• The Korean Journal of Malacology
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• v.31 no.2
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• pp.143-150
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• 2015

This study was conducted to find out accumulated concentration of nickel, survival rate and falling rate, structural changes of abalone, Haliotis discus hannai exposed to nickel chloride. Experimental groups were composed of one control condition and five nickel chloride exposure conditions (5.9, 8.8, 13.3, 20.0, 30.0 mg/L). The accumulation of nickel in abalone was significantly increased all exposure group. Though the exposure groups had lower survival rate than the control group, higher falling rate than the control group. Histopathological changes in the foot, gill and hepatopancreas of abalone revealed obvious with exposure group than control group.

• The Korean Journal of Zoology
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• v.21 no.3
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• pp.87-102
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• 1978

Electron microscopic studies were made to investigate changes in the fine structure of the liver, kidney and gills of Carassius carassius L. following exposure to 1 and 2.5 ppm of $HgCl_2$. The following results were obtained: 1. In the mercury-treated liver cells, an increase in the number of lysosomes were noticed. These lysosomes appeared to be of two types; round ones containing some crystalline structures and others with phagocytosed glycogen granules and mitochondria. Also observed were mitochondrial swelling where the matrix appeared less electrondense, and segregation of the nucleoli in the nucleus. 2. In the kidney, mercury treatment resulted in thickening of the basement membrane of the glomerulus, and appearance of vacuoles and cytoplasmic bodies in the proximal convoluted tubule. The vacuoles seemed to be formed from mitochondria. Nuclear shrinkage was also noticed at 2.5 ppm of $HgCl_2$. 3. Many large and small lysosomes appeared in response to mercury in the epithelial cells of the gill lamella. Also the lamellar membrane became fuzzy in appearance. 4. It can be concluded from these results that mercury-induced changes in the fine structure are associated with activation of detoxication processes and impairment of energy metabolism.

• The Korean Journal of Zoology
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• v.4 no.1
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• pp.7-12
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• 1961

1) Respiratory metabolism patterns and its enzyme systems in the gill tissue of the fresh water mussels, Cristaria plicata were investigated through the examination on the effects of respiratory enzyme inhibitors, (KCN, NAF) and succinoxidase assay, while studying the effects of neutral salts (NaCL, KCL, CaCl2) and pH on oxygen consumption of the gill tissue. 2) In the limited concentration of KCL (0.3mM) and NaCl (0.4mM) solutions, oxygen consumption of the intact gill tissue was accelerated, but in CaCl2(0.5mM) solution, it showed no significant effect. The oxygen consumption was gradually decreased at the above concentrations of these limitations. The optimum pH for the respiration of the gill was 7.3. 3)Cyanide in 10-8M solution inhibited 88.8% of the respiration of the intact gill tissue. Methylene blue accelerated the respiration of the noral gill tissue, and slightly but significantly reversed the cyaniide poisoned respiration. 4)Oxygen consumption of the gill homogenate was apparently increased by the mixed addition of succinate, cytochrome c and activators (AlCl3 and CaCl2). This results suggested that succinoxidase system acts on the respiratory pattern of the gil tissue. 5) It was able to recognize that the enolase, which acts on the anaerobic glycolytic system, participated in the tissue respiration of the gill for NaF in 5$\times$10-2 M solution inhibited 55.5% of the respiration of the same intact tissue.

• Korean Journal of Ichthyology
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• v.25 no.4
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• pp.239-243
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• 2013

Two specimens of the syngnathid Syngnathoides biaculeatus were collected from the coastal waters of Geoje Ialand and Tongyeong, Korea, represent first record of the species and the genus from Korea. This species is characterized by the following combination of characters: caudal fin absent; opercle without keel; superior and inferior trunk ridges continuous with their respective tail ridges; lateral trunk ridge deflected dorsal behind anal ring. Tail shorter than head and body hexagonal in its subdorsal part, posterior quadrangular, rapidly tapering, without caudal fin, prehensile. Head essentially in line with longitudinal axis of body or bent very little. New Korean names are proposed, "Jal-pi-sil-go-gi-sok" for the genus Syngnathoides, and "Jal-pi-sil-go-gi" for S. biaculeatus.

• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.640-644
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• 2010

하천, 호소 등에서 공사 시 준설작업으로 인해 고탁수가 발생되며 이러한 탁수의 이송 확산은 수환경에 변화를 초래한다. 탁수는 준설작업 시 커터헤드(cutter head)가 해저면에 닿는 순간부터 작동을 멈출 때까지 계속하여 발생하며, 이러한 과정들이 반복되면서 많은 양의 부유토사가 발생하게 되고 고탁수현상이 일어난다. 이렇게 발생한 탁수는 수체흐름에 따라 이송 및 확산된다. 탁수발생은 수중의 빛 투과를 감소시켜 일차 생산자인 부유성 및 부착조류의 생육을 저해하고, 이들의 생산성 감소와 군집구성의 변화는 수서생태계의 먹이사슬을 통해 이들을 먹이로 하는 저서무척추동물과 어류의 현존량 감소와 종 구성에 영향을 미치고, 고농도의 현탁 입자는 어류 아가미에 염증을 유발하거나 점막의 파괴와 감염을 유발하여 치사시킬 수도 있다. 또한 과도한 부유 입자는 하류로 침강되어 하천 바닥에 서식하는 부착조류, 무척추 동물 및 곤충의 생육에 피해를 주고, 이것은 어류의 먹이에 영향을 미쳐 어류 개체수를 감소시키거나 산란된 물고기 알을 매몰시키거나 질식시키는 등 여러 가지 방법으로 수서생물상에 영향을 미치게 된다.(낙동강수계관리위원회, 2005) 따라서 준설작업에 있어 탁수의 이송 확산범위를 사전에 예측하고 국내 실정과 환경여건에 알맞게 적용되고, 실용화될 수 있는 수치모델링에 대한 기반핵심 기술개발이 필요하다. 현재 낙동강에서 진행되고 있는 준설현장에서 발생하는 부유탁수의 이송 확산과정을 이차원 흐름해석모형인 RAM2 모형과 오염물 이송 확산해석모형인 RAM4 모형을 이용하여 수치해석을 하고 분석함으로써 수치해석에 의한 부유탁수의 이송 확산모의 결과가 환경영향 범위를 예측하는 데에 적용될 수 있는지를 알아보고자 한다.

• Journal of fish pathology
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• v.17 no.1
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• pp.21-28
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• 2004

In late summer of 2001, a new disease "overturn disease" occurred among mud loach (Misgurnus mizolepis) cultured in a farm Chonbuk Province. The fish exhibited a lethargic, erratic and spiral swimming at the water surface. Affected fish showed swollen abdomen with accumulation of air bubbles in the intestinal canal. The bubbles were much larger in the anterior part than in the posterior part. Hematological and histopathological examinations were done. No significant differences were found in RBC count, Ht value and Hb concentration between the diseased and the healthy fish. Gill filaments showed proximal hyperplasia of the respiratory epithelia and fusing of the gill lamella. Hemorrhage and necrosis occurred in the intestinal epithelia and within the lamina propria, and the mucosal epithelia were separated from the muscularis. Hepatocytes underwent atrophy. In the fish experimentally tied between the annal fin and the anus by a thread to confirm accumulation of air bubbles within intestine, accumulation of air bubbles was confirmed. This disease in mud loach appeared to be induced by circulatory disturbances in the intestinal wall owing to accumulation of air bubbles in the alimentary canal after fed high protein diet throughout all culturing period.

• Korean Journal of Ichthyology
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• v.33 no.2
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• pp.142-147
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• 2021

A single specimen (52.5 mm SL) of Neoclinus chihiroe, belonging to the family Chaenopsidae, was firstly collected from Dokdo, East Sea of Korea on 9 December 2020. This species was characterized by a black spot between the first and the second dorsal spines, and a black spot on upper part of opercular membrane. The species was morphologically similar to N. okazakii, but distinctly distinguished by pectoral fin base without a black dot. This study documents the first record of N. chihiroe in Korean waters and suggests the new Korean name 'Dong-hae-bi-neul-be-do-la-chi' for the species.

• Journal of Marine Life Science
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• v.5 no.1
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• pp.17-24
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• 2020

We have identified a heat shock protein 70 gene from freshwater snail, Semisulcospira coreana. The freshwater snail HSP70 gene encode a polypeptide of 639 amino acids. Based on bioinformatic sequence characterization, HSP70 gene possessed three classical signature motifs and other conserved residues essential for their functionality. The phylogenetic analysis showed that S. coreana HSP70 had closet relationship with that of golden apple snails, Pomacea canaliculata. The HSP70 mRNA level was significantly up-regulated in response to thermal and salinity challenges. These results are in agreement with the results of other species, indicating that S. coreana HSP70 used be a potential molecular marker in response to external stressors and the regulatory process related to the HSP70 transcriptional response can be highly conserved among species.

• KSBB Journal
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• v.23 no.5
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• pp.375-380
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• 2008

We made an experiment of keeping extension of raw and semi-dried flounder (Pleuronectes herzensteini). Effect of with(WG) or without gill (OG), drying degree (20% drying：20D, 40% drying：40D) and storage temperature (5 and $10^{\circ}C$) and 0.1% chitosan-ascorbate (CA) treatment of vacuum packaging flounder on growth of contaminated microorganism during storage for 10 days were investigated. Total aerobacter (TA) in the OG-treated raw flounder was $0.3{\sim}0.5$ log cycle lower than that of WG-treated flounder and also, number of coliform (CF) and E. coli (CO) in OG were lower compared with WG. Number of TA,. Especially, the TA was $0.42{\sim}1.20$ log cycle lower compared with raw flounder. The TA of the raw flounder stored at $5^{\circ}C$ compared to $10^{\circ}C$ was $0.6{\sim}1.3$ log cycle lower. The growth of total aerobacter, coliform (and E. coli separated from raw flounder in tryptic soy broth were completely inhibited by 0.1% CA. But the growth of TA in the raw and 20% dried flounder was 1.5 log cycle inhibited by 0.1% CA, and the growth of CF and CO were also slightly inhibited. We did value raw sample that treated CA 0D and 20D, and did vaccum packaging at the $10^{\circ}C$ for 10 days, Sensory quality such as appearance, freshness, and texture and overall acceptability after cooking of the 0.1% CA-treated raw and 20% dried flounder were evaluated from good to very good, while CA non-treated products were evaluated to moderate.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.130-142
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• 2007

This study was to examine impacts of turbid water on fish community in the downstream of Yongdam Dam during the period from June to October 2006. For the research, we selected six sampling sites in the field: two sites were controls with no influences of turbid water from the dam and other remaining four sites were the stations for an assessment of potential turbid effects. We evaluated integrative health conditions throughout applications of various models such as necropsy-based fish health assessment model (FHA), Index of Biological Integrity (IBI) using fish assemblages, and Qualitative Habitat Evaluation Index (QHEI). Laboratory tests on fish exposure under 400 NTU were performed to find out impact of turbid water using scanning electron microscope (SEM). Results showed that fine solid particles were clogging in the gill in the treatments, while particles were not found in the control. This results indicate that when inorganic turbidity increases abruptedly, fish may have a mechanical abrasion or respiratory blocking. The stream health condition, based on the IBI values, ranged between 38 and 48 (average: 42), indicating a "excellent" or "good" condition after the criteria of US EPA (1993). In the mean time, physical habitat condition, based on the QHEI, ranged 97 to 187 (average 154), indicating a "suboptimal condition". These biological outcomes were compared with chemical dataset: IBI values were more correlated (r=0.526, p<0.05, n=18) with QHEI rather than chemical water quality, based on turbidity (r=0.260, p>0.05, n=18). Analysis of the FHA showed that the individual health indicated "excellent condition", while QHEI showed no habitat disturbances (especially bottom substrate and embeddeness), food-web, and spawning place. Consequently, we concluded that the ecological health in downstream of Yongdam Dam was not impacted by the turbid water.