Childhood Kidney Diseases
- Volume 3 Issue 1
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- Pages.20-26
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- 1999
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- 2384-0242(pISSN)
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- 2384-0250(eISSN)
Effects of Repeated Immobilization Stress on the Renal Peripheral Benzodiazepine Receptor in Rats
반복적 부동화 스트레스가 흰쥐 신장의 말초성 benzodiazepine 수용체에 미치는 영향
- Park Yong Hoon (Department of Pediatrics and College of Medicine, Yeungnam University) ;
- Moon Han Ku (Department of Pharmacology and College of Medicine, Yeungnam University) ;
- Shin Son Moon (Department of Pharmacology and College of Medicine, Yeungnam University) ;
- Lee Eun Ju (Department of Pharmacology and College of Medicine, Yeungnam University) ;
- Lee Eun Sil (Department of Pharmacology and College of Medicine, Yeungnam University) ;
- Ha Jeoung-Hee (Department of Pharmacology and College of Medicine, Yeungnam University)
- 박용훈 (영남대학교 의과대학 소아과학교실) ;
- 문한구 (영남대학교 의과대학 약리학교실) ;
- 신손문 (영남대학교 의과대학 약리학교실) ;
- 이은주 (영남대학교 의과대학 약리학교실) ;
- 이은실 (영남대학교 의과대학 약리학교실) ;
- 하정희 (영남대학교 의과대학 약리학교실)
- Published : 1999.05.01
Abstract
Purpose : Several modulatory factors for renal peripheral benzodiazepine receptor (PBR) has been reported, but their physiological significance remains elusive. Tissue-specific, stress-induced down-regulation of renal PBR coupled with the pharmacological stimulation of these effects by angiotensin II suggested that physiological significance of renal PBR may be related to the pathophysiology of stress-induced hypertension. The boderline hypertensive rat (BHR) has been used extensively to study the interaction of environmental factors, such as stress and blood pressure. The BHR is the first-generation progeny of a cross between the spontaneously hypertensive rat and the control Wistar-Kyoto rat. The pathogenesis of stress induced hypertension in this model is not demonstrated well. Methods In this study, BHR (male, 150-200 g) and Sprague-Dawley (SD, male, 150-200 g) rats were treated by repeated immobilization to induce anxiety. We used plus-maze performance to observe the level of anxiety by measuring percent open crosses and percent time in open. Results : Percent open crosses and percent time in open in BHR were lower than in SD rats (P<0.05). Receptor densities of renal PBR in BHRs were significantly lower than those of SDs (P<0.05). We also observed that the renal PBR was upregulated in the repeatedly stressed (immobilization, 2 hours daily, for 2 weeks) rats, both in the BHR and SD. However, the density of renal PBR in the stressed BHR was still lower than that of stressed SD. Renal PBR has been suggested to be an important organs which Is responsible for the production of cholesterol-derived products during stress. Conrlusion : From these results, it can be summarized that the lowed density of renal PBR may be involved in the pathogeneis of stress-induced hypertension.
목 적 : 스트레스 유발 고혈압을 일으키는데 말초성 benzodiazepine수용체가 중요한 역할을 하리라 추정되어 왔다. 반복적 부동화 스트레스에 의한 신장의 말초성 benzodiazepine수용체의 변화 양상을 Sprague-Dawley rats와 boderline hypertensive rats의 두 실험동물군에서 비교, 관찰하여 고혈압을 유발하는데 신장의 말초성 benzodiazepine 수용체의 병태생리학적 기능을 규명하고자 하였다. Benzodiazepine수용체의 변화 양상은 방사성 동위원소를 사용한 수용체 결합 반응으로 검색하였으며 elevated plus maze검사로 각 실험동물의 불안도를 측정하여 각 군간의 결과를 비교, 관찰하였다. 방 법 : 불안도를 보기 위하여 측정한 plus-maze performance에서 percent open crosses는 Sprague-Dawley rats (