• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.187-192
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• 2006

In order to investigate the effects of genetic variations of orchardgrass in tissue culture response, calli were induced from mature seeds of eight varieties, 'Hapsung 2', '93E', 'Amba', 'Ambassdor', 'Frode', 'Frontier', 'Potomac' and 'Roughrider', and plant regeneration frequency was compared. Significant differences were observed among the varieties in both callus induction and plant regeneration. Callus induction rate of viable seeds varied from 24.3% to 71.7%. Plant regeneration frequency ranged from 76.6% to 29.7%. 'Roughrider' varieties showed higher regenerability with the frequency of 76.6%. These results can be used not only to provide additional improvements in the plant regeneration frequency from transgenic callus, but also useful for molecular breeding of orchardgrass through genetic transformation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.615-625
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• 1997

An efficient protocol for plant regeneration from protoplasts of the indica rice variety IR43 has been developed. The procedure involved plating of embryogenic suspension-derived protoplasts on the surface of a filter membrane overlaying agarose-embedded feeder cells. Lolium multiflorum cell suspensions were preferable to these of Oryza ridleyi as feeder cells and Lolium suspensions supported colony formation from up to 0.68％ of the protoplasts, depending on the age of cell suspensions. Plant regeneration frequency was significantly improved by using maltose alone or in a 1:1(w/w) combination with sucrose as carbohydrate source and a simple dehydration treatment using a high concentration of agarose in the regeneration medium. Medium containing maltose or maltose mixed with sucrose increased the plant regeneration frequency compared with medium containing sucrose alone. The plant regeneration frequency was increased to 30.7 to 70.7％ following dehydration treatment, while the non-treated controls showed a regeneration frequency of 3.1 to 30.6％. Protoplast-derived plants were transferred to the glasshouse, flowered with morphologically normal.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.3
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• pp.265-270
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• 2004

Plant regeneration from seed-derived callus of Kentucky bluegrass(Poa pratensis L. cv. Kenblue) was investigated. Callus induced on the medium supplemented with 2 mg/L 2,4-D and 0.2 mg/L BAP showed highest frequency of plant regeneration on the regeneration medium supplemented with 1 mg/L NAA and 5 mg/L kinetin. Callus induced in the dark condition showed higher regenerability than that induced in the dim light. MS medium was better than N6 and B5 medium in enhancing plant regeneration. Maltose was superior to sucrose in plant regeneration as carbon source in the medium.

• Korean Journal of Medicinal Crop Science
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• v.14 no.1
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• pp.31-35
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• 2006

A protocol for plant regeneration from hairy root of Rehmannia glutinosa transformed by Agrobacterium rhizogenes ATCC15834 has been developed. Transgenic shoots were regenerated from hairy roots within 6 weeks after culture on the SH medium supplemented with 0.5 mg/l BA. Shoots were rooted on plant growth regulator free SH medium successfully. The transformed plants, which were regenerated from hairy roots, had thiner roots with extensive lateral branches, wrinkled leaves, shorter node, and grew faster compared with non-transformed plants. The biomass of the transformed plant was 1.28 g (F.W) per plant, significantly higher than the non-transformed plant (0.54 g F.W). The catalpol content in the transformed plant (0.56%) was also higher than that of the non-transformed plants (0.43%).

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.319-322
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• 1998

Culture conditions for high frequency embryogenesis and plant regeneration in anther cultures of various F$_1$ hybrid and homozygous lines of pepper (Capsicum annuum L.) are described. Anthers pigmented less than halfway from the distal end were dissected from the flower bud in which petals elongated 2 mm higher than the receptacle. They were placed on Dumas medium supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin. After four weeks of culture, embryos began to appear on anthers. After eight weeks of culture, frequencies of embryo formation reached up to 58.3%. Upon transfer to MS basal medium, greater than 95% of embryos developed into plantlets.

• Korean Journal of Plant Tissue Culture
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• v.21 no.5
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• pp.299-302
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• 1994

Immature zygotic embryos (up to 4mm in length) were cultured on MS medium supplemented with 0.5 to 8mg/L 2,4-D. Up to 87% of them formed somatic embryos on the plumule without producing an intervening callus. The site of somatic embryo formation was confirmed by culturing plumule explants, which consisted of shoot apical meristem domes with 1 or 2 leaf primordia excised from 2-week-old seedlings. When the concentration of 2,4-D was increased over 4 mg/L, the plumule explants produced nonembryogenic calli only, whereas the distal end of the cotyledons directly formed numerous somatic embryos at frequencies of up to 60%. Upon transfer onto MS basal medium,2 out of 15 somatic embryos converted into plantlets. The plantlets were potted to a soil mixture and grown to maturity in a phytotron.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.76-77
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• 2000

• Korean Journal of Plant Resources
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• v.11 no.1
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• pp.1-8
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• 1998

This study was conducted to establish mass propagation system from the axillary bud culture of chrysanthemum zawadskii H. which was used as material of medicinal plants. Shoot egeneration was better on MS medium with NAA and BA. The optimum concentraions of growth regulator for shoot regeneration differed depending on accessionsof C. Zawadskii. Shoot regeneration in Keungucheolcho was better on MS Medium with NAA 0.01mg/1 and BA 0.1mg/1 while Hyangrobonggucheocho was better with NAA 0.1mg/1and BA 0.3mg/1. Addition of NAA into medium was effective for induction of root from shoots regenerated. Shoot multiplcation was more effective when 10mg/1 spermine was added into medium than when other polyamines were treated ino medium . Randomly and specifically amplified polymorphic DAC banding patterns based on polymerase chain reaction (PCR) analysis were used to assess the genetic variation of plants regenerated from in vitro culture.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.48-48
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• 2023

지역의 역사성을 지니고 발전한 전통색은 지역 문화와 자연환경에 영향을 받는다. 전통색은 일반적으로 관용색명으로 표현하는데, 이는 세계인의 공용어가 아니므로 우리의 천연염색을 국내외 일반인과 공유하기 위해서는 표준화된 색명(KS-계열색, RGB, CMYK)을 사용하는 것이 필요하다. 우리나라에서 염료재로 활용된 염료식물은 393종으로 알려져 있다. 본 연구에서는 염료식물의 자원 활용과 그 보전적 가치를 알리고자 염료식물을 언급한 고문서 및 현대자료를 바탕으로 사용 부위, 매염제의 종류, 염색 시 발현 색상의 관용색명을 조사하였고, 이를 국가표준색상환에 대입하여 표준화에 대한 방향성을 제시하고자 한다. 연구 결과에 따르면, 염료식물 393종 중 관용색명을 가진 염료식물은 312종이었고, 관용색명의 정보가 없는 식물은 81종으로 나타났다. 염료식물의 사용 부위 정보를 가진 식물은 187종이었고, 이용부위는 식물체 전체(전초), 뿌리, 줄기(껍질), 줄기(심재), 나뭇가지, 잎, 열매(껍질), 꽃이었다. 염료식물에 활용되는 매염재는 15가지(천연 6종, 화학 9종)로 나타났다. 관용색명을 가진 염료식물 312종에서 조사된 540개 관용색명을 표준화 시키기 위하여 국가표준색상환에 대입한 결과, 표준화된 색명(KS계통색명, RGB, CMYK)을 갖는 식물은 127종, 관용색명은 있지만 표준화된 색명을 확인할 수 없는 식물은 185종인 것으로 나타났다. 향후 염료식물을 연구하는데 있어서 관용색명의 정보가 없는 염료식물에 대한 표준화된 색명을 찾아내는 연구가 이루어져야 할 것이다.

• 보존과학연구
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• s.6
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• pp.1-9
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• 1985