• Korean Journal of Plant Resources
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• v.10 no.1
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• pp.50-57
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• 1997

Using a bioassay for tuber enlargement activity, which was carried out with culture of microtuber of nodal stem segments in vitro, the endogenous substances was detected from leaves of yam(Diosorea alata L., cv. Solo Yam) treated under the short and the long day length condition. Tuber enlargement activity was found in both the aqueous and ethyl acetate phase of extract obtained from leaves of yams. Those activity was found in both the aqueous and ethyl acetate phase of extract obtained from leaves of yams. Those activities increased under the short day length, whereas not increased under the long day length. Guided by bioassay, the active substances in the ethyl acetate phase were purified by charcoal adsorption chromatography. The result obtained indicated that the 40% ethanol fraction contained the most prominent tuber enlargement activity. In seasonal changes of the leaves, tuber enlargement activity remained almost constant during summer(June-August), but after. the middle of September, the activity increased gradually and then reached a maximum in early October. Jasmonic acid (JA) was isolated with the guidance of microtuber test and identified by gas chromatography. The level of endogenous JA in the leaves of plants was more than $290{\mu}g/kg$ at the 10 hr day length. These results seem evidences for the occurrence of the tuber enlargement activity which is formed in leaves under the short days and transmitted to under ground part to induce growth and enlargement of tuber.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.97-97
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• 2018

본 연구는 오미자 유래의 적색소를 추출하는데 있어 최적의 경제성과 생리활성을 나타내는 추출공정을 확립하고자 실시하였다. 국내산 오미자 건조물을 증류수와 함께 1시간, 3시간, 8시간, 16시간, 24시간 동안 $4^{\circ}C$, $15^{\circ}C$ 및 $60^{\circ}C$에서 적색소를 추출하였으며 각 추출조건의 추출물을 농축하여 수분을 제거한 뒤 무게와 OD값을 측정하였다. 농축물의 무게는 16시간 $15^{\circ}C$, 24시간 $15^{\circ}C$, 16시간 $60^{\circ}C$ 추출조건 순으로 높게 나타났으며 OD값은 16시간 $60^{\circ}C$에서 가장 높은 값을 나타내었다. 이들 추출물을 마우스 유래 adipocyte (3T3-L1)에 처리하여 5일간 배양한 후 세포내 지방 축적 억제 정도를 실험한 결과 세포내 중성지방은 모든 추출물 처리군에서 control과 비교시 유의적으로 억제되었다. 특히 추출온도와 관계없이 8시간 이상 추출한 모든군에서 control군보다 20% 이상 감소하였다. C57BL/6 생쥐를 이용하여 고지방식이로 비만을 유도하면서 오미자 추출물을 경구투여 한 뒤 체중증가를 조사한 결과 고지방식이군과 비교하여 유의적으로 체중이 감소함을 보였다. 따라서 오미자 유래 적색소를 추출하기 위한 추출공정에서 용매로 물을 사용할 경우 추출시간은 최소 8시간 이상, 추출온도는 실온 또는 $60^{\circ}C$에서 실시하는 것이 최적 추출 공정 조건으로 사료된다.

• Food Science and Preservation
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• v.16 no.2
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• pp.259-265
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• 2009

To develop an effective anti-hangover product, hot-water extracts of 25 medicinal herbs were screened for inhibition or activation of alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase(ALDH), and 12 herbs were selected for further study. Chosen medicinal herb extracts(CMHEs) were fermented by Lactobacillus delbruechii subspecies lactis for 10 days at $35^{\circ}C$ after saccharification with nuruk(malt inoculated by 5 types of microbs) for 72 hours at $35^{\circ}C$ and both CMHEs and fermented CMHEs(FCMHEs) were explored for anti-hangover effects in vitro. We found significant ADH inhibition by hot-water extracts of Pueraria thunbergiana, Hovenia dulcis Thunb, Lycium chinense, Glycyrrhiza uralensis, Acanthopanax sessiliflorus, Liriope platyphylla, and Ixeris dentata, and significant ALDH activation by extracts of Acanthopanax sessiliflorus, Lycium chinense, Ixeris dentata, and Polypori umbellati of the Polyporaceae. The ADH effects on CMHE and FCMHE were -20.22% and -62.63% of control values, and the ALDH effects 173.20% and 280.17%, respectively. In rats given 20%(v/v) alcohol(15 mL/kg), FCMHEs significantly decreased blood acetaldehyde concentrations on 3 hours after ethanol administration, in a dose-dependent manner(p<0.05). Notably, blood acetaldehyde concentrations were markedly reduced in animals given FCMHEs(400 mg/kg) compared to levels seen in rats receiving CADB(commercial alcohol detoxification beverage). Thus, anti-hangover effects were promoted by fermentation of certain medicinal herb extracts.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.2
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• pp.173-180
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• 2015

In the present study, antioxidant activities and tyrosinase inhibition of Perilla frutescens, Houttuynia cordata and Camellia sinensis extracts and the extract mixtures (PHC) were investigated. PHC showed 80.2% and 98.0% of free radical scavenging activity in DPPH and ABTS analysis, respectively, and 50% tyrosinase inhibition in $1000{\mu}g/mL$ concentration. HaCaT cells did not show cell toxicity in $100{\mu}g/mL$ of the PHC. Furthermore, HaCaT cell viability by co-culture with extract H. cordata was increased more than 10% compared with untreated cells. However, the cell viability was decreased in $500{\mu}g/mL$ of the extract C. sinensis and the PHC. These results suggested that about $100{\mu}g/mL$ concentration of the PHC showed proper tyrosinase inhibitory effect and antioxidant activities. The PHC could be used as multifunctional cosmeceutical agents.

• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.24-30
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• 2003

Aqueous extracts from eleven species of Korean medicinal plants were screened for antiviral activity against transmissble gastroenteritis virus (TGEV) and porcine epidemic diarrhae virus (PEDV). Seven of eleven plant extracts were found to have cytotoxicity for ST cells at the concentration of 1mg/ml, and ten extracts except Ephedra sinica were found to have non-cytotoxicity for Vero cells at the same concentration, Extracts of Zanthoxylum piperium, Cudrina tricuspidata, Clerodendron trichomum, Sophora flavescens, effectively inhibited TGEV, respectively. There were the antiviral activity against TGEV in the extracts of Glesdistsia sinensis, Euonymus alatus, Ficus carica, Solanum nigrum, Ephedra sinica, Xanthium strumarium, Acanthopanax seeliflorus in spite of their cytotoxicities. Nine extracts except Xanthium strumarium and Acanthopanax seeliflorus were found to have the antiviral activitity against PEDV. Extracts of Zanthoxylum piperium, Sophora flavescens was shown to have antiviral activities against both TGEV and PEDV.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.84-84
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• 2018

인공재배 매실나무겨우살이의 의약 화장품의 소재 및 산업화 가능성을 검토하기 위해 80% 에탄올 및 증류수 초음파추출물의 Tyrosinase 저해활성을 측정하였다. 인공재배한 매실나무겨우살이(PM, 동결건조) 및 자연산 굴참나무겨우살이(QM, 열풍건조, $30^{\circ}C$, 7day)의 80% 에탄올, 증류수 초음파추출물에 대한 tyrosinase 저해활성을 측정한 결과, 물 추출물의 경우 100 ppm의 농도에서 PM과 QM 모두 5분이 경과 하였을 때 PM은 88.37%, QM은 87.69%로 최대치를 나타내었고, 농도와 시간이 증가함에 따라 tyrosinase 저해활성이 감소하는 경향을 보여주었다. PM 80% 에탄올 추출물은 5분이 경과하였을 때 2,500ppm이 92.08%로 최대값을 보여주었고 이후 시간이 지남에 따라 저해활성이 감소되는 경향을 나타내었다. 반면 5,000ppm은 5분이 경과하였을 때 66.38%로 최소값을 나타낸 반면, 20분까지 저해활성이 증가하였고 10분이 경과한 이후로 거의 일정하게 유지되어 지속적으로 감소되는 1,000ppm보다 높은 저해효과를 보여 주었다. QM 80% 에탄올 추출물은 5분이 경과하였을 때 1,000ppm이 88.14%로 가장 높았고 5,000ppm은 63.49%로 가장 낮은 저해활성을 보여주었다. 15분까지 1,000ppm이 가장 높은 저해활성을 보여주었으나 꾸준히 감소한 반면, 2,500ppm의 경우 시간이 경과함에 따라 비교적 일정한 저해활성을 보여주어 15분이 경과한 이후로 2,500ppm의 저해활성이 가장 높게 나타났다. 25분이 경과하였을 때 2,500ppm은 70.27%로 가장 높은 저해활성을 나타내었고, 5,000ppm은 30.09%로 가장 낮은 저해활성을 보여주었다. 본 연구 결과에 따르면, 물 추출물의 경우 겨우살이의 농도가 낮을수록 더 높은 tyrosinase 저해활성을 나타내었고, 80% 에탄올 추출물의 경우 2,500ppm에서 가장 높은 저해활성을 나타내어 의약 화장품의 소재 및 산업화 가능성이 높음을 확인할 수 있었다.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.537-548
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• 2016

The objective of this study was to evaluate the antimicrobial activities of 9 kinds of medicinal plants against crown gall in grapevine. The medicinal plants extracted with several solvent systems were screened for in vitro antibacterial activity by the disc diffusion method. The ethanol and ethyl acetate extracts from magic lily flowers, tachys roots, asian plantain flowers and seeds, sweet wormwood leaves, stems and flowers, immature bitter melon fruits, cockscomb flowers, and peach tree resin showed in vitro antimicrobial activities against Rhizobium vitis with growth inhibition zones ranging from 10 to 27 mm in diameter. The minimum inhibitory concentration values of extracts against R.vitis ranged from 10,000 in Asian plantain flower and 50,000 fold diluted extracts in sweet wormwood flowers, stems, leaves, cockscomb leaves and immature bitter melon fruits. The active fractions of ethyl acetate and ethanol extracts from the medicinal plants were partially separated through silica gel column chromatography and thin layer chromatography (TLC). The active fractions were separated at Rf 0.36, 0.69, 0.75, 0.84, and 0.94 in sweet wormwood extracts, Rf 0.96 and 0.99 in cockscomb flower extracts, Rf 0.92 and 0.97 in cockscomb leaf extracts, and Rf 0.85 in immature bitter melon fruit extracts in TLC analysis developed with hexane:ethyl acetate (20:80, v/v) and methanol:chloroform (20:80, v/v). Among extracts from plants with in vitro antimicrobial activities, sweet wormwood, cockscomb leaves, and immature bitter melon fruits showed in vivo antimicrobial activities with inhibition activity of 100, 67, and 83.3%, respectively, in 'Kyoho' grapevine inoculated with R. vitis compared with the untreated control. These findings indicate that extracts of medicinal plants could be used as sustainable candidates to control crown gall disease caused by R. vitis in grapevines.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.78-78
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• 2002

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.82-82
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• 2001

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.161-161
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• 2005