• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 1999.11a
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• pp.127-133
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• 1999

본 논문의 연구내용은 동양의 사상중의 하나인 음양오행의 오행속성을 물리적으로 해석하여 사람의 오장육부의 크기에 따른 체질 분류에 기초하여 수행하였다. 이러한 체질분류에 의하여 생리적 증상, 걸리기 쉬운 질병, 그리고 성격상의 특징과 장단점을 파악 할 수 있었다. 이러한 일련의 연구에 대한 본 실험실의 지속적인 연구 작업의 하나로 MMPI (Minnesota Multiphasic Personality Inventory)로 일컬어지는 미네소타 다면적 인성검사 설문문항에 대한 음양오행적 분석을 시도하였다. 대표적인 분류의 예를 들면 간이 큰 사람이 건강이 좋지 않을 때 나타날 수 있는 대표적인 심리적 증상으로는 욕설을 잘하며, 심장이 약하여지면 상사병에 걸릴 가능성이 커지며, 폐와 대장이 나빠진 사람은 울기를 잘하며, 그리고 위장이 나빠진 사람은 공상을 잘 하고, 신장방광이 나빠지면 겁이 많아지고 생명력을 나타나는 심포삼초의 기능이 떨어지면 불안하고 초조하여지는 심리적 성격과 연결되는 것이다. 이러한 방법으로 분석하였을 때 건강, 성격, 습관 등에 관련된 MMPI 566문항 중 많은 부분에 대하여 흥미롭고, 성공적인 분석을 내릴 수 있었다.

• Journal of Korean Orthopaedic Sports Medicine
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• v.7 no.1
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• pp.45-49
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• 2008

Purpose: To evaluate the recovery time of biochemical changes of body after half-course marathon running. Materials and methods: Thirteen amateur half course marathon runners (12 males and 1 females) were studied. Their average age was 44 years old (range: $38{\sim}54$). Biochemical parameters with blood test including AST, ALT, CK-MB, Treponin, BUN, Cr Na, K were evaluated at finish line, 2nd days, 2nd weeks after running. Results: All the biochemical changes were within normal range throughout recovery time, AST reached its maximum level at finish line and continued until 2nd day after running and returned its pre-running level at 2nd week's test. CK-MB reached its maximum level 2nd day after running and recovered at 2nd week's test. Na, K, BUN and Cr reached to the maximum level at finish line, and recovered to pre-running level at 2nd day's test. Conclusion: In case of half-course marathon, the changes of the kidney enzymes due to dehydration were recovered after 2nd day. And the biochemical indicators of muscle fatigue recovered after 2nd week. It needs at least 2 weeks rest after half-course marathon to recover all the biochemical parameter of the body.

• Clinical and Experimental Pediatrics
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• v.45 no.3
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• pp.413-417
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• 2002

Gitelman's syndrome is an autosomal recessive disorder characterized by hypokalemic metabolic alkalosis, hypomagnesemia, and hypocalciuria that has recently been reported to be linked to thiazide- sensitive Na-Cl cotransporter gene mutation. In this study, we performed renal clearance studies to differentiate Gitelman's from Bartter's syndrome and to confirm the diagnosis in two patients clinically diagnosed with Gitelman's syndrome. Each patient was hydrated by 20 mL/kg body weight of oral water within 30 minutes, which was followed by intravenous half saline. When urinary flow reached 10 mL/min, samples of urine and serum were obtained to calculate the osmolar clearance, free water clearance, chloride clearance, and distal fractional chloride reabsorption. Subsequently, furosemide or hydrochlorothiazide was administered. Samples were collected and the same parameters were calculated. In our patients, chloride clearance was increased more than 10 times after furosemide administration(2.1 : 25.7 and 2.2 : 27.4 mL/min/100 mL GFR), but not increased after hydrochlorothiazide treatment(2.1 : 1.6 and 2.2 : 2.6 mL/min/100 mL GFR). And the distal fractional chloride reabsorption was significantly decreased by furosemide injection (73% : 15% and 75% : 4.6%), whereas hydrochlorothiazide had no effect on it(73% : 63% and 75% : 78%). These findings indicate that our patients have a defect in thiazide-sensitive Na-Cl cotransporter in the distal tubule, which is compatible with the pathophysiology of Gitelman's syndrome.

• Korean Journal of Ichthyology
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• v.22 no.1
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• pp.17-24
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• 2010

The present study investigated the expression of glucocorticoid receptor (GR) mRNA as a stress response during salinity changes (35, 10, and 0 psu) and water temperature changes (from $20^{\circ}C$ to $30^{\circ}C$, $1^{\circ}C$/day) in black porgy. We cloned the full-length GR cDNA from the kidney and examined its expression in the gill, kidney, and intestine by quantitative real-time PCR (QPCR) during salinity and water temperature changes. During salinity changes, the levels of GR mRNA in the gill, kidney, and intestine were highest at 0 psu, and the levels of plasma cortisol and glucose were elevated, but triiodothyronine ($T_3$) decreased. Also, during water temperature changes, the levels of GR mRNA in the gill, kidney, and intestine increased at $30^{\circ}C$. Plasma parameters also increased with an increase in water temperature. Therefore, this upregulation of GR mRNA was a good indicator of stress, such as those resulting from changes in salinity and water temperature.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.8-14
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• 2001

The effects of media, cholesterol, $\beta$-sitosterol and lecithin on the growth and oospore production of the isolates KM10, U6, CDB6, MHB6, JD1 (A$^2$type) of Phytophthora infestans isolated in Korea and F8l7, DNC303 (A$^1$type), IB908, DN107 (A$^2$type) obtained from Japan were investigated. Mycelium of P. infestans grew better on V-8 juice agar and rye meal agar than on the other media. Oospores were produced most abundantly on V-8 juice agar. Mycelium extended more 16.6, 8.3, and 5.2% on V-8 juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively, and oospores are produced 76.0, 58.0, and 34.6 % on V-8juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively. Oospores more produced on detached potato plant disks when $A^1$ and $A^2$ type exist simultaneously which indicating that variation of population can occur in the field, but the rate of oospore formation and the number of oospores produced was low and small quantity.

• Korean Journal of Weed Science
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• v.15 no.1
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• pp.73-84
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• 1995

Four malformin A's produced by Aspergillus niger van Tiegh. were separated by HPLC equipped with $C_{18}$ reversed-phase column and subjected to structural determination. Amino acid analyses and mass spectra data of the compounds indicate that they structurally resemble the cyclic pentapeptide malformin $A_1$. Their structures were deduced by two dimensional NMR and MS/MS experiments as cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Ile for $A_1$, cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Val for $A_2$, cyclo-D-Cys-D-Cys-L-Val-D-Leu-L-Leu for $A_3$, and cyclo-D-Cys-D-Cys-L-Val-D-Ile-L-Val for $A_4$. Among the mal-formin A's, the structure of $A_3$ was identical to that of malformin C, which was produced by A. niger strain AN-1. All the malformin A's caused severe curvatures of corn(Zea mays L.) roots and the activities of the malformin A's with molecular weight 529 were greater than those with molecular weight 515. Malformin $A_1$ caused the corn root curvature by 83% at a concentration of $0.25{\mu}M$. In the mung bean(Phaseolus aureus Roxb.) hypercotyl segment test, however, the molecular weight of malformin A's was not a factor influencing the physiological activities. Malformin $A_1$ stimulated the growth of mung bean hypercotyles by 165% at a $0.1{\mu}M$ concentration.

• Childhood Kidney Diseases
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• v.3 no.1
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• pp.20-26
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• 1999

Purpose : Several modulatory factors for renal peripheral benzodiazepine receptor (PBR) has been reported, but their physiological significance remains elusive. Tissue-specific, stress-induced down-regulation of renal PBR coupled with the pharmacological stimulation of these effects by angiotensin II suggested that physiological significance of renal PBR may be related to the pathophysiology of stress-induced hypertension. The boderline hypertensive rat (BHR) has been used extensively to study the interaction of environmental factors, such as stress and blood pressure. The BHR is the first-generation progeny of a cross between the spontaneously hypertensive rat and the control Wistar-Kyoto rat. The pathogenesis of stress induced hypertension in this model is not demonstrated well. Methods In this study, BHR (male, 150-200 g) and Sprague-Dawley (SD, male, 150-200 g) rats were treated by repeated immobilization to induce anxiety. We used plus-maze performance to observe the level of anxiety by measuring percent open crosses and percent time in open. Results : Percent open crosses and percent time in open in BHR were lower than in SD rats (P<0.05). Receptor densities of renal PBR in BHRs were significantly lower than those of SDs (P<0.05). We also observed that the renal PBR was upregulated in the repeatedly stressed (immobilization, 2 hours daily, for 2 weeks) rats, both in the BHR and SD. However, the density of renal PBR in the stressed BHR was still lower than that of stressed SD. Renal PBR has been suggested to be an important organs which Is responsible for the production of cholesterol-derived products during stress. Conrlusion : From these results, it can be summarized that the lowed density of renal PBR may be involved in the pathogeneis of stress-induced hypertension.

• The Korean Journal of Physiology
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• v.7 no.2
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• pp.49-58
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• 1973

The distensibility of the major arteries has been investigated extensively, but the value expressed as Young Modulus varies widely by the different schools of the investigators, the major reason undoubtedly being the difficulties encountered in the measurement. In the present study, an attempt was made to elucidate the distensibility of the external carotid artery of the rabbit, which was placed in saline immediately after removing from the apparently healthy, normal rabbit without anesthesia. The circular section strip and longitudinal section strip were made from the whole artery, and Young Modulus of the whole artery, circular section and longitudinal section strips was calculated from the length-tension curve of each sample. Also, the similar samples of the artery seperately obtained were placed in ATP solution in the concentration of 0.15 mM and 0.30 mM, and Young Modulus was similary calculated. Experiments were performed at 15,45 and 75 min after the artery was removed from the rabbit, and the results thus obtained are summarized as follows. 1) Young Modulus of the whole external carotid artery of the rabbit in saline was $4.74{\times}10^7dyne/cm^2$ at 15 min, but lower values were obtained at 45 and 75 min, Young Modulus being $4.62{\times}10^7dyne/cm^2\;and\;4.13{\times}10^7dyne/cm^2$, respectively. When the arterial samples were placed in ATP solutions, Young Modulus did not change much throughout the experiment, and lower Young Moduli were obtained in 0.30 mM ATP solution than in 0. 15 mM ATP solution. 2) Young Modulus Of the Circular Section Strip in Saline was $4.11{\times}10^7dyne/Cm^2,\;3.75{\times}10^7dyne/cm^2\;and\;3.90{\times}10^7dyne/cm^2,$ respectively, at 15, 45 and 75 min, the value at 15 min being the highest. However, when the strip was placed in ATP solutions, no appreciable change was observed throughout the experiment, and Young Moduli were lower in 0.30 mM ATP solution than in 0.15 mM ATP solution. 3) Young Modulus of the longitudinal section strip in saline was $2.12{\times}10^7dyne/cm^2,\;2.48{\times}10^7dyne/cm^2\;and\;2.46{\times}10^7dyne/cm^2$, respectively, at 15, 45 and 75 min, Young Modulus being slightly elevated in the latter part of the experiment. A similar tendency was observed when the strip was placed in ATP solutions.

• Korean Journal of Ecology and Environment
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• v.43 no.3
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• pp.409-417
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• 2010

Present study aimed to investigate morphological and physiological change in the tissues of Carassius auratus after exposure against Pb (lead) with various rearing condition. We measured the level of accumulated heavy metal and analyzed ultrastructure with transmission electron microscopy. The heavy metal, Pb, was accumulated in the gill, bone and integument increased drastically for exposure periods, the 40 days. The accumulation of Pb in the gill showed relatively higher than that in other tissues. And accumulated Pb amounts also were increased with exposure time dependent manner in the gill, bone and integument tissues. Also, specific activities of antioxidation enzymes in all tissue after exposure to Pb were increased in the course of exposure. And the activities of SOD from tissues exposed to Pb were increased 2 folds than those from the unexposed but GPX activities were maintained constant. The increased numbers of mucous cells in gill tissues exposed to Pb were determined and morphological changes, such as clubbing and fusion, were shown secondary lamella. Also, exposure of Pb for 40 days on gills tissues cause membrane damage in mitochondria and nucleus. In kidney tissues, the atrophied glomerulus was observed, and the empty space in Bowman's capsule was expanded. Based on the all results, it is suggested that the exposure to the high level of Pb for long period affect on the morphology of tissues, and change the enzymatic balance in C. auratus.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.495-501
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• 1993

This experiment was carried out to find out the dormancy physiology, method of softening culture and evaluation of nutritional value of wild garlic, Ulrung-native Allium victorialis var. platyphyllum. In March, a new bulbs, the shoot and bulbs began to develop until the bulbs showed their complete dormant states in late August. The bulbs renewed to another one in every years. When shoots germinated about $1{\sim}2\;cm$ from mother bulbs, the soft tissues in the mother bublbs was degenerated and finally remained as only fiberous tissues unlike the other bulbaceous plants. There was a high inhibiting activities like ABA in the bulbs. This is believed that this inhibiting substance like ABA in the bulbs is related to the dormancy of wild garlic. Although the immatured bulbs, harvested at May and June, was treated with chilling for 90 days, it didn't germinate their shooting, but the matured bulbs, harvested at July and August, could germinate their shooting over 1 cm in 75 and 60 days chilling treatment, respectively. The shoot elongation was promoted by the longer chilling periods, the later harvesting day and the dark condition. The crude fiber content of leaf and stem increased at more expanded leaf and higher light intensity condition. Since the shoots, grown from germinating to leaf expanding time, had a good quality for food stuff and had less crude fibers, we supposed this period is to be most appropriate for harvesting time.