• Journal of the Korean Association of Geographic Information Studies
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• v.10 no.3
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• pp.40-48
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• 2007

We analyze relation between phytoplankton and marine environment based on data such as water temperature, phytoplankton, zooplankton, nutrient collected from the southern coast of Korea in the summer, 2004. The water temperature range of the study area was $20.5{\sim}31.5^{\circ}C$ and there was formed a water temperature frontal zone from $20.5^{\circ}C$ to $25.0^{\circ}C$ in Geojedo southern coast and Geomundo island. Especially, high density of nutrients were shown in the southern coast of Geojedo in which water temperature frontal zone was formed strongly, the concentration of chlorophyll-a which is appeared at the highest rate among the phytoplankton pigments was shown more than $0.4{\mu}g/L$ in the inside of frontal zone and zooplankton biomass was than $500mg/m^2$ in that area.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.6
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• pp.397-404
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• 1991

The steady state and decay characteristics of primary fluorescenece of phytoplanktons including Cyanophyceae and Cryptophyceae were investigated in vivo. At 580-640 nm region, fluorescence emission spectra were obtained from all algae examined. The observed fluorescence emission maxima were similiar$(\pm3\;nm)$ except Synechocorcus sp. (SYN). Considered $\lambda_{max}$ of emission spectra of phycobiliproteins and the excitation spectra with $\lambda_{max}=540-560nm$, it seems to be originated from biliproteins. Fluorescence lifetimes $(\tau)$ and decay curves were compared with standard solution of candidate organic compounds, b-phycoerythrin. The $\tau$ values obtained for phytoplankton with $\lambda_{max}=580nm$ were different depending upon the species of algae. The observed $\tau$ values were ranged from 1.39 ns to 1.95 ns. These are considerably shorter than $\tau(3.23\;us)$ for standard solution of b-phycoerythrin. The reduction of $\tau$ for phycoerythrin in vivo seems to be originated from effective energy transfer system between Chl. a and phycobiliprotein in intact cell. There are subtantial differences in fluorsecence spectra and lifetimes at the class level. At the species level, differences seems to be much smaller. The result of experiment suggests that measurement of fluorescence lifetimes may be helpful in the rapid characterization of algae. Direct application will likely be found in combination with the measurement of other luminescence parameters.

• Korean Journal of Ecology and Environment
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• v.52 no.2
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• pp.81-93
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• 2019

Phytoplankton is one of the important primary producers providing organic matter through photosynthesis in aquatic environments. In order to determine a temporal and spatial variation in primary productivity after weir construction in the Nakdong River, we investigated carbon uptake rates using in-situ $^{13}C$ labeling experiments and identified algal communities contributing to primary productivity using HPLC-CHEMTAX analysis from October to December, 2017. The primary productivity gradually decreased from fall to early winter season ($249{\sim}933mgC\;m^{-2}d^{-1}$ in October, $64{\sim}536mgC\;m^{-2}d^{-1}$ in November and $60{\sim}274mgC\;m^{-2}d^{-1}$ in December, respectively). This is attributed to the temporally declining light intensity and the decreasing biomass and physiological activity of phytoplankton in winter. The contribution of diatoms to the phytoplankton community in the Nakdong River was approximately 63% at all the sampling sites and seasons, while the contribution of cryptophytes increased from 9% in October to 32% in November and December. The temporal changes in the primary productivity and the dominant phytoplankton species in the mid and downstream weirs of the Nakdong River was investigated for the first time, after construction of the weirs, and major environmental factors controlling the temporal variation in primary productivity and phytoplankton communities were identified in this study. We suggest that seasonal field investigations will provide further information on the major environmental factors which affect the annual variation of primary productivity and phytoplankton communities.

• Korean Journal of Ecology and Environment
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• v.54 no.4
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• pp.303-314
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• 2021

To understand how to efficiently observe the biomass and community of phytoplankton, phytoplankton sampling was carried out from June to October 2019 at the Yeongju dam sediment control reservoir(YJ) and Bohyeonsan dam reservoir(BH1 and BH2). The results derived from microscopic observation, such as the conventional phytoplankton qualitative/quantitative analysis, and from the CHEMTAX method based on the pigments, were compared. The relative contribution of phytoplankton, calculated by the microscopy and CHEMTAX methods, showed a significant difference in all four classes: cryptophyta, chlorophyta, cyanobacteria, and diatoms. In addition, the correlation between the two observation methods was poor. This might be caused by methodological differences in microscopy that do not consider the varying cell sizes among phytoplankton species. In this study, by converting the cells into carbon, the slope between both carbon biomasses based on microscopy and CHEMTAX was improved close to the 1 : 1 line, and the y-intercept was closer to 0 for cryptophyta and diatoms. For cyanobacteria, the slope increased, the y-intercept decreased, and the plot approached 1 : 1 although the correlation coefficients were not improved in all classes. The present study suggests that application of CHEMTAX based on pigment analysis could be a possible approach to efficiently determine the relative carbon proportions of individual classes of phytoplankton community composition.

• Korean Journal of Ecology and Environment
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• v.45 no.2
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• pp.139-149
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• 2012

Four in situ incubation experiments were conducted in May, August and November 2009, as well as February 2010 to determine the seasonal primary productivity and the community structure of phytoplankton at the Seomjin estuary. The primary production of phytoplankton ranged from 9 to 3560 mgC $m^{-2}\;d^{-1}$. Primary productivity was the highest in the summer season (August), which was influenced by improved optical and temperature conditions of the water, as well as the supply of nutrients derived from its surrounding watershed. Particularly, the upper station (SJ-1, SJ-2) of Seom-jin estuary showed a higher productivity, as a result of inflow of input nutrients originated from the terrestrial source. The fucoxanthin, as an index pigment of diatoms showed the highest concentration (0.74~9.51 ${\mu}g\;L^{-1}$) at all stations, occupying 30~80% to total Chl a concentrations. The phytoplankton species composition determination, using a microscope showed similar results to the pigment analysis, which indicated diatom Skeletonema costatum, as the dominant species. The primary productivity in Seom-jin estuary indicates temporally and spatially large variation, according to different environmental conditions. Also, Skeletonema costatum has euryhaline features with relatively higher contribution.

• 한국해양학회지
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• v.20 no.2
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• pp.49-60
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• 1985

The interrelationships between distribution of phytoplankton and hydrographical conditions were studied from December 1983 to July 1984 in Yeongil Bay. The temporal variation of phytoplankton abundance seems to be mainly influenced by the vertical stability of water column and the freshwater run-off accompanied with possible nutrient input. The temporal progression of dominant species shows general succession pattern, especially skeletonema costatum was found a dominant species in each month. Although the differences between concentration of chlorophyll a and abundance of phytoplankton may be caused by cell size variation, during the study period they show felatively good linear manner. The phytohydrographic region based on multivariate analysis reflects the relationships between hydrographical condition and distribution pattern of phytoplankton.

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.499-506
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• 2013

To estimate the effect of algae to particulate organic matter in the upper regions of brackish Lake Sihwa, temporal and spatial variations of particulate organic carbon (POC) and phytoplankton pigments (chlorophyll a; Chl-a, pheophytin-a; Pheo-a), and their relationships were studied at seven sites of the brackish regions from March to October 2005 and 2006. POC concentration varied from 1.0 to $76.6mgL^{-1}$ (mean $7.4mgL^{-1}$), with maximal concentrations occurring in the middle parts of the study area in spring of 2005 and 2006. Concentrations of Chl-a and Pheo-a varied from 1.3 to $942.9{\mu}gL^{-1}$ (mean $71.0{\mu}gL^{-1}$) and $1.4{\sim}1,545.5{\mu}gL^{-1}$ (mean $59.9{\mu}gL^{-1}$), respectively, and corresponded closely with variation in POC. During the study period Pheo-a concentration was 44.2% of total Chl-a, implying that non-living or inactive phytoplankton is also the important part of phytoplankton-derived POC in brackish regions of Lake Sihwa. From the positive linear relationships between POC and phytoplankton pigments (POC with Chl-a (r=0.93), total Chl-a (r=0.88), and Pheo-a (r=0.81)), it is suggested that phytoplankton was a significant component of POC in the upper regions of brackish Lake Sihwa. On the other hand, the ratios of POC/Chl-a and POC/total Chl-a (Chl-a+Pheo-a) were 82.9 and 35.9, respectively. The ratio of POC/total Chl-a is similar to those reported in previous studies, including 40~60 in estuaries. This study suggests that Pheo-a concentration is considered in estimation of POC concentration from phytoplankton pigments in aquatic systems with high content of Pheo-a, like an upper region of blackish Lake Sihwa.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.26 no.3
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• pp.220-237
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• 2021

Since the functional importance of marine phytoplankton was firstly advocated from early 1880s massive data on the species composition and abundance were produced by classical microscopic observation and the advanced auto-imaging technologies. Recently, pigment composition resulted from direct chemical analysis of phytoplankton samples or indirect remote sensing could be used for the group-specific quantification, which leads us to more diversified data production methods and for more improved spatiotemporal accessibilities to the target data-gathering points. In quite a few cases of many long-term marine ecosystem monitoring programs the phytoplankton species composition and abundance was included as a basic monitoring item. The phytoplankton data could be utilized as a crucial evidence for the long-term change in phytoplankton community structure and ecological functioning at the monitoring stations. Usability of the phytoplankton data sometimes is restricted by the differences in data producers throughout the whole monitoring period. Methods for sample treatments, analyses, and species identification of the phytoplankton species could be inconsistent among the different data producers and the monitoring years. In-depth study to determine the precise quantitative values of the phytoplankton species composition and abundance might be begun by Victor Hensen in late 1880s. International discussion on the quality assurance of the marine phytoplankton data began in 1969 by the SCOR Working Group 33 of ICSU. Final report of the Working group in 1974 (UNESCO Technical Papers in Marine Science 18) was later revised and published as the UNESCO Monographs on oceanographic methodology 6. The BEQUALM project, the former body of IPI (International Phytoplankton Intercomparison) for marine phytoplankton data QA/QC under ISO standard, was initiated in late 1990. The IPI is promoting international collaboration for all the participating countries to apply the QA/QC standard established from the 20 years long experience and practices. In Korea, however, such a QA/QC standard for marine phytoplankton species composition and abundance data is not well established by law, whereas that for marine chemical data from measurements and analysis has been already set up and managed. The first priority might be to establish a QA/QC standard system for species composition and abundance data of marine phytoplankton, then to be extended to other functional groups at the higher consumer level of marine food webs.

• Korean Journal of Remote Sensing
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• v.36 no.6_1
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• pp.1339-1348
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• 2020

Phytoplankton controls marine ecosystems in terms of nutrients, photosynthetic rate, carbon cycle, etc. and the degree of its influence on the marine environment depends on their physical size. Many studies have been attempted to identify marine phytoplankton size classes using the remote sensing techniques. One of successful approach was the three-component model which estimates the chlorophyll concentrations of three phytoplankton size classes (micro-phytoplankton; >20 ㎛, nano-; 2-20 ㎛ and pico-; <2 ㎛) as a function of total chlorophyll. Here, we examined the applicability of Geostationary Ocean Colour Imager (GOCI) to the mapping of the phytoplankton size class distribution in the East Sea. A fit of the three-component model to a biomarker pigment dataset collected in the study area for some years including a large harmful algal bloom period has been carried out to derive size-fractioned chlorophyll concentration (CHL). The tuned three-component model was applied to the hourly GOCI images to identify the fractions of each phytoplankton size class for the entire CHL. Then, we investigated the distribution of phytoplankton community in terms of the size structure in the East Sea during the harmful Cochlodinium polykrikoides blooms in the summer of 2013.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.1
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• pp.27-41
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• 2008

Distribution characteristics of phytoplankton community were investigated by HPLC and flow cytometry in Jeju Strait and the Northern East China Sea (NECS) in May 2004, in order to understand the relationship between physical environmental factors and distribution pattern of phytoplankton communities. Based on temperature and salinity data, three distinct water masses were identified; warm and saline Tsushima Warm Current (TWC), which is flowing from northwest of Jeju Island, warm and low saline water at the center of Jeju Strait, which is originated from China Coastal Water (CCW) and relatively cold and high saline water originated from Yellow Sea at the bottom of the Jeju Strait. At Jeju Strait, less saline water (<33 psu) of 15 km width occupied surface layer up to 20 m which located at 20 km offshore and strong thermal front between warm and saline water and cold and less saline water was found in the middle of the Jeju Strait. Vertical transect of temperature and salinity at the NECS also showed that low saline (<33 psu) water occupied the upper 20 m layer and cold and saline water was present at the eastern part. Chl a was measured as $0.06{\sim}3.07\;{\mu}g/L$. Spring bloom of phytoplankton was recognized by the high concentrations of Chl a at the low saline water masses influenced by the CCW and subsurface chlorophyll maximum layer appeared between $20{\sim}30\;m$ depth, which was at thermocline depth or below. Abundances of Synechococcus and picoeukaryote were $0.2{\sim}9.5{\times}10^4\;cells/mL$ and $0.43{\sim}4.3{\times}10^4\;cells/mL$, respectively. Dinoflagellate, diatom and prymnesiophyte were major groups and minor groups were chlorophyte+prasinophyte, chrysophyte, cryptophyte and cyanophyte. Especially high abundance of dinoflagellate was identified by high concentration (>1\;{\mu}g/L$) of peridinin at the bottom of the thermocline, which showed an outbreak of red tide by high density of dinoflagellates. Abundances of picoeukaryote in Jeju Strait were about $5{\sim}10$ times higher than abundance measured in Kuroshio water and showed a good correlation with Chl b (Pras+Viola), which implies the most of population of picoeukaryote was composed of prasinophytes. Prochlorococcus was not detected at all, which suggests that Kuroshio Current did not directly influenced on the study area. Based on the strong negative correlations between biomass of phytoplankton (Chl a) and temperature+salinity, the primary production and biomass of phytoplankton in the study area were controlled by the nutrients supply from CCW.