• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.534-537
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• 2002

An agarase gene from Bacillus cereus ASK202 was expressed in high levels by E. coli BL21(DE3)/pEBA1 using pET28a(+) vector system with the inducible T7 promoter in the presence of isopropyl- ${\beta}$ -thiogalactopyranoside. The open reading frame encodes 761 amino acid residues with a calculated molecular weight of 83,300 daltons and a potential signal peptide about 36 amino acid residues at the N-terminus. E. coli BL21(DE3)/pEBA1 produce 1280 unit/ ${\ell}$ of agarase. The optimum physical condition for the agarase activity was pH 5.6, and $40^{\circ}C$, respectively. The agarase activity was stable up pH $4.0{\sim}9.0$ and $4{\sim}40^{\circ}C$. The km and maximum rate of metabolism for agar were 0.068mg/$m{\ell}$ and 0.094mg/$m{\ell}{\cdot}min$, respectively.

• Korean Chemical Engineering Research
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• v.44 no.1
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• pp.81-86
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• 2006

This study was carried out to investigate the antimicrobial and antioxidant activities for applications of food additives and medicine using Rumex crispus which widely grown in domestic area. Antimicrobial activities of extract fractions were observed in all microbial species except for Pseudomonas areuginosa. Especially, Vibrio vulificus and Saccharomyces cerevisiae presented obvious antimicrobial activity. Antioxidant activities of the fractions of R. crispus extract were determined by DPPH radical scavenger activity. The fraction of ethyl acetate was presented similar antioxidant activities compared with that of BHA and ascorbic acid. Also, ethyl acetate fraction contained higher phenolic compounds than that of others. The antioxidant activity in the fractions of R. crispus extract was closely related with the content of phenolic compounds in that.

• Korean Journal of Medicinal Crop Science
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• v.11 no.2
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• pp.127-134
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• 2003

One hundred sixty species among Korean medicinal plants were tested on their antioxidative potentials. Antioxidants are useful materials which could be expected into development as food preservatives, health foods, cosmetics and drugs. Korean peninsula have many potential in antioxidant source which includes various medicinal herbs. It grounded on the fact that Korean medicinal plants have been used as folk therapy for long time and still do in oriental medicine. From the study, effective free radical scavengers compared with ${\alpha}-tocopherol\;of\;13.5{\mu}g/ml\;in\;RC_{50}$ were Geranium sibiricum, Geum japonicum, Geranium nepalense subsp. thunbergii and Paulownia coreana which showed $19.3{\mu}g/ml,\;22.5{\mu}g/ml,\;23.9{\mu}g/ml,\;and\;27.2{\mu}g/ml$, respectively, Acer mono and 38 plants showed strong potential in inhibition rate on linoleic acid oxidation (above 90%). In conclusion, we expect that the selected medicinal plants must be more studied as antioxidant and then developed as many industrial materials.

• Korean Journal of Environmental Agriculture
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• v.26 no.4
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• pp.337-342
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• 2007

To obtain the data on the risk assessment of residue levels of organophosphorus and organochlorine pesticides in fresh ginseng and red ginseng extract, the residual pesticides in samples are surveyed with GC-NPD and GC-ECD for quantitative analysis and GC-MSD for qualitative analysis. The residual organophosphorus pesticides, such as diazinon, pyrimethanil, tolclofos-methyl, metalaxyl, diethofencarb, parathion, cyprodinil, tolylfluanid and kresoxim-methyl, are not detected in fresh ginseng from Punggi. The residual organophosphorus pesticides except tolclofos-methyl are not detected in fresh ginseng from Sangju. Average amount of tolclofos-methyl in fresh ginseng from Sangju are $0.054{\pm}0.008\;mg/kg$, representing $18{\pm}2%$ of MRL of 0.03 mg/kg on fresh ginseng in Korea. The residual organochlorine pesticides, such as BHC isomer, DDT isomer, aldrin, azoxystrobin, captan, cypermethrin, deltamethrin, dieldrin, difenoconazole, endosulfan-sulfate, endrin, fenhexamid, quintozene, ${\alpha}$-endosulfan and ${\beta}$-endosulfan, are not detected in fresh ginseng from Punggi and Sangju. The residual organophosphorus and organochlorine pesticides in red ginseng extract from Punggi and Sangju are not detected.

• Analytical Science and Technology
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• v.18 no.3
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• pp.257-262
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• 2005

Mitochondrial DNA (mt DNA) sequence analysis has been a useful tool for species identification of animals and human individuals. Two hypervariable regions (HV1 and HV2) in control region of mitochondrial genome were analyzed for human individual identification. In case of animal species identification, several genes on mt DNA such as cytochrome b (cytb), RNAs, cytochrome oxidases (CO) were used. In this study, co-amplification of HV1 and cytb was carried out in order to check the contamination of animal DNA and to verify the human DNA. The primer sets used in PCR were H15997/L16236 for HV1 and H14724/L15149 for cytb. PCR products for HV1 and cytb were 239 bp and 425 bp, respectively. The appearance of two bands on agarose gel implied the DNA came from human, however the single band of cytb gene represented the non-human animal DNA.

• YAKHAK HOEJI
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• v.53 no.6
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• pp.334-341
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• 2009

Aging society and aged society are determined by the component ratio of the elderly to the whole population; aging society is defined as a society whose ratio of the elderly population is less than 7%, and it is called aged society when the ratio is over 14%. Korea's aging process is so fast and unprecedented over the world according to many statistical data and investigations. The purpose of this study was to provide guidance and basic data to educate the elderly for the proper use of medication and health functional food. This study was carried out on 80 subjects of age 65 or older in Cheong Jusi in Choogncheongbuk-do. They answered questionnaire that was prepared for this study. The data were collected from August 4, 2008 to August 18, 2008. The collected data were analyzed by using the SPSS 12.0OK (for window) program and Microsoft EXCEL. The following is a summary of the results of this study. First, in the aspect of purchasing, the majority of respondents bought OTC drugs and health functional food for health and illness prevention. Purchasing places of drugs were diverse. Second, in the aspect of taking drugs, the older answerers were, the higher the number of drugs being taken was. Third, in the aspect of proper storage of drugs, most respondents have no specific spaces. Finally, regarding recognition of drug abuse and disposal, most people have little awareness or have no idea.

• Journal of Plant Biology
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• v.35 no.1
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• pp.1-8
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• 1992

The characteristic of Cyanophyte genus SG63 is similar to that of Aphanot hece sp. The optimal growth was found with the concentration of NaCI and $MgSO_4{\cdot}7H_2O$ on the culture medium. The most optimal condition is 56%0 of NaCl (S4 medium) and 20%0 of $MgSO_4{\cdot}7H_2O$ (M2 medium). The synthesis of chlorophyll a, phycocyanin and soluble proteins is affected by the concentration of the two mineral elements in culture. Especially, the content of chlorophyll a and phycocyanin decreases on the most highly saline medium. The identified principal carotenoids are ${\beta}-carotene$, echinenone, zeaxanthin and myxoxanthophyll. The rates of concentration of protein/chlorophyll a and phycocyanin/chlorophyll a are low on the S4 medium. Inversely, these rates are the highest on the M2 medium. Accordingly, the high concentration of $MgSO_4{\cdot}7H_2O$ provoke the synthesis of phycocyanin and total proteins.oteins.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.29 no.2 s.43
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• pp.251-270
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• 2003

Cosmetics have had only simple efficacy such as cleansing, beautification, and so on. The latest development tendency for cosmetics turned to make goods that is effective and efficacious for whitening, anti-wrinkle, etc., due to various wants of consumers, development of relative technical, etc. According to this stream, Korea Cosmetics Law has been enacted by the Notification of the Korea Food and Drug Administration in 2000. The establishment of Korea Cosmetics industry and opened regular 'Cosmeceuticals Time'. Since the Korea Cosmetics Law took effect on July 2000, the cosmeceuticals system has settled down according to its lights. This research studied how women consumers were understanding the cosmeceuticals system focused on their life style. This study is considered to offer a basic information about consumer's type and to be a groundwork data for working out a marketing strategy about the subdivision of cosmeceuticals market.

• Journal of the East Asian Society of Dietary Life
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• v.9 no.2
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• pp.200-206
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• 1999

This study was carried out to get a industrial information about a possibility of IgY antibody production, antimicrobial activity and Properties of IgY antibody in egg yolk. After the initial immunization the anti-Salmonella typhimurium IgY antibody level gradually were decreased from firth week to tenth week. On the other hand, the antibody level in the serum were increased from the first week, reaching its peak in the sixth week. Molecular weights of IgY were estimated approximately 72-75KD in a heavy chain and 30-40KD in a light chain by electrophoresis.

• Journal of the East Asian Society of Dietary Life
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• v.9 no.2
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• pp.207-213
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• 1999

This study was carried out to get a industrial information about a possibility of IgY antibody production, antimicrobial activity and properties of IgY antibody in egg yolk. The residual antibody activities of IgY were 91.5% after heating for 30min at 6$0^{\circ}C$. At the same time. these activities, were 73.2% after heating 15min at $65^{\circ}C$ and decreased vapidly at 7$0^{\circ}C$ and little antibody activity was left after heating for 15min at 8$0^{\circ}C$. When the prepared IgY was incubated at various pH ranges from 7 to 2 for 5hr at 37$^{\circ}C$., the antibody activity was stable from pH 7 to 4 and remained to 69.8% at pH 3.