• Title/Summary/Keyword: 식물인식

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A Role of Indole-3-acetic Acid on the Growth and the Accumulation of Tannin in Callus Induced from Gallas of the Sumac (붉나무충영의 배양조직에서 생육 및 tannin 축적에 미치는 indole-3-acetic acid의 역할)

  • Xu, Ming-Zi;Oh, In-Suk;So, Sang-Sup
    • Korean Journal of Environmental Biology
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    • v.19 no.4
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    • pp.248-253
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    • 2001
  • These studies were carried out to examine the developmental process of galls caused by aphids in Rhus gavanica and the effects of indole-3-acetic acid (IAA) and ${\alpha}$-naphthalene acetic acid (NAA) on the tannin accumulation in the callus induced from that galls. The results are follows. The development of fist-shaped galls has begun at the beginning of June earlier than the case of finger-shaped galls, and also, the growth of fist-shaped galls has last to September longer than the period of finger-shaped galls. These results indicate that the life cycle and feeding activity of the aphids inhabited in fist-shaped galls were longer and mire active than the case of the aphids inhabited in finger-shaped galls. Tannin contents of fist-shaped galls revealed about 60${\sim}70%$ of total dry weights during the whole growth periods, however, the contents in finger-shaped galls were under 10% at the maximum value. These facts mean that finger-shaped galls seem to be as a habitat of aphids rather than as a major source of tannin such as fist-shaped galls. The growth of callus induced from fist-shaped galls was the most effective in the plot of $10^{-5}$ mole IAA, but the tannin accumulation in callus growth was not even caused in any plots of IAA treatments as wells as in any NAA plots. These results considered that the tannin accumulation in fist-shaped galls may be caused only in specific relation between host plant and life cycle of aphids.

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Simultaneous Effect of Salinity and Temperature on the Neutral Lipid and Starch Accumulation by Oceanic Microalgae Nannochloropsis granulata and Chlorella vulgaris (염분과 온도의 동시 영향에 따른 해양 미세조류 Nannochloropsis granulata와 Chlorella vulgaris의 중성지질 및 녹말 축적에 관한 연구)

  • Ko, Kyungjun;Lee, Chi-Heon;Moon, Hye-Na;Lee, Yeon-Ji;Yang, Jinju;Cho, Kichul;Kim, Daekyung;Yeo, In-Kyu
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.19 no.3
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    • pp.236-245
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    • 2016
  • Because microalgae represent high growth rate than terrestrial plants, and it can accumulate significant lipid and carbohydrate content, and other bioactive compounds such as carotenoid and polyphenol in their body, it has been considered as one of the promising resources in bio-energy, and other industries. Although many studies has been performed about the microalgae-derived biochemical accumulation under various abiotic conditions such as different temperatures, salinities and light intensities, the studies about simultaneous effect of those parameters has rarely been performed. Therefore, this study focused on evaluation of simultaneous effect of different salinity (10, 30, 50 psu) and temperatures (20, 25, $30^{\circ}C$) on the changes of biomass, lipid, starch and photosynthetic pigment accumulation. As results, the highest growth rate was achieved at $30^{\circ}C$ and 30 psu in the both algal cultures, and the photosynthetic pigment, chlorophyll a and total carotenoid content, were increased in a temperature-dependent manner. The accumulation of lipid and starch contents exhibited different aspects under different combinations of temperature and salinity. From the results, it is suggested that the changes of microalgal lipid and starch accumulation under different salinities may be affected by the different temperatures.

Process Development for Production of Antioxidants from Lipid Extracted Microalgae Using Ultrasonic-assisted Extraction (탈지미세조류로부터 초음파추출을 이용한 항산화 물질 생산 공정 최적화)

  • Jo, Jaemin;Shin, Suelgl;Jung, Hyunjin;Min, Bora;Kim, Seungki;Kim, Jinwoo
    • Korean Chemical Engineering Research
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    • v.55 no.4
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    • pp.542-547
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    • 2017
  • Ultrasound-assisted extraction (UAE) has attracted growing interest, as it is an effective method for the rapid extraction of bioactive compounds from plants with a high extraction efficiency comparable to the conventional extraction. In this study, UAE was used for the extraction of polyphenols from lipid extracted microalgae (Tetraselmis KCTC 12236BP) and the effects of five extraction variables on the total phenolic compounds (TPC) were studied. For the optimization of extraction parameters, particle size, solid-to-liquid (L/S) ratio, ethanol concentration, extraction temperature and extraction time have been examined as independent variables. All variables exhibited the significant effects on the extraction of TPC and extraction temperature showed the most significant effect among five variables. The optimal extraction conditions were the extraction using mixed particle, S/L ratio of 10%, ethanol concentration of 60%, extraction temperature of $100^{\circ}C$ and extraction time of 30 min, which gave the 8.7 mg GAE/g DW for TPC. Compared with conventional hot-water extraction, TPC extraction under UAE was increased by up to 1.8 fold with same extraction condition. This study showed that UAE under low temperature and short extraction time was proven to be an effective extraction process for TPC production from LEA compared to conventional hot-water extraction process.

Change of Fish Assemblage with Altered Flow Regime in Geum River (금강에서의 유량변동과 어류상 변화)

  • Seo, Jin-Won;Hong, Young-Pyo;Kim, Jeong-Kon;Park, Sang-Young;Kim, Gee-Hyoung;Ko, Ick-Hwan
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.612-616
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    • 2007
  • 오랫동안 홍수조절, 용수공급, 전력생산, 레저 활동을 위해 건설된 댐들은 하천이나 호소의 생태적 기능보다는 치수 및 이수기능이 중요시되면서 운영 관리되어 왔다. 하지만 최근 들어 선진국을 중심으로 환경 및 생태의 중요성이 인식되면서 하천생태계 보호를 위한 환경유량 산정이 언급되고 있는데 이는 하천이 동 식물의 서식처로서 적절한 수질뿐만 아니라 수심, 유속, 하상재료, 먹이원, 어류를 보호할 수 있는 휴식 및 은신처 등의 다양한 조건을 제공할 수 있어야 한다고 제시되고 있다. 이들 조건 중 유량(Flow)은 하천에서 생물집단을 구성하는데 있어 물리적 서식조건의 주요한 결정인자로 작용하는데, 댐 하류하천의 자연유량(natural flow)을 변화시키면 생태학적 측면에서 기대치 않던 영향을 야기할 수 있다. 따라서 댐 건설 전 후의 자연유량과 조절유량 사이에서 생태학적 어류조사연구를 통한 차이점을 찾는 것은 매우 중요하다고 볼 수 있다. 본 연구의 최종목적은 수계 내 댐 건설 전 후의 어류군집과 유량 등 수리조건과의 연관성을 분석하여 하천구간별로 어류생태에 적합한 유량을 산정하는 것이다. 조사대상 구간은 금강본류를 대상으로 하되 용담댐과 대청댐을 중심으로 하여 10개 구간을 선정하였고. 과거로부터 2000년도까지와 용담댐 건설후인 $2002{\sim}2004$년도에 조사된 어류생태자료를 활용하였다. 용담댐 상류로부터 대청댐 하류에 이르기까지 전 구간을 대상으로 조사된 자료를 분석한 결과 총 20과 82종이 출현하였고, 이중 종 45종(54.9%)이 잉어과에 해당하며 미꾸리과(6종), 동자개과(4종), 망둑어과(5종)를 제외한 대다수의 분류군(Family)들은 단일 또는 2종으로만 구성되었다. 이들 중 천연기념물 제 259호로 지정되어 법적으로 보호되고 있는 어름치(금강에서 서식한 어름치를 따로 천연기념물 238호로 지정함)를 비롯하여 다묵장어를 포함한 7종의 멸종위기종이 확인되었다. 또한 각시붕어 등 총 29종(39.4%)의 높은 한국고유종 출현과 6종의 외래도입종도 확인되었다. 금강의 중 상류에 해당하는 구역을 포함하는 대청댐 상 하류 구간에서 대청댐 건설 전(1980년 이전)을 포함하는 2000년 이전 조사 자료가 가장 다양한 어류상과 특이어종(멸종위기종 및 한국고유종)을 보였고, 최근자료$(2002{\sim}2004)$를 살펴볼 때 용담댐 상 하류에서보다 대청댐 상 하류에서 멸종위기종(7종$\rightarrow$1종) 및 한국고유종(28종$\rightarrow$16종)의 출현감소와 외래도입종의 출현증가(1종$\rightarrow$6종)와 같은 주요한 어류군집 변화를 보였다. 이는 댐 건설에 따라 주로 계류성 어종을 중심으로 정수역 구간에서 인근 지류로 이동하게 되고 일부는 제한된 서식공간과 하천교란으로 인해 개체군이 극감하거나 일부 소멸된 종이 발생하였을 것으로 사료된다. 실측 유량자료 분석에 의하면 홍수기 최대유량 변화는 크지 않으나, 갈수기 최소유량은 크게 증가한 것을 알 수 있었고, 대다수 어류의 산란기인 봄철(5월, 6월) 최소유량은 증가하였으나, 최대유량은 감소한 것으로 분석되었다. 이러한 변화는 어류생태계에 긍정적인 면과 부정적인 면이 있으며, 향후 이러한 영향을 분석하기 위해 선택어종(Target species)별 생태환경 서식조건을 확인할 수 있는 조사기법 및 자료구축이 절실히 필요한 실정이다.

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Analysis of Elementary School Students' Psychological Science Domains by Classifying Science Vocabularies. (과학 용어 분류를 통한 초등학생들의 심리적 과학 영역 분석)

  • Lee, Sung-Ho;Lim, Cheong-Hwan;Jeong, Jin-Woo
    • Journal of The Korean Association For Science Education
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    • v.21 no.1
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    • pp.30-37
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    • 2001
  • The importance of integrated approach have increased gradually in science education. But most of researches on integrated science have been based on the structure of science and epistemology. The investigations of students' psychological science domains are needed for effectiveness of integrated science in science curriculum and classrooms. In this study, for exploration of elementary school students' psychological science domains and their properties, students(N=96) were asked to classify scientific words presented on textbooks in several categories. Many psychological science domains were revealed. Among them, the domains that were over 20% are 'Electricity', 'The human body', 'The earth', 'Energy', 'Plant', 'Animal', 'Environment', 'Living things', 'Weather', 'Flower'. According to the results from analysis on the words that students presented in each domains, the participants hold strongly the relationships caused by everyday experiences. For example, 'light' and 'heat' showed high frequency in 'Electricity' domain and 'The human body' domain included 'force' and 'motion' in high frequency. The most inclusive domain was 'The earth'. Not only the words on earth science but also biological and chemical words were classified in this domain. So The integration that centered with 'The earth' domain looks correspond to the elementary school students' psychological aspect.

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Research trends, applications, and domestic research promotion stratigies of metabolomics (대사체학의 연구 동향, 응용 및 국내 연구 활성화 방안)

  • Kim, So-Hyun;Yang, Seung-Ok;Kim, Kyoung-Heon;Kim, Young-Suk;Liu, Kwang-Hyeon;Yoon, Young-Ran;Lee, Dong-Ho;Lee, Choong-Hwan;Hwang, Geum-Sook;Chung, Myeon-Woo;Choi, Ki-Hwan;Choi, Hyung-Kyoon
    • KSBB Journal
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    • v.24 no.2
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    • pp.113-121
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    • 2009
  • As one of the new areas of 'omics' technology, there is increasing interest in metabolomics, which involves the analysis of low-molecular-weight compounds in cells, tissues, and biofluids, and considers interactions within various organisms and reactions of external chemicals with those organisms. However, metabolomics research is still at a fundamental stage in Korea. Therefore, the purpose of this study was to establish a strategic long-term plan to revitalize the national metabolomics approach and obtain the elementary data necessary to determine a policy for effectively supporting metabolomics research. These investigations clarified the state of metabolomics study both in Korea and internationally, from which we attempted to find the potentiality and fields where a metabolomics approach would be applicable, such as in medical science. We also discuss strategies for developing metabolomics research. This study revealed that promoting metabolomics in Korea requires cooperation with metabolomics researchers, acquisition of advanced technology, capital investment in metabolomics approach, establishment of metabolome database, and education of metabolome analysis experts. This would reduce the gap between the national and international levels of metabolomics research, with the resulting developments in metabolomics having the potential to greatly contribute to promoting biotechnology in Korea.

Quantification and Varietal Variation of Rutin in Mulberry Fruits (뽕나무 계통별 오디함유 rutin 분석 및 품종변이)

  • Kim Hyun-bok;Kim Sun-lim
    • Journal of Sericultural and Entomological Science
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    • v.46 no.1
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    • pp.1-5
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    • 2004
  • Rutin has many beneficial effects on human health. It was established that rutin antagonizes the increase of capillary fragility associated with hemorrhagic disease, reduces high blood pressure, decreases the permeability of the vessels, and has an antiedema effect, reduces the risk of arteriosclerosis, and shows antioxidant activity. Rutin analysis with mulberry fruits was carried out by high-performance liquid chromatography (HPLC) to reveal the possibility of source of functional food. As a result, mean content of fifty accessions was 0.14±0.050% DW. Among the tested fifty accessions, 'Sabangso' was showed the highest rutin in 0.29%DW, whereas 'Simseol' was the lowest content in 0.05% DW. Also, we researched the change of rutin content according to harvested date. The rutin contents of earlier harvested groups were higher than later harvested groups. Simultaneously, fruity characteristics as well as rutin content were researched and analyzed to select the functional mulberry varieties for the production of fruit. From the six accessions which were contained high rutin content, we selected three suitable varieties such as 'Ficus', 'Kangsun', and 'Palcheongsipyung'.

Science Experience's Type and Meaning of Korean Middle School-Science Gifted Students in Parent.School.Out-of-School Institution (중학교 과학 영재들의 부모.학교.학교 밖 교육 기관에서의 과학 경험의 유형과 그 의미)

  • Choi, Yunhee;Choi, Kyunghee
    • Journal of The Korean Association For Science Education
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    • v.32 no.10
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    • pp.1580-1598
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    • 2012
  • The Study aimed to observe Science Experience's Type in parent school out of school institution and Meaning of Middle School- Science Gifted Students in Korea. Twelve middle school gifted education institution and science high school students participated in this study. Data source was retrospective and in-depth interviews with individuals. Results were as follows. First, science experience with parents influenced interest and inquiring mind in science, choice of career in field of science. Second, science experience in school curriculum recognized that gifted students have talents of themselves in science and changed in Attitude toward science from science teacher. Third, science experience with education institution for out of school curriculum changed interest in science from Experiment for identified scientific knowledge and obtained new product through creative inquiry process. Furthermore, It was gained self-confidence, feel a sense of accomplishment and a challenging, the spirit of team work including of taking lead the team.

Introduction process of 'Corn' and its interrelation with 'Chinese millet' and 'Indian millet' (옥수수(옥촉서(玉蜀黍))의 도입과정과 기장(태(泰)), 수수(촉서(蜀黍))와의 상관관계)

  • Kim, Jong-dug;Koh, Byung-hee;Song, Il-byung
    • Journal of Sasang Constitutional Medicine
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    • v.10 no.2
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    • pp.163-180
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    • 1998
  • In 1492 year, the corn originated in America continent had spread all over the world after spreading into Europe by Columbus. Since the Corn had a similar shape with adlai's one (Yulmoo(율무)), it had been written by the different name of adlai (Yulmoo) at the "訓蒙字會(Hun-mong-ja-hoe)(1527)". Therefore we should consider "Hun-mong-ja-hoe" is the first record of the corn and it is a significant historic record in the civilization exchange between the Orient and the Occident that this record has the only difference of around 30 years later after the spreading corn to Europe. However, this is on the assumption that it is correct for a scholar of Korean literature to persist in that '叡山本' of "Hun-mong-ja-hoe" could be considered as the first edition. The corn had been once classified as a same kind of the Chinese millet because the people had been recognized the corn as a similar group of the Chinese millet and the Indian millet. The Chinese millet contains a summer vigor and becomes as an ingredient of alcoholic drink. And we can find out that $C_4$ type plant (such as corn, Chinese millet, Indian millet, foxtail millet) mostly have a tendency to belong to the food for "Taeumin(太陰人)", because of its high energy efficiency, a flourishing absorption of fertilizing and a strong emission power. The fried corn with a strong summer vigor and a raised feature has a good effect to the treatment of the teethridge disease. And the tea of corn with an warm feature, thanks to its functions of making the stomach and intestines comfortably as well as urination, is a proper food for the 'Taeumin" who is apt to overeat themselves.

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Antifungal Activity of Phenanthrene Derivatives from Aerial Bulbils of Dioscorea batatas Decne (재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성)

  • Kum, Eun-Joo;Park, Sang-Jo;Lee, Bong-Ho;Kim, Jong-Sik;Son, Kun-Ho;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.16 no.4
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    • pp.647-652
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    • 2006
  • Plants of the genus Dioscorea have long been used as oriental folk medicine, and Dioscorea batatas Decne has been cultivated for healthy food in Korea. Although the bulbils were produced 2,000 ton annually, there are few reports for bioactive compounds in bulbils. In this study, three phenanthrenes and two phenanthraquinones were isolated from the aerial bulbils of D. batatas Decne, and their structures were elucidated. Among them, compound 2 (6-hydroxy-2,7-dimethoxy-1,4-phenanthraquinone) has not been reported previously. Evaluation of antimicrobial activities based on disk-diffusion assay, MIC and MFC showed the compound 12 (6,7-dihydroxy-2,4-dimethoxyphenanthrene) has strong antimicrobial activity with $25\;{\mu}g/ml$ of MIC and MFC against Candida albicans. Our results suggested that compound 12 has a potent antifungal activity, and the antimicrobial activity and its spectrum are modulated by hydroxylation and methoxylation of phenanthrene ring moiety of the compound.