• Journal of Practical Agriculture & Fisheries Research
• /
• v.23 no.1
• /
• pp.5-12
• /
• 2021

In order to extract the essential oil contained in the Elsholtzia splendense Nakai, a fully-bloomed individual was collected and the living body was used for the experiment. Plants were divided into 0.7kg of flowers and 1.5kg of leaves + stems, and extracted by parts and used for scent pattern analysis, and extracted after adding 5kg of outpost for the analysis of essential oil components. Essential oil extraction was performed using the SDE method improved by Schultz et al. (1977), and the extraction time was limited to 1 hour after the material started boiling. The extracted essential oil component was analyzed for fragrance pattern analysis using an e-nose, and the results of analyzing the substance of the essential oil component by GC-MS are as follows. 1. As for the fragrance pattern analysis, in the case of essential oils extracted from flowers, the scent quality was the best at 10-20 minutes, and the scents extracted from stems and leaves were somewhat of poor scent quality, but the fragrance was good at 10-40 minutes. The intensity of scent was the strongest in 10-20 minutes, and the intensity of incense was high even in 30-40 minutes. The scent extracted from the stems and leaves was generally not strong, but appeared high in 10 to 20 minutes. 2. There were 40 kinds of essential oils contained in Elsholtzia splendense Nakai oil. Among them, Mequinol, Benzene, 1,2,3,4-tetramethyl, Elsholtziaketone, and Dehydroelsholtziaketone were identified.

• Korean Journal of Medicinal Crop Science
• /
• v.6 no.2
• /
• pp.149-153
• /
• 1998

This experiment was conducted to increase essential oil and aromatic contents on roots of Codonopsis lanceolata Trautv with different shading treatments. Vine length was prolonged by shading treatment compared with non-shading. Fresh root weight in 75% shading treatment was highest with 13g per plant. Crude components such as crude protein, fat and fiber contents decreased, while crude ash increased as shading degree increased. Forty-eight volatile aromatic compounds in the root were identified by GC/MS. Major aromatic compounds were 1-hexanol, cis-3-hexanol, and trans-2-hexanol. In particular, trans-2-hexanol was highest in the 75% shading treatment attaining the 160.32 % area. Conclusively, it was suggested that the 75% shading treatment was to some extent effective to improve the content of essential oil and aromatics in the roots of Codonopsis lanceolata Trautv.

• Korean Journal of Medicinal Crop Science
• /
• v.6 no.4
• /
• pp.299-304
• /
• 1998

This experiment was conducted to determine the essential oil content and the aromatic constituents in the leaves of Saururus chinensis Baill, Angelica dahurica Fischer and Cnidium officinale Makino. Volatile aromatic compounds in three aromatic medicinal plants were extracted with steam distillation extraction method and identified by GC/MS. Major aromatic compounds in Saururus chinensis Baill were 1,6-octadien-3-ol, 1, 3-benzodioxole, myristicin, ${\alpha}-cadinol$ and patchouene. Major aromatic compounds in Angelica dahurica Fischer were terpinolene, 3-carene, ${\beta}-caryophyllene$, ${\beta}-cubebene$, butylated hydroxy toluene, caryophyllene oxide, piperonal, and in Cnidium officinale Makino were aristolene, benzocycloheptene, ylangene, valencene, ${\beta}-cedrene$, satene, and menthofuran. Essential oil content was highest in Saururus chinensis plant.

• Korean Journal of Plant Resources
• /
• v.30 no.1
• /
• pp.17-21
• /
• 2017

Since the acetylcholinesterase (AChE) inhibitor is used to treat Alzheimer's disease, the present study aimed to analyze the component with anti-AChE activity from the essential oil of Artemisia iwayomogi (Compositae). The four major components of the essential oil were identified to be camphor (29.8%), borneol (28.0%), eucalyptol (5.81%) and coumarin (5.49%) from a gas chromatography-mass spectrometry (GC-MS). The essential oil and its three components, camphor, borneol, and coumarin, were subjected to anti-AChE assay. The $IC_{50}$ values of the essential oil and coumarin were shown to be $0.298mg/m{\ell}$ and $0.236mg/m{\ell}$, though those of other two components, camphor and borneol, were more than $0.250mg/m{\ell}$. These results suggest that coumarin is an active substance of this essential oil with anti-AChE activity.

• Journal of the Korean Applied Science and Technology
• /
• v.38 no.1
• /
• pp.263-270
• /
• 2021

Allium Hookeri is a plant of the genus Allium, and prior research has been conducted on antioxidant, anti-inflammatory and antibacterial, but studies on essential oil extraction are insufficient. Therefore, in this study, GC-MSD was performed by extracting essential oils for Allium Hookeri leaves, roots, and whole parts. The cytotoxicity, antioxidant, and anti-inflammatory properties of Allium Hookeri leaves were confirmed to confirm their potential as a cosmetic material. The yield of essential oil extraction from Allium Hookeri leaves, roots and whole parts was found to be 0.01, 0.02, 0.01%. As a result of analyzing the aroma components of GC-MSD, the main components of essential oil of Allium Hookeri leaves were Diallyl trisulfide (34.02%) and Methyl allyl trisulfide (25.14%). At a concentration of 10%, where cytotoxicity was not confirmed, 39.69% inhibition of NO production and 88.26% of DPPH radical scavenging activity were shown. Through this, the Allium Hookeri essential oil presents the possibility of being useful as a raw material with antioxidant and anti-inflammatory effects in cosmetics.

• Korean Journal of Plant Resources
• /
• v.11 no.3
• /
• pp.279-282
• /
• 1998

This expriment was carried out to get basic information on composition and yield of aromatic constituents in leaves of four medicinal plants, Angelica tenuissima, Chrysanthemum zawadskii. ssp. latilobum, Artemisia iwayomogi and Artemisia capillaris. Volatile aromatic constituents, 28 compounds in Angelica tenuissima were identified and 19 compounds were indentified in Chrysanthemum zawadskii ssp. latilobum. Volatile aromatic constituents, 23 compounds in Artemisia iwayomogi and Artemisia capillaris were identified. Major volatile aromatic consitiuents analyzed by GC/MS in four plants were $\alpha$-pinene, camphene, sabinene, cis-2-hexanol, and camphor etc. Content of essential oils in Angelica tenuissima, Chrysanthemum zawadskii ssp. latilobum, Artemisia iwayomogi and Artemisia capillaris were 0.014, 0.275, 0.785, and 0.452%, respectively. As a result, it was suggested that a medicinal plant, Artemisia iwayomogi, was worthy of using as a useful material of perfume.

• Korean journal of applied entomology
• /
• v.56 no.4
• /
• pp.395-402
• /
• 2017

Overuse of synthetic pesticides caused negative side-effects such as environmental contamination, development of insect pests' resistance, and effects on non-target organisms. Plant origin substances without/or with low mammalian toxicity have been considered as promising alternatives to the synthetic pesticides. Fumigant toxicity of the essential oil of Iranian Yarrow, Achillea millefolium L., was investigated against a cosmopolitan stored-product insect pest: saw-toothed grain beetle (Oryzaephilus surinamensis L.). Chemical profile of this essential oil was studied by Gas Chromatography-Mass Spectrometry. Tested concentrations were significantly effective to the mortality of insect pest. A positive correlation between essential oil concentrations and pest mortality were realized. LC50 value (lethal concentration needed to 50% mortality) was achieved as $17.977(16.195{\pm}20.433){\mu}l/l$ air. The main components were 1,8-Cineole (13.17%), nerolidol (12.87%), ${\alpha}$-cubebene (12.35%), artemisia ketone (6.69%), ${\alpha}$-terpineol (5.27%), alloaromadendrene oxide (4.71%) and borneol (3.99%). Terpenic compounds including monoterpene hydrocarbons (8.19%), monoterpenoids (44.23%), sesquiterpene hydrocarbons (21.69%) and sesquiterpenoids (22.24%) were 96.35% of the total identified compounds. Results indicated that the terpene-rich A. millefolium essential oil may be considered as a safe bio-agent in the O. surinamensis management.

• Journal of the Korean Applied Science and Technology
• /
• v.31 no.3
• /
• pp.440-445
• /
• 2014

The purpose of this study was to investigate anti-inflammatory and antioxidant effects of essential oil from seed of Zanthoxylum schinifolium on cultured RAW 264.7 cell line. Antioxidant activity of essential oil was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. This essential oil was tested for cell viability on RAW 264.7 cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The effects of anti-inflammatory on LPS-induced RAW 264.7 cell line was studied by the content of nitric oxide (NO) and prostagladin $E_2$ ($PGE_2$) in cells. The essential oil of seed from Zanthoxylum schinifolium obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The essential oil showed low cytotoxicity as more than 98% cell viability in $40{\mu}g/mL^{-1}$ concentration. The essential oil of seed extracted from Zanthoxylum schinifolium presented obvious effect on inflammation. These results suggest that essential oil of seed from Zanthoxylum schinifolium may have value as the potential anti-inflammatory effects by decreasing the action of NO and $PGE_2$ and preventing the activation of oxidative.

• Korean Journal of Medicinal Crop Science
• /
• v.11 no.5
• /
• pp.364-370
• /
• 2003

Essential oil of Thuja occidental leaves was extracted by steam distillation method, and chemical composition of essential oil was analyzed by GC-MS. The essential oil was tested to antimicrorbiaI activity against 28 test microorganisms and antioxident assay through DPPH free radical scaveging test and insecticidal activity by mosquito insecticide assay. The major constituents in the essential oil of T. occidental were monoterpene as d-isothujone, ${\alpha}-thujone$, camphor, L-fenchone, and hornyl acetate. The essential oil have broad antimicrobiaI activity, which showed strong antimicrobiaI activity for Streptococcus pyogenes as bacteria, Alternaria alternata, Aspergillus niger, Aspergillus oryzae, Saccharomyces cerevisiae, and Streptococcus mutans as fungi. The essential oil also showed DPPH free radical scavenging ability at concentration of $36\;{\mu}{\ell}/m{\ell}$. In spraying and fumigation tests, the essential oil had strong insecticidal activity against mosquito.

• Food Science and Preservation
• /
• v.13 no.1
• /
• pp.108-114
• /
• 2006

This study was conducted to determine the volatile flavor compositions of the essential oil and the headspace of Capsella bursa-pastoris Medicus. Essential oil and headspace from the plant were extracted by simultaneous steam distillation extraction (SDE), and solid-phase microextraction(SPME) methods, respectively. Seventy-two compounds including 28 hydrocarbons, 4 aldehydes, 6 ketones, 16 alcohols, 4 esters, 8 acids, and 6 miscellaneous ones were identified in the leaf essential oil extracted by SDE method Sixty-eight compounds including 26 hydrocarbons, 2 aldehydes, 6 ketones, 17 alcohols, 4 esters, 6 acids, and 7 miscellaneous ones were identified in the root essential oil. According to the instrumental analyses the essential oil, phytol ($21.12\%$ in leaves, $20.94\%$ in roots) was the most abundant compound Alcohols, esters, and acids were main groups of the essential oil. On the other hand, thirty-eight compounds including 18 hydrocarbons, 3 aldehydes, 3 ketones, 9 alcohols, 2 esters, 3 miscellaneous ones were identified in the leaf headspace by SPME. In root headspace, thirty-three compounds including 16 hydrocarbons, 2 aldehydes, 1 ketone, 9 alcohols, 3 esten;, and 2 miscellaneous ones were identified. Hydrocarbons($44.02\%$ in leaves, $56.98\%$ in roots) were the main components of the headspace of Capsella bursa-pastoris Medicus.