Cypriniformes, but clubbing was frequently detected in Perciformes and Siluriformes. Also, we found that muddy debris and minute particles attached to lamellae were frequently detected in Perciformes and Cypriniformes. The glomerulus in the kidney was much more constricted in Z. platypus in Cypriniformes. As a result, it is proposed that fish in Cypriniformes are more affected by muddy water. In the changes of blood components, which might be affected by muddy water, the concentrations of TP, ALB, TG, CHOL and A/G ratio, were high in Cypriniformes, and inorganic matters were abundant in Siluriformes. In addition, AST activity was relatively higher than ALT and both activities were higher in Cypriniformes than in Siluriformes. Taken together, the change in blood plasma may be caused by the reduction in voracity in the wake of muddy water, which results in tissue damage.

• Journal of the Korean Society for Marine Environment & Energy
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• v.10 no.1
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• pp.21-28
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• 2007

The bioaccumulation of Pb and Cd dissolved in seawater was assessed measuring the concentrations recorded within blue mussel (Mytilus edulis) and oliver flounder (Paralichthys olivaceus) after two weeks exposure period. The Pb and Cd concentration within the whole body of two testing organisms increased according to the exposure concentrations, and the such tendency was clear specially from the mussel. Maximum metal concentration reached $5,260({\pm}70)\;{\mu}g/g$ for Pb reared under 5.0 mg/L Pb, $1,040({\pm}40)\;{\mu}g/g$ for Cd reared under 1.0 mg/L Cd in the mussel, and indicated that the bioaccumulation of Pb and Cd was directly related to the rearing medium concentrations. Bioconcentration factors (BCF) reached very high values for Pb (maximum value: $12,100{\pm}1,400$) in the mussel reared under lowest Pb concentration (0.01 mg/L). The BCF value for Cd in the mussel were also far higher at exposure to low Cd concentration than high Cd concentration. At higher external concentrations, the BCF for Cd and Pb declined. This demonstrated the ability of two testing organisms to rapidly uptake heavy metals particularly when exposed to low external concentration. The mean Pb concentration was slightly higher in the gill of mussel than in the digestive gland, while Cd showed a higher level in the digestive gland than in the gill.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.1
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• pp.27-32
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• 1970

A study on the purification of the surf clam Mactra Sulcataria REEVE for the remove of sand and fecal piles was conducted in the laboratory. The sand was mostly distributed around the inhalent and exhalent siphon, mantle and gill, and were also distributed in the digestive tract including the mid-gut gland. The sand particles in the digestive tract were extremely small and their sizes were about $180\times10\mu\;to\;550\times200\mu$. It could be seen that there was little, if any, difference in the rate of sand removal when either the hanging Purification method was used or the method of laying the surf clams in thick or thin layers on the bottom of the experimental vessel was used. The surf clam removed about $50\%$ of its sand during the first hour of purification. The weight of the removed sand and fecal piles on a dry basis reached a constant value after 22 to 26 hours of purification. After 42 hours of Purification, no sand could be seen in the fecal piles. The surf clam removed the sand in its body more rapidly when it was in sea water with the pH of 8.75 than when it was in natural sea water. Also high temperature had a much greater depressing effect upon the removal of sand In the surf clam than did natural sea water. Also low salinity had greater accelerating effect upon the removal of sand in the body than did natural sea water. However the surf clam died when the salinity was $8.19\%_{\circ}$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.581-585
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• 1986

A growth experiment of the Israeli strain of common carp (Cyprinus carpio) under varying dissolved oxygen levels in the recirculating water system was conducted at the Fish Culture Experiment Station of the National Fisheries University of Pusan from August 28, 1985 to September 17, 1985. Five tanks with a capacity of $5m^3$ of water each were used under the same condition of water parameters except for dissolved oxygen levels which were designed to maintain at 2.0, 2.5, 3.0, 3.5 and 4.0 mg/l ranges. The weight of fish in the beginning was about 300g and each tank was stocked with 200kg of fish. DO level of 3.5mg/l was found to be the best level with a feed coefficient of 1.57 and a daily growth rate of $1.411\%$ whereas 4.mg/l showed a slightly decreased performance of 1.63 and $1.365\%$ respectively. The amounts of feed consumed in 3.5 and 4.0mg/l DO levels were almost the same. Below 3.0mg/l DO levels the growth rate markedly decreased. Furthermore, in 2.0 and 2.5 mg/l groups, the fish did not accept feed vigorously and after feeding the fish usually concentrated around the inflow point showing oxygen deficiency response, The experiment indicates that the DO range of 3.5 to 4.0mg/l is the optimum level for the best growth at $27.5^{\circ}C$. DO concentration above these levels is considered a waste of energy resulting in uneconomical performance, and on the other hand, below these levels, the carp certainly shows a poor growth performance.

• Korean Journal of Ichthyology
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• v.20 no.4
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• pp.285-290
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• 2008

Morphological characteristics of the cross-bred $F_1$ hybrid between female Paralichthys olivaceus and male Platichthys stellatus were studied. The hybrids showed common biological characteristics of both parents, but showed more morphological similarity to P. stellatus than P. olivaceusin the following biometries: higher ratios of body length to body height (56.3%), head length to snout length (20.6%), and head length to upper jaw length (34.2%). They also showed some significant biometric characteristics similar to P. stellatus, such as ratios of predorsal length (10.5%), prepectoral length (32.2%), preventral length (29.8%), and preanal length (28.0%) to body length. The hybrids had black spots on the dark brownish-green area near the eyes, and they lacked the small bony tubercles that area characteristic of Platichthys stellatus. The hybrids had unique bands: 6~20 bands on the dorsal fin and 5~10 bands on the anal fin that gradually lost their coloration towards the fin margins. The number of fin rays were 60~66 for dorsal fin, 40~51 for anal fin, 18~21 for caudal fin, and 11~12 for pectoral fin. The gills of the hybrids contained 13~18 gill rakers and 73~98 gill filaments. The vertebrae of the hybrids numbered 34~37, with 2~3 vertebrae fused from the start of the caudal vertebrae.

• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.3
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• pp.207-212
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• 1986

Heavy metal contents and residues level of organochlorine pesticides were analyzed for commercial salted and fermented sea-food which bought from pusan area. Heavy metal contents ranged from 0.11 to 0.46 ppm of mercury, trace to 1.1 ppm of lead, 0.01 to 0.34 ppm of cadmium, 0.4 to 5.98 ppm of cupper, 0.16 to 4.5 ppm of zinc, trace to 0.49 ppm of manganese and trace to 1.35 ppm of arsenic. Residue level of organochlorine pesticides in salted and fermented sea-foods ranged from 0.3 ppb to 1.825 ppb of total BHC Organochlorine pesticides of DDE, dieldrine, pp'-DDD and pp'-DDT were detected a small amount from Ge jeot and Myeol-Chi jeot. In conclusion. the heavy metal and levels of organochlorine pesticides residues were safety to eat the salted and fermented sea foods.